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• Current regenerative medicine-based approaches for skin regeneration: A review of literature and a report on clinical applications in Japan.

  Shimizu Y, Ntege EH, Sunami H

  Regenerative therapy ( Regenerative Therapy )  21   73 - 80   2022年12月 [ 査読有り ]

  掲載種別: 研究論文（学術雑誌）

  　概要を見る

  Current trends indicate a growing interest among healthcare specialists and the public in the use of regenerative medicine-based approaches for skin regeneration. The approaches are categorised in either cell-based or cell-free therapies and are reportedly safe and effective. Cell-based therapies include mesenchymal stem cells (MSCs), tissue induced pluripotent stem cells (iPSCs), fibroblast-based products, and blood-derived therapies, such as those employing platelet-rich plasma (PRP) products. Cell-free therapies primarily involve the use of MSC-derived extracellular vesicles/exosomes. MSCs are isolated from various tissues, such as fat, bone marrow, umbilical cord, menstrual blood, and foetal skin, and expanded ex vivo before transplantation. In cell-free therapies, MSC exosomes, MSC-derived cultured media, and MSC-derived extracellular vesicles are collected from MSC-conditioned media or supernatant. In this review, a literature search of the Cochrane Library, MEDLINE (PubMed), EMBASE, and Scopus was conducted using several combinations of terms, such as 'stem', 'cell', 'aging', 'wrinkles', 'nasolabial folds', 'therapy', 'mesenchymal stem cells', and 'skin', to identify relevant articles providing a comprehensive update on the different regenerative medicine-based therapies and their application to skin regeneration. In addition, the regulatory perspectives on the clinical application of some of these therapies in Japan are highlighted.

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• A morphological study of adipose-derived stem cell sheets created with temperature-responsive culture dishes using scanning electron microscopy.

  Taki Y, Fuku A, Nakamura Y, Koya T, Kitajima H, Tanida I, Takaki T, Nozaki K, Sunami H, Hirata H, Tachi Y, Shimasaki T, Masauji T, Yamamoto N, Ishigaki Y, Shimodaira S, Shimizu Y, Ichiseki T, Kaneuji A, Osawa S, Kawahara N

  Medical molecular morphology ( Medical Molecular Morphology )  55 ( 3 ) 187 - 198   2022年09月 [ 査読有り ]

  掲載種別: 研究論文（学術雑誌）

  　概要を見る

  Adipose-derived stem cell (ADSC) sheets have potential to be effective in various therapies. In this study, we first demonstrated that a cell sheet composed of human ADSCs could be created using a new temperature-responsive culture dish from the DIC Corporation. The dish can cause detachment of adherent cells due to temperature changes, but a few morphological analyses have evaluated the presence or absence of damage on the detached surface of cell sheet. To characterize our ADSC sheet, we tried to observe the surface of ADSC sheets with scanning electron microscope (SEM) using the ionic liquid, which enables the rapid preparation of samples. No damage was found on the surface of the ADSC sheets on the side that had been in contact with the surface of the culture dishes. In addition, when the transcriptomes of the harvested cell sheets were compared with those of monolayer cultures, no up-regulation of cell death related genes were detected. These results propose that the detachment from temperature-responsive culture dish causes no serious damage on the prepared ADSC sheet. It is also suggested that the SEM with ionic liquids is a useful and rapid method for the analysis of ADSC sheets for therapy.

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• An unusual case of oral surgical management in a patient with isovaleric acidemia and schizophrenia: A case report.

  Miyamoto S, Ntege EH, Chinen Y, Goto T, Shirakawa J, Goto S, Kawano T, Shimizu Y, Nakanishi K, Nakamura H

  Biomedical reports ( Biomedical Reports )  17 ( 2 ) 64 - 64   2022年08月 [ 査読有り ]

  掲載種別: 研究論文（その他学術会議資料等）

  　概要を見る

  Oral/dental surgical care in patients with chronic medical comorbidities, such as isovaleric acidemia (IVA), can be challenging. In addition to technical complications, different comorbidities also present a complex range of concerning factors/challenges, which can increase the incidence of morbidity and mortality associated with surgery. IVA, a congenital error of metabolism, is a rare organic acidemia with a predisposition towards acute acidosis and life-threatening metabolic decompensation during stressful conditions, such as prolonged fasting and surgery. In addition, schizophrenia, a major neurological disorder, can result in manifestation of severe dental or periodontal conditions, including pericoronitis. The condition is associated with significant risk factors of postoperative complications, such as dangerous behaviors and adverse interactions between antipsychotic drugs and anesthetic agents. A case of comorbid dental disease with two coexisting chronic and life-threatening medical conditions, one of which is rare, is an unusual encounter in oral/dental surgery that is seldomly published. Moreover, implementing a safe and effective surgical intervention in such patients requires several informed considerations. However, only a few reported experiences or guidelines exist, reporting appropriate perioperative management strategies to minimize risks. Hence, in this case report, our experience of managing one of these rare encounters of a 20-year-old man who suffered from bilaterally partially erupted third molars, associated with chronic pericoronitis and dental caries of both the maxilla wisdom teeth with coexisting IVA and schizophrenia comorbidities is described. Additionally, the presentation and anticipated complications of the comorbid disorders of the patient are briefly reviewed. In this case, the pericoronitis and dental caries were treated by surgically removing the impacted third molars and the antagonist maxilla wisdom teeth under regional anesthesia and application of antibiotics for 3 days. The patient recovered without any postoperative complications after 1 year of follow-up.

