Published Papers - Toma Claudia

ELISA for leptospiral 3-hycroxyacyl-CoA-dehydrogenase in urine is a promising screening tool for acute leptospirosis

Karunadasa AKUI, Toma C*, Senaratne P, Kumara KGRA, Herath TM, Pathirage MMK, Gamage CD*

Access Microbiology   6 ( 5 ) 000651.v4   2024.05 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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Degradation of p0071 and p120-catenin during adherens junction disassembly by Leptospira interrogans

Romina Tokumon, Isabel Sebastian, Bruno M. Humble, Nobuhiko Okura, Hidenori Yamanaka, Tetsu Yamashiro, Claudia Toma

Frontiers in cellular and infection microbiology ( Frontiers )  13   2023.09 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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Analysis of human clinical and environmental Leptospira to elucidate the eco-epidemiology of leptospirosis in Yaeyama, subtropical Japan.

Sato Y, Hermawan I, Kakita T, Okano S, Imai H, Nagai H, Kimura R, Yamashiro T, Kajita T, Toma C

PLoS neglected tropical diseases ( PLoS Neglected Tropical Diseases )  16 ( 3 ) e0010234 - e0010234   2022.03 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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Background Leptospirosis, a zoonosis caused by species in the spirochete genus Leptospira, is endemic to the Yaeyama region in Okinawa, subtropical Japan. Species of the P1 subclade “virulent” group, within the genus Leptospira, are the main etiological agents of leptospirosis in Okinawa. However, their environmental persistence is poorly understood. This study used a combination of bacterial isolation and environmental DNA (eDNA) metabarcoding methods to understand the eco-epidemiology of leptospirosis in this endemic region. Findings Polymerase chain reaction (PCR) characterized twelve human clinical L. interrogans isolates belonging to the P1 subclade “virulent” subgroup and 11 environmental soil isolates of the P1subclade “low virulent” subgroup (genetically related to L. kmetyi, n = 1; L. alstonii, n = 4; L. barantonii, n = 6) from the Yaeyama region targeting four virulence-related genes (lipL32, ligA, ligB and lpxD1). Clinical isolates were PCR positive for at least three targeted genes, while all environmental isolates were positive only for lipL32. Analysis of infected renal epithelial cells with selected clinical and environmental strains, revealed the disassembly of cell-cell junctions for the Hebdomadis clinical strain serogroup. Comparison of leptospiral eDNA during winter and summer identified operational taxonomic units corresponding to the species isolated from soil samples (L. kmetyi and L. barantonii) and additional P2 subclade species (L. licerasiae, L. wolffii-related, among others) that were not detected by soil cultivation. Total Leptospira read counts were higher in summer than in winter and the analysis of leptospiral/animal eDNA relationship suggested Rattus spp. as a potential reservoir animal. Conclusion Our study demonstrated high environmental Leptospira diversity in the Yaeyama region, particularly during summer, when most of the leptospirosis cases are reported. In addition, several Leptospira species with pathogenic potential were identified that have not yet been reported in Yaeyama; however, the environmental persistence of P1 subclade species previously isolated from human clinical cases in this region was absent, suggesting the need of further methodology development and surveillance.

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Disassembly of the apical junctional complex during the transmigration of Leptospira interrogans across polarized renal proximal tubule epithelial cells.

Sebastián I, Okura N, Humbel BM, Xu J, Hermawan I, Matsuura C, Hall M, Takayama C, Yamashiro T, Nakamura S, Toma C

Cellular microbiology ( Cellular Microbiology )  23 ( 9 ) e13343   2021.09 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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Understanding leptospirosis eco-epidemiology by environmental DNA metabarcoding of irrigation water from two agro-ecological regions of Sri Lanka

Chandika D. Gamage, Yukuto Sato, Ryosuke Kimura, Tetsu Yamashiro, Claudia Toma

PLOS Neglected Tropical Diseases   14 ( 7 ) e0008437   2020.07 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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Evaluation of Intracellular Trafficking in Macrophages.

Toma C, Suzuki T

Methods in molecular biology (Clifton, N.J.) ( Methods in Molecular Biology )  2134   199 - 206   2020 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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Environmental DNA metabarcoding to detect pathogenic Leptospira and associated organisms in leptospirosis-endemic areas of Japan.

Sato Y, Mizuyama M, Sato M, Minamoto T, Kimura R, Toma C

Scientific reports ( Springer Science and Business Media LLC )  9 ( 1 ) 6575   2019.04 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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Characterizing interactions of Leptospira interrogans with proximal renal tubule epithelial cells.

