Current Affiliation Organization 【 display / non-display 】

Current Affiliation Organization 【 display / non-display 】

University 【 display / non-display 】

University 【 display / non-display 】

Graduate School 【 display / non-display 】

Graduate School 【 display / non-display 】

Academic degree 【 display / non-display 】

Academic degree 【 display / non-display 】

External Career 【 display / non-display 】

External Career 【 display / non-display 】

Affiliated academic organizations 【 display / non-display 】

Affiliated academic organizations 【 display / non-display 】

Research Interests 【 display / non-display 】

Research Interests 【 display / non-display 】

display all >>

Research Areas 【 display / non-display 】

Research Areas 【 display / non-display 】

Research Theme 【 display / non-display 】

Research Theme 【 display / non-display 】

• Latitudinal cline in species diveristy of Oryzias

• Latitudinal cline in sexual selection in Oryzias spp.

• Latitudinal variation in life histories of Oryzias spp.

Published Papers 【 display / non-display 】

Published Papers 【 display / non-display 】

• A Simple Method for RNA-Seq of Manually Isolated Chromatophores in Oryzias Fishes.

  Makoto Goda, Asuka Miyagi, Keisuke Sugiwaka, Masakatsu Watanabe, Manabu Bessho-Uehara, Masahiko Hibi, Atsushi Toyoda, Rieko Tanaka, Kawilarang W A Masengi, Kazunori Yamahira, Satoshi Ansai, Hisashi Hashimoto

  Development, growth & differentiation   68 ( 3 ) e70044   2026.04 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

  　View Summary

  RNA sequencing (RNA-seq) has become an essential tool for analyzing gene expression and exploring cell type-specific transcriptomes. However, sample preparation and quality control remain challenging, as current approaches typically rely on dissecting tissues containing mixed cell populations or using flow cytometry to isolate fluorescently labeled cells. Here we present a simple and reliable method for RNA-seq of chromatophores (pigment cells) by manually isolating cells based on their natural pigmentation. We analyzed four chromatophore types-melanophores, xanthophores, iridophores, and leucophores-in medaka (Oryzias latipes). Remarkably, as few as 100 cells per type yielded reasonably high-quality transcriptomes sufficient to identify differentially expressed genes (DEGs). Furthermore, this method was successfully applied to a non-model medaka species, O. woworae, which shares the same four chromatophore types. Our approach enables efficient, low-cost, and cross-species transcriptome analysis of chromatophores without requiring transgenic markers or flow cytometry.

  • Access this article

    DOI
  • Search related information
    PubMed

• Genetic and body-shape divergence of the ricefish <i>Oryzias nigrimas</i> between populations in Lake Poso and the outflowing Poso River, Sulawesi, Indonesia

  Ixchel F. Mandagi, Akira W. R. Masengi, Hirozumi Kobayashi, Handung Nuryadi, Kawilarang W. A. Masengi, Kazunori Yamahira, Ryo Kakioka

  ICHTHYOLOGICAL RESEARCH     2026.01 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

  • Access this article

    DOI

• Routine mitochondrial recombination drives rapid concerted evolution of duplicated control regions in a wild fish

  Handung Nuryadi, V. K. Anoop, Ryo Kakioka, Jun Gojobori, Rajeev Raghavan, Kazunori Yamahira

  HEREDITY     2025.12 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

  • Access this article

    DOI

• Shorter Recruitment Periods and Higher Fecundity in High-Latitude Populations of the Oryzias latipes Species Complex

  Shingo Fujimoto, Iki Murase, Hirozumi Kobayashi, Bayu K. A. Sumarto, Mitsuharu Yagi, Kazunori Yamahira

  POPULATION ECOLOGY   68 ( 1 )   2025.12 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

  • Access this article

    DOI

• Population structure of the western Indian endemic medaka, <i>Oryzias setnai</i>, inferred from mitochondrial haplotypes

