論文 - 水谷　治

The effect of Aspergillus luchuensis pectin methylesterase genes pmeA and pmeB on methanol production in sweet potato shochu.

Mizutani O, Kakizono D, Takahashi T, Mukai N, Fukuda H, Yamada O

Bioscience, biotechnology, and biochemistry ( Bioscience, Biotechnology and Biochemistry )  87 ( 7 ) 777 - 785   2023年06月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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全ゲノム情報を用いた黒麹菌Aspergillus luchuensisの系統解析—Phylogenetic analysis of Aspergillus luchuensis strains based on whole genome analysis

塚原 正俊, 東 春奈, 水谷 治, 山田 修, 外山 博英

日本醸造協会誌 / 日本醸造協会, 日本醸造学会 [編]   117 ( 6 ) 413 - 421   2022年06月 [ 査読有り ]

掲載種別: 研究論文（その他学術会議資料等）

Preparation and Crystal Structure Analysis of High-Strength Film Derived from Nigeran Ester Derivatives

Togo Azusa, Uechi Keiko, Mizutani Osamu, Kimura Satoshi, Iwata Tadahisa

Journal of Fiber Science and Technology ( 一般社団法人 繊維学会 )  78 ( 8 ) 126 - 132   2022年 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

<p>Nigeran (<i>α</i>-1,3-<i>alt</i>-<i>α</i>-1,4-glucan) is a linear glucan with alternating <i>α</i>-1,3- and <i>α</i>-1,4-glycosidic linkages. It is extracted from the cell walls of certain species in the genera <i>Aspergillus</i> and <i>Penicillium</i>. Nigeran can be esterified and used as a film, but its strength is only approximately 4–25 MPa. However, in the present study, high-strength nigeran ester films with tensile strengths of 100 MPa were successfully prepared by thermally stretching and annealing the melt-quenched films. Two-dimensional wide-angle X-ray diffraction (2D-WAXD) analysis revealed that the highly oriented films of nigeran butyrate (NGBu), nigeran valerate (NGVa), and nigeran hexanoate (NGHx) studied herein had two-fold helix symmetry along the molecular axis. Assuming the crystal systems to be orthorhombic, the unit lattice of each ester was calculated as NGBu (<i>a</i>=28.6Å, <i>b</i>=9.07Å, <i>c</i>=16.5Å), NGVa (<i>a</i>=31.5Å, <i>b</i>=9.07Å, <i>c</i>=16.2Å), and NGHx (<i>a</i>=36.7Å, <i>b</i>=9.07Å, <i>c</i>=16.2Å). In the calculated unit lattice parameters, only the <i>a</i>-axis was extended as the number of ester carbons increased.</p>

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Identification of Genes Involved in the Synthesis of the Fungal Cell Wall Component Nigeran and Regulation of Its Polymerization in Aspergillus <i>luchuensis</i>.

Uechi K, Yaguchi H, Tokashiki J, Taira T, Mizutani O

Applied and environmental microbiology   87 ( 21 ) e0114421   2021年10月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

Certain Aspergillus and Penicillium spp. produce the fungal cell wall component nigeran, an unbranched D-glucan with alternating α-1,3- and α-1,4-glucoside linkages, under nitrogen starvation. The mechanism underlying nigeran biosynthesis and the physiological role of nigeran in fungal survival are not clear. We used RNA-seq to identify genes involved in nigeran synthesis in the filamentous fungus Aspergillus luchuensis when grown under nitrogen-free conditions. agsB, which encodes a putative α-1,3-glucan synthase, and two adjacent genes (agtC and gnsA) were upregulated under conditions of nitrogen starvation. Disruption of agsB in A. luchuensis (ΔagsB) resulted in the complete loss of nigeran synthesis. Furthermore, overexpression of agsB in an Aspergillus oryzae strain that cannot produce nigeran resulted in nigeran synthesis. These results indicated that agsB encodes a nigeran synthase. Therefore, we have renamed the A. luchuensis agsB as nigeran synthase gene (nisA). Nigeran synthesis in an agtC mutant (ΔagtC) increased to 121%; conversely, that in ΔgnsA and ΔagtCgnsA decreased to 64% and 63%, respectively, compared to that in the wild-type strain. Our results revealed that AgtC and GnsA play an important role in regulating not only the quantity of nigeran but also its polymerization. Collectively, our results demonstrated that nisA (agsB) is essential for nigeran synthesis in A. luchuensis, whereas agtC and gnsA contribute to the regulation of nigeran synthesis and its polymerization. This research provides insights into fungal cell wall biosynthesis, specifically the molecular evolution of fungal α-glucan synthase genes and the potential utilization of nigeran as a novel biopolymer. Importance The fungal cell wall is composed mainly of polysaccharides. Under nitrogen-free conditions, some Aspergillus and Penicillium spp. produce significant levels of nigeran, a fungal cell wall polysaccharide composed of alternating α-1,3-/1,4-glucosidic linkages. The mechanisms regulating the biosynthesis and function of nigeran are unknown. Here, we performed RNA sequencing of Aspergillus luchuensis cultured under nitrogen-free or low-nitrogen conditions. A putative α-1,3-glucan synthase gene, whose transcriptional level was upregulated under nitrogen-free conditions, was demonstrated to encode nigeran synthase. Furthermore, two genes encoding an α-glucanotransferase and a hypothetical protein were shown to be involved in controlling nigeran content and molecular weight. This study reveals genes involved in the synthesis of nigeran, a potential biopolymer, and provides a deeper understanding of fungal cell wall biosynthesis.