Yamaguchi T, Higa N, Okura N, Matsumoto A, Hermawan I, Yamashiro T, Suzuki T, Toma C

BMC microbiology   18 ( 1 ) 64   2018.07

Type of publication: Research paper (scientific journal)

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Effects of Psidium guajava leaf extract on secretion systems of Gram-negative enteropathogenic bacteria.

Nakasone N, Ogura Y, Higa N, Toma C, Koizumi Y, Takaesu G, Suzuki T, Yamashiro T

Microbiology and immunology     2018.05

Type of publication: Research paper (scientific journal)

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Leptospiral 3-hydroxyacyl-CoA dehydrogenase as an early urinary biomarker of leptospirosis.

Toma C, Koizumi N, Kakita T, Yamaguchi T, Hermawan I, Higa N, Yamashiro T

Heliyon   4 ( 4 ) e00616   2018.04

Type of publication: Research paper (scientific journal)

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Epigallocatechin gallate inhibits the type III secretion system of Gram-negative enteropathogenic bacteria under model conditions.

Nakasone N, Higa N, Toma C, Ogura Y, Suzuki T, Yamashiro T

FEMS microbiology letters   364 ( 13 )   2017.07

Type of publication: Research paper (scientific journal)

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Complete Genome Sequences of Low-Passage Virulent and High-Passage Avirulent Variants of Pathogenic Leptospira interrogans Serovar Manilae Strain UP-MMC-NIID, Originally Isolated from a Patient with Severe Leptospirosis, Determined Using PacBio Single-Molecule Real-Time Technology.

Satou K, Shimoji M, Tamotsu H, Juan A, Ashimine N, Shinzato M, Toma C, Nohara T, Shiroma A, Nakano K, Teruya K, Terabayashi Y, Ohki S, Koizumi N, Okano S, Suzuki T, Hirano T

Genome announcements   3 ( 4 )   2015.08

Type of publication: Research paper (scientific journal)

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Leptospiral outer membrane protein LMB216 is involved in enhancement of phagocytic uptake by macrophages.

Toma C, Murray GL, Nohara T, Mizuyama M, Koizumi N, Adler B, Suzuki T

Cellular microbiology   16 ( 9 ) 1366 - 77   2014.09 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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High-temperature protein G is an essential virulence factor of Leptospira interrogans.

King AM, Pretre G, Bartpho T, Sermswan RW, Toma C, Suzuki T, Eshghi A, Picardeau M, Adler B, Murray GL

Infection and immunity   82 ( 3 ) 1123 - 31   2014.03 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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Lose the battle to win the war: bacterial strategies for evading host inflammasome activation.

Higa N, Toma C, Nohara T, Nakasone N, Takaesu G, Suzuki T.

Trends Microbiol ( その他の出版社 )  21   342 - 349   2013

Type of publication: Research paper (other science council materials etc.)

Vibrio parahaemolyticus effector proteins suppress inflammasome activation by interfering with host autophagy signaling.

Higa N, Toma C, Koizumi Y, Nohara T, Masumoto J, Kodama T, Iida T, Suzuki T.

PLOS Pathog ( その他の出版社 )  9   e1003142   2013

Type of publication: Research paper (other science council materials etc.)

Inflammasome activation via intracellular NLRs triggered by bacterial infection.

Koizumi Y, Toma C, Higa N, Nohara T, Nakasone N, Suzuki T.

Cell Microbiol. ( その他の出版社 )  14   149 - 154   2012

Type of publication: Research paper (other science council materials etc.)

Characteristics features of intracellular pathogenic Leptospira in infected murine macrophages.

Toma C, Okura N, Takayama C, Suzuki T.

Cell Microbiol. ( その他の出版社 )  13   1783 - 1792   2011

Type of publication: Research paper (other science council materials etc.)

Detergents enhance EspB secretion from Escherichia coli strains harboring the locus for the enterocyte effacement (LEE) gene.

Nakasone N, Toma C, Higa N, Koizumi Y, Ogura Y, and Suzuki T.

FEMS Microbiol. Lett. ( その他の出版社 )  315   109 - 114   2011

Type of publication: Research paper (other science council materials etc.)

Pathogenic Vibrio activate NLRP3 inflammasome via cytotoxins and TLR/nucleotide-binding oligomerization domain-mediated NF-kappa B signaling.

Toma C, Higa N, Koizumi Y, Nakasone N, Ogura Y, McCoy AJ, Franchi L, Uematsu S, Sagara J, Taniguchi S, Tsutsui H, Akira S, Tschopp J, Núñez G, Suzuki T

Journal of immunology (Baltimore, Md. : 1950)   184 ( 9 ) 5287 - 97   2010.05 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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