谷口 委代 (タニグチ トモヨ)

Taniguchi Tomoyo

写真a

取得学位 【 表示 / 非表示

  • 琉球大学 -  博士(医学)  ライフサイエンス / 寄生虫学

職歴 【 表示 / 非表示

  • 2009年04月
    -
    2011年03月

      日本学術振興会特別研究員(PD)  

  • 2011年07月
    -
    2019年08月

      群馬大学  

  • 2019年09月
    -
    2020年03月

      奈良県立医科大学  

  • 2020年04月
    -
    2022年03月

      日本学術振興会特別研究員(RPD)  

  • 2022年04月
    -
    継続中

      琉球大学  

論文 【 表示 / 非表示

  • Effect of α-Tocopheryloxy Acetic Acid on the Infection of Mice with Plasmodium berghei ANKA In Vivo and Humans with P. falciparum In Vitro.

    Nanang R Ariefta, Aiko Kume, Yoshifumi Nishikawa, Tomoyo Taniguchi, Rika Umemiya-Shirafuji, Shunji Kasai, Hiroshi Suzuki

    Acta parasitologica     2022年08月 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

     概要を見る

    PURPOSE: Malarial parasites are susceptible to oxidative stress. The effects of α-tocopheryloxy acetic acid (α-TEA), a vitamin E analog, on infection by Plasmodium berghei ANKA and P. falciparum in mice and human red blood cells (RBCs), respectively, were examined in this study. METHODS: For in vivo studies in mice, RBCs infected with P. berghei ANKA were inoculated via intraperitoneal injection and α-TEA was administered to C57BL/6 J male mice after infection. The blood-brain barrier (BBB) permeability was examined by Evans blue staining in experimental cerebral malaria at 7 days after infection. The in vitro inhibitory effect of α-TEA on P. falciparum 3D7 (chloroquine-sensitive strain) and K1 (multidrug-resistant strain) was tested using a SYBR Green I-based assay. RESULTS: When 1.5% α-TEA was administered for 14 days after infection, 88% of P. berghei ANKA-infected mice survived during the experimental period. Nevertheless, all the control mice died within 12 days of infection. Furthermore, the Evans blue intensity in α-TEA-treated mice brains was less than that in untreated mice, indicating that α-TEA might inhibit the destruction of the BBB and progression of cerebral malaria. The in vitro experiment revealed that α-TEA inhibited the proliferation of both the 3D7 and K1 strains. CONCLUSION: This study showed that α-TEA is effective against murine and human malaria in vivo and in vitro, respectively. Although α-TEA alone has a sufficient antimalarial effect, future research could focus on the structure-activity relationship to achieve better pharmacokinetics and decrease the cytotoxicity and/or the combined effect of α-TEA with existing drugs. In addition, the prophylactic antimalarial activity of premedication with α-TEA may also be an interesting perspective in the future.

  • The role of autonomously secreted PGE2 and its autocrine/paracrine effect on bone matrix mineralization at the different stages of differentiating MC3T3-E1 cells.

    Hiraku Suzuki, Noriyasu Ohshima, Kazuaki Tatei, Tomoyo Taniguchi, Seiichi Sato, Takashi Izumi

    Biochemical and biophysical research communications   524 ( 4 ) 929 - 935   2020年04月 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

     概要を見る

    Bone is consisted of osteoblast-linage cells, bone-forming cells in various differentiation stages. However, it is not fully understood how communicate and interact these cells immigrated from bone marrow. In this study, we showed that prostaglandin E2 (PGE2) had a role in autonomous modification of matrix mineralization in osteoblastic cell line, MC3T3-E1, and interactions across the cells in different differentiation stages. Analysis using LC-MS/MS and inhibitors showed the autonomous secretion of PGE2 among the prostanoids in differentiation stages and that depend on COX-2, a key enzyme for production of PGE2. Treatment with inhibitors of PGE2 receptors and COX-2 indicated that secreted PGE2 regulates matrix mineralization in an autocrine/paracrine manner. In addition, we showed that the expression profile of PGE2 receptors (EP1-EP4) and PGE2 effects on matrix mineralization derived from it changed during cell differentiation. Treatment with inhibitors of PGE2 signaling in the early differentiation stage of MC3T3-E1 cells induced significant changes in matrix mineralization several days after. Stimulation with the extracts from culture medium of the matured cells including PGE2 and co-culture with the matured cells secreting PGE2 significantly promoted matrix mineralization of the early stage cells, in contrast, treatment with inhibitor of COX-2 and PGE2 receptors failed to do so. These results support that PGE2 plays important roles in the interaction system of osteoblast-linage cells in bone tissue to regulate matrix mineralization reflecting condition of bone-forming cells, that is, population and maturation.