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Expression profiles of amylolytic genes in AmyR and CreA transcription factor deletion mutants of the black koji mold Aspergillus luchuensis.

Wataru Hashimoto, Hiraku Arai, Osamu Mizutani, Osamu Yamada, Takahiro Shintani, Katsuya Gomi

Journal of bioscience and bioengineering   132 ( 4 ) 321 - 326   2021年06月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Development of an itraconazole resistance gene as a dominant selectable marker for transformation in Aspergillus oryzae and Aspergillus luchuensis.

Tokashiki J, Toyama H, Mizutani O

Bioscience, biotechnology, and biochemistry   85 ( 3 ) 722 - 727   2021年02月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Synthesis and characterization of α-1,3-alt-α-1,4-glucan (nigeran) ester derivatives

Togo A., Uechi K., Mizutani O., Kimura S., Iwata T.

Polymer ( Polymer )  214   2021年02月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Construction of transcription factor gene deletion library of <i>Aspergillus luchuensis</i>

Yamada Osamu, Nishibori Naoko, Hayashi Risa, Arima Toshihide, Mizutani Osamu

The Journal of General and Applied Microbiology ( 公益財団法人　応用微生物学・分子細胞生物学研究奨励会 )  67 ( 3 ) 118 - 123   2021年 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

<p>We selected 96 genes of <i>Aspergillus luchuensis</i> for the construction of a transcription factor gene deletion library. Of these, we successfully deleted 93 genes using <i>Agrobacterium</i>-mediated transformation (AMT) of <i>A. luchuensis</i> RIB 2604 ΔligD strains. We obtained only heterokaryonic strains after deletions of <i>adaB</i>, <i>anBH1</i>, <i>hacA</i>, <i>hapB</i>, <i>hsf1</i>, <i>metR</i>, and <i>sonC</i> gene, and additionally, could not obtain deletion strains for genes <i>abaA</i> and <i>mcmA</i>. The deletion strains will be available through our website (https://www.nrib.go.jp).</p>

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Downregulation of the ypdA Gene Encoding an Intermediate of His-Asp Phosphorelay Signaling in Aspergillus nidulans Induces the Same Cellular Effects as the Phenylpyrrole Fungicide Fludioxonil

Akira Yoshimi, Daisuke Hagiwara, Miyako Ono, Yasuyuki Fukuma, Yura Midorikawa, Kentaro Furukawa, Tomonori Fujioka, Osamu Mizutani, Natsuko Sato, Ken Miyazawa, Jun-ichi Maruyama, Junichiro Marui, Youhei Yamagata, Tasuku Nakajima, Chihiro Tanaka, Keietsu Abe

Frontiers in Fungal Biology ( Frontiers Media SA )  2   675459   2021年 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

Many eukaryotic histidine-to-aspartate (His-Asp) phosphorelay systems consist of three types of signal transducers: a His-kinase (HK), a response regulator (RR), and a histidine-containing phosphotransfer intermediate (HPt). In general, the HPt acts as an intermediate between the HK and the RR and is indispensable for inducing appropriate responses to environmental stresses. In a previous study, we attempted but were unable to obtain deletion mutants of the <italic>ypdA</italic> gene in order to characterize its function in the filamentous fungus <italic>Aspergillus nidulans</italic>. In the present study, we constructed the C<italic>ypdA</italic> strain in which <italic>ypdA</italic> expression is conditionally regulated by the <italic>A. nidulans alcA</italic> promoter. We constructed C<italic>ypdA</italic> strains with RR gene disruptions (C<italic>ypdA-sskA</italic>Δ, C<italic>ypdA-srrA</italic>Δ, and C<italic>ypdA-sskA</italic>Δ<italic>srrA</italic>Δ). Suppression of YpdA induced by <italic>ypdA</italic> downregulation activated the downstream HogA mitogen-activated protein kinase cascade. YpdA suppression caused severe growth defects and abnormal hyphae, with features such as enhanced septation, a decrease in number of nuclei, nuclear fragmentation, and hypertrophy of vacuoles, both regulated in an SskA–dependent manner. Fludioxonil treatment caused the same cellular responses as <italic>ypdA</italic> suppression. The growth-inhibitory effects of fludioxonil and the lethality caused by <italic>ypdA</italic> downregulation may be caused by the same or similar mechanisms and to be dependent on both the SskA and SrrA pathways.

