Published Papers - Seo Mitsunori

Grain dormancy loss is associated with changes in ABA and GA sensitivity and hormone accumulation in bread wheat, Triticum aestivum (L.)

Keiko M. Tuttle, Shantel A. Martinez, Elizabeth C. Schramm, Yumiko Takebayashi, Mitsunori Seo, Camille M. Steber

Seed Science Research   25 ( 2 ) 179 - 193   2015.06 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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Copyright © Cambridge University Press 2015. Knowledge about the hormonal control of grain dormancy and dormancy loss is essential in wheat, because low grain dormancy at maturity is associated with the problem of pre-harvest sprouting (PHS) when cool and rainy conditions occur before harvest. Low GA (gibberellin A) hormone sensitivity and high ABA (abscisic acid) sensitivity were associated with higher wheat grain dormancy and PHS tolerance. Grains of two PHS-tolerant cultivars were very dormant at maturity, and insensitive to GA stimulation of germination. More PHS-susceptible cultivars were less sensitive to ABA inhibition of germination, and were either more GA sensitive or germinated efficiently without GA at maturity. As grain dormancy was lost through dry afterripening or cold imbibition, grains first gained GA sensitivity and then lost ABA sensitivity. These changes in GA and ABA sensitivity can serve as landmarks defining stages of dormancy loss that cannot be discerned without hormone treatment. These dormancy stages can be used to compare different cultivars, seed lots and studies. Previous work showed that wheat afterripening is associated with decreasing ABA levels in imbibing seeds. Wheat grain dormancy loss through cold imbibition also led to decreased endogenous ABA levels, suggesting that reduced ABA signalling is a general mechanism triggering dormancy loss.

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The jasmonate-responsive GTR1 transporter is required for gibberellin-mediated stamen development in Arabidopsis

Hikaru Saito, Takaya Oikawa, Shin Hamamoto, Yasuhiro Ishimaru, Miyu Kanamori-Sato, Yuko Sasaki-Sekimoto, Tomoya Utsumi, Jing Chen, Yuri Kanno, Shinji Masuda, Yuji Kamiya, Mitsunori Seo, Nobuyuki Uozumi, Minoru Ueda, Hiroyuki Ohta

Nature Communications   6   2015.02 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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© 2015 Macmillan Publishers Limited. All rights reserved. Plant hormones are transported across cell membranes during various physiological events. Recent identification of abscisic acid and strigolactone transporters suggests that transport of various plant hormones across membranes does not occur by simple diffusion but requires transporter proteins that are strictly regulated during development. Here, we report that a major glucosinolate transporter, GTR1/NPF2.10, is multifunctional and may be involved in hormone transport in Arabidopsis thaliana. When heterologously expressed in oocytes, GTR1 transports jasmonoyl-isoleucine and gibberellin in addition to glucosinolates. gtr1 mutants are severely impaired in filament elongation and anther dehiscence resulting in reduced fertility, but these phenotypes can be rescued by gibberellin treatment. These results suggest that GTR1 may be a multifunctional transporter for the structurally distinct compounds glucosinolates, jasmonoyl-isoleucine and gibberellin, and may positively regulate stamen development by mediating gibberellin supply.

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A plant u-box protein, pub4, regulates asymmetric cell division and cell proliferation in the root meristem

Atsuko Kinoshita, Colette A. Ten Hove, Ryo Tabata, Masashi Yamada, Noriko Shimizu, Takashi Ishida, Katsushi Yamaguchi, Shuji Shigenobu, Yumiko Takebayashi, Satoshi Iuchi, Masatomo Kobayashi, Tetsuya Kurata, Takuji Wada, Mitsunori Seo, Mitsuyasu Hasebe, Ikram Blilou, Hiroo Fukuda, Ben Scheres, Renze Heidstra, Yuji Kamiya, Shinichiro Sawa

