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Research Theme 【 display / non-display 】

• Study on lactic acid bacteria

• Application of Awamori Moromi vinegar

• Analysis of microbes in Awamori Brewing

• Defence Proteins of Tropical Plant

• Relationship Between Structure and Antifungal Activity of Plant Chitinases

Published Papers 【 display / non-display 】

Published Papers 【 display / non-display 】

• Design of Protease-Responsive Antifungal Liposomal Formulation Decorated with a Lipid-Modified Chitin-Binding Domain

  Saputra, H; Safaat, M; Santoso, P; Wakabayashi, R; Goto, M; Taira, T; Kamiya, N

  INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ( International Journal of Molecular Sciences )  25 ( 7 )   2024.04 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Characterization of an antifungal β-1,3-glucanase from <i>Ficus microcarpa</i> latex and comparison of plant and bacterial β-1,3-glucanases for fungal cell wall β-glucan degradation

  Takashima, T; Komori, N; Uechi, K; Taira, T

  PLANTA ( Planta )  258 ( 6 ) 116 - 116   2023.12 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

  　View Summary

  Each β-1,3-glucanase with antifungal activity or yeast lytic activity hydrolyzes different structures of β-1,3-glucans in the fungal cell wall, respectively. Plants express several glycoside hydrolases that target chitin and β-glucan in fungal cell walls and inhibit pathogenic fungal infection. An antifungal β-1,3-glucanase was purified from gazyumaru (Ficus microcarpa) latex, designated as GlxGluA, and the corresponding gene was cloned and expressed in Escherichia coli. The sequence shows that GlxGluA belongs to glycoside hydrolase family 17 (GH17). To investigate how GlxGluA acts to degrade fungal cell wall β-glucan, it was compared with β-1,3-glucanase with different substrate specificities. We obtained recombinant β-1,3-glucanase (designated as CcGluA), which belongs to GH64, from the bacterium Cellulosimicrobium cellulans. GlxGluA inhibited the growth of the filamentous fungus Trichoderma viride but was unable to lyse the yeast Saccharomyces cerevisiae. In contrast, CcGluA lysed yeast cells but had a negligible inhibitory effect on the growth of filamentous fungi. GlxGluA degraded the cell wall of T. viride better than CcGluA, whereas CcGluA degraded the cell wall of S. cerevisiae more efficiently than GlxGluA. These results suggest that the target substrates in fungal cell walls differ between GlxGluA (GH17 class I β-1,3-glucanase) and CcGluA (GH64 β-1,3-glucanase).

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• Analysis of the 4-vinylguaiacol production process in Awamori brewing

  JOURNAL OF THE BREWING SOCIETY OF JAPAN   118 ( 11 ) 757 - 766   2023.11 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

• Liposomal Amphotericin B Formulation Displaying Lipid-Modified Chitin-Binding Domains with Enhanced Antifungal Activity

  Taniguchi, H; Ishimime, Y; Minamihata, K; Santoso, P; Komada, T; Saputra, H; Uchida, K; Goto, M; Taira, T; Kamiya, N

  MOLECULAR PHARMACEUTICS ( Molecular Pharmaceutics )  19 ( 11 ) 3906 - 3914   2022.09 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Preparation of amphotericin B-loaded hybrid liposomes and the integration of chitin-binding proteins for enhanced antifungal activity

  Santoso, P; Komada, T; Ishimine, Y; Taniguchi, H; Minamihata, K; Goto, M; Taira, T; Kamiya, N

  JOURNAL OF BIOSCIENCE AND BIOENGINEERING ( Journal of Bioscience and Bioengineering )  134 ( 3 ) 259 - 263   2022.09 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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Other Papers 【 display / non-display 】

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• Structure and antifungal activity of chitin-degrading enzymes

  Taira Toki, Takashima Tomoya

  Glycoforum ( 生化学工業株式会社 )  26 ( 2 ) A4   2023.04

   

  DOI CiNii Research

• Phenolic acid decarboxylase of <i>Aspergillus luchuensis</i> plays a crucial role in 4-vinylguaiacol production during <i>awamori</i> brewing

  Seibutsu-kogaku Kaishi ( The Society for Biotechnology, Japan )  100 ( 2 ) 79 - 79   2022.02

   

  DOI CiNii Research

• Production of 4-vinylguaiacol, a Vanillin Precursor, by <i>Aspergillus luchuensis</i> in Awamori Brewing

  MAEDA Mayumi, TAIRA Toki

  JOURNAL OF THE BREWING SOCIETY OF JAPAN ( Brewing Society of Japan )  117 ( 5 ) 327 - 334   2022

   

  DOI CiNii Research

Presentations 【 display / non-display 】

Presentations 【 display / non-display 】

• Development of antifungal system based on chitinases

  Toki Taira, Haruna Tsuhako, Shigekazu Yano, Keiko Uechi

  Workshop on Chitin Biology and Beyond  (大連（中国）)  2019.09  -  2019.09 

• Antifungal activity of Pteris ryukyuensis chitinase-A and its enhancement with LysM domain multimers

  Tomoya Takashima, Ryo Sunagawa, Keiko Uechi, Toki Taira

  Workshop on Chitin Biology and Beyond  (大連（中国）)  2019.09  -  2019.09 

• Antifungal activities of LysM domain multimers and their fusion chitinases

  Tomoya Takashima, Keiko Uechi, Toki Taira

  14th International Chitin and Chitosan Conference  (Osaka)  2018.08  -  2018.08 

• Lysin motifs from Plant Chitinases

  Y. Kitaoku, S. Nishimura, T. Numata, W. Suginta, T. Taira, T. Fukamizo, T. Ohnuma

  14th International Chitin and Chitosan Conference  (Osaka)  2018.08  -  2018.08 

• Antifungal activity of chitinases

  Toki Taira

  14th International Chitin and Chitosan Conference  (Osaka)  2018.08  -  2018.08 

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Grant-in-Aid for Scientific Research 【 display / non-display 】

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• Grant-in-Aid for Scientific Research(C)

  Project Year: 2020.04  -  2023.03 

  Direct: 3,200,000 (YEN)  Overheads: 4,160,000 (YEN)  Total: 960,000 (YEN)

• Exploring the novel properties of proteins by controlling the partial hydrophobicity

  Grant-in-Aid for Scientific Research(A)

  Project Year: 2019.04  -  2023.03 

  Direct: 34,900,000 (YEN)  Overheads: 45,370,000 (YEN)  Total: 10,470,000 (YEN)

• Grant-in-Aid for Young Scientists(B)

  Project Year: 2009  -  2010 

  Direct: 2,500,000 (YEN)  Overheads: 750,000 (YEN)  Total: 3,250,000 (YEN)

Preferred joint research theme 【 display / non-display 】

Preferred joint research theme 【 display / non-display 】

• Study on lactic acid bacteria

• Application of Awamori Moromi vinegar

• Analysis of microbes in Awamori Brewing

• Defence Proteins of Tropical Plant

• Relationship Between Structure and Antifungal Activity of Plant Chitinases