Toyama Hirohide

写真a

Title

Professor

Researcher Number(JSPS Kakenhi)

60240884
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Current Affiliation Organization 【 display / non-display 】

  • Duty   University of the Ryukyus   Faculty of Agriculture   Professor  

  • Concurrently   University of the Ryukyus   Graduate School of Agriculture   Professor  

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University 【 display / non-display 】

  • 1982.04
    -
    1986.03

    Kyoto University   Faculty of Engineering   Department of Industrial Chemistry   Graduated

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Graduate School 【 display / non-display 】

  • 1986.04
    -
    1988.03

    Kyoto University  Graduate School, Division of Engineering  Master's Course  Completed

  • 1988.04
    -
    1992.03

    Kyoto University  Graduate School, Division of Agriculture  Doctor's Course (second term)  Completed

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Academic degree 【 display / non-display 】

  • Kyoto University -  PhD

  • Kyoto University -  Master of Engineering

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External Career 【 display / non-display 】

  • 1991.09
    -
    2001.10

    Yamaguchi University, Faculty of Agriculture, Biosclence and Biotechnology ,Research Assistants  

  • 2001.11
    -
    2006.03

    Yamaguchi University, Faculty of Agriculture,  

  • 2006.04
    -
    2007.03

    Yamaguchi University Graduate School of Medicine, Department of Applied Molecular Bioscience, Associate Professor  

  • 2007.04
    -
    2009.03

    University of the Ryukyus, Faculty of Agriculture, Biosclence and Biotechnology, Applied Biochemistry, Professor  

  • 2009.04
    　
    　

    University of the Ryukyus, Faculty of Agriculture, Department of Subtropical Biochemistry and Biotechnology, Fermentation and Life Science, Professor  

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Affiliated academic organizations 【 display / non-display 】

  • 1989.06
    -
    Now
     

    Japan Society for Bioscience, Biotechnology, and Agrochemistry 

  • 1986.11
    -
    Now
     

    The Japanese Biochemical Society 

  • 2001.03
    -
    Now
     

    The Vitamin Society of Japan 

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Research Interests 【 display / non-display 】

  • pyrroloquinoline quinone

  • Aspergillus luchuensis

  • Awamori yeast

  • 酢酸菌

  • awamori

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Research Areas 【 display / non-display 】

  • Aspergillus luchuensis, Acetic acid bacteria, Awamori yeast

  • pyrroloquinoline quinone

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Published Papers 【 display / non-display 】

  • Characterization of 3 phylogenetically distinct membrane-bound d-gluconate dehydrogenases of Gluconobacter spp. and their biotechnological application for efficient 2-keto-d-gluconate production.

    Kataoka N, Saichana N, Matsutani M, Toyama H, Matsushita K, Yakushi T

    Bioscience, biotechnology, and biochemistry ( Bioscience, Biotechnology and Biochemistry )  86 ( 5 ) 681 - 690   2022.04 [ Peer Review Accepted ]

    Type of publication: Research paper (scientific journal)

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    We identified a novel flavoprotein-cytochrome c complex d-gluconate dehydrogenase (GADH) encoded by gndXYZ of Gluconobacter oxydans NBRC 3293, which is phylogenetically distinct from previously reported GADHs encoded by gndFGH and gndSLC of Gluconobacter spp. To analyze the biochemical properties of respective GADHs, Gluconobacter japonicus NBRC 3271 mutant strain lacking membranous d-gluconate dehydrogenase activity was constructed. All GADHs (GndFGH, GndSLC, and GndXYZ) were successfully overexpressed in the constructed strain. The optimal pH and KM value at that pH of GndFGH, GndSLC, and GndXYZ were 5, 6, and 4, and 8.82 ± 1.15, 22.9 ± 5.0, and 11.3 ± 1.5 m m, respectively. When the mutants overexpressing respective GADHs were cultured in d-glucose-containing medium, all of them produced 2-keto-d-gluconate, revealing that GndXYZ converts d-gluconate to 2-keto-d-gluconate as well as other GADHs. Among the recombinants, the gndXYZ-overexpressing strain accumulated the highest level of 2-keto-d-gluconate, suggesting its potential for 2-keto-d-gluconate production.

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  • Oxidative Fermentation of Acetic Acid Bacteria and Its Products.

    He Y, Xie Z, Zhang H, Liebl W, Toyama H, Chen F

    Frontiers in microbiology ( Frontiers in Microbiology )  13   879246   2022 [ Peer Review Accepted ]

    Type of publication: Research paper (scientific journal)

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  • Draft Genome Sequence of Saccharomyces cerevisiae Strain Awamori Number 101, Commonly Used to Make Awamori, a Traditional Spirit, in Okinawa, Japan.

    Tsukahara M, Ise K, Nezuo M, Azuma H, Akao T, Toyama H

    Microbiology resource announcements ( Microbiology Resource Announcements )  10 ( 25 ) e0141420   2021.06 [ Peer Review Accepted ]

    Type of publication: Research paper (scientific journal)

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    We report here the draft genome sequence for Saccharomyces cerevisiae strain Awamori number 101, an industrial strain used for producing awamori, a distilled alcohol beverage. It was constructed by assembling the short reads obtained by next-generation sequencing. The 315 contigs constitute an 11.5-Mbp genome sequence encoding 6,185 predicted proteins.