  • Fluctuations of Spleen Cytokine and Blood Lactate, Importance of Cellular Immunity in Host Defense Against Blood Stage Malaria <i>Plasmodium yoelii</i>.

    Imai T, Suzue K, Ngo-Thanh H, Ono S, Orita W, Suzuki H, Shimokawa C, Olia A, Obi S, Taniguchi T, Ishida H, Van Kaer L, Murata S, Tanaka K, Hisaeda H

    Frontiers in immunology   10   2207 - 2207   2019年 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

     概要を見る

    Our previous studies of protective immunity and pathology against blood stage malaria parasites have shown that not only CD4+ T cells, but also CD8+ T cells and macrophages, are important for host defense against blood stage malaria infection. Furthermore, we found that Plasmodium yoelii 17XNL (PyNL) parasitizes erythroblasts, the red blood cell (RBC) precursor cells, which then express MHC class I molecules. In the present study, we analyzed spleen cytokine production. In CD8+ T cell-depleted mice, IL-10 production in early stage infection was increased over two-fold relative to infected control animals and IL-10+ CD3- cells were increased, whereas IFN-γ production in the late stage of infection was decreased. At day 16 after PyNL infection, CD8+ T cells produced more IFN-γ than CD4+ T cells. We evaluated the involvement of the immunoproteasome in induction of immune CD8+ T cells, and the role of Fas in protection against PyNL both of which are downstream of IFN-γ. In cell transfer experiments, at least the single molecules LMP7, LMP2, and PA28 are not essential for CD8+ T cell induction. The Fas mutant LPR mouse was weaker in resistance to PyNL infection than WT mice, and 20% of the animals died. LPR-derived parasitized erythroid cells exhibited less externalization of phosphatidylserine (PS), and phagocytosis by macrophages was impaired. Furthermore, we tried to identify the cause of death in malaria infection. Blood lactate concentration was increased in the CD8+ T cell-depleted PyNL-infected group at day 19 (around peak parasitemia) to similar levels as day 7 after infection with a lethal strain of Py. When we injected mice with lactate at day 4 and 6 of PyNL infection, all mice died at day 8 despite demonstrating low parasitemia, suggesting that hyperlactatemia is one of the causes of death in CD8+ T cell-depleted PyNL-infected mice. We conclude that CD8+ T cells might control cytokine production to some extent and regulate hyperparasitemia and hyperlactatemia in protection against blood stage malaria parasites.

  • A Unique Subset of γδ T Cells Expands and Produces IL-10 in Patients with Naturally Acquired Immunity against Falciparum Malaria.

    Taniguchi T, Md Mannoor K, Nonaka D, Toma H, Li C, Narita M, Vanisaveth V, Kano S, Takahashi M, Watanabe H

    Front Microbiol ( FRONTIERS MEDIA SA )  8   1288   2017年07月 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

     概要を見る

    Although expansions in gamma delta T cell populations are known to occur in the peripheral blood of patients infected with Plasmodium falciparum, the role of these cells in people with naturally acquired immunity against P. falciparum who live in malaria-endemic areas is poorly understood. We used a cross-sectional survey to investigate the role of peripheral blood gamma delta T cells in people living in Lao People's Democratic Republic, a malaria-endemic area. We found that the proportion of non-V gamma 9 gamma delta T cells was higher in non-hospitalized uncomplicated falciparum malaria patients (UMPs) from this region. Notably, we found that the non-V gamma 9 gamma delta T cells in the peripheral blood of UMPs and negative controls from this region had the potential to expand and produce IL-10 and interferon-gamma when cultured in the presence of IL-2 and/or crude P. falciparum antigens for 10 days. Furthermore, these cells were associated with plasma interleukin 10 (IL-10), which was elevated in UMPs. This is the first report demonstrating that, in UMPs living in a malaria-endemic area, a gamma delta T cell subset, the non-V gamma 9 gamma delta T cells, expands and produces IL-10. These results contribute to understanding of the mechanisms of naturally acquired immunity against P. falciparum.