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Phenolic acid decarboxylase of Aspergillus luchuensis plays a crucial role in 4-vinylguaiacol production during awamori brewing.

Maeda M, Motosoko M, Tokashiki T, Tokashiki J, Mizutani O, Uechi K, Goto M, Taira T

Journal of bioscience and bioengineering ( Journal of Bioscience and Bioengineering )  130 ( 4 ) 352 - 359   2020年10月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Aspergillus luchuensis fatty acid oxygenase ppoC is necessary for 1-octen-3-ol biosynthesis in rice koji.

Kataoka R, Watanabe T, Yano S, Mizutani O, Yamada O, Kasumi T, Ogihara J

Journal of bioscience and bioengineering ( Journal of Bioscience and Bioengineering )  129 ( 2 ) 192 - 198   2020年02月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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An updated data portal for fungal allergens with curated information.

Onami JI, Kobayashi N, Watanabe M, Yamada O, Mizutani O, Yokoyama K, Haruo T, Chibana H, Kamata Y

Bioinformation   15 ( 11 ) 820 - 823   2019年12月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Effect of pepA deletion and overexpression in Aspergillus luchuensis on sweet potato shochu brewing.

Setoguchi S, Mizutani O, Yamada O, Futagami T, Iwai K, Takase Y, Tamaki H

Journal of bioscience and bioengineering ( Journal of Bioscience and Bioengineering )  128 ( 4 ) 456 - 462   2019年10月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Influence of α-1,3-glucan synthase gene agsE on protoplast formation for transformation of Aspergillus luchuensis.

Tokashiki J, Hayashi R, Yano S, Watanabe T, Yamada O, Toyama H, Mizutani O

Journal of bioscience and bioengineering ( Journal of Bioscience and Bioengineering )  128 ( 2 ) 129 - 134   2019年08月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Yeast strains from coconut toddy in Sri Lanka show high tolerance to inhibitors derived from the hydrolysis of lignocellulosic materials

Ahangangoda Arachchige M., Osamu Mizutani, Hirohide Toyama

Biotechnology and Biotechnological Equipment ( Biotechnology and Biotechnological Equipment )  33 ( 1 ) 1505 - 1515   2019年01月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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甘藷焼酎醸造工程中の葉酸含量の推移

向井伸彦, 森本朋子, 福家成美, 上佐古大貴, 水谷　治, 西堀奈穂子, 金井宗良, 山田　修, 藤井　力

日本醸造協会誌   113 ( 2 ) 115 - 122   2018年02月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

Self-excising Cre/mutant lox marker recycling system for multiple gene integrations and consecutive gene deletions in Aspergillus oryzae.

Zhang S, Ban A, Ebara N, Mizutani O, Tanaka M, Shintani T, Gomi K

Journal of bioscience and bioengineering   123 ( 4 ) 403 - 411   2017年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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泡盛及び甘藷焼酎の揮発性成分間の相関解析

福田　央, 韓　錦順, 水谷　治, 金井宗良, 山田　修

日本醸造協会誌   112 ( 4 ) 273 - 314   2017年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

Detailed analysis of targeted gene mutations caused by the Platinum-Fungal TALENs in Aspergillus oryzae RIB40 strain and a ligD disruptant.

Mizutani O, Arazoe T, Toshida K, Hayashi R, Ohsato S, Sakuma T, Yamamoto T, Kuwata S, Yamada O

Journal of bioscience and bioengineering   123 ( 3 ) 287 - 293   2017年03月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Cellular responses to the expression of unstable secretory proteins in the filamentous fungus Aspergillus oryzae.

Yokota JI, Shiro D, Tanaka M, Onozaki Y, Mizutani O, Kakizono D, Ichinose S, Shintani T, Gomi K, Shintani T

Applied microbiology and biotechnology   101 ( 6 ) 2437 - 2446   2017年03月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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