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Published Papers 【 display / non-display 】

Published Papers 【 display / non-display 】

• A novel cell source for therapy of knee osteoarthritis using atelocollagen microsphere-adhered adipose-derived stem cells: Impact of synovial fluid exposure on cell activity

  Sakamoto, T; Fuku, A; Horie, T; Kitajima, H; Nakamura, Y; Tanida, I; Sunami, H; Hirata, H; Tachi, Y; Iida, Y; Yamada, S; Yamamoto, N; Shimizu, Y; Ishigaki, Y; Ichiseki, T; Kaneuji, A; Osawa, S; Kawahara, N

  REGENERATIVE THERAPY ( Regenerative Therapy )  27   408 - 418   2024.12 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  INTRODUCTION: Administration of adipose-derived stem cells (ADSCs) into the joint cavity has been shown to alleviate the symptoms of knee osteoarthritis (OA) by releasing exosomes and anti-inflammatory cytokines. However, the therapeutic effect of these cells is limited by their rapid disappearance after administration. Thus, it is necessary to prolong cell survival in the joint cavity. This study aimed to investigate the potential application of ADSCs adhered to atelocollagen microspheres (AMSs) for cell therapy of knee OA. METHODS: ADSCs were cultured for 2, 4, and 7 days in AMS suspension or adherent culture dishes. The supernatants were analyzed for IL-10 and exosome secretion via enzyme-linked immunosorbent assay and Nanosight. The effect of AMS was compared with that of adherent-cultured ADSCs (2D-cultured ADSCs) using transcriptome analysis. Moreover, the solubility of AMS and viability of ADSCs were evaluated using synovial fluid (SF) from patients with knee OA. RESULTS: Compared with 2D-cultured ADSCs, AMS-cultured ADSCs exhibited a significant increase in secretion of exosomes and IL-10, and the expression of several genes involved in extracellular matrix and immune regulation were altered. Furthermore, when AMS-cultured ADSCs were cultured in SF from knee OA patients to mimic the intra-articular environment, the SF dissolved the AMSs and released viable ADSCs. In addition, AMS-cultured ADSCs showed significantly higher long-term cell viability than 2D-cultured ADSCs. CONCLUSION: Increased survival of AMS-adhered ADSCs was observed in the intra-articular environment, and AMSs were found to gradually dissipate. These results suggest that AMS-adhered ADSCs are promising source for cell therapy of knee OA.

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• Subpial transplantation of adipose-derived stem cells alleviates paraplegia in a rat model of aortic occlusion/reperfusion-induced spinal cord infarction

  Takahara, E; Kamizato, K; Kakinohana, M; Sunami, H; Kise, Y; Furukawa, K; Ntege, EH; Shimizu, Y

  REGENERATIVE THERAPY ( Regenerative Therapy )  26   611 - 619   2024.06 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Optimizing mesenchymal stem cell extracellular vesicles for chronic wound healing: Bioengineering, standardization, and safety

  Shimizu, Y; Ntege, EH; Inoue, Y; Matsuura, N; Sunami, H; Sowa, Y

  REGENERATIVE THERAPY ( Regenerative Therapy )  26   260 - 274   2024.06 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  Chronic wounds represent a significant global burden, afflicting millions with debilitating complications. Despite standard care, impaired healing persists due to factors like persistent inflammation and impaired tissue regeneration. Mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) offer an innovative regenerative medicine approach, delivering stem cell-derived therapeutic cargo in engineered nanoscale delivery systems. This review examines pioneering bioengineering strategies to engineer MSC-EVs into precision nanotherapeutics for chronic wounds. Emerging technologies like CRISPR gene editing, microfluidic manufacturing, and biomimetic delivery systems are highlighted for their potential to enhance MSC-EV targeting, optimize therapeutic cargo enrichment, and ensure consistent clinical-grade production. However, key hurdles remain, including batch variability, rigorous safety assessment for potential tumorigenicity, immunogenicity, and biodistribution profiling. Crucially, collaborative frameworks harmonizing regulatory science with bioengineering and patient advocacy hold the key to expediting global clinical translation. By overcoming these challenges, engineered MSC-EVs could catalyze a new era of off-the-shelf regenerative therapies, restoring hope and healing for millions afflicted by non-healing wounds.