Development (Cambridge)   142 ( 3 ) 444 - 453   2015.02 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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© 2015. Published by The Company of Biologists Ltd. The root meristem (RM) is a fundamental structure that is responsible for postembryonic root growth. The RMc ontains the quiescent center (QC), stem cells and frequently dividing meristematic cells, in which the timing and the frequency of cell division are tightly regulated. In Arabidopsis thaliana, several gain-of-function analyses have demonstrated that peptide ligands of the CLAVATA3 (CLV3)/EMBRYO SURROUNDING REGION-RELATED (CLE) family are important for maintaining RM size. Here, we demonstrate that a plant U-box E3 ubiquitin ligase, PUB4, isanovel downstream component of CLV3/CLE signaling in the RM. Mutations in PUB4 reduced the inhibitory effect of exogenous CLV3/CLE peptide on root cell proliferation and columella stem cell maintenance. Moreover, pub4 mutants grown without exogenous CLV3/CLE peptide exhibited characteristic phenotypes in the RM, such as enhanced root growth, increased number of cortex/endodermis stem cells and decreased number of columella layers. Our phenotypic and gene expression analyses indicated that PUB4 promotes expression of a cell cycle regulatory gene, CYCD6;1, and regulates formative periclinal asymmetric cell divisions in endodermis and cortex/ endodermis initial daughters. These data suggest that PUB4 functions as a global regulator of cell proliferation and the timing of asymmetric cell division that are important for final root architecture.

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Arabidopsis cold shock domain protein 2 influences ABA accumulation in seed and negatively regulates germination

Kentaro Sasaki, Myung Hee Kim, Yuri Kanno, Mitsunori Seo, Yuji Kamiya, Ryozo Imai

Biochemical and Biophysical Research Communications   456 ( 1 ) 380 - 384   2015.01 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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© 2014 Elsevier Inc. All rights reserved. The cold shock domain (CSD) is the most conserved nucleic acid binding domain and is distributed from bacteria to animals and plants. CSD proteins are RNA chaperones that destabilize RNA secondary structures to regulate stress tolerance and development. AtCSP2 is one of the four CSD proteins in Arabidopsis and is up-regulated in response to cold. Since AtCSP2 negatively regulates freezing tolerance, it was proposed to be a modulator of freezing tolerance during cold acclimation. Here, we examined the function of AtCSP2 in seed germination. We found that AtCSP2-overexpressing lines demonstrated retarded germination as compared with the wild type, with or without stress treatments. The ABA levels in AtCSP2-overexpressing seeds were higher than those in the wild type. In addition, overexpression of AtCSP2 reduced the expression of an ABA catabolic gene (CYP707A2) and gibberellin biosynthesis genes (GA20ox and GA3ox). These results suggest that AtCSP2 negatively regulates seed germination by controlling ABA and GA levels.

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Mystery in genetics: PUB4 gives a clue to the complex mechanism of CLV signaling pathway in the shoot apical meristem

Atsuko Kinoshita, Mitsunori Seo, Yuji Kamiya, Shinichiro Sawa

Plant Signaling and Behavior   10 ( 6 )   2015.01 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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© 2015, Taylor & Francis Group, LLC. Postembryonic growth and development in higher plants are ultimately reliant on the activity of meristems, where the cells divide frequently to provide source cells for new organs and tissues while in part maintain their pluripotent nature as stem cells. The shoot apical meristem (SAM) is maintained throughout the life of plants and responsible for the development of all areal tissues. In Arabidopsis thaliana, the size of SAM is controlled by a peptide ligand, CLAVATA3 (CLV3). Previously, genetic studies have identified several genes that function downstream of CLV3, many of which, intriguingly, encode receptors. Recently we identified an E3 ubiquitin ligase, PLANT U-BOX 4 (PUB4), as a key regulatory component of root meristem maintenance that functions downstream of an exogenous synthetic CLV3 peptide. Here, we report an additional function of PUB4 in the SAM.

