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Analysis of the upstream genetic structures of the ISEcp1-bla _CTX-M transposition units in Escherichia coli isolates carrying bla _CTX-M obtained from the Indonesian and Vietnamese communities.

Widyatama FS, Yagi N, Sarassari R, Shirakawa T, Le DT, Bui MHT, Kuntaman K, Hirai I

Microbiology and immunology     2021年09月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Comparison of species, virulence genes and clones of Aeromonas isolates from clinical specimens and well water in Okinawa Prefecture, Japan

Miyagi K.

Journal of Applied Microbiology ( Journal of Applied Microbiology )  131 ( 3 ) 1515 - 1530   2021年09月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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A high-throughput sequencing determination method for upstream genetic structure (UGS) of ISEcp1-bla_CTX-M transposition unit and application of the UGS to classification of bacterial isolates possessing bla_CTX-M.

Yagi N, Hamamoto K, Thi Bui KN, Ueda S, Tawata S, Le DT, Thi Bui MH, Hirai I

Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy ( Journal of Infection and Chemotherapy )  27 ( 9 ) 1288 - 1294   2021年09月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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INTRODUCTION: Because blaCTX-M is responsible for resistance of bacteria to the third generation cephalosporins, location of blaCTX-M could be a good indicator for classifying bacterial isolates harboring blaCTX-M in molecular epidemiology. However, determination of blaCTX-M location has been difficult when multiple copies of ISEcp1 were found on bacterial genome. We aimed to establish a high-throughput analytical method for upstream genetic structures (UGS) of ISEcp1 to facilitate determination of blaCTX-M location. METHODS: Extracted DNA samples obtained from 168 Escherichia coli isolates possessing blaCTX-M were digested by restriction enzyme, HaeIII, and the digested DNA fragments were ligated with homemade barcode adaptors. Then, DNA fragments containing UGS of ISEcp1 were amplified and subjected to the Nanopore sequencer. RESULTS: Nucleotide sequences and locations of 168 UGSs obtained from the examined E. coli isolates were determined. Among the 168 determined UGSs, 150 (89.3%) UGS were confirmed on plasmid and classified into eight types. Interestingly, coding sequence of ISEcp1 transposase gene in seven of the eight types were disrupted by IS26 insertion. The remaining 18 (10.7%) UGSs were observed in identical chromosomal region. The obtained nucleotide sequences the locations of UGSs were confirmed by conventional capillary sequencer and Southern blotting, respectively, and any discrepant result was not observed with these confirmation procedures. CONCLUSIONS: Our results indicated that the established method was efficient for simultaneously determining at least 100 different UGS, and suggested that the determined UGSs of ISEcp1-blaCTX-M transposition unit was useful for classification of bacterial isolates harboring blaCTX-M.

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Occurrence of Carriage of Multidrug Resistant Enterobacteriaceae among Pregnant Women in the Primary Health Center and Hospital Setting in Surabaya, Indonesia.

Oktaviani Sulikah SR, Hasanah M, Setyarini W, Parathon H, Kitagawa K, Nakanishi N, Nomoto R, Osawa K, Kinoshita S, Hirai I, Shirakawa T, Kuntaman K

Microbial drug resistance (Larchmont, N.Y.)     2021年08月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Objectives: The incidence of healthy individuals carrying multidrug resistant Enterobacteriaceae, including extended-spectrum β-lactamase producing Enterobacteriaceae (ESBL-E), especially extended-spectrum β-lactamase producing Escherichia coli (ESBL-EC) and extended-spectrum β-lactamase producing Klebsiella pneumoniae (ESBL-KP), is increasing worldwide. Although ESBL-E causes early or late onset of neonatal sepsis, the prevalence of ESBL-E carriage among pregnant women in Indonesia is not clear. In the present study, we compared the occurrence of carriage of ESBL-E among pregnant women in a primary health center (PHC) versus two hospitals. Materials and Methods: We collected rectal swab samples from 200 pregnant women who visited a PHC or were admitted to two hospitals in Surabaya, Indonesia from July to October 2018. The ESBL-E strains were isolated from the samples and phenotypically and genotypically analyzed. Results: ESBL-E strains were isolated from 25 (24.8%) pregnant women who visited the PHC and 49 (49.5%) pregnant women who were admitted to the hospitals. The rate of ESBL-E carriage of pregnant women in the hospitals was significantly higher than that in the PHC. Among the 74 isolated ESBL-E strains, ESBL-EC was most frequently isolated (62 strains), followed by ESBL-KP (12 strains). In addition, blaCTX-M-15 was the most frequent ESBL gene type of the isolated ESBL-E strains. Conclusions: Our results revealed the high occurrence of ESBL-E carriage in pregnant women, especially those who were admitted to the hospitals.

