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Published Papers 【 display / non-display 】

• Molecular cloning of putative chloroplastic cysteine synthase in Leucaena leucocephala.

  Harun-Ur-Rashid M, Oogai S, Parveen S, Inafuku M, Iwasaki H, Fukuta M, Amzad Hossain M, Oku H

  Journal of plant research ( Journal of Plant Research )  133 ( 1 ) 95 - 108   2020.01 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  Cysteine biosynthesis is directed by the successive commitments of serine acetyltransferase, and O-acetylserine (thiol) lyase (OASTL) compounds, which subsequently frame the decameric cysteine synthase complex. The isoforms of OASTL are found in three compartments of the cell: the cytosol, plastid, and mitochondria. In this investigation, we first isolated putative chloroplastic OASTL (Ch-OASTL) from Leucaena leucocephala, and the Ch-OASTL was then expressed in BL21-competent Escherichia coli. The putative Ch-OASTL cDNA clone had 1,543 base pairs with 391 amino acids in its open reading frame and a molecular weight of 41.54 kDa. The purified protein product exhibited cysteine synthesis ability, but not mimosine synthesis activity. However, they both make the common α-aminoacrylate intermediate in their first half reaction scheme with the conventional substrate O-acetyl serine (OAS). Hence, we considered putative Ch-OASTL a cysteine-specific enzyme. Kinetic studies demonstrated that the optimum pH for cysteine synthesis was 7.0, and the optimum temperature was 40 °C. In the cysteine synthesis assay, the Km and kcat values were 838 ± 26 µM and 72.83 s-1 for OAS, respectively, and 60 ± 2 µM and 2.43 s-1 for Na2S, respectively. We can infer that putative Ch-OASTL regulatory role is considered a sensor for sulfur constraint conditions, and it acts as a forerunner of various metabolic compound molecules.

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• Molecular characterization of mimosinase and cystathionine β-lyase in the Mimosoideae subfamily member Mimosa pudica.

  Oogai S, Fukuta M, Watanabe K, Inafuku M, Oku H

  Journal of plant research ( Journal of Plant Research )    2019.07

  Type of publication: Research paper (scientific journal)

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• Cytosolic Cysteine Synthase Switch Cysteine and Mimosine Production in Leucaena leucocephala.

  Md Harun-Ur-Rashid, Iwasaki H, Parveen S, Oogai S, Fukuta M, Hossain MA, Anai T, Oku H

  Applied biochemistry and biotechnology ( Applied Biochemistry and Biotechnology )  186 ( 3 ) 613 - 632   2018.11

  Type of publication: Research paper (scientific journal)

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• Molecular characterization of cytosolic cysteine synthase in Mimosa pudica.

  Rashid MH, Iwasaki H, Oogai S, Fukuta M, Parveen S, Hossain MA, Anai T, Oku H

  Journal of plant research ( Journal of Plant Research )  131 ( 2 ) 319 - 329   2018.03

  Type of publication: Research paper (scientific journal)

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• Temperature controls on the basal emission rate of isoprene in a tropical tree Ficus septica: exploring molecular regulatory mechanisms.

  Mutanda I, Inafuku M, Saitoh S, Iwasaki H, Fukuta M, Watanabe K, Oku H

  Plant, cell & environment ( Plant Cell and Environment )  39 ( 10 ) 2260 - 75   2016.10

  Type of publication: Research paper (scientific journal)

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Books 【 display / non-display 】

Books 【 display / non-display 】

• Hand book of Glycosyltransferases and Related Genes

  福田雅一 ( Part: Multiple Authorship )

  その他の出版機関  2002.03