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• Exploring SSEA3 as an emerging biomarker for assessing the regenerative potential of dental pulp-derived stem cells

  Shirakawa, J; Ntege, EH; Takemura, M; Miyamoto, S; Kawano, T; Sampei, C; Kawabata, H; Nakamura, H; Sunami, H; Hayata, T; Shimizu, Y

  REGENERATIVE THERAPY ( Regenerative Therapy )  26   71 - 79   2024.06 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Adipose-derived stem cell therapy for spinal cord injuries: Advances, challenges, and future directions

  Shimizu, Y; Ntege, EH; Takahara, E; Matsuura, N; Matsuura, R; Kamizato, K; Inoue, Y; Sowa, Y; Sunami, H

  REGENERATIVE THERAPY ( Regenerative Therapy )  26   508 - 519   2024.06 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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Books 【 display / non-display 】

Books 【 display / non-display 】

• Handbook of Stem Cell Therapy

  Shimizu Y, Ntege H. E, Sunami H ( Part: Multiple Authorship ,  Adipose tissue-derived regenerative cell-based therapies: Current optimisation strategies for effective treatment in aesthetic surgery )

  Springer Nature Singapore Pte Ltd.  2022.06

Presentations 【 display / non-display 】

Presentations 【 display / non-display 】

• Transplantation Device for Regenerative Therapy Using Biodegradable Nonwoven Fabrics with Strongly Oriented

  Hiroshi Sunami, Naoko Futenma, Hitoshi Nakasone, Junko Denda, Yusuke Shimizu

  2023 Tissue Engineering and Regenerative Medicine International Society - Asia Pacific (TERMIS-AP) (Hong Kong, China)  2023.10  -  2023.10 

• The shape of a microfabricated scaffold can control cell protrusion and migration direction

  H. Sunami

  Cell Bio 2022 (Washington DC, USA)  2022.12  -  2022.12 

• Human cell resource supply system from University of the Ryukyus: the first Industries Use Ethical Committee in Japan

  清水雄介, 中村博幸, 植田真一郎, 銘苅桂子, 平田哲生, 東千夏, 池原由美, 角南寛

  2022.03  -  2022.03 

• Stem cell separation using micro-patterned scaffolds

  H. Sunami, Y. Shimizu, I. Yokota, J. Denda, Y. Igarashi

  2017 Tissue Engineering & Regenerative Medicine International Society- Asia Pacific Meeting (TERMIS-AP) (Brisbane, Australia)  2019.10  -  2019.10 

• Development of a new taxis assay using micro-patterned scaffolds

  Hiroshi SUNAMI, Yusuke SHIMIZU, Junko DENDA, Ikuko YOKOTA, Yasuyuki IGARASHI

  IVC21 (Malmö, Sweden)  2019.07  -  2019.07 

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Academic Awards 【 display / non-display 】

Academic Awards 【 display / non-display 】

• 4th TOBIRA Research Grant Encouragement Award

  2016.05   Tokyo Biomarker Innovation Research Association: TOBIRA  

Grant-in-Aid for Scientific Research 【 display / non-display 】

Grant-in-Aid for Scientific Research 【 display / non-display 】

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2024.04  -  2027.03 

  Direct: 3,500,000 (YEN)  Overheads: 4,550,000 (YEN)  Total: 1,050,000 (YEN)

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2024.04  -  2027.03 

  Direct: 3,600,000 (YEN)  Overheads: 4,680,000 (YEN)  Total: 1,080,000 (YEN)

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2024.04  -  2027.03 

  Direct: 3,600,000 (YEN)  Overheads: 4,680,000 (YEN)  Total: 1,080,000 (YEN)

• Development of a new microscopic observation method for cross-sectional observation of living cell membrane structures

  Challenging research (sprout)

  Project Year: 2022.06  -  2025.03 

  Direct: 4,900,000 (YEN)  Overheads: 6,370,000 (YEN)  Total: 1,470,000 (YEN)

• Development of new preventive method for postoperative paraplegia of thoracoabdominal aorta using exosomes

  Grant-in-Aid for Scientific Research(C)

  Project Year: 2022.04  -  2025.03 

  Direct: 3,200,000 (YEN)  Overheads: 4,160,000 (YEN)  Total: 960,000 (YEN)

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Media Coverage 【 display / non-display 】

Media Coverage 【 display / non-display 】

• 画期的なエクソソーム精製技術の開発 ～エクソソーム応用研究によるメディカルヘルスケア領域発展～

  琉球大学  プレスリリース情報  2022.12

• バイオメディカル分野の新技術を開発

  琉球大学  プレスリリース情報  2020.10

• 綿状人工骨 再生医療に オルソリバース 幹細胞、繊維構造埋め効率増殖  Newspaper, magazine

  日経産業新聞  2018.4

• 琉球大発VB グランセル 再生医療技術で化粧品 幹細胞の培養液使う  Newspaper, magazine

  日本経済新聞  2017.9

• 再生医療 産業化へ実験 琉球大 脂肪幹細胞移植に成功 大量培養し創薬目指す  Newspaper, magazine

  日本経済新聞  2016.3

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