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Live single-cell plant hormone analysis by video-mass spectrometry

Takafumi Shimizu, Shinya Miyakawa, Tsuyoshi Esaki, Hajime Mizuno, Tsutomu Masujima, Tomokazu Koshiba, Mitsunori Seo

Plant and Cell Physiology   56 ( 7 ) 1287 - 1296   2015 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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© The Author 2015. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. Studies have indicated that endogenous concentrations of plant hormones are regulated very locally within plants. To understand the mechanisms underlying hormone-mediated physiological processes, it is indispensable to know the exact hormone concentrations at cellular levels. In the present study, we established a system to determine levels of ABA and jasmo-noyl-isoleucine (JA-Ile) from single cells. Samples taken from a cell of Vicia faba leaves using nano-electrospray ionization (ESI) tips under a microscope were directly introduced into mass spectrometers by infusion and subjected to tandem mass spec-trometry (MS/MS) analysis. Stable isotope-labeled [D<inf>6</inf>]ABA or [¹³C<inf>6</inf>]JA-Ile was used as an internal standard to compensate ionization efficiencies, which determine the amount of ions introduced into mass spectrometers. We detected ABA and JA-Ile from single cells of water- and wound-stressed leaves, whereas they were almost undetectable in non-stressed single cells. The levels of ABA and JA-Ile found in the single-cell analysis were compared with levels found by analysis of purified extracts with liquid chromatography-tandem mass spectrometry (LC-MS/MS). These results demonstrated that stress-induced accumulation of ABA and JA-Ile could be monitored from living single cells.

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Loss of Arabidopsis thaliana Seed Dormancy is Associated with Increased Accumulation of the GID1 GA Hormone Receptors

Amber L. Hauvermale, Keiko M. Tuttle, Yumiko Takebayashi, Mitsunori Seo, Camille M. Steber

Plant and Cell Physiology   56 ( 9 ) 1773 - 1785   2014.10 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

Salt adaptation requires efficient fine-tuning of jasmonate signalling

Ahmed Ismail, Mitsunori Seo, Yumiko Takebayashi, Yuji Kamiya, Elisabeth Eiche, Peter Nick

Protoplasma   251 ( 4 ) 881 - 898   2014.07 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

Designed abscisic acid analogs as antagonists of PYL-PP2C receptor interactions

Jun Takeuchi, Masanori Okamoto, Tomonori Akiyama, Takuya Muto, Shunsuke Yajima, Masayuki Sue, Mitsunori Seo, Yuri Kanno, Tsunashi Kamo, Akira Endo, Eiji Nambara, Nobuhiro Hirai, Toshiyuki Ohnishi, Sean R. Cutler, Yasushi Todoroki

Nature Chemical Biology   10 ( 6 ) 477 - 482   2014.06 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

Wound-induced expression of DEFECTIVE IN ANTHER DEHISCENCE1 and DAD1-like lipase genes is mediated by both CORONATINE INSENSITIVE1-dependent and independent pathways in Arabidopsis thaliana

Izabela Ruduś, Haruka Terai, Takafumi Shimizu, Hisae Kojima, Kazuki Hattori, Yuka Nishimori, Hironaka Tsukagoshi, Yuji Kamiya, Mitsunori Seo, Kenzo Nakamura, Jan Kępczyński, Sumie Ishiguro

Plant Cell Reports   33 ( 6 ) 849 - 860   2014.06 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

Klebsormidium flaccidum genome reveals primary factors for plant terrestrial adaptation

Koichi Hori, Fumito Maruyama, Takatomo Fujisawa, Tomoaki Togashi, Nozomi Yamamoto, Mitsunori Seo, Syusei Sato, Takuji Yamada, Hiroshi Mori, Naoyuki Tajima, Takashi Moriyama, Masahiko Ikeuchi, Mai Watanabe, Hajime Wada, Koichi Kobayashi, Masakazu Saito, Tatsuru Masuda, Yuko Sasaki-Sekimoto, Kiyoshi Mashiguchi, Koichiro Awai, Mie Shimojima, Shinji Masuda, Masako Iwai, Takashi Nobusawa, Takafumi Narise, Satoshi Kondo, Hikaru Saito, Ryoichi Sato, Masato Murakawa, Yuta Ihara, Yui Oshima-Yamada, Kinuka Ohtaka, Masanori Satoh, Kohei Sonobe, Midori Ishii, Ryosuke Ohtani, Miyu Kanamori-Sato, Rina Honoki, Daichi Miyazaki, Hitoshi Mochizuki, Jumpei Umetsu, Kouichi Higashi, Daisuke Shibata, Yuji Kamiya, Naoki Sato, Yasukazu Nakamura, Satoshi Tabata, Shigeru Ida, Ken Kurokawa, Hiroyuki Ohta

