Yagisawa Fumi

写真a

Title

Associate Professor

Researcher Number(JSPS Kakenhi)

70757658
To the head of this page.▲

Current Affiliation Organization 【 display / non-display 】

  • Concurrently   University of the Ryukyus   Graduate School of Engineering and Science   Chemistry, Biology and Marine Science   Associate Professor  

  • Duty   University of the Ryukyus   Research Facility Center   Associate Professor  

To the head of this page.▲

External Career 【 display / non-display 】

  • 2015.04
    　
    　

    University of the Ryukyus, Instrumental Research Center, Associate Professor  

To the head of this page.▲

Research Areas 【 display / non-display 】

  • Life Science / Cell biology

To the head of this page.▲

Published Papers 【 display / non-display 】

  • A fusion protein of polyphosphate kinase 1 (PPK1) and a Nudix hydrolase is involved in inorganic polyphosphate accumulation in the unicellular red alga Cyanidioschyzon merolae.

    Yagisawa F, Fujiwara T, Yamashita S, Hirooka S, Tamashiro K, Izumi J, Kanesaki Y, Onuma R, Misumi O, Nakamura S, Yoshikawa H, Kuroiwa H, Kuroiwa T, Miyagishima SY

    Plant molecular biology ( Springer Science and Business Media LLC )  115 ( 1 ) 9   2024.12 [ Peer Review Accepted ]

    Type of publication: Research paper (scientific journal)

  • Development of a rapamycin-inducible protein-knockdown system in the unicellular red alga Cyanidioschyzon merolae.

    Fujiwara T, Hirooka S, Yamashita S, Yagisawa F, Miyagishima SY

    Plant physiology ( Oxford University Press (OUP) )  196 ( 1 ) 77 - 94   2024.06 [ Peer Review Accepted ]

    Type of publication: Research paper (scientific journal)

    　View Summary

    Abstract An inducible protein-knockdown system is highly effective for investigating the functions of proteins and mechanisms essential for the survival and growth of organisms. However, this technique is not available in photosynthetic eukaryotes. The unicellular red alga Cyanidioschyzon merolae possesses a very simple cellular and genomic architecture and is genetically tractable but lacks RNA interference machinery. In this study, we developed a protein-knockdown system in this alga. The constitutive system utilizes the destabilizing activity of the FK506-binding protein 12 (FKBP12)-rapamycin-binding (FRB) domain of human target of rapamycin kinase or its derivatives to knock down target proteins. In the inducible system, rapamycin treatment induces the heterodimerization of the human FRB domain fused to the target proteins with the human FKBP fused to S-phase kinase-associated protein 1 or Cullin 1, subunits of the SCF E3 ubiquitin ligase. This results in the rapid degradation of the target proteins through the ubiquitin-proteasome pathway. With this system, we successfully degraded endogenous essential proteins such as the chloroplast division protein dynamin-related protein 5B and E2 transcription factor, a regulator of the G1/S transition, within 2 to 3 h after rapamycin administration, enabling the assessment of resulting phenotypes. This rapamycin-inducible protein-knockdown system contributes to the functional analysis of genes whose disruption leads to lethality.

  • Complete mitochondrial and chloroplast DNA sequences of the freshwater green microalga Medakamo hakoo.

    Takusagawa M, Misumi O, Nozaki H, Kato S, Maruyama S, Tsujimoto-Inui Y, Yagisawa F, Ohnuma M, Kuroiwa H, Kuroiwa T, Matsunaga S

    Genes & genetic systems ( 日本遺伝学会 )  98 ( 6 ) 353 - 360   2024.02

    Type of publication: Research paper (scientific journal)

    　View Summary

    <p>We report the complete organellar genome sequences of an ultrasmall green alga, <i>Medakamo hakoo</i> strain M-hakoo 311, which has the smallest known nuclear genome in freshwater green algae. <i>Medakamo hakoo</i> has 90.8-kb chloroplast and 36.5-kb mitochondrial genomes containing 80 and 33 putative protein-coding genes, respectively. The mitochondrial genome is the smallest in the Trebouxiophyceae algae studied so far. The GC content of the nuclear genome is 73%, but those of chloroplast and mitochondrial genomes are 41% and 35%, respectively. Codon usages in the organellar genomes have a different tendency from that in the nuclear genome. The organellar genomes have unique characteristics, such as the biased encoding of mitochondrial genes on a single strand and the absence of operon structures in chloroplast ribosomal genes. <i>Medakamo hakoo</i> will be helpful for understanding the evolution of the organellar genome and the regulation of gene expression in chloroplasts and mitochondria.</p>

  • Complete mitochondrial and chloroplast DNA sequences of the freshwater green microalga Medakamo hakoo.