  V. K. Anoop, Handung Nuryadi, Rajeev Raghavan, Kazunori Yamahira

  ICHTHYOLOGICAL RESEARCH   72 ( 4 ) 466 - 470   2025.11 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

  • Access this article

    DOI

display all >>

Books 【 display / non-display 】

Books 【 display / non-display 】

• Medaka: Biology, Management, and Experimental Protocols (Chapter 2: Medaka and Oryzias species as model organisms and the current status of medaka biological resources)

  山平 寿智 ( Part: Allotment Writing )

  ■■■  2019.11

Other Papers 【 display / non-display 】

Other Papers 【 display / non-display 】

• Different evolutionary histories of cave fishes on the same island

  小林大純, MASENGI Kawilarang W. A., 山平寿智

  日本生態学会大会講演要旨(Web)   68th   2021

   

  J-GLOBAL

• Fitness effects of a gene for sexual dimorphism revealed by genome editing in a Sulawesian fish

  安齋賢, 安齋賢, 安齋賢, 持田浩治, 持田浩治, 藤本真悟, MOKODONGAN Daniel F., MOKODONGAN Daniel F., SUMARTO Bayu Kreshna Adhitya, MASENGI Kawilarang W. A., HADIATY Renny K., 永野惇, 豊田敦, 成瀬清, 山平寿智, 北野潤

  日本生態学会大会講演要旨(Web)   68th   2021

   

  J-GLOBAL

• Non-migratory ecology and systematics of ′′Redigobius penango′′ in Sulawesi

  小林大純, 袰岩美月, MOKODONGAN Daniel F., 安田仁奈, 白井厚太朗, 飯田碧, 山平寿智

  日本魚類学会年会講演要旨   54th (CD-ROM)   2020

   

  J-GLOBAL

• Speciation of medaka fishes in ancient lakes of Sulawesi

  山平寿智

  日本生態学会大会講演要旨(Web)   67th   2020

   

  J-GLOBAL

• Study on existence of the fisheries resources abundance by using environmental deoxyribonucleic acid (e-DNA) approach at fishing grounds in the Sulawesi Sea, Indonesia

  Masengi K.

  IOP Conference Series: Materials Science and Engineering ( IOP Conference Series: Materials Science and Engineering )  567 ( 1 )   2019.08  [Refereed]

   

  DOI

display all >>

Grant-in-Aid for Scientific Research 【 display / non-display 】

Grant-in-Aid for Scientific Research 【 display / non-display 】

• Challenging research (development)

  Project Year: 2025.06  -  2028.03 

  Direct: 19,900,000 (YEN)  Overheads: 25,870,000 (YEN)  Total: 5,970,000 (YEN)

• Verification of a novel mode of speciation by mitochondria originated from a different species

  Grant-in-Aid for Scientific Research(A)

  Project Year: 2025.04  -  2028.03 

  Direct: 35,500,000 (YEN)  Overheads: 46,150,000 (YEN)  Total: 10,650,000 (YEN)

• Grant-in-Aid for Scientific Research(A)

  Project Year: 2021.04  -  2024.03 

  Direct: 32,400,000 (YEN)  Overheads: 42,120,000 (YEN)  Total: 9,720,000 (YEN)

• Grant-in-Aid for Scientific Research(A)

  Project Year: 2021.04  -  2024.03 

  Direct: 32,400,000 (YEN)  Overheads: 42,120,000 (YEN)  Total: 9,720,000 (YEN)

• Grant-in-Aid for Scientific Research(A)

  Project Year: 2021.04  -  2024.03 

  Direct: 32,400,000 (YEN)  Overheads: 42,120,000 (YEN)  Total: 9,720,000 (YEN)

display all >>

SDGs 【 display / non-display 】

SDGs 【 display / non-display 】

Social Activity 【 display / non-display 】

Social Activity 【 display / non-display 】

• 2025.04

  -

  2025.09

  

• 2023.10