名嘉地 めぐみ (ナカチ メグミ)

NAKACHI-MIYAGI Megumi

写真a

職名

講師

科研費研究者番号

60632015

研究室住所

〒901-0215 沖縄県中頭郡西原町上原207番地

メールアドレス

メールアドレス

研究室電話

098-895-1214

ホームページ

http://www.kyouiku.med.u-ryukyu.ac.jp/

現在の所属組織 【 表示 / 非表示

  • 専任   琉球大学   医学部   講師  

出身大学院 【 表示 / 非表示

  • 2011年04月
    -
    2015年03月

    九州大学  医学系研究科  分子常態医学専攻(臨床薬理学)  博士課程  修了

取得学位 【 表示 / 非表示

  • 九州大学 -  博士(医学)  医学

職歴 【 表示 / 非表示

  • 2011年11月
    -
    2012年12月

      琉球大学医学部附属病院 専門研修センター 特命助教  

  • 2012年12月
    -
    2016年01月

      琉球大学 医学部 医学教育企画室  特命助教  

  • 2016年02月
    -
    継続中

      琉球大学 医学部 医学教育企画室  講師  

所属学会・委員会 【 表示 / 非表示

  •  
     
     
     

    日本医学教育学会

  •  
     
     
     

    日本腎臓学会

研究キーワード 【 表示 / 非表示

  • 医学教育

  • 食育

  • 社会疫学

  • 動脈硬化

  • シェアストレス

学位論文 【 表示 / 非表示

  • Activator protein-1 mediates shear stress-induced prostaglandin d synthase gene expression in vascular endothelial cells.

    2005年05月 

    DOI PubMed

論文 【 表示 / 非表示

  • Age- and sex-related effects on ankle-brachial index in a screened cohort of Japanese: the Okinawa Peripheral Arterial Disease Study (OPADS).

    Ishida A, Miyagi M, Kinjo K, Ohya Y.

    Eur J Prev Cardiol.   21 ( 6 ) 712 - 718   2014年06月 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

     概要を見る

    AIMS: Age-related change of ankle-brachial index (ABI) within the general population, especially <40 years, has not been determined in large population studies. We evaluated the value of the ABI by age- and sex-related differences in a screened cohort. METHODS AND RESULTS: The ABI was examined in 13,211 participants (aged 21-89 years) in a health evaluation programme. The mean ABI was lower in women than in men at all ages. The ABI was lowest at <40 years, and increased with age; the maximum was at 60-69 years in both sexes. In participants <40 years, 186 (22%) of women and 108 (9.8%) of men had a borderline ABI (0.9-1.0). The prevalence of an ABI ≤0.9 in men increased with age, and sharply rose to 3.3% at ≥70 years. Conversely, women demonstrated a J-curve relationship, where the prevalence of an ABI ≤0.9 was lowest at 60-69 years (0.2%), and increased at <40 (0.9%) and ≥70 years (1.6%). The prevalence of atherosclerotic risk factors was higher in participants with an ABI ≤0.9 than those with an ABI >0.9 in men, both ≤60 and >60 years, and in women >60 years. In women ≤60 years, however, the prevalence of atherosclerotic risk factors in participants with an ABI >0.9 was as small as those with an ABI ≤0.9. CONCLUSION: The ABI increased with age until 60-69 years, and was lower in women than in men. It is unlikely that a low ABI in younger healthy women always indicate that existence of arterial stenosis.

  • Improvement of cardiac function after granulocyte-colony stimulating factor-mobilized peripheral blood mononuclear cell implantation in a patient with non-ischemic dilated cardiomyopathy associated with thromboangiitis obliterans

    Ayano Kakihana, Akio Ishida, Megumi Miyagi, Tetsuya Ishiki, Koichiro Okumura, Tomomasa Kamiyama, Yusuke Ohya, Shuichi Takishita

    Intern Med .   48 ( 12 ) 1003 - 1007   2009年06月 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

     概要を見る

    Cardiac involvement is a rare complication with thromboangiitis obliterans (TAO). We report a 29-year-old man with TAO accompanied with non-ischemic dilated cardiomyopathy. He had no history of heart disease, but echocardiogram demonstrated diffuse hypokinesis and dilated left ventricle. Coronary angiography revealed no organic stenotic lesion. For limb salvage, he was treated with granulocyte-colony stimulating factor (G-CSF)-mobilized peripheral blood mononuclear cell (PBMNC) implantation on his right leg. Not only ischemic leg symptoms, but also plasma level of BNP and (123)I-metaiodobenzylguanidine scintigraphic parameters improved after 24 weeks. G-CSF-mobilized PBMNC implantation could be an effective approach to treating non-ischemic cardiomyopathy.