    Takusagawa, M., Misumi, O., Nozaki, H., Kato, S., Maruyama, S., Tsujimoto-Inui, Y., Yagisawa, F., Ohnuma, M., Kuroiwa, H., Kuroiwa, T. and Matsunaga, S.

    Genes & Genetic Systems     2024.01 [ Peer Review Accepted ]

    Type of publication: Research paper (scientific journal)

    • Access this article
      DOI Open Access

  • Genomic analysis of an ultrasmall freshwater green alga, Medakamo hakoo.

    Kato S, Misumi O, Maruyama S, Nozaki H, Tsujimoto-Inui Y, Takusagawa M, Suzuki S, Kuwata K, Noda S, Ito N, Okabe Y, Sakamoto T, Yagisawa F, Matsunaga TM, Matsubayashi Y, Yamaguchi H, Kawachi M, Kuroiwa H, Kuroiwa T, Matsunaga S

    Communications biology ( Springer Science and Business Media LLC )  6 ( 1 ) 89   2023.01 [ Peer Review Accepted ]

    Type of publication: Research paper (scientific journal)

    　View Summary

    Abstract Ultrasmall algae have attracted the attention of biologists investigating the basic mechanisms underlying living systems. Their potential as effective organisms for producing useful substances is also of interest in bioindustry. Although genomic information is indispensable for elucidating metabolism and promoting molecular breeding, many ultrasmall algae remain genetically uncharacterized. Here, we present the nuclear genome sequence of an ultrasmall green alga of freshwater habitats, Medakamo hakoo. Evolutionary analyses suggest that this species belongs to a new genus within the class Trebouxiophyceae. Sequencing analyses revealed that its genome, comprising 15.8 Mbp and 7629 genes, is among the smallest known genomes in the Viridiplantae. Its genome has relatively few genes associated with genetic information processing, basal transcription factors, and RNA transport. Comparative analyses revealed that 1263 orthogroups were shared among 15 ultrasmall algae from distinct phylogenetic lineages. The shared gene sets will enable identification of genes essential for algal metabolism and cellular functions.

display all >>

To the head of this page.▲

Books 【 display / non-display 】

  • Cyanidioschyzon merolae: A New Model Eukaryote for Cell and Organelle Biology

    Yagisawa F, Imoto Y, Fujiwara T, Miyagishima S ( Part: Multiple Authorship ,  16. Single-Membrane-Bound organelles: Division and Inheritance )

    Springer  2018.02

To the head of this page.▲

Other Papers 【 display / non-display 】

To the head of this page.▲

Grant-in-Aid for Scientific Research 【 display / non-display 】

  • Grant-in-Aid for Scientific Research(B)

    Project Year: 2022.04  -  2025.03 

  • Grant-in-Aid for Scientific Research(C)

    Project Year: 2022.04  -  2025.03 

    Direct: 3,200,000 (YEN)  Overheads: 960,000 (YEN) 

  • Grant-in-Aid for Scientific Research(C)

    Project Year: 2022.04  -  2025.03 

    Direct: 3,200,000 (YEN)  Overheads: 4,160,000 (YEN)  Total: 960,000 (YEN)

  • Grant-in-Aid for Scientific Research(C)

    Project Year: 2022.04  -  2025.03 

    Direct: 3,200,000 (YEN)  Overheads: 4,160,000 (YEN)  Total: 960,000 (YEN)

  • Elucidating the basis of plant cells based on the organelle division mechanism of microalgae

    Grant-in-Aid for Scientific Research(B)

    Project Year: 2022.04  -  2025.03 

    Direct: 13,600,000 (YEN)  Overheads: 17,680,000 (YEN)  Total: 4,080,000 (YEN)

display all >>

To the head of this page.▲