論文 - 新里　尚也

Antifungal activity of microbes obtained from subtropical region, Okinawa, against Magnaporthe oryzae

Ueno M, Quyet NT, Shinzato N, Matsui T

Tropical agriculture and development   60 ( 1 ) 48 - 52   2016年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

Metabolic pathways in the mixed segment of the wood-feeding termite Nasutitermes takasagoensis (Blattodea (Isoptera): Termitidae)

Rohitha P. Kumara, Seikoh Saitoh, Hiroaki Aoyama, Naoya Shinzato, Gaku Tokuda

APPLIED ENTOMOLOGY AND ZOOLOGY ( SPRINGER JAPAN KK )  51 ( 3 ) 429 - 440   2016年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

Alimentary canals of higher termites are equipped with a complex structural segment consisting of mesenteric and proctodeal epithelial tissues, which is termed the mixed segment. To assess metabolic functions occurring in the mixed segment, Nasutitermes takasagoensis (Shiraki) transcriptomes were obtained from the mixed segment and adjacent gut regions (midgut and first proctodeal segment) and were annotated using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. The predominant metabolic function detected in the mixed segment was energy production via oxidative phosphorylation. As KEGG orthologs involved in lysosomal and phagosomal systems were also characteristic of this segment, cellular components are probably recycled rather than newly produced, allowing higher energy production. The predominant adenosine triphosphate (ATP) consumer in the mixed segment was vacuolar-type H+-ATPase (V-ATPase), known to be involved in K+ transport in many insects. Based on our data, V-ATPases appear to electrogenically drive NHE6 or NHE7 (NHE6/7) K+/H+ antiporters to stimulate K+ secretion, increasing gut lumen pH. The mixed segment also expressed higher levels than did the adjacent gut regions of carbonic anhydrase, another contributor to gut alkalization through HCO3 (-) production. Thus, metabolic pathways in the mixed segment might promote lignocellulose solubilization in higher termites' gut, contributing to their success.

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DNA metabarcoding of springtails (Collembola)

Seikoh Saitoh, Hiroaki Aoyama, Saori Fujii, Haruki Sunagawa, Hideki Nagahama, Masako Akutsu, Naoya Shinzato, Nobuhiro Kaneko, Taizo Nakamori

GENOME ( CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS )  58 ( 5 ) 274 - 274   2015年05月 [ 査読有り ]

掲載種別: 研究論文（国際会議プロシーディングス）

Predominant expression and activity of vacuolar H(+)-ATPases in the mixed segment of the wood-feeding termite Nasutitermes takasagoensis.

Kumara RP, Saitoh S, Aoyama H, Shinzato N, Tokuda G

Journal of insect physiology ( PERGAMON-ELSEVIER SCIENCE LTD )  78   1 - 8   2015年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

The mixed segment is a unique part of the gut present only in the most apical lineage of termites and consists of a complex of overlapping mesenteric and proctodeal epithelia. In spite of its unique structure, the physiological functions of the mixed segment have been poorly studied. We performed transcriptome analysis to identify functional enzymes acting in the mixed segment of the wood-feeding higher termite Nasutitermes takasagoensis. We sequenced the transcripts (4563 isotigs) of the mixed segment and compared them with those of the midgut (4813 isotigs) and the first proctodeal segment (3629 isotigs). We found that vacuolar H+-ATPase (V-ATPase) subunits were predominant in the mixed segment, which was confirmed by RT-qPCR analysis. The V-ATPase activity in these three tissues was in a good agreement with the expression patterns, suggesting that V-ATPase is a prevalent enzyme in the mixed segment of the termites. The results confirmed the proposed role of the mixed segment as a transporting epithelium. (C) 2015 Elsevier Ltd. All rights reserved.

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A rapid method of non-destructive DNA extraction from individual springtails (Collembola)

Hiroaki Aoyama, Seikoh Saitoh, Saori Fujii, Hideki Nagahama, Naoya Shinzato, Nobuhiro Kaneko, Taizo Nakamori

APPLIED ENTOMOLOGY AND ZOOLOGY ( SPRINGER JAPAN KK )  50 ( 3 ) 419 - 425   2015年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

In this study, we describe an easy and rapid method for non-destructive DNA extraction from a single Collembola individual, without dissection, lysis of the specimen, or purification of extracted DNA. We demonstrate that, after a single specimen has been heat-treated in Tris-EDTA (TE) buffer using a standard thermocycler, the solution can be used for PCR amplification of the mitochondrial cytochrome c oxidase subunit 1 (COI) gene region, typically used for DNA barcoding. With this method, the morphological features of Collembola commonly used for species identification are well preserved. This DNA extraction method is preferable for DNA barcoding where the sequencing and preservation of a large number of small and fragile specimens are required.

