八木沢 芙美 (ヤギサワ フミ)

Yagisawa Fumi

写真a

職名

准教授

科研費研究者番号

70757658

現在の所属組織 【 表示 / 非表示

  • 併任   琉球大学   理工学研究科   海洋自然科学専攻   准教授  

  • 専任   琉球大学   研究基盤統括センター   准教授  

取得学位 【 表示 / 非表示

  • 東京大学 -  博士(理学)  理学

  • 東京大学 -  修士(理学)  理学

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  • 2015年04月
    -
    継続中

      琉球大学 機器分析支援センター 准教授  

研究分野 【 表示 / 非表示

  • ライフサイエンス / 細胞生物学

論文 【 表示 / 非表示

  • Development of a rapamycin-inducible protein-knockdown system in the unicellular red alga Cyanidioschyzon merolae.

    Fujiwara T, Hirooka S, Yamashita S, Yagisawa F, Miyagishima SY

    Plant physiology ( Oxford University Press (OUP) )    2024年06月 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

     概要を見る

    Abstract An inducible protein-knockdown system is highly effective for investigating the functions of proteins and mechanisms essential for the survival and growth of organisms. However, this technique is not available in photosynthetic eukaryotes. The unicellular red alga Cyanidioschyzon merolae possesses a very simple cellular and genomic architecture and is genetically tractable but lacks RNA interference machinery. In this study, we developed a protein-knockdown system in this alga. The constitutive system utilizes the destabilizing activity of the FK506-binding protein 12 (FKBP12)-rapamycin-binding (FRB) domain of human target of rapamycin kinase or its derivatives to knock down target proteins. In the inducible system, rapamycin treatment induces the heterodimerization of the human FRB domain fused to the target proteins with the human FKBP fused to S-phase kinase-associated protein 1 or Cullin 1, subunits of the SCF E3 ubiquitin ligase. This results in the rapid degradation of the target proteins through the ubiquitin-proteasome pathway. With this system, we successfully degraded endogenous essential proteins such as the chloroplast division protein dynamin-related protein 5B and E2 transcription factor, a regulator of the G1/S transition, within 2 to 3 h after rapamycin administration, enabling the assessment of resulting phenotypes. This rapamycin-inducible protein-knockdown system contributes to the functional analysis of genes whose disruption leads to lethality.

  • Complete mitochondrial and chloroplast DNA sequences of the freshwater green microalga Medakamo hakoo.

    Takusagawa, M., Misumi, O., Nozaki, H., Kato, S., Maruyama, S., Tsujimoto-Inui, Y., Yagisawa, F., Ohnuma, M., Kuroiwa, H., Kuroiwa, T. and Matsunaga, S.

    Genes & Genetic Systems     2024年01月 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

  • Genomic analysis of an ultrasmall freshwater green alga, Medakamo hakoo.

    Kato S, Misumi O, Maruyama S, Nozaki H, Tsujimoto-Inui Y, Takusagawa M, Suzuki S, Kuwata K, Noda S, Ito N, Okabe Y, Sakamoto T, Yagisawa F, Matsunaga TM, Matsubayashi Y, Yamaguchi H, Kawachi M, Kuroiwa H, Kuroiwa T, Matsunaga S

    Communications biology ( Springer Science and Business Media LLC )  6 ( 1 ) 89   2023年01月 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

     概要を見る

    Abstract Ultrasmall algae have attracted the attention of biologists investigating the basic mechanisms underlying living systems. Their potential as effective organisms for producing useful substances is also of interest in bioindustry. Although genomic information is indispensable for elucidating metabolism and promoting molecular breeding, many ultrasmall algae remain genetically uncharacterized. Here, we present the nuclear genome sequence of an ultrasmall green alga of freshwater habitats, Medakamo hakoo. Evolutionary analyses suggest that this species belongs to a new genus within the class Trebouxiophyceae. Sequencing analyses revealed that its genome, comprising 15.8 Mbp and 7629 genes, is among the smallest known genomes in the Viridiplantae. Its genome has relatively few genes associated with genetic information processing, basal transcription factors, and RNA transport. Comparative analyses revealed that 1263 orthogroups were shared among 15 ultrasmall algae from distinct phylogenetic lineages. The shared gene sets will enable identification of genes essential for algal metabolism and cellular functions.

  • Complete Mitochondrial and Plastid DNA Sequences of the Freshwater Green Microalga Medakamo hakoo

    Mari Takusagawa, Shoichi Kato, Sachihiro Matsunaga, Shinichiro Maruyama, Yayoi Tsujimoto-Inui, Hisayoshi Nozaki, Fumi Yagisawa, Mio Ohnuma, Haruko Kuroiwa, Tsuneyoshi Kuroiwa, Osami Misumi

    bioRxiv ( Cold Spring Harbor Laboratory )    2021年07月

    掲載種別: 研究論文(学術雑誌)

     概要を見る

    <jats:p>Here we report the complete organellar genome sequences of <jats:italic>Medakamo hakoo</jats:italic>, a green alga identified in freshwater in Japan. It has 90.8-kb plastid and 36.5-kb mitochondrial genomes containing 80 and 33 putative protein coding genes, respectively, representing the smallest organellar genome among currently known core Trebouxiophyceae.</jats:p>