  • A possible origin population of pathogenic intestinal nematodes, Strongyloides stercoralis, unveiled by molecular phylogeny

    Eiji Nagayasu, Myo Pa Pa Thet Hnin Htwe Aung, Thanaporn Hortiwakul, Akina Hino, Teruhisa Tanaka, Miwa Higashiarakawa, Alex Olia, Tomoyo Taniguchi, Soe Moe Thu Win, Isao Ohashi, Emmanuel Igwaro Odongo-Aginya, Khin Myo Aye, Mon Mon, Kyu Kyu Win, Kei Ota, Yukari Torisu, Siripen Panthuwong, Eisaku Kimura, Nirianne M. Q. Palacpac, Taisei Kikuchi, Tetsuo Hirata, Shidow Torisu, Hajime Hisaeda, Toshihiro Horii, Jiro Fujita, Wah Win Htike, Haruhiko Maruyama

    SCIENTIFIC REPORTS ( NATURE PUBLISHING GROUP )  7 ( 1 ) 4844   2017年07月 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

     概要を見る

    Humans and dogs are the two major hosts of Strongyloides stercoralis, an intestinal parasitic nematode. To better understand the phylogenetic relationships among S. stercoralis isolates infecting humans and dogs and to assess the zoonotic potential of this parasite, we analyzed mitochondrial Cox1, nuclear 18S rDNA, 28S rDNA, and a major sperm protein domain-containing protein genes. Overall, our analyses indicated the presence of two distinct lineages of S. stercoralis (referred to as type A and type B). While type A parasites were isolated both from humans and dogs in different countries, type B parasites were found exclusively in dogs, indicating that the type B has not adapted to infect humans. These epidemiological data, together with the close phylogenetic relationship of S. stercoralis with S. procyonis, a Strongyloides parasite of raccoons, possibly indicates that S. stercoralis originally evolved as a canid parasite, and later spread into humans. The inability to infect humans might be an ancestral character of this species and the type B might be surmised to be an origin population from which human-infecting strains are derived.

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著書 【 表示 / 非表示

  • 感染症と共に生きること

    谷口 委代 ( 担当: 単著 )

    月刊新医療  2018年07月

MISC(その他業績・査読無し論文等) 【 表示 / 非表示

  • マラリア感染に伴う消化器症状と腸内細菌

    谷口 委代

    臨床免疫・アレルギー科   69 ( 1 ) 107 - 113   2018年  [査読有り]

     

  • ウガンダ国の熱帯熱マラリア患者において腸内細菌叢の変化が起こる

    谷口 委代, 宮内 栄治, Olia Alex, 長安 英治, Osbert Katuro, 鈴江 一友, 今井 孝, 下川 周子, 大西 里咲, Odongo-Aginya E.I, Palacpac Nirianne, 丸山 治彦, 木村 英作, 美田 敏宏, 大野 博司, 堀井 俊宏, 久枝 一

    グローバルヘルス合同大会プログラム・抄録集 ( グローバルヘルス合同大会事務局 )  2017   192 - 192   2017年11月  [査読有り]

     

研究発表等の成果普及活動 【 表示 / 非表示

  • マラリア感染宿主における皮膚炎症状軽快化の分子メカニズム

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    第84回日本寄生虫学会大会  1900年01月  -  1900年01月   

  • 抗生剤投与により変化した腸内細菌叢はC57BL/6Nマウスにおける実験的脳マラリアを軽症化させる

    谷口 委代, 宮内 栄治, 川端 麗香, 西山 正彦, 白藤 梨可, 久枝 一, 大野 博司, 富田 治芳, 鈴木 宏志

    第91回日本寄生虫学会大会  1900年01月  -  1900年01月   

  • ネズミマラリア原虫感染による腸管病態の変化

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    第84回日本寄生虫学会大会  1900年01月  -  1900年01月   

  • A unique subset of γδ T cells expands and produces IL-10 in patients with naturally acquired immunity against Falciparum Malaria