  • Activator protein-1 mediates shear stress-induced prostaglandin d synthase gene expression in vascular endothelial cells.

    Miyagi M, Miwa Y, Takahashi-Yanaga F, Morimoto S, Sasaguri T.

    Arterioscler Thromb Vasc Biol.   25 ( 5 ) 970 - 975   2005年05月 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

     概要を見る

    OBJECTIVE: We attempted to determine the molecular mechanism of fluid shear stress-induced lipocalin-type prostaglandin D synthase (l-PGDS) expression in vascular endothelial cells. METHODS AND RESULTS: We examined the promoter region of the l-PGDS gene by loading laminar shear stress (20 dyne/cm2), using a parallel-plate flow chamber, on endothelial cells transfected with luciferase reporter vectors containing the 5'-flanking regions of the human l-PGDS gene. A deletion mutant analysis revealed that a shear stress-responsive element resided in the region between -2607 and -2523 bp. A mutation introduced into the putative binding site for activator protein-1 (AP-1) within this region eliminated the response to shear stress. In an electrophoretic mobility shift assay, shear stress stimulated nuclear protein binding to the AP-1 binding site, which was supershifted by antibodies to c-Fos and c-Jun. Shear stress elevated the c-Jun phosphorylation level in a time-dependent manner, similar to that of l-PGDS gene expression. SP600125, a c-Jun N-terminal kinase inhibitor, decreased the c-Jun phosphorylation, DNA binding of AP-1, and l-PGDS expression induced by shear stress. Additionally, an mRNA chase experiment using actinomycin D demonstrated that shear stress did not stabilize l-PGDS mRNA.

  • Physiology and pharmacology of the prostaglandin J2 family.

    Miwa Y, Taba Y, Miyagi M, Sasaguri T.

    Nihon Yakurigaku Zasshi   123 ( 1 ) 34 - 40   2004年01月

    掲載種別: 研究論文(学術雑誌)

     概要を見る

    The prostaglandin (PG) J(2) family including PGJ(2), Delta(12)-PGJ(2), and 15-deoxy-Delta(12,14)-PGJ(2) (15d-PGJ(2)) are metabolites of PGD(2). They had been known as powerful inhibitors of cell proliferation and viral replication until 15d-PGJ(2) was found to be a natural ligand for peroxisome proliferator-activated receptor gamma (PPAR gamma). Since then, several new pharmacological actions of the PGJ(2) family have been found, such as pro- and anti-apoptotic effects, cell differentiation-inducing effects, and inhibitory effects on inflammatory processes, whether they depend on PPAR gamma or not. We reported that the PGJ(2) family, particularly 15d-PGJ(2), inhibits cell proliferation by reducing the expression of G(1) cyclins and inducing the expression of cyclin-dependent kinase inhibitor p21 and moreover, induces cell differentiation in vascular smooth muscle cells. In vascular endothelial cells, we found that 15d-PGJ(2) inhibits apoptotic cell death at least in part by the induction of the inhibitor of apoptosis protein c-IAP1. More importantly, physiological levels of laminar fluid shear stress loaded on endothelial cells upregulate the expression of lipocalin-type PGD(2) synthase, which converts PGH(2) to PGD(2), the precursor of the PGJ(2) family. Based on these results, we have hypothesized that the PGJ(2) family synthesized in vascular wall plays an important physiological role to protect vascular cells from atherogenic stimuli.

  • 15-deoxy-delta 12,14-prostaglandin J2 and laminar fluid shear stress stabilize c-IAP1 in vascular endothelial cells.

