Current Affiliation Organization 【 display / non-display 】

Current Affiliation Organization 【 display / non-display 】

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External Career 【 display / non-display 】

Research Interests 【 display / non-display 】

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Research Areas 【 display / non-display 】

Research Areas 【 display / non-display 】

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Research Theme 【 display / non-display 】

Research Theme 【 display / non-display 】

• Screening of Anti-Cancer Compound from Subtropical Resources

• Isoprene Emission from Tropical Trees

• Screening of Stress Related Lipid Component

Published Papers 【 display / non-display 】

Published Papers 【 display / non-display 】

• Molecular characteristics of isoprene synthase and its control effects on isoprene emissions from tropical trees.

  Oku H, Mutanda I, Inafuku M

  Journal of plant research   136 ( 1 ) 63 - 82   2023.01 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Fractionation and characterization of cell wall polysaccharides from the brown alga Cladosiphon okamuranus.

  Awanthi MGG, Umosa M, Yuguchi Y, Oku H, Kitahara K, Ito M, Tanaka A, Konishi T

  Carbohydrate research   523   108722   2023.01 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Stress amelioration and anti-inflammatory potential of Shiikuwasha (Citrus depressa Hayata) essential oil, limonene, and γ-terpinene

  Yonathan Asikin, Kuniyoshi Shimizu, Hironori Iwasaki, Hirosuke Oku, Koji Wada

  Journal of Food and Drug Analysis ( The Journal of Food and Drug Analysis (JFDA), Food and Drug Administration, Taiwan (TFDA) )  30 ( 3 ) 454 - 465   2022.09 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Isolation and characterization of mimosine degrading enzyme from Arthrobacter sp. Ryudai-S1.

  Shigeki Oogai, Masakazu Fukuta, Masashi Inafuku, Hirosuke Oku

  World journal of microbiology & biotechnology   38 ( 10 ) 172 - 172   2022.07 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

  　View Summary

  Leucaena leucocephala growing in the tropics and subtropics serves as potential forage for livestock because its foliage is rich in protein, fiber, and minerals. However, its use for livestock feed has been hindered by toxic nonprotein amino acid mimosine. Therefore, it is necessary to develop a method to reduce or eliminate mimosine from foliage. A previous study found that the fermentation of L. leucocephala foliage reduced the mimosine content and prompted the authors to isolate potent mimosine degrading microorganisms and characterize the mimosinase for the complete elimination of mimosine in the L. leucocephala foliage. The soil screening of the L. leucocephala tree surroundings led to the isolation of Arthrobacter sp. Ryudai-S1, which can degrade and assimilate mimosine as a nitrogen and carbon source. Mimosinase in this strain was found to be thermostable and showed strong activity. Docking model's inspection and the interaction energy calculation between mimosine-pyridoxal-5'-phosphate (PLP) complex and the active site of this enzyme identified 11 important amino acid residues that stabilized the binding. Of these amino acid residues, mutation experiment suggested that Tyr-263' and Phe-34 stabilizes the substrate binding and play a critical role in guiding the substrate to proper positions to accomplish high catalytic efficacy and selectivity. These observations suggest that Arthrobacter sp. Ryudai-S1 could be potentially useful for the development of L. leucocephala feed with reduced mimosine content.

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• Plant hormone profile and control over isoprene biosynthesis in a tropical tree Ficus septica

  Md A. Iqbal, K. Miyamoto, E. Yumoto, S. Parveen, I. Mutanda, M. Inafuku, H. Oku

  Plant Biology   24 ( 3 ) 492 - 501   2022.04 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

  　View Summary

  Plant hormone signalling and the circadian clock have been implicated in the transcriptional control of isoprene biosynthesis. To gain more insight into the hormonal control of isoprene biosynthesis, the present study measured plant hormone concentrations in jasmonic acid (JA)-treated leaves of our previous model study, examined their relationship with gene expression of isoprene synthase (IspS) and hormone signalling transcription factors. Of the plant hormones, IAA and JA-Ile and their related transcription factors (MYC2 and SAUR21) were significantly correlated with IspS gene expression. Concentrations of cytokinins, isopentenyladenine (iP), trans-zeatin riboside (tZR) and cis-zeatin riboside (cZR), were similarly significantly correlated with IspS expression. However, there was no significant correlation between their related transcription factor (ARR-B) and IspS expression. The circadian clock-related gene PRR7, but not the transcription factor LHY, was highly correlated with IspS expression. These results suggest that the hormonal balance between JA-Ile and IAA plays a central role in transcriptional regulation of IspS through the transcription factors MYC2 and SAUR21, the early auxin responsive genes. The putative cis-acting elements for SAUR on the IspS promoter (TGTCNN and CATATG), in addition to the G-box for MYC2, support the above proposal. These results provide insightful information on the core components of plant hormone-related regulation of IspS under coordination with the circadian clock genes.

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Books 【 display / non-display 】

Books 【 display / non-display 】

• Approaches in enhancing abiotic stress tolerance to plants

  Mirza Hasanuzzaman, M. Fujita, H. Oku, Md. Tofazzal Islam ( Part: Editing and Writing ,  Approaches in enhancing abiotic stress tolerance to plants )

  Taylor & Francis. CRC Press  2018.12

• Plant Nutrients and Abiotic Stress Tolerance

  Mirza Hasanuzzaman, M. Fujita, H. Oku, K. Nahar, B. Hawrylak-Nowak ( Part: Editing and Writing )

  2018.12

• Plant Tolerance to Environmental Stress: Role of Exogenous Phytoprotectants.

