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Published Papers 【 display / non-display 】

Published Papers 【 display / non-display 】

• Characterization of 3 phylogenetically distinct membrane-bound d-gluconate dehydrogenases of Gluconobacter spp. and their biotechnological application for efficient 2-keto-d-gluconate production.

  Kataoka N, Saichana N, Matsutani M, Toyama H, Matsushita K, Yakushi T

  Bioscience, biotechnology, and biochemistry ( Bioscience, Biotechnology and Biochemistry )  86 ( 5 ) 681 - 690   2022.04 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  We identified a novel flavoprotein-cytochrome c complex d-gluconate dehydrogenase (GADH) encoded by gndXYZ of Gluconobacter oxydans NBRC 3293, which is phylogenetically distinct from previously reported GADHs encoded by gndFGH and gndSLC of Gluconobacter spp. To analyze the biochemical properties of respective GADHs, Gluconobacter japonicus NBRC 3271 mutant strain lacking membranous d-gluconate dehydrogenase activity was constructed. All GADHs (GndFGH, GndSLC, and GndXYZ) were successfully overexpressed in the constructed strain. The optimal pH and KM value at that pH of GndFGH, GndSLC, and GndXYZ were 5, 6, and 4, and 8.82 ± 1.15, 22.9 ± 5.0, and 11.3 ± 1.5 m m, respectively. When the mutants overexpressing respective GADHs were cultured in d-glucose-containing medium, all of them produced 2-keto-d-gluconate, revealing that GndXYZ converts d-gluconate to 2-keto-d-gluconate as well as other GADHs. Among the recombinants, the gndXYZ-overexpressing strain accumulated the highest level of 2-keto-d-gluconate, suggesting its potential for 2-keto-d-gluconate production.

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• Inhibitory Effect of Water Soluble Fraction of Monascus-Fermented Rice on Lipid Accumulation in 3T3 L1 Adipocyte

  Wanninaika I.P.

  Current Applied Science and Technology ( Current Applied Science and Technology )  22 ( 6 )   2022.02 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Oxidative Fermentation of Acetic Acid Bacteria and Its Products.

  He Y, Xie Z, Zhang H, Liebl W, Toyama H, Chen F

  Frontiers in microbiology ( Frontiers in Microbiology )  13   879246   2022 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Phylogenetic analysis of <i>Aspergillus luchuensis</i> strains based on whole genome analysis

  TSUKAHARA Masatoshi, AZUMA Haruna, MIZUTANI Osamu, YAMADA Osamu, TOYAMA Hirohide

  JOURNAL OF THE BREWING SOCIETY OF JAPAN ( Brewing Society of Japan )  117 ( 6 ) 413 - 421   2022 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  <p>Artificial selection of microorganisms in the fermentation industry may promote differentiation into domestication. Black-koji mold utilized for awamori brewing appears to be optimized for alcohol fermentation and safe manufacturing with a high enzymic activity, citric acid production, and null toxin productivity. Recently, the diverse strains of black-koji mold were proved to be identical in several genetic codings and were defined as a novel species, <i>Aspergillus luchuensis</i>. In this study, to determine the genetic basis for the diversity of <i>A. luchuensis</i>, a genome comparison of 41 strains including <i>A. luchuensis</i> and related species was conducted. Whole genome sequencing was performed by the next-generation sequencer. Each obtained short reads data was mapped to the <i>A. luchuensis</i> RIB2604(NBRC4314) genome as reference. The detected single nucleotide variants were retained for subsequent phylogenic analysis. The results indicated the differentiations accounting for diverse traits occurred in <i>A. luchuensis</i>, and that <i>A. luchuensis</i> is divided into two groups.</p>

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• Draft Genome Sequence of Saccharomyces cerevisiae Strain Awamori Number 101, Commonly Used to Make Awamori, a Traditional Spirit, in Okinawa, Japan.

  Tsukahara M, Ise K, Nezuo M, Azuma H, Akao T, Toyama H

  Microbiology resource announcements ( Microbiology Resource Announcements )  10 ( 25 ) e0141420   2021.06 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  We report here the draft genome sequence for Saccharomyces cerevisiae strain Awamori number 101, an industrial strain used for producing awamori, a distilled alcohol beverage. It was constructed by assembling the short reads obtained by next-generation sequencing. The 315 contigs constitute an 11.5-Mbp genome sequence encoding 6,185 predicted proteins.

