水口 真理子 (ミズグチ マリコ)

MIZUGUCHI Mariko

写真a

科研費研究者番号

40581541

職歴 【 表示 / 非表示

  • 2012年04月
    -
    2018年01月

      東京医科歯科大学  

  • 2018年02月
    -
    2020年03月

      琉球大学  

  • 2020年04月
    -
    継続中

      琉球大学  

所属学会・委員会 【 表示 / 非表示

  •  
     
     
     

    日本癌学会

  •  
     
     
     

    日本HTLV-1学会

研究キーワード 【 表示 / 非表示

  • 成人T細胞白血病ウイルス1型

  • 成人T細胞白血病

研究分野 【 表示 / 非表示

  • ライフサイエンス / 分子生物学

論文 【 表示 / 非表示

  • Acute type adult T-cell leukemia cells proliferate in the lymph nodes rather than in peripheral blood.

    Mizuguchi M, Takatori M, Sakihama S, Yoshita-Takahashi M, Imaizumi N, Takahashi Y, Hasegawa H, Karube K, Fukushima T, Nakamura M, Tanaka Y

    Cancer gene therapy ( Cancer Gene Therapy )    2022年04月 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

  • Elevation of the Plasma Levels of TNF Receptor 2 in Association with Those of CD25, OX40, and IL-10 and HTLV-1 Proviral Load in Acute Adult T-Cell Leukemia.

    Kato M, Imaizumi N, Tanaka R, Mizuguchi M, Hayashi M, Miyagi T, Uchihara J, Ohshiro K, Todoroki J, Karube K, Masuzaki H, Tanaka Y, Fukushima T

    Viruses ( Viruses )  14 ( 4 ) 751 - 751   2022年04月 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

     概要を見る

    Adult T-cell leukemia/lymphoma (ATL) cells express TNF receptor type-2 (TNFR2) on their surface and shed its soluble form (sTNFR2). We previously reported that sTNFR2 levels were highly elevated in the plasma of patients with acute ATL. To investigate whether its quantitation would be helpful for the diagnosis or prediction of the onset of acute ATL, we examined the plasma levels of sTNFR2 in a large number of specimens obtained from a cohort of ATL patients and asymptomatic human T-cell leukemia virus type 1 (HTLV-1) carriers (ACs) and compared them to those of other candidate ATL biomarkers (sCD25, sOX40, and IL-10) by enzyme-linked immunosorbent assays (ELISA) and HTLV-1 proviral loads. We observed that sTNFR2 levels were significantly elevated in acute ATL patients compared to ACs and patients with other types of ATL (chronic, smoldering, and lymphoma). Importantly, sTNFR2 levels were significantly correlated with those of sCD25, sOX40, and IL-10, as well as proviral loads. Thus, the present study confirmed that an increase in plasma sTNFR2 levels is a biomarker for the diagnosis of acute ATL. Examination of plasma sTNFR2 alone or in combination with other ATL biomarkers may be helpful for the diagnosis of acute ATL.

  • A protective role of HTLV-1 gp46-specific neutralizing and antibody-dependent cellular cytotoxicity-inducing antibodies in progression to adult T-cell leukemia (ATL).

    Tanaka Y, Tanaka R, Imaizumi N, Mizuguchi M, Takahashi Y, Hayashi M, Miyagi T, Uchihara J, Ohshiro K, Masuzaki H, Fukushima T

    Frontiers in immunology ( Frontiers in Immunology )  13   921606   2022年 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

     概要を見る

    Human T-cell leukemia virus type-1 (HTLV-1) establishes a long-term persistent infection in humans and causes malignant T-cell leukemia, adult T-cell leukemia (ATL). HTLV-1-specific cytotoxic T lymphocytes have been suggested to play a major role in the immunosurveillance of HTLV-1-infected T cells. However, it remains unclear whether HTLV-1-specific functional antibodies are also involved in the host defense. To explore the role of antibodies in the course of HTLV-1 infection, we quantitated HTLV-1-specific neutralizing and antibody-dependent cellular cytotoxicity (ADCC)-inducing antibody levels in plasma from asymptomatic carriers (ACs) and ATL patients. The levels of neutralizing antibodies, as determined by a syncytium inhibition assay, were significantly lower in acute and chronic ATL patients than in ACs. The levels of ADCC-inducing activity were tested using an autologous pair of HTLV-1-producing cells and cultured natural killer (NK) cells, which showed that the ADCC-inducing activity of IgG at a concentration of 100 µg/ml was comparable between ACs and acute ATL patients. The anti-gp46 antibody IgG levels, determined by ELISA, correlated with those of the neutralizing and ADCC-inducing antibodies. In contrast, the proviral loads did not correlate with any of these antibody levels. NK cells and a monoclonal anti-gp46 antibody reduced the number of HTLV-1 Tax-expressing cells in cultured peripheral blood mononuclear cells from patients with aggressive ATL. These results suggest a protective role for HTLV-1 neutralizing and ADCC-inducing antibodies during the course of HTLV-1 infection.

  • Promoter CpG methylation inhibits Krüppel-like factor 2 (KLF2)-Mediated repression of hTERT gene expression in human T-cells.

    Mizuguchi M, Hara T, Yoshita-Takahashi M, Kohda T, Tanaka Y, Nakamura M

    Biochemistry and biophysics reports ( Biochemistry and Biophysics Reports )  26   100984 - 100984   2021年07月 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

  • ER-resident sensor PERK is essential for mitochondrial thermogenesis in brown adipose tissue.

    Kato H, Okabe K, Miyake M, Hattori K, Fukaya T, Tanimoto K, Beini S, Mizuguchi M, Torii S, Arakawa S, Ono M, Saito Y, Sugiyama T, Funatsu T, Sato K, Shimizu S, Oyadomari S, Ichijo H, Kadowaki H, Nishitoh H

    Life science alliance ( Life Science Alliance )  3 ( 3 )   2020年03月 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

     概要を見る

    Mitochondria play a central role in the function of brown adipocytes (BAs). Although mitochondrial biogenesis, which is indispensable for thermogenesis, is regulated by coordination between nuclear DNA transcription and mitochondrial DNA transcription, the molecular mechanisms of mitochondrial development during BA differentiation are largely unknown. Here, we show the importance of the ER-resident sensor PKR-like ER kinase (PERK) in the mitochondrial thermogenesis of brown adipose tissue. During BA differentiation, PERK is physiologically phosphorylated independently of the ER stress. This PERK phosphorylation induces transcriptional activation by GA-binding protein transcription factor α subunit (GABPα), which is required for mitochondrial inner membrane protein biogenesis, and this novel role of PERK is involved in maintaining the body temperatures of mice during cold exposure. Our findings demonstrate that mitochondrial development regulated by the PERK-GABPα axis is indispensable for thermogenesis in brown adipose tissue.

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科研費獲得情報 【 表示 / 非表示

  • ATLにおける制御性T細胞の選択的な増殖機構:OX40L/OX40に着目して

    基盤研究(C)

    課題番号: 19K08843

    研究期間: 2019年04月  -  2022年03月 

    代表者: 水口 真理子 

    直接経費: 3,400,000(円)  間接経費: 1,020,000(円)  金額合計: 4,420,000(円)

  • DNA複製異常による成人T細胞白血病発症機構の解明

    若手研究(B)

    課題番号: 17K14988

    研究期間: 2017年04月  -  2019年03月 

    代表者: 水口 真理子 

    直接経費: 2,800,000(円)  間接経費: 840,000(円)  金額合計: 3,640,000(円)