  Mirza Hasanuzzaman, M. Fujita, H. Oku, Md. Tofazzal Islam ( Part: Editing and Writing ,  Role of Exogenous Phytoprotectants )

  Taylor & Francis. CRC Press  2018.12

• Reactive Oxygen Species and Antioxidant Systems in Plants: Role and Regulation under Abiotic Stress

  Hasanuzzaman, M, Al Mahmud, J, Nahar, K, Anee, T.I, Inafuku, M, Oku, H, Fujita, M ( Part: Multiple Authorship ,  Plant responses, adaptation and ROS metabolism in plants exposed to waterlogging stress )

  Springer Singapore  2017.08

• Phytohormones and Their Signaling Mechanisms in Plant Development

  Mirza Hasanuzzaman, Kamrun Nahar, Farha Tasnim Bhuiyan, Taufika Islam Anee, Hirosuke Oku, Masayuki Fujita ( Part: Multiple Authorship ,  Salicylic acid: an all-rounder in regulating abiotic stress responses in plant )

  Intech, Rijeka  2017.08

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Other Papers 【 display / non-display 】

Other Papers 【 display / non-display 】

• 健康長寿に有用活用可能な沖縄・奄美伝統の機能性食品素材のエビデンス 肥満関連病態に対する長命草・ボタンボウフウの機能性

  稲福征志, 岡部貴史, 屋宏典

  機能性食品と薬理栄養   13 ( 2 ) 68‐75   2019.10

   

  J-GLOBAL

• ギンネムのミモシン合成酵素の同定と酵素学的性質の解明

  大貝茂希, 福田雅一, 渡邊啓一, 屋宏典

  日本農芸化学会大会講演要旨集(Web)   2019   ROMBUNNO.4E7a13 (WEB ONLY)   2019.03

   

  J-GLOBAL

• 抗肥満活性を有するうちなー野菜の探索

  中村海太, 稲福征志, 前田剛希, 広瀬直人, 玉城盛俊, 高江洲賢文, 砂川春樹, 屋宏典, 屋宏典

  日本農芸化学会中四国支部講演会講演要旨集(Web)   49th   122 (WEB ONLY)   2017.09

   

  J-GLOBAL

• 熱帯樹木のイソプレン放出制御におけるイソプレン合成酵素の生理学的意義

  比嘉拓也, 稲福征志, 屋宏典, 屋宏典

  日本農芸化学会中四国支部講演会講演要旨集(Web)   49th   106 (WEB ONLY)   2017.09

   

  J-GLOBAL

• 沖縄県産長命草(ボタンボウフウ)由来ジヒドロピラノクマリンとその抗肥満活性

  中村海太, 稲福征志, 屋宏典

  日本栄養・食糧学会大会講演要旨集 ( (公社)日本栄養・食糧学会 )  71st   360 - 360   2017.04

   

  J-GLOBAL

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Presentations 【 display / non-display 】

Presentations 【 display / non-display 】

• 植物由来ミモシナーゼの反応機構及びシスタチオニンβ‐リアーゼとの分子進化的関係の解明

  大貝茂希, 福田雅一, 渡邊啓一, 屋宏典

  日本農芸化学会大会講演要旨集(Web)  1900.01  -  1900.01 

• ギンネムのミモシン合成酵素の同定と酵素学的性質の解明

  大貝茂希, 福田雅一, 渡邊啓一, 屋宏典

  日本農芸化学会大会講演要旨集(Web)  1900.01  -  1900.01 

• Isolation and phylogenetic analysis of new predicted polyprenol reductase from mangrove plant (Kandelia obovata Sheue, H. Y. Liu & J. Yong)

  Basyuni, M, Baba, S, Wati, R, Sumardi, Sulistiyono, N, Oku, H, Sagami, H

  AIP Conference Proceedings  1900.01  -  1900.01 

• Characterization of cycloartenol synthase KcCAS promoter region from mangrove plant (Kandelia obovata Sheue, H. Y. Liu & J. Yong)

  Basyuni, M, Wati, R, Baba, S, Oku, H

  AIP Conference Proceedings  1900.01  -  1900.01 

• 熱帯樹木のイソプレン放出制御におけるイソプレン合成酵素の生理学的意義

  比嘉拓也, 稲福征志, 屋宏典, 屋宏典

  日本農芸化学会中四国支部講演会講演要旨集(Web)  1900.01  -  1900.01 

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Grant-in-Aid for Scientific Research 【 display / non-display 】

Grant-in-Aid for Scientific Research 【 display / non-display 】

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2015.04  -  2018.03 

  Direct: 3,600,000 (YEN)  Overheads: 1,080,000 (YEN)  Total: 4,680,000 (YEN)

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2011.04  -  2013.03 

  Direct: 3,900,000 (YEN)  Overheads: 1,170,000 (YEN)  Total: 5,070,000 (YEN)

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2008  -  2010 

  Direct: 1,600,000 (YEN)  Overheads: 480,000 (YEN)  Total: 2,080,000 (YEN)

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2005  -  2006 

  Direct: 1,500,000 (YEN)  Total: 1,500,000 (YEN)

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2003.04  -  2005.03 

  Direct: 2,300,000 (YEN)  Overheads: 690,000 (YEN)  Total: 2,990,000 (YEN)