  • Smooth Loop-Like Mitochondrial Nucleus in the Primitive Red Alga <i>Cyanidioschyzon merolae</i> Revealed by Drying Treatment

    Kuroiwa Tsuneyoshi, Yagisawa Fumi, Fujiwara Takayuki, Misumi Osami, Nagata Noriko, Imoto Yuuta, Yoshida Yamato, Mogi Yuko, Miyagishima Shin-ya, Kuroiwa Haruko

    CYTOLOGIA ( 公益財団法人 日本メンデル協会 )  86 ( 1 ) 89 - 96   2021年03月 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

     概要を見る

    <p>It is thought that mitochondria were generated by the symbiosis of autonomous α-proteobacteria and a eukaryote-like organism derived from an archaeon of the species <i>Sulfolobus</i>. Soon after the symbiosis, most of the genome of the α-proteobacterium, which was required for autonomy, was lost. Many genes were transferred into the host genome. However, a small amount of DNA—the mitochondrial genome (mt-genome, mtDNA)—remained in the symbiotic organelle. The primitive eukaryotic cells increased the mtDNA copy number and formed a mitochondrial nucleus (mt-nucleus). The primitive unicellular eukaryote evolved into organisms with one mitochondrion containing multiple mtDNA copies per cell, and organisms with multiple mitochondria with a small number of mtDNA copies in each cell. There have been many studies on the mitochondria and mt-genomes of amoeba, plants, and animals which contain many mitochondria per cell, but only a few studies have reported morphological characteristics of the mitochondria and their genomes in primitive unicellular organisms that have only a single mitochondrion per cell. Here, we show that centrally located mt-nuclei in the primitive red alga <i>Cyanidioschyzon merolae</i> form smooth rings following the application of a drying method that produces slight cell swelling. We discuss regulatory mechanisms for genome function in endosymbiotic organelles on the basis of the differences between the copy number of mtDNA in smooth-ring shaped mt-nuclei and plastid DNA in bead-shaped plastid nuclei.</p>

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著書 【 表示 / 非表示

  • Cyanidioschyzon merolae: A New Model Eukaryote for Cell and Organelle Biology

    Yagisawa F, Imoto Y, Fujiwara T, Miyagishima S ( 担当: 共著 , 担当範囲: 16. Single-Membrane-Bound organelles: Division and Inheritance )

    Springer  2018年02月 ( 担当ページ: p.235-249 )

科研費獲得情報 【 表示 / 非表示

  • 微細藻類のオルガネラ分裂機構を基盤に植物細胞の基を解く

    基盤研究(B)

    課題番号: 22H02657

    研究期間: 2022年04月  -  2025年03月 

    代表者: 黒岩常祥  研究分担者: 永田典子

  • 真核光合成生物における貯蔵物質代謝を介した生存戦略の解明

    基盤研究(C)

    課題番号: 22K06299

    研究期間: 2022年04月  -  2025年03月 

    直接経費: 3,200,000(円)  間接経費: 960,000(円) 

     概要を見る

    物は無機および有機養分の枯渇に備えてこれらを貯蔵し、必要時に利用する。このような貯蔵物質の合成と分解は、生物の持つ最も基本的な生存戦略の一つであるが、この戦略を支えるしくみはよく分かっていない。たとえば、貯蔵物質の合成と分解の切り替えは直近の代謝経路だけでなく、他の代謝経路群の変動も必要とすることが予想されるが、このような包括的な変動はほとんど議論されていない。本研究では、ゲノムサイズが小さく遺伝子組換え技術が確立された単細胞藻類をモデルに、貯蔵物質の合成・分解の切り替えに伴う細胞全体の代謝変動などを解析し、光合成生物における貯蔵物質代謝を介した生存戦略を明らかにする。

  • 真核光合成生物における貯蔵物質代謝を介した生存戦略の解明

    基盤研究(C)

    課題番号: 22K06299

    研究期間: 2022年04月  -  2025年03月 

    代表者: 八木沢 芙美 

    直接経費: 3,200,000(円)  間接経費: 4,160,000(円)  金額合計: 960,000(円)

  • オルガネラ分裂/増殖機構を基盤にした真核植物細胞の基のゲノム形態学的解明

    基盤研究(B)

    課題番号: 19H03260

    研究期間: 2019年04月  -  2022年03月 

    代表者: 黒岩 常祥  研究分担者: 永田 典子, 八木沢 芙美

    直接経費: 13,100,000(円)  間接経費: 3,930,000(円)  金額合計: 17,030,000(円)

  • ミトコンドリアとリソソームの物理的相互作用の分子機構と生理的意義の解明

    基盤研究(C)

    課題番号: 19K06742

    研究期間: 2019年04月  -  2022年03月 

    代表者: 八木沢 芙美 

    直接経費: 3,300,000(円)  間接経費: 4,290,000(円)  金額合計: 990,000(円)

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