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Differential effects of chromosome and plasmid bla_CTX-M-15 genes on antibiotic susceptibilities in extended-spectrum beta-lactamase-producing Escherichia coli isolates from patients with urinary tract infection.

Yang YM, Osawa K, Kitagawa K, Hosoya S, Onishi R, Ishii A, Shirakawa T, Hirai I, Kuntaman K, Tanimoto H, Shigemura K, Fujisawa M

International journal of urology : official journal of the Japanese Urological Association ( International Journal of Urology )  28 ( 6 ) 623 - 628   2021年06月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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OBJECTIVES: To compare antibiotic susceptibilities between chromosomal and plasmid blaCTX-M-15 locations in urinary tract infection-causing extended-spectrum β-lactamases-producing Escherichia coli blaCTX-M-15 isolated in Indonesia. METHODS: A total of 84 strains identified as extended-spectrum β-lactamases-producing E. coli were isolated from patients with urinary tract infection in Indonesia in 2015. Antimicrobial susceptibility tests were performed on these strains using 18 antibiotics, and extended-spectrum β-lactamase bla genes were detected by polymerase chain reaction. Gene localization of blaCTX-M-15 -positive strains was confirmed by Southern blot hybridization, and epidemiological typing was conducted using multilocus sequence typing. RESULTS: Of 54 strains harboring the blaCTX-M-15 gene, 27 showed localization on chromosome, 20 on plasmid, and seven on chromosome and plasmid. Most multilocus sequence typing sequence types of the 27 strains with chromosomal blaCTX-M-15 were ST405 (25.9%) and ST131 (22.2%) strains, whereas the 20 strains with plasmid-blaCTX-M-15 were mostly ST410 (55.0%). CONCLUSIONS: Extended-spectrum β-lactamases-producing E. coli blaCTX-M-15 with plasmid genes show significantly higher resistant rates against piperacillin-tazobactam but lower resistant rates against chloramphenicol compared to chromosomal strains in Indonesian patients with urinary tract infection. Mechanistic investigations will be necessary to advance our knowledge of antimicrobial resistance in urinary tract infection.

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Observing inefficient action can induce infant preference and learning

Hirai M.

Developmental Science ( Developmental Science )    2021年 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Characterization of CTX-M-type-extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae isolated from Indonesian undergraduate medical students of a university in Surabaya, Indonesia.

Rosantia S, Higa T, Yagi N, Tokunaga T, Higa S, Yakabi Y, Shirakawa T, Kuntaman K, Hirai I

Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy ( Journal of Infection and Chemotherapy )  26 ( 6 ) 575 - 581   2020年06月 [ 査読有り ]

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High Prevalence of Colistin-Resistant Escherichia coli with Chromosomally Carried <i>mcr-1</i> in Healthy Residents in Vietnam.

Yamaguchi T, Kawahara R, Hamamoto K, Hirai I, Khong DT, Nguyen TN, Tran HT, Motooka D, Nakamura S, Yamamoto Y

mSphere   5 ( 2 )   2020年03月

掲載種別: 研究論文（学術雑誌）

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Characterization of bla_CTX-M-14 transposition from plasmid to chromosome in Escherichia coli experimental strain.

