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Published Papers 【 display / non-display 】

Published Papers 【 display / non-display 】

• Effects of lactic acid bacteria on 4-vinylguaiacol production during the Awamori brewing process

  TSUKAHARA Masatoshi, TAMAKI Yasutomo, AZUMA Haruna, KUGAI Tatsumoto, TOYAMA Hirohide

  JOURNAL OF THE BREWING SOCIETY OF JAPAN ( Brewing Society of Japan )  118 ( 3 ) 185 - 192   2023.03 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  <p>Awamori, a traditional Okinawan distilled liquor, has the characteristic that its flavor improves after aging. Vanillin is one of the aged aroma components of Awamori which is converted from 4-vinyl guaiacol(4-VG) derived from ferulic acid in fermenting mash. In this report, we analyzed the effects of lactic acid bacteria in Awamori mash on the conversion of ferulic acid to 4-VG. Lactic acid bacteria were isolated from Awamori mash of breweries, and the genera <i>Lactobacillus</i>, <i>Lactococcus</i>, <i>Leuconostoc</i>, and <i>Propionibacterium</i> were found. Several strains, such as <i>Lactococcus lactis</i>, exhibited a conversion activity from ferulic acid to 4-VG, and <i>Lactiplantibacillus plantarum</i> converted ferulic acid to dihydroferulic acid. It was also confirmed that these types of strains have a similar activity, respectively. A small-scale fermentation test with added <i>L. lactis</i> led to an increase in 4-VG, and the addition of <i>L. plantarum</i> led to its decrease, while both bacteria were unaffected in alcohol production. The effects of these lactic acid bacteria were also confirmed in Awamori after distillation. These results suggest that lactic acid bacteria in mash may affect the 4-VG concentration during the fermentation process of Awamori, and the addition of lactic acid bacteria may be a new Awamori brewing technique to increase the 4-VG concentration.</p>

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• Characterization of 3 phylogenetically distinct membrane-bound d-gluconate dehydrogenases of Gluconobacter spp. and their biotechnological application for efficient 2-keto-d-gluconate production.

  Kataoka N, Saichana N, Matsutani M, Toyama H, Matsushita K, Yakushi T

  Bioscience, biotechnology, and biochemistry ( Bioscience, Biotechnology and Biochemistry )  86 ( 5 ) 681 - 690   2022.04 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  We identified a novel flavoprotein-cytochrome c complex d-gluconate dehydrogenase (GADH) encoded by gndXYZ of Gluconobacter oxydans NBRC 3293, which is phylogenetically distinct from previously reported GADHs encoded by gndFGH and gndSLC of Gluconobacter spp. To analyze the biochemical properties of respective GADHs, Gluconobacter japonicus NBRC 3271 mutant strain lacking membranous d-gluconate dehydrogenase activity was constructed. All GADHs (GndFGH, GndSLC, and GndXYZ) were successfully overexpressed in the constructed strain. The optimal pH and KM value at that pH of GndFGH, GndSLC, and GndXYZ were 5, 6, and 4, and 8.82 ± 1.15, 22.9 ± 5.0, and 11.3 ± 1.5 m m, respectively. When the mutants overexpressing respective GADHs were cultured in d-glucose-containing medium, all of them produced 2-keto-d-gluconate, revealing that GndXYZ converts d-gluconate to 2-keto-d-gluconate as well as other GADHs. Among the recombinants, the gndXYZ-overexpressing strain accumulated the highest level of 2-keto-d-gluconate, suggesting its potential for 2-keto-d-gluconate production.

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• Inhibitory Effect of Water Soluble Fraction of Monascus-Fermented Rice on Lipid Accumulation in 3T3 L1 Adipocyte

  Indika Pradeep Wanninaika, Hirohide Toyama, Shinjiro Thachibana, Perumpuli Arachchige Buddhika Niroshie Perumpuli, Chamari Hettiarachchi and Migelhewa Nidarsha Kaumal

  Current Applied Science and Technology ( Current Applied Science and Technology )  22 ( 6 )   2022.02 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Oxidative Fermentation of Acetic Acid Bacteria and Its Products.

  He Y, Xie Z, Zhang H, Liebl W, Toyama H, Chen F

  Frontiers in microbiology ( Frontiers in Microbiology )  13   879246   2022 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Phylogenetic analysis of <i>Aspergillus luchuensis</i> strains based on whole genome analysis

  TSUKAHARA Masatoshi, AZUMA Haruna, MIZUTANI Osamu, YAMADA Osamu, TOYAMA Hirohide

  JOURNAL OF THE BREWING SOCIETY OF JAPAN ( Brewing Society of Japan )  117 ( 6 ) 413 - 421   2022 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  <p>Artificial selection of microorganisms in the fermentation industry may promote differentiation into domestication. Black-koji mold utilized for awamori brewing appears to be optimized for alcohol fermentation and safe manufacturing with a high enzymic activity, citric acid production, and null toxin productivity. Recently, the diverse strains of black-koji mold were proved to be identical in several genetic codings and were defined as a novel species, <i>Aspergillus luchuensis</i>. In this study, to determine the genetic basis for the diversity of <i>A. luchuensis</i>, a genome comparison of 41 strains including <i>A. luchuensis</i> and related species was conducted. Whole genome sequencing was performed by the next-generation sequencer. Each obtained short reads data was mapped to the <i>A. luchuensis</i> RIB2604(NBRC4314) genome as reference. The detected single nucleotide variants were retained for subsequent phylogenic analysis. The results indicated the differentiations accounting for diverse traits occurred in <i>A. luchuensis</i>, and that <i>A. luchuensis</i> is divided into two groups.</p>

