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Research Theme 【 display / non-display 】

• Study on lactic acid bacteria

• Application of Awamori Moromi vinegar

• Analysis of microbes in Awamori Brewing

• Defence Proteins of Tropical Plant

• Relationship Between Structure and Antifungal Activity of Plant Chitinases

Published Papers 【 display / non-display 】

Published Papers 【 display / non-display 】

• Characterization of an antifungal β-1,3-glucanase from <i>Ficus microcarpa</i> latex and comparison of plant and bacterial β-1,3-glucanases for fungal cell wall β-glucan degradation

  Takashima, T; Komori, N; Uechi, K; Taira, T

  PLANTA ( Planta )  258 ( 6 ) 116   2023.12 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Analysis of the 4-vinylguaiacol production process in Awamori brewing

  JOURNAL OF THE BREWING SOCIETY OF JAPAN   118 ( 11 ) 757 - 766   2023.11 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

• Liposomal Amphotericin B Formulation Displaying Lipid-Modified Chitin-Binding Domains with Enhanced Antifungal Activity

  Taniguchi, H; Ishimime, Y; Minamihata, K; Santoso, P; Komada, T; Saputra, H; Uchida, K; Goto, M; Taira, T; Kamiya, N

  MOLECULAR PHARMACEUTICS ( Molecular Pharmaceutics )  19 ( 11 ) 3906 - 3914   2022.09 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Preparation of amphotericin B-loaded hybrid liposomes and the integration of chitin-binding proteins for enhanced antifungal activity

  Santoso, P; Komada, T; Ishimine, Y; Taniguchi, H; Minamihata, K; Goto, M; Taira, T; Kamiya, N

  JOURNAL OF BIOSCIENCE AND BIOENGINEERING ( Journal of Bioscience and Bioengineering )  134 ( 3 ) 259 - 263   2022.09 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Structure, mechanism, and phylogeny of LysM-chitinase conjugates specifically found in fern plants

  Kitaoku, Y; Taira, T; Numata, T; Ohnuma, T; Fukamizo, T

  PLANT SCIENCE ( Plant Science )  321   111310 - 111310   2022.08 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

  　View Summary

  A unique GH18 chitinase containing two N-terminal lysin motifs (PrLysM1 and PrLysM2) was first found in fern, Pteris ryukyuensis (Onaga and Taira, Glycobiology, 18, 414-423, 2008). This type of LysM-chitinase conjugates is not usually found in plants but in fungi. Here, we produced a similar GH18 chitinase with one N-terminal LysM module (EaLysM) from the fern, Equisetum arvense (EaChiA, Inamine et al., Biosci. Biotechnol. Biochem., 79, 1296-1304, 2015), using an Escherichia coli expression system and characterized for its structure and mechanism of action. The crystal structure of EaLysM exhibited an almost identical fold (βααβ) to that of PrLysM2. From isothermal titration calorimetry and nuclear magnetic resonance, the binding mode and affinities of EaLysM for chitooligosaccharides (GlcNAc)n (3, 4, 5, and 6) were found to be comparable to those of PrLysM2. The LysM module in EaChiA is likely to bind (GlcNAc)n almost independently through CH-π stacking of a Tyr residue with the pyranose ring. The (GlcNAc)n-binding mode of LysMs in the LysM-chitinase conjugates from fern plants appears to differ from that of plant LysMs acting in chitin- or Nod-signal perception, in which multiple LysMs cooperatively act on (GlcNAc)n. Phylogenetic analysis suggested that LysM-GH18 conjugates of fern plants formed a monophyletic group and had been separated earlier than forming the clade of fungal chitinases with LysMs.

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Other Papers 【 display / non-display 】

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• Structure and antifungal activity of chitin-degrading enzymes

  Taira Toki, Takashima Tomoya

  Glycoforum ( 生化学工業株式会社 )  26 ( 2 ) A4   2023.04

   

  DOI CiNii Research

• Phenolic acid decarboxylase of <i>Aspergillus luchuensis</i> plays a crucial role in 4-vinylguaiacol production during <i>awamori</i> brewing

  Seibutsu-kogaku Kaishi ( The Society for Biotechnology, Japan )  100 ( 2 ) 79 - 79   2022.02

   

  DOI CiNii Research

Presentations 【 display / non-display 】

Presentations 【 display / non-display 】

• Development of antifungal system based on chitinases

  Toki Taira, Haruna Tsuhako, Shigekazu Yano, Keiko Uechi

  Workshop on Chitin Biology and Beyond  (大連（中国）)  2019.09  -  2019.09 

• Antifungal activity of Pteris ryukyuensis chitinase-A and its enhancement with LysM domain multimers

  Tomoya