Hamamoto K, Tokunaga T, Yagi N, Hirai I

International journal of medical microbiology : IJMM ( International Journal of Medical Microbiology )  310 ( 2 ) 151395   2020年02月

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Characterisation of chromosomally-located bla_CTX-M and its surrounding sequence in CTX-M-type extended-spectrum β-lactamase-producing Escherichia coli isolates.

Hamamoto K, Hirai I

Journal of global antimicrobial resistance ( Journal of Global Antimicrobial Resistance )  17   53 - 57   2019年06月

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Characterization of CTX-M type ESBL-producing Enterobacteriaceae isolated from asymptomatic healthy individuals who live in a community of the Okinawa prefecture, Japan.

Higa S, Sarassari R, Hamamoto K, Yakabi Y, Higa K, Koja Y, Hirai I

Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy ( Journal of Infection and Chemotherapy )  25 ( 4 ) 314 - 317   2019年04月

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A survey of extended-spectrum β-lactamase-producing Enterobacteriaceae in environmental water in Okinawa Prefecture of Japan and relationship with indicator organisms.

Miyagi K, Hirai I

Environmental science and pollution research international ( Environmental Science and Pollution Research )  26 ( 8 ) 7697 - 7710   2019年03月

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Wide dissemination of colistin-resistant Escherichia coli with the mobile resistance gene mcr in healthy residents in Vietnam.

Yamamoto Y, Kawahara R, Fujiya Y, Sasaki T, Hirai I, Khong DT, Nguyen TN, Nguyen BX

The Journal of antimicrobial chemotherapy ( Journal of Antimicrobial Chemotherapy )  74 ( 2 ) 523 - 524   2019年02月

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Potential transmission opportunity of CTX-M-producing Escherichia coli on a large-scale chicken farm in Vietnam.

Bui TKN, Bui TMH, Ueda S, Le DT, Yamamoto Y, Hirai I

Journal of global antimicrobial resistance ( Journal of Global Antimicrobial Resistance )  13   1 - 6   2017年10月

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Sanitary evaluation of domestic water supply facilities with storage tanks and detection of Aeromonas, enteric and related bacteria in domestic water facilities in Okinawa Prefecture of Japan.

Miyagi K, Sano K, Hirai I

Water research ( Water Research )  119   171 - 177   2017年08月

掲載種別: 研究論文（学術雑誌）

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Common findings of bla _CTX-M-55-encoding 104-139 kbp plasmids harbored by extended-spectrum β-lactamase-producing Escherichia coli in pork meat, wholesale market workers, and patients with urinary tract infection in Vietnam.

Hoang TAV, Nguyen TNH, Ueda S, Le QP, Tran TTN, Nguyen TND, Dao TVK, Tran MT, Le TTT, Le TL, Nakayama T, Hirai I, Do TH, Vien QM, Yamamoto Y

Current microbiology   74 ( 2 ) 203 - 211   2017年02月 [ 査読有り ]

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Distribution of Aeromonas species in environmental water used in daily life in Okinawa Prefecture, Japan.

Miyagi K, Hirai I, Sano K

Environmental health and preventive medicine ( Environmental Health and Preventive Medicine )  21 ( 5 ) 287 - 294   2016年09月

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Current status of extended spectrum β-lactamase-producing Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis in Okinawa prefecture, Japan.

Nakama R, Shingaki A, Miyazato H, Higa R, Nagamoto C, Hamamoto K, Ueda S, Hachiman T, Touma Y, Miyagi K, Kawahara R, Toyosato T, Hirai I

Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy ( Journal of Infection and Chemotherapy )  22 ( 5 ) 281 - 6   2016年05月

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High Prevalence of Chromosomal blaCTX-M-14 in Escherichia coli Isolates Possessing blaCTX-M-14.

Hamamoto K, Ueda S, Toyosato T, Yamamoto Y, Hirai I

Antimicrobial agents and chemotherapy   60 ( 4 ) 2582 - 4   2016年04月

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Antibody with an engineered Fc region as a therapeutic agent against dengue virus infection.

Ramadhany R, Hirai I, Sasaki T, Ono K, Ramasoota P, Ikuta K, Kurosu T

Antiviral research   124   61 - 8   2015年12月

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