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  • Development of an itraconazole resistance gene as a dominant selectable marker for transformation in Aspergillus oryzae and Aspergillus luchuensis.

    Tokashiki J, Toyama H, Mizutani O

    Bioscience, biotechnology, and biochemistry ( Bioscience, Biotechnology and Biochemistry )  85 ( 3 ) 722 - 727   2021.02 [ Peer Review Accepted ]

    Type of publication: Research paper (scientific journal)

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    There are only a few combinations of antifungal drugs with known resistance marker genes in the Aspergillus species; therefore, the transformation of their wild-type strains is limited. In this study, to develop the novel dominant selectable marker for itraconazole, a fungal cell membrane synthesis inhibitor, we focused on Aspergillus luchuensis cyp51A (Alcyp51A), which encodes a 14-α-sterol demethylase related to the steroid synthesis pathway. We found that the G52R mutation in AlCyp51A and the replacement of the native promoter with a high-expression promoter contributed to itraconazole resistance in Aspergillus oryzae, designated as itraconazole resistant gene (itrA). The random integration in the A. luchuensis genome of the itrA marker cassette gene also allowed for transformation using itraconazole. Therefore, we succeed in developing a novel itraconazole resistance marker as a dominant selectable marker for transformation in A. oryzae and A. luchuensis.

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  • Characterization of thermotolerant acetic acid bacteria isolated from various plant beverages in Thailand

    Charee P.

    Applied Food Biotechnology ( Applied Food Biotechnology )  7 ( 2 ) 61 - 72   2020.03 [ Peer Review Accepted ]

    Type of publication: Research paper (scientific journal)

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Books 【 display / non-display 】

  • Industrial Biotechnology of Vitamins, Biopigments, and Antioxidants

    Hirohide Toyama ( Part: Multiple Authorship ,  13 Pyrroloquinoline Quinone (PQQ) )

    Wiley-VCHVerlag GmbH & Co. KGaA  2016.07

  • Acetic acid bacteria: Ecology and physiology

    Matsushita K., Toyama H., Tonouchi N., Okamoto-Kainuma A. ( Part: Multiple Authorship )

    Acetic Acid Bacteria: Ecology and Physiology  2016.06

  • Acetic acid bacteria: Ecology and physiology

    Shinjoh M., Toyama H. ( Part: Multiple Authorship ,  Chapter 15 Industrial Application of Acetic Acid Bacteria (Vitamin C and Others) )

    Acetic Acid Bacteria: Ecology and Physiology  2016.06

  • Distilled Spirits -Future Challenges, New Solution

    Toyama H., Watanabe T., Tsukahara M. ( Part: Multiple Authorship ,  Chapter 10 Introduction to Awamori and microbes involved in its production )

    Context Products Ltd  2015.03

  • Modern Multidisciplinary Applied Microbiology

    J. Trcek, H. Toyama, J. Czuba, A. Misiewicz, K. Matsushita ( Part: Multiple Authorship )

    Wiley-VCH Verlag GmbH & Co. KGaA  2006.01

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Other Papers 【 display / non-display 】

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Presentations 【 display / non-display 】

  • Awamori, Traditional Distilled Liquor in Okinawa

    Toyama H., Mizutani O., Tsukahara M.

    2018 Korean Traditional Science forum and Exhibition  (EUlLim Hall, Gwachon National Science Museum)  2018.11  -  2018.11 

  • 麹菌 ligD 遺伝子がゲノム編集に起因する大規模欠失変異に与える影響

    上元 優, 林 梨咲, 渡嘉敷直杏, 荒添貴之, 山田 修, 外山博英, 水谷 治

    第３回ゲノム編集学会  (広島)  2018.06  -  2018.06 

  • Yeast strains isolated from Sri Lanka show higher tolerance to inhibitors derived from hydrolysis of lignocellulosic materials

    Maduka Subodinee Ahangangoda Arachchige，水谷 治, 外山博英

    日本農芸化学会 2018 年度大会  (名古屋)  2018.03  -  2018.03 

  • Microbes involved in brewing of Awamori, traditional distilled liquor in Okinawa

    Toyama H., Mizutani O., Tsukahara M.

    The 7th International Conference on Fermentation Technology for Value Added Agrocultural Products and The 12th Asian Biohydrogen & Biorefinery Symposium with Joint Session from JSPS-NRCT New Core to Core Program A. Advanced Research Network  (Pullman Khon Kaen Raja Orchid Hotel, Khon Kaen, Thailand )  2017.07  -  2017.07 

  • Involvement of ubiI gene in PQQ biosynthesis in Methylopila sp. Yamaguchi

    Ishikawa H., Toyama H.

    The Fifth International Conference on Cofactors & Active Enzyme Molecule 2016  (Kurobe Unazuki International Hall, Toyama, Japan)  2016.09  -  2016.09 

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