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Isolation and validation of eight microsatellite loci in a damselfly Ischnura senegalensis (Odonata: Coenagrionidae) by pyrosequencing a bead-enriched library

Takaki Kurita, Hiroaki Aoyama, Seikoh Saitoh, Naoya Shinzato, Kouji Sawada, Takashi Kuriwada, Kumiko Hironaka, Nobuyuki Inomata, Kazunori Yamahira, Mamoru Toda

Applied Entomology and Zoology ( Springer-Verlag Tokyo )  49 ( 4 ) 623 - 626   2014年08月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Development and characterization of 24 microsatellite markers in a eublepharid gecko, Goniurosaurus kuroiwae

Takaki Kurita, Hiroaki Aoyama, Seikoh Saitoh, Naoya Shinzato, Masanao Honda, Mamoru Toda

Conservation Genetics Resources   6 ( 1 ) 247 - 249   2013年10月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Degradation of oil tank sludge using long-chain alkane-degrading bacteria

Toru Matsui, Takahiro Yamamoto, Naoya Shinzato, Tsukasa Mitsuta, Kazuma Nakano, Tomoyuki Namihira

Annals of Microbiology ( BMC )  64 ( 1 ) 391 - 395   2013年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Genomic sequencing-based detection of large deletions in Rhodococcus rhodochrous strain B-276

Seikoh Saitoh, Hiroaki Aoyama, Masako Akutsu, Kazuma Nakano, Naoya Shinzato, Toru Matsui

Journal of Bioscience and Bioengineering   116 ( 3 ) 309 - 312   2013年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Antibiotics production by an actinomycete isolated from the termite gut

Toru Matsui, Junichi Tanaka, Tomoyuki Namihira, Naoya Shinzato

Journal of Basic Microbiology ( WILEY-BLACKWELL )  52 ( 6 ) 731 - 735   2012年02月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Comparative proteomic analysis of Methanothermobacter themautotrophicus ΔH in pure culture and in co-culture with a butyrate-oxidizing bacterium

Miho Enoki, Naoya Shinzato, Hiroaki Sato, Kohei Nakamura, Yoichi Kamagata

Miho Enoki, Naoya Shinzato, Hiroaki Sato, Kohei Nakamura, Yoichi Kamagata ( PLoS ONE )  6 ( 8 ) e24309   2011年08月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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The effect of nitrogen fertilization to the sward on guineagrass (Panicum maximum Jacq cv. Gatton) silage fermentation

Tomoyuki Namihira, Naoya Shinzato, Hikaru Akamine, Ichiro Nakamura, Hideaki Maekawa, Yasuhiro Kawamoto, Toru Matsui

Tomoyuki Namihira, Naoya Shinzato, Hikaru Akamine, Ichiro Nakamura, Hideaki Maekawa, Yasuhiro Kawamoto, Toru Matsui ( Asian Australasian Association of Animal Production Societies )  24 ( 3 ) 358 - 363   2011年02月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Isolation of salt stress tolerance genes from roots of mangrove plant, Rhizophora stylosa Griff., using PCR-based suppression subtractive hybridization

Mohammad Basyuni, Yuji Kinjo, Shigeyuki Baba, Naoya Shinzato, Hironori Iwasaki, Edy B. M. Siregar, Hirosuke Oku

Plant Molecular Biology Reporter ( Springer )  29   533 - 543   2010年10月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Optimized culture conditions for the efficient production of porcine adenylate kinase in recombinant Escherichia coli

Toru Matsui, Takashi Togari, Satoru Misawa, Tomoyuki Namihira, Naoya Shinzato, Hitoshi Matsuda, Seigo Sato

Applied Biochemistry and Biotechnology ( HUMANA PRESS INC )  162 ( 3 ) 823 - 829   2010年02月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Biodegradation of thiodiglycol, a hydrolyzate of the chemical weapon Yperite, by benzothiophene-desulfurizing bacteria

Leila El Bassi, Naoya Shinzato, Tomoyuki Namihira, Hirosuke Oku, Toru Matsui

Journal of Hazardous Materials ( ELSEVIER SCIENCE BV )  167 ( 1-3 ) 124 - 127   2009年08月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Biopreservation of refrigerated and vacuum-packed Dicentrarchus labrax by lactic acid bacteria

Leila El Bassi, Mnasser Hassouna, Naoya Shinzato, Toru Matsui

Journal of Food Science ( WILEY-BLACKWELL PUBLISHING, INC )  74 ( 6 ) M335 - M339   2009年07月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Stereospecific degradation of phenylsuccinate by actinomycetes

Toru Matsui, Katsuya Kato, Tomoyuki Namihira, Naoya Shinzato, Hisashi Semba

Chemosphere ( PERGAMON-ELSEVIER SCIENCE LTD )  76 ( 9 ) 1278 - 1282   2009年06月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Application of random amplified polymorphic DNA (RAPD) analysis coupled with microchip electrophoresis for high-resolution identification of Monascus strains

Naoya Shinzato, Tomoyuki Namihira, Yasutomo Tamaki, Masatoshi Tsukahara, Toru Matsui

Applied Microbiology and Biotechnology ( SPRINGER )  82 ( 6 ) 1187 - 1193   2009年03月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Shinella yambaruensis sp. nov., a 3-methyl-sulfolane-assimilating bacterium isolated from soil

Toru Matsui, Naoya Shinzato, Hideyuki Tamaki, Mizuho Muramatsu, Satoshi Hanada

International Journal of Systematic and Evolutionary Microbiology ( SOC GENERAL MICROBIOLOGY )  59 ( 3 ) 536 - 539   2009年03月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Red yeast rice fermentation by selected Monascus sp. with deep-red color, lovastatin production but no citrinin, and effect of temperature-shift cultivation on lovastatin production

Masatoshi Tsukahara, Naoya Shinzato, Yasutomo Tamaki, Tomoyuki Namihira, Toru Matsui

Applied Biochemistry and Biotechnology ( HUMANA PRESS INC )  158 ( 2 ) 476 - 482   2009年02月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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