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Books 【 display / non-display 】

Books 【 display / non-display 】

• Industrial Biotechnology of Vitamins, Biopigments, and Antioxidants

  Hirohide Toyama ( Part: Multiple Authorship ,  13 Pyrroloquinoline Quinone (PQQ) )

  Wiley-VCHVerlag GmbH & Co. KGaA  2016.07

• Acetic acid bacteria: Ecology and physiology

  Matsushita K., Toyama H., Tonouchi N., Okamoto-Kainuma A. ( Part: Multiple Authorship )

  Acetic Acid Bacteria: Ecology and Physiology  2016.06

• Acetic acid bacteria: Ecology and physiology

  Shinjoh M., Toyama H. ( Part: Multiple Authorship ,  Chapter 15 Industrial Application of Acetic Acid Bacteria (Vitamin C and Others) )

  Acetic Acid Bacteria: Ecology and Physiology  2016.06

• Distilled Spirits -Future Challenges, New Solution

  Toyama H., Watanabe T., Tsukahara M. ( Part: Multiple Authorship ,  Chapter 10 Introduction to Awamori and microbes involved in its production )

  Context Products Ltd  2015.03

• Modern Multidisciplinary Applied Microbiology

  J. Trcek, H. Toyama, J. Czuba, A. Misiewicz, K. Matsushita ( Part: Multiple Authorship )

  Wiley-VCH Verlag GmbH & Co. KGaA  2006.01

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Other Papers 【 display / non-display 】

Other Papers 【 display / non-display 】

• Development of awamori brewing technology with new flavor utilizing microbial functions

  Tsukahara Masatoshi, Yamada Osamu, Takagi Hiroshi, Toyama Hirohide

  Seibutsu-kogaku Kaishi ( The Society for Biotechnology, Japan )  101 ( 2 ) 68 - 73   2023.02

   

  DOI CiNii Research

• Effect of alternative oxidase on heat generation in koji making of Aspergillus luchuensis

  具志堅結香, 中村燎, 渡嘉敷直杏, 外山博英, 水谷治

  糸状菌分子生物学コンファレンス(Web)   21st   2022

   

  J-GLOBAL

• Construction of high-producing strains of nigeran in Aspergillus oryzae ΔAG-GAG strain

  阿部多恵, 與古田佳世, 阿部敬悦, 外山博英, 上地敬子, 水谷治

  糸状菌分子生物学コンファレンス(Web)   21st   2022

   

  J-GLOBAL

• Effects of PQQ and related compounds on osteoclast differentiation

  小林未侑, 外山博英, 橘信二郎

  日本農芸化学会大会講演要旨集(Web)   2020   2020

   

  J-GLOBAL

• Development of a new marker for Aspergillus transformation using Itraconazole resistance

  渡嘉敷直杏, 水谷治, 水谷治, 外山博英, 外山博英

  日本農芸化学会大会講演要旨集(Web)   2020   2020

   

  J-GLOBAL

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Presentations 【 display / non-display 】

Presentations 【 display / non-display 】

• Awamori, a traditional distilled liquor in Okinawa, and phylogenetic analysis of Aspergillus luchuensis

  Hirohide Toyama

  International Conference on Fermentation Foods 2024 Acetic Acid Bacteria & Monascusx  (Guiyang)  2024.08  -  2024.08 

• Awamori, a traditional distilled liquor in Okinawa, and Moromi-su (Moromi Vinegar)

  Hirohide Toyama

  6th International Symposium on Acetic Acid Bacteria  ( Qingxu city in Shanxi province)  2024.08  -  2024.08 

• Possible peripheral existence of pyrroroquinoline quinone in yeast cells

  2024.03  -  2024.03 

• Awamori, Traditional Distilled Liquor in Okinawa

  Toyama H., Mizutani O., Tsukahara M.

  2018 Korean Traditional Science forum and Exhibition  (EUlLim Hall, Gwachon National Science Museum)  2018.11  -  2018.11 

• 麹菌 ligD 遺伝子がゲノム編集に起因する大規模欠失変異に与える影響

  上元 優, 林 梨咲, 渡嘉敷直杏, 荒添貴之, 山田 修, 外山博英, 水谷 治

  第３回ゲノム編集学会  (広島国際会議場)  2018.06  -  2018.06 

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Grant-in-Aid for Scientific Research 【 display / non-display 】

Grant-in-Aid for Scientific Research 【 display / non-display 】

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2007  -  2007 

  Direct: 1,100,000 (YEN)  Overheads: 330,000 (YEN)  Total: 1,430,000 (YEN)

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2006  -  2007 

  Direct: 2,400,000 (YEN)  Total: 2,400,000 (YEN)