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Books 【 display / non-display 】

• Industrial Biotechnology of Vitamins, Biopigments, and Antioxidants

  Hirohide Toyama ( Part: Multiple Authorship ,  13 Pyrroloquinoline Quinone (PQQ) )

  Wiley-VCHVerlag GmbH & Co. KGaA  2016.07

• Acetic acid bacteria: Ecology and physiology

  Matsushita K., Toyama H., Tonouchi N., Okamoto-Kainuma A. ( Part: Multiple Authorship )

  Acetic Acid Bacteria: Ecology and Physiology  2016.06

• Acetic acid bacteria: Ecology and physiology

  Shinjoh M., Toyama H. ( Part: Multiple Authorship ,  Chapter 15 Industrial Application of Acetic Acid Bacteria (Vitamin C and Others) )

  Acetic Acid Bacteria: Ecology and Physiology  2016.06

• Distilled Spirits -Future Challenges, New Solution

  Toyama H., Watanabe T., Tsukahara M. ( Part: Multiple Authorship ,  Chapter 10 Introduction to Awamori and microbes involved in its production )

  Context Products Ltd  2015.03

• Modern Multidisciplinary Applied Microbiology

  J. Trcek, H. Toyama, J. Czuba, A. Misiewicz, K. Matsushita ( Part: Multiple Authorship )

  Wiley-VCH Verlag GmbH & Co. KGaA  2006.01

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• Development of awamori brewing technology with new flavor utilizing microbial functions

  Tsukahara Masatoshi, Yamada Osamu, Takagi Hiroshi, Toyama Hirohide

  Seibutsu-kogaku Kaishi ( The Society for Biotechnology, Japan )  101 ( 2 ) 68 - 73   2023.02

   

  DOI CiNii Research

• Effect of alternative oxidase on heat generation in koji making of Aspergillus luchuensis

  具志堅結香, 中村燎, 渡嘉敷直杏, 外山博英, 水谷治

  糸状菌分子生物学コンファレンス(Web)   21st   2022

   

  J-GLOBAL

• Construction of high-producing strains of nigeran in Aspergillus oryzae ΔAG-GAG strain

  阿部多恵, 與古田佳世, 阿部敬悦, 外山博英, 上地敬子, 水谷治

  糸状菌分子生物学コンファレンス(Web)   21st   2022

   

  J-GLOBAL

• Effects of PQQ and related compounds on osteoclast differentiation

  小林未侑, 外山博英, 橘信二郎

  日本農芸化学会大会講演要旨集(Web)   2020   2020

   

  J-GLOBAL

• Development of a new marker for Aspergillus transformation using Itraconazole resistance

  渡嘉敷直杏, 水谷治, 水谷治, 外山博英, 外山博英

  日本農芸化学会大会講演要旨集(Web)   2020   2020

   

  J-GLOBAL

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Presentations 【 display / non-display 】

Presentations 【 display / non-display 】

• Awamori, Traditional Distilled Liquor in Okinawa

  Toyama H., Mizutani O., Tsukahara M.

  2018 Korean Traditional Science forum and Exhibition  (EUlLim Hall, Gwachon National Science Museum)  2018.11  -  2018.11 

• 麹菌 ligD 遺伝子がゲノム編集に起因する大規模欠失変異に与える影響

  上元 優, 林 梨咲, 渡嘉敷直杏, 荒添貴之, 山田 修, 外山博英, 水谷 治

  第３回ゲノム編集学会  (広島)  2018.06  -  2018.06 

• Yeast strains isolated from Sri Lanka show higher tolerance to inhibitors derived from hydrolysis of lignocellulosic materials

  Maduka Subodinee Ahangangoda Arachchige，水谷 治, 外山博英

  日本農芸化学会 2018 年度大会  (名古屋)  2018.03  -  2018.03 

• Microbes involved in brewing of Awamori, traditional distilled liquor in Okinawa

  Toyama H., Mizutani O., Tsukahara M.

  The 7th International Conference on Fermentation Technology for Value Added Agrocultural Products and The 12th Asian Biohydrogen & Biorefinery Symposium with Joint Session from JSPS-NRCT New Core to Core Program A. Advanced Research Network  (Pullman Khon Kaen Raja Orchid Hotel, Khon Kaen, Thailand )  2017.07  -  2017.07 

• Involvement of ubiI gene in PQQ biosynthesis in Methylopila sp. Yamaguchi

  Ishikawa H., Toyama H.

  The Fifth International Conference on Cofactors & Active Enzyme Molecule 2016  (Kurobe Unazuki International Hall, Toyama, Japan)  2016.09  -  2016.09 

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• Grant-in-Aid for Scientific Research(C)

  Project Year: 2007  -  2007 

  Direct: 1,100,000 (YEN)  Overheads: 330,000 (YEN)  Total: 1,430,000 (YEN)

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2006  -  2007 

  Direct: 2,400,000 (YEN)  Total: 2,400,000 (YEN)