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• Acquired Cutis Laxa on the Upper Eyelids and Earlobes: A Case Report and Literature Review.

  Katsuren K, Kuba R, Kasai S, Shimizu Y

  Archives of plastic surgery   49 ( 3 ) 418 - 422   2022年05月 [ 査読有り ]

  掲載種別: 研究論文（学術雑誌）

  　概要を見る

  Acquired cutis laxa is a rare disease. Owing to few reports on the condition, no statistical data have been produced. Cutis laxa is characterized by drooping skin, caused by decreased levels of dermal elastin, leading to reduced skin elasticity. The disease usually emerges on the neck or trunk and spreads throughout the body; however, it rarely involves the extremities. 2 Moreover, cases localized to the face are rare. The objective of this clinical case report was to highlight this unusual disease in a 24-year-old female, with localization on the face and neck. The patient underwent surgery for treatment of bilateral ear lobe and eyelid skin laxity.

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• Revised donor site skin incision technique for a multivector functioning muscle transfer using the serratus anterior muscle for smile reanimation.

  Shimizu Y, Kasai S, Asato R, Matsuura N, Katsuren S, Fukuda R

  Journal of plastic, reconstructive & aesthetic surgery : JPRAS ( Journal of Plastic, Reconstructive and Aesthetic Surgery )  75 ( 4 ) 1497 - 1520   2022年04月 [ 査読有り ]

  掲載種別: 研究論文（その他学術会議資料等）

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• コラゲナーゼを用いないで脂肪組織由来幹細胞を分離抽出するための方法、及び脂肪由来幹細胞分離抽出用キット

  特願 特願PCT/JP2019/007472  (2019年02月27日)

  角南寛, 清水雄介, 普天間直子, 牧田昌士, 大坂直也

• 脂質二重膜に覆われた微粒子又は細胞外小胞の回収方法および脂質二重膜に覆われた微粒子または細胞外小胞の回収キット

  特開 2022-057872  (2022年03月31日)

  清水雄介、角南寛、外部機関

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• 生きた細胞膜構造を断面観察する新しい顕微観察法の開発

  挑戦的研究(萌芽)

  課題番号: 22K19298

  研究期間: 2022年06月  -  2025年03月 

  代表者: 角南 寛, 清水 雄介 

  直接経費: 4,900,000（円）  間接経費: 6,370,000（円）  金額合計: 1,470,000（円）

• エクソソームを用いた胸腹部大動脈術後対麻痺に対する新たな予防法の開発

  基盤研究(C)

  課題番号: 22K08940

  研究期間: 2022年04月  -  2025年03月 

  代表者: 清水 雄介, 神里 興太, 角南 寛 

  直接経費: 3,200,000（円）  間接経費: 4,160,000（円）  金額合計: 960,000（円）

• リンパ浮腫治療のための脂肪間質細胞改変型”Designer Cells”の開発

  基盤研究(C)

  課題番号: 22K09885

  研究期間: 2022年04月  -  2025年03月 

  代表者: 市瀬 多恵子, 市瀬 広武, 清水 雄介 

  直接経費: 3,200,000（円）  間接経費: 4,160,000（円）  金額合計: 960,000（円）

• 琉球大学を起点としたヒト細胞原料供給体制の実装

  ■■■

  課題番号: 00000000

  研究期間: 2021年07月  -  2024年03月 

  代表者: 清水雄介, 中村博幸, 植田真一郎, 銘苅桂子, 平田哲生, 東千夏, 池原由美, 角南寛 

• 脂肪幹細胞由来神経細胞による胸腹部大動脈術後虚血性対麻痺に対する新規治療法の開発

  基盤研究(C)

  課題番号: 19K09248

  研究期間: 2019年04月  -  2022年03月 

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• 琉球大学を起点としたヒト（同種）体性幹細胞原料の安定供給システムの構築

  研究費種類: 公的研究費（省庁・独法・大学等）  参画方法: 研究代表者

  研究種別: 受託研究  事業名: 再生医療・遺伝子治療の産業化に向けた基盤技術開発事業

  研究期間: 2018年10月  -  2021年03月 

  資金配分機関: 国立研究開発法人日本医療研究開発機構

  直接経費: 90,000,000（円）  間接経費: 27,000,000（円）  金額合計: 117,000,000（円）