    谷口委代

    第60回日本熱帯医学会  1900年01月  -  1900年01月   

  • Altered gut microbiota by antibiotics administration ameliorates experimental cerebral malaria in C57BL/6N mice

    Taniguchi T, Miyauchi E, Kawabata-Iwakawa R, Nishiyama M, Umemiya-Shirafuji R, Hisaeda H, Ohno H, Tomita H, Suzuki H

    15th International Congress of Parasitology  1900年01月  -  1900年01月   

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学術関係受賞 【 表示 / 非表示

  • 日本熱帯医学会研究奨励賞 受賞

    2019年03月   日本熱帯医学会  

    受賞者: 谷口委代

  • Travel Award for International Congress of Parasitology 2018

    2018年06月   日本寄生虫学会  

    受賞者: 谷口委代

  • 第21回有壬記念学術奨励賞

    2017年   新潟大学学士会  

    受賞者: 谷口委代

科研費獲得情報 【 表示 / 非表示

  • 腸内細菌を標的とした脳マラリア予防・治療法の開発に向けた研究

    基盤研究(C)

    課題番号: 23K07945

    研究期間: 2023年04月  -  2026年03月 

  • 腸内環境理解に基づく新規マラリア感染防御機構の解明

    特別研究員奨励費

    課題番号: 20J40296

    研究期間: 2020年04月  -  2023年03月 

    代表者: 谷口 委代 

    直接経費: 3,700,000(円)  間接経費: 4,810,000(円)  金額合計: 1,110,000(円)

  • マラリア感染病態への腸内細菌の作用機序の解明

    若手研究(B)

    課題番号: 00000000

    研究期間: 2016年04月  -  2020年03月 

    代表者: 谷口 委代 

    直接経費: 0(円)  間接経費: 0(円)  金額合計: 0(円)

  • マラリアにおける宿主病原体相互作用への腸内細菌の影響の解明

    若手研究(B)

    課題番号: 00000000

    研究期間: 2014年04月  -  2017年03月 

    代表者: 谷口 委代 

    直接経費: 0(円)  間接経費: 0(円)  金額合計: 0(円)

その他研究費獲得情報 【 表示 / 非表示

  • 腸内環境理解に基づく新規マラリア感染防御機構の解明

    研究費種類: 学内助成(後援会・財団含む)  参画方法: 研究代表者

    研究種別: 研究助成  事業名: 研究支援員制度

    研究期間: 2021年10月  -  2022年03月 

    代表者: 谷口委代  資金配分機関: 帯広畜産大学

  • マラリア感染の免疫記憶形成に果たす腸内細菌の役割

    研究費種類: 財団・社団法人等の民間助成金  参画方法: 研究代表者

    研究種別: 研究助成  事業名: 2019年度医学系研究助成(感染領域)

    研究期間: 2019年  -  2019年 

    代表者: 谷口委代  資金配分機関: 武田科学振興財団

  • マラリア病態形成に関わる腸内環境因子の探索

    研究費種類: 学内助成(後援会・財団含む)  参画方法: 研究代表者

    研究種別: 研究助成  事業名: 女性研究者 共同研究促進事業A型

    研究期間: 2019年  -  2019年 

    代表者: 谷口委代  資金配分機関: 群馬大学

  • 腸内環境理解に基づく新規マラリア治療・予防法の開発

    研究費種類: 財団・社団法人等の民間助成金  参画方法: 研究代表者

    研究種別: 研究助成  事業名: 内藤記念女性研究者研究助成金

    研究期間: 2018年  -  2020年 

    代表者: 谷口委代  資金配分機関: 内藤記念科学振興財団

  • マラリアにおける宿主病原体相互作用への腸内細菌の影響の解明

    研究費種類: 公的研究費(省庁・独法・大学等)  参画方法: 研究代表者

    研究種別: 研究助成  事業名: 有壬記念学術奨励賞

    研究期間: 2017年  -  2017年 

    代表者: 谷口 委代  資金配分機関: 新潟大学医学部学士会

    直接経費: 0(円)  間接経費: 0(円)  金額合計: 0(円)

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SDGs 【 表示 / 非表示

  • 2030年までに、エイズ、結核、マラリアや、これまで見放されてきた熱帯病などの伝染病をなくす目標のために、マラリアの基礎研究に取組んでいます。