Current Affiliation Organization 【 display / non-display 】

Current Affiliation Organization 【 display / non-display 】

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Academic degree 【 display / non-display 】

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Research Interests 【 display / non-display 】

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Research Areas 【 display / non-display 】

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Thesis 【 display / non-display 】

• The role of Ca, calmodulin-dependent phosphorylation in the regulation of microtubule formation

  1985.03

Published Papers 【 display / non-display 】

Published Papers 【 display / non-display 】

• Roles of Pyk2 in signal transduction after gonadotropin-releasing hormone receptor stimulation.

  Okitsu-Sakurayama S, Higa-Nakamine S, Torihara H, Higashiyama S, Yamamoto H

  Journal of cellular physiology ( Journal of Cellular Physiology )    2020.09 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Regulation of epidermal growth factor receptor expression and morphology of lung epithelial cells by interleukin-1β.

  Nakayama I, Higa-Nakamine S, Uehara A, Sugahara K, Kakinohana M, Yamamoto H

  Journal of biochemistry ( Journal of biochemistry )  168 ( 2 ) 113 - 123   2020.08 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Fyn-mediated phosphorylation of Pyk2 promotes its activation and dissociation downstream of gonadotropin-releasing hormone receptor.

  Higa-Nakamine S, Okitsu-Sakurayama S, Kina S, Yamamoto H

  The FEBS journal ( FEBS Journal )  287 ( 16 ) 3551 - 3564   2020.08 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Increased expression of EGR1 and KLF4 by polysulfide via activation of the ERK1/2 and ERK5 pathways in cultured intestinal epithelial cells.

  Arakaki K, Uehara A, Higa-Nakamine S, Kakinohana M, Yamamoto H

  Biomedical research (Tokyo, Japan) ( バイオメディカルリサーチプレス )  41 ( 3 ) 119 - 129   2020 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  <p>Sodium trisulfide (Na₂S₃) releases hydrogen polysulfide (H₂S_n) and is useful for the investigation of the effects of H₂S_n on the cell functions. In the present study, we first examined the effects of Na₂S₃ on the gene expression of IEC-6 cells, a rat intestinal epithelial cell line. Microarray analysis and reverse transcription-polymerase chain reaction analysis revealed that Na₂S₃ increased the gene expression of early growth response 1 (EGR1) and Kruppel-like transcription factor 4 (KLF4). It was interesting that U0126, an inhibitor of the activation of extracellular signal-regulated kinase 1 (ERK1), ERK2, and ERK5, inhibited the Na₂S₃-induced gene expression of EGR1 and KLF4. Na₂S₃ activated ERK1 and ERK2 (ERK1/2) within 15 min. In addition to ERK1/2, Na₂S₃ activated ERK5. We noticed that the electrophoretic mobility of ERK5 was decreased after Na₂S₃ treatment. Phos-tag analysis and <i>in vitro</i> dephosphorylation of the cell extracts indicated that the gel-shift of ERK5 was due to its phosphorylation. The gel-shift of ERK5 was inhibited completely by both U0126 and ERK5-IN-1, a specific inhibitor of ERK5. From these results, we concluded that the gel-shift of ERK5 was induced through autophosphorylation by activated ERK5 after Na₂S₃ treatment. The present study suggested that H₂S_n affected various functions of intestinal epithelial cells through the activation of the ERK1/2 and ERK5 pathways.</p>

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• Activation of Pyk2 by CaM kinase II in cultured hypothalamic neurons and gonadotroph cells.

  Okitsu-Sakurayama S, Higa-Nakamine S, Torihara H, Takahashi H, Higashiyama S, Yamamoto H

  Journal of cellular physiology ( Journal of Cellular Physiology )  234 ( 5 ) 6865 - 6875   2019.05 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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Grant-in-Aid for Scientific Research 【 display / non-display 】

Grant-in-Aid for Scientific Research 【 display / non-display 】

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2016.04  -  2019.03 

  Direct: 3,800,000 (YEN)  Overheads: 1,140,000 (YEN)  Total: 4,940,000 (YEN)

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2011.04  -  2013.03 

  Direct: 4,000,000 (YEN)  Overheads: 1,200,000 (YEN)  Total: 5,200,000 (YEN)

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2008  -  2010 

  Direct: 1,300,000 (YEN)  Overheads: 390,000 (YEN)  Total: 1,690,000 (YEN)