Nature Communications   5   3987   2014.05 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

Gibberellin acts positively then negatively to control onset of flower formation in Arabidopsis

Nobutoshi Yamaguchi, Cara M. Winter, Miin Feng Wu, Yuri Kanno, Ayako Yamaguchi, Mitsunori Seo, Doris Wagner

Science   344 ( 6184 ) 638 - 641   2014 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family members in plants

Léran S, Varala K, Boyer JC, Chiurazzi M, Crawford N, Daniel-Vedèle F, David L, Dickstein R, Fernandez E, Forde B, Gassmann W, Geiger D, Gojon A, Gong JM, Halkier BA, Harris JM, Hedrich R, Limami AM, Rentsch D, Seo M, Tsay YF, Zhang M, Coruzzi G, Lacombe B

Trends in Plant Science   19   5 - 9   2014 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

Combining association mapping and transcriptomics identify HD2B histone deacetylase as a genetic factor associated with seed dormancy in Arabidopsis thaliana

Ryoichi Yano, Yumiko Takebayashi, Eiji Nambara, Yuji Kamiya, Mitsunori Seo

Plant Journal   74 ( 5 ) 815 - 828   2013.06 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

Regulation of Wheat Seed Dormancy by After-Ripening Is Mediated by Specific Transcriptional Switches That Induce Changes in Seed Hormone Metabolism and Signaling

Aihua Liu, Feng Gao, Yuri Kanno, Mark C. Jordan, Yuji Kamiya, Mitsunori Seo, Belay T. Ayele

PLoS ONE   8 ( 2 )   2013.02 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

Nitrate does not compete with abscisic acid as a substrate of AtNPF4.6/NRT1.2/AIT1 in Arabidopsis

Yuri Kanno, Yuji Kamiya, Mitsunori Seo

Plant Signaling and Behavior   8 ( 12 )   2013 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

The time required for dormancy release in arabidopsis is determined by DELAY OF GERMINATION1 protein levels in freshly harvested seeds

Kazumi Nakabayashi, Melanie Bartsch, Yong Xiang, Emma Miatton, Silke Pellengahr, Ryoichi Yano, Mitsunori Seo, Wim J.J. Soppe

Plant Cell   24 ( 7 ) 2826 - 2838   2012.07 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

Identification of an abscisic acid transporter by functional screening using the receptor complex as a sensor

Yuri Kanno, Atsushi Hanada, Yasutaka Chiba, Takanari Ichikawa, Miki Nakazawa, Minami Matsui, Tomokazu Koshiba, Yuji Kamiya, Mitsunori Seo

Proceedings of the National Academy of Sciences of the United States of America   109 ( 24 ) 9653 - 9658   2012.06 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

A vacuolar β-Glucosidase homolog that possesses glucose-conjugated abscisic acid hydrolyzing activity plays an important role in osmotic stress responses in Arabidopsis

Zheng Yi Xu, Kwang Hee Lee, Ting Dong, Jae Cheol Jeong, Jing Bo Jin, Yuri Kanno, Dae Heon Kim, Soo Youn Kim, Mitsunori Seo, Ray A. Bressan, Dae Jin Yun, Inhwan Hwang

Plant Cell   24 ( 5 ) 2184 - 2199   2012.05 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

Epoxycarotenoid cleavage by NCED5 fine-tunes ABA accumulation and affects seed dormancy and drought tolerance with other NCED family members

Anne Frey, Delphine Effroy, Valérie Lefebvre, Mitsunori Seo, François Perreau, Adeline Berger, Julien Sechet, Alexandra To, Helen M. North, Annie Marion-Poll

Plant Journal   70 ( 3 ) 501 - 512   2012.05 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)