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• Current regenerative medicine-based approaches for skin regeneration: A review of literature and a report on clinical applications in Japan.

  Shimizu Y, Ntege EH, Sunami H

  Regenerative therapy ( Regenerative Therapy )  21   73 - 80   2022.12 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

  　View Summary

  Current trends indicate a growing interest among healthcare specialists and the public in the use of regenerative medicine-based approaches for skin regeneration. The approaches are categorised in either cell-based or cell-free therapies and are reportedly safe and effective. Cell-based therapies include mesenchymal stem cells (MSCs), tissue induced pluripotent stem cells (iPSCs), fibroblast-based products, and blood-derived therapies, such as those employing platelet-rich plasma (PRP) products. Cell-free therapies primarily involve the use of MSC-derived extracellular vesicles/exosomes. MSCs are isolated from various tissues, such as fat, bone marrow, umbilical cord, menstrual blood, and foetal skin, and expanded ex vivo before transplantation. In cell-free therapies, MSC exosomes, MSC-derived cultured media, and MSC-derived extracellular vesicles are collected from MSC-conditioned media or supernatant. In this review, a literature search of the Cochrane Library, MEDLINE (PubMed), EMBASE, and Scopus was conducted using several combinations of terms, such as 'stem', 'cell', 'aging', 'wrinkles', 'nasolabial folds', 'therapy', 'mesenchymal stem cells', and 'skin', to identify relevant articles providing a comprehensive update on the different regenerative medicine-based therapies and their application to skin regeneration. In addition, the regulatory perspectives on the clinical application of some of these therapies in Japan are highlighted.

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• A morphological study of adipose-derived stem cell sheets created with temperature-responsive culture dishes using scanning electron microscopy.

  Taki Y, Fuku A, Nakamura Y, Koya T, Kitajima H, Tanida I, Takaki T, Nozaki K, Sunami H, Hirata H, Tachi Y, Shimasaki T, Masauji T, Yamamoto N, Ishigaki Y, Shimodaira S, Shimizu Y, Ichiseki T, Kaneuji A, Osawa S, Kawahara N

  Medical molecular morphology ( Medical Molecular Morphology )  55 ( 3 ) 187 - 198   2022.09 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  Adipose-derived stem cell (ADSC) sheets have potential to be effective in various therapies. In this study, we first demonstrated that a cell sheet composed of human ADSCs could be created using a new temperature-responsive culture dish from the DIC Corporation. The dish can cause detachment of adherent cells due to temperature changes, but a few morphological analyses have evaluated the presence or absence of damage on the detached surface of cell sheet. To characterize our ADSC sheet, we tried to observe the surface of ADSC sheets with scanning electron microscope (SEM) using the ionic liquid, which enables the rapid preparation of samples. No damage was found on the surface of the ADSC sheets on the side that had been in contact with the surface of the culture dishes. In addition, when the transcriptomes of the harvested cell sheets were compared with those of monolayer cultures, no up-regulation of cell death related genes were detected. These results propose that the detachment from temperature-responsive culture dish causes no serious damage on the prepared ADSC sheet. It is also suggested that the SEM with ionic liquids is a useful and rapid method for the analysis of ADSC sheets for therapy.

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• An unusual case of oral surgical management in a patient with isovaleric acidemia and schizophrenia: A case report.

  Miyamoto S, Ntege EH, Chinen Y, Goto T, Shirakawa J, Goto S, Kawano T, Shimizu Y, Nakanishi K, Nakamura H

  Biomedical reports ( Biomedical Reports )  17 ( 2 ) 64 - 64   2022.08 [ Peer Review Accepted ]

  Type of publication: Research paper (other science council materials etc.)

