Current Affiliation Organization 【 display / non-display 】

Current Affiliation Organization 【 display / non-display 】

Published Papers 【 display / non-display 】

Published Papers 【 display / non-display 】

• Biomarkers for Predicting Anti-Programmed Cell Death-1 Antibody Treatment Effects in Head and Neck Cancer.

  Tanaka K, Hirakawa H, Suzuki M, Higa T, Agena S, Hasegawa N, Kawakami J, Toyama M, Higa T, Kinjyo H, Kise N, Kondo S, Maeda H, Ikegami T

  Current oncology (Toronto, Ont.) ( Current Oncology )  30 ( 6 ) 5409 - 5424   2023.06 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Human Papillomavirus Infection and EGFR Exon 20 Insertions in Sinonasal Inverted Papilloma and Squamous Cell Carcinoma.

  Hirakawa H, Ikegami T, Kise N, Kinjyo H, Kondo S, Agena S, Hasegawa N, Kawakami J, Maeda H, Suzuki M

  Journal of personalized medicine ( Journal of Personalized Medicine )  13 ( 4 ) 657   2023.04 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Brain Activity in Patients With Unilateral Vocal Fold Paralysis Detected by Functional Magnetic Resonance Imaging.

  Kiyuna A, Kise N, Hiratsuka M, Maeda H, Hirakawa H, Ganaha A, Suzuki M

  Journal of voice : official journal of the Voice Foundation ( Journal of Voice )  36 ( 5 ) 738.e1 - 738.e9   2022.09 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  OBJECTIVES: Understanding brain activity in response to unilateral vocal fold paralysis is essential to determine the neural compensatory mechanism underlying adaptation to voice disorders and to develop novel and improved rehabilitation programs for these disorders. We aimed to clarify brain activity during phonation (prolonged vowel, |i:|) in patients with chronic left vocal fold paralysis (LVFP) and compare with that in normal controls. STUDY DESIGN: Case-control study. METHODS: This functional magnetic resonance imaging (fMRI) study of an event-related task comprised 12 individuals with LVFP of more than 6 months duration and 12 healthy controls. The experimental task alternated phonation (prolonged vowel, |i:|) and no phonation (rest) conditions. The functional images obtained were single-shot gradient-echo echo-planar imaging. The volumes were acquired parallel to the anterior-posterior commissure plane and were sensitive to BOLD contrast. Data sets were processed and statistically analyzed using Statistical Parametric Mapping 8 software. Within-group analyses were conducted by applying the one-sample t test (P < 0.001, uncorrected). A random-effects analysis was used for group comparison. RESULTS: The LVFP group showed significantly higher brain activity in the right premotor areas, left parietal lobule, right primary somatosensory areas, and bilateral supplementary motor area and lower brain activity in the auditory-related areas of the superior temporal gyrus. There were no significant correlations of the percent signal change on fMRI with disease duration, maximum phonation time, or age. CONCLUSION: Patients with chronic unilateral vocal fold paralysis have stronger activity during voluntary phonation in various central networks. More detailed information on the central nervous system regions related to voluntary phonation from early to chronic phase is needed to understand the compensatory mechanisms in vocal fold paralysis and to establish an effective rehabilitation program. This is the first report to investigate brain activity in chronic unilateral vocal fold paralysis.

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• The Spatio-temporal Gene Expression of Human Papillomavirus in Laryngeal Papilloma

  Ikegami Taro, Kiyuna Asanori, Kise Norimoto, Suzuki Mikio

  Koutou (THE LARYNX JAPAN) ( THE JAPAN LARYNGOLOGICAL ASSOCIATION )  33 ( 02 ) 69 - 75   2021.12 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  <p>Laryngeal papilloma (LP) is a tumor caused by infection of the larynx with human papillomavirus type 6 or 11 (HPV-6 or HPV-11) and is a multiple, recurrent, and refractory disease. HPV-6 and HPV-11 have nine genes, but the detailed functions of these genes in the formation of recurrent and refractory LP have not been elucidated. We examined the viral DNA load and mRNA expression as well as the <I>in situ</I> localization in HPV-6-infected LP. Our novel phage display method produced a monoclonal antibody against the viral E4 protein as <I>E4</I> mRNA was found to be the most highly expressed of the nine viral mRNAs. The average viral load was 4.80 × 10⁵ ± 1.86 × 10⁵ copies/ng DNA. <I>E4, E5a</I>, and <I>E5b</I> mRNAs accounted for 96% of the expression of the 9 mRNAs. The changes in the viral DNA load and expression of the nine mRNAs showed a similar curve during recurrence. The <I>E4, E5a</I>, and <I>E5b</I> mRNAs were co-expressed in the middle to the upper part of the epithelium. These results suggest that individual viral genes are coordinately expressed for viral replication, virus release, and immunosurveillance avoidance. Antibody against HPV-6 E4 shows high sensitivity and specificity in western blotting and immunohistochemistry and can be used for further functional studies and clinical applications.</p>

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• Development of Antibodies against HPV-6 and HPV-11 for the Study of Laryngeal Papilloma.

  Ikegami T, Kise N, Kinjyo H, Kondo S, Suzuki M, Tsukahara N, Murakami A, Kiyuna A, Agena S, Tanaka K, Hasegawa N, Kawakami J, Ganaha A, Maeda H, Hirakawa H

  Viruses ( Viruses )  13 ( 10 )   2021.10 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  Laryngeal papilloma (LP), which is associated with infection by human papillomavirus (HPV)-6 or -11, displays aggressive growth. The precise molecular mechanism underlying the tumorigenesis of LP has yet to be uncovered. Building on our earlier research into HPV-6, in this study, the viral gene expression of HPV-11 was investigated by quantitative PCR and DNA/RNA in situ hybridization. Additionally, newly developed antibodies against the E4 protein of HPV-6 and HPV-11 were evaluated by immunohistochemistry. The average viral load of HPV-11 in LP was 1.95 ± 0.66 × 105 copies/ng DNA, and 88% of HPV mRNA expression was found to be E4, E5a, and E5b mRNAs. According to RNA in situ hybridization, E4 and E5b mRNAs were expressed from the middle to upper part of the epithelium. E4 immunohistochemistry revealed a wide positive reaction in the upper cell layer in line with E4 mRNA expression. Other head and neck lesions with HPV-11 infection also showed a positive reaction in E4 immunohistochemistry. The distribution pattern of HPV DNA, viral mRNA, and E4 protein in LP with HPV-11 infection was quite similar to that of HPV-6. Therefore, it might be possible to apply these E4-specific antibodies in other functional studies as well as clinical applications, including targeted molecular therapies in patients with HPV-6 and HPV-11 infection.

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