    Taba Y, Miyagi M, Miwa Y, Inoue H, Takahashi-Yanaga F, Morimoto S, Sasaguri T.

    Am J Physiol Heart Circ Physiol.   285 ( 1 ) 38 - 46   2003年06月 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

     概要を見る

    Laminar shear stress strongly inhibits vascular endothelial cell apoptosis by unknown mechanisms. We reported that shear stress stimulates endothelial cells to produce 15-deoxy-Delta12,14-prostaglandin J2 (15d-PGJ2) by elevating the expression level of lipocalin-type prostaglandin D synthase. To investigate the role of 15d-PGJ2 produced in the vascular wall, we examined the effect of 15d-PGJ2 on endothelial cell apoptosis. We induced apoptosis in human umbilical vein endothelial cells (HUVECs) by growth factor deprivation. 15d-PGJ2 strongly inhibited DNA ladder formation, nuclear fragmentation, and caspase-3-like activity in HUVECs. To elucidate the mechanism by which 15d-PGJ2 inhibits endothelial cell apoptosis, we examined expression of the inhibitor of apoptosis proteins (IAP) cellular-IAP1 (c-IAP1), c-IAP2, x-linked IAP, and survivin in HUVECs. In parallel with the inhibition of apoptosis, 15d-PGJ2 elevated the expression level of c-IAP1 protein in a dose- and time-dependent manner without changing the mRNA level. Laminar shear stress also induced c-IAP1 expression. Chase experiments with the use of cycloheximide revealed that 15d-PGJ2 and shear stress both inhibited the proteolytic degradation of c-IAP1 protein. These results suggested that 15d-PGJ2 inhibits endothelial cell apoptosis through, at least in part, c-IAP1 protein stabilization. This mechanism might be involved in the antiapoptotic effect of laminar shear stress.

全件表示 >>

研究発表等の成果普及活動 【 表示 / 非表示

  • 琉球大学における臨床実習の取り組みと現状 ~学生アンケート結果より~

    名嘉地めぐみ、大内元、屋良さとみ、山本秀幸

    第52回日本医学教育学会大会  (鹿児島)  2020年07月  -  2020年07月   

  • 琉球大学における臨床実習の取り組み 〜学生アンケート結果より〜

    名嘉地 めぐみ

    第51回医学教育学会大会  (京都)  2019年07月  -  2019年07月   

  • 学生企画 地域医療を学ぶための学生セミナーに参加して

    上里 美也子、大庭 千穂 、名嘉地 めぐみ、武村 克哉、屋良 さとみ、奥村 耕一郎、川妻 由和、山本秀幸

    第51回日本医学教育学会大会  (京都)  2019年07月  -  2019年07月   

  • 成績不振者を対象とした個別面談による学修支援と聞き取り調査

    名嘉地 めぐみ

    第50回医学教育学会大会  2018年  -  2018年   

  • 琉球大学医学部における臨床実習改革の取り組みとその効果

    名嘉地 めぐみ

    第49回医学教育学会大会  2017年  -  2017年   

科研費獲得情報 【 表示 / 非表示

  • 潜在性鉄欠乏が血管内皮に及ぼす影響

    挑戦的研究(萌芽)

    課題番号: 25560354

    研究期間: 2013年04月  -  2016年03月 

    代表者: 宮城 めぐみ 

    直接経費: 22,000,000(円)  間接経費: 660,000(円)  金額合計: 2,860,000(円)

     概要を見る

    本研究では有経女性の体内鉄貯蔵量と血管内皮機能の関係を明らかにするために、20-40代の女性を対象に体内鉄貯蔵量の指標であるsTfR(可溶性トランスフェリン受容体)/Log血清フェリチン比、血管拡張反応検査、血管内皮前駆細胞数、酸化ストレス等を測定した。単回帰解析の結果、sTfR/Log血清フェリチン比と血管拡張反応であるFMD(%)には有意な負の影響を、HbA1c(%)は有意な正の影響を与えることが明らかとなった。したがって、有経女性において、鉄欠乏になると血管拡張反応が低下することが示唆され、鉄欠乏という栄養欠損が血管内皮に負の影響を及ぼす可能性が示唆された。