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  Oral/dental surgical care in patients with chronic medical comorbidities, such as isovaleric acidemia (IVA), can be challenging. In addition to technical complications, different comorbidities also present a complex range of concerning factors/challenges, which can increase the incidence of morbidity and mortality associated with surgery. IVA, a congenital error of metabolism, is a rare organic acidemia with a predisposition towards acute acidosis and life-threatening metabolic decompensation during stressful conditions, such as prolonged fasting and surgery. In addition, schizophrenia, a major neurological disorder, can result in manifestation of severe dental or periodontal conditions, including pericoronitis. The condition is associated with significant risk factors of postoperative complications, such as dangerous behaviors and adverse interactions between antipsychotic drugs and anesthetic agents. A case of comorbid dental disease with two coexisting chronic and life-threatening medical conditions, one of which is rare, is an unusual encounter in oral/dental surgery that is seldomly published. Moreover, implementing a safe and effective surgical intervention in such patients requires several informed considerations. However, only a few reported experiences or guidelines exist, reporting appropriate perioperative management strategies to minimize risks. Hence, in this case report, our experience of managing one of these rare encounters of a 20-year-old man who suffered from bilaterally partially erupted third molars, associated with chronic pericoronitis and dental caries of both the maxilla wisdom teeth with coexisting IVA and schizophrenia comorbidities is described. Additionally, the presentation and anticipated complications of the comorbid disorders of the patient are briefly reviewed. In this case, the pericoronitis and dental caries were treated by surgically removing the impacted third molars and the antagonist maxilla wisdom teeth under regional anesthesia and application of antibiotics for 3 days. The patient recovered without any postoperative complications after 1 year of follow-up.

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• Acquired Cutis Laxa on the Upper Eyelids and Earlobes: A Case Report and Literature Review.

  Katsuren K, Kuba R, Kasai S, Shimizu Y

  Archives of plastic surgery   49 ( 3 ) 418 - 422   2022.05 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  Acquired cutis laxa is a rare disease. Owing to few reports on the condition, no statistical data have been produced. Cutis laxa is characterized by drooping skin, caused by decreased levels of dermal elastin, leading to reduced skin elasticity. The disease usually emerges on the neck or trunk and spreads throughout the body; however, it rarely involves the extremities. 2 Moreover, cases localized to the face are rare. The objective of this clinical case report was to highlight this unusual disease in a 24-year-old female, with localization on the face and neck. The patient underwent surgery for treatment of bilateral ear lobe and eyelid skin laxity.

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• Revised donor site skin incision technique for a multivector functioning muscle transfer using the serratus anterior muscle for smile reanimation.

  Shimizu Y, Kasai S, Asato R, Matsuura N, Katsuren S, Fukuda R

  Journal of plastic, reconstructive & aesthetic surgery : JPRAS ( Journal of Plastic, Reconstructive and Aesthetic Surgery )  75 ( 4 ) 1497 - 1520   2022.04 [ Peer Review Accepted ]

  Type of publication: Research paper (other science council materials etc.)

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Grant-in-Aid for Scientific Research 【 display / non-display 】

Grant-in-Aid for Scientific Research 【 display / non-display 】

• Development of a new microscopic observation method for cross-sectional observation of living cell membrane structures

  Challenging research (sprout)

  Project Year: 2022.06  -  2025.03 

  Direct: 4,900,000 (YEN)  Overheads: 6,370,000 (YEN)  Total: 1,470,000 (YEN)

• Development of new preventive method for postoperative paraplegia of thoracoabdominal aorta using exosomes

  Grant-in-Aid for Scientific Research(C)

  Project Year: 2022.04  -  2025.03 

  Direct: 3,200,000 (YEN)  Overheads: 4,160,000 (YEN)  Total: 960,000 (YEN)

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2022.04  -  2025.03 

  Direct: 3,200,000 (YEN)  Overheads: 4,160,000 (YEN)  Total: 960,000 (YEN)

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2019.04  -  2022.03 

• Grant-in-Aid for Scientific Research(B)

  Project Year: 2019.04  -  2022.03 

  Direct: 13,400,000 (YEN)  Overheads: 17,420,000 (YEN)  Total: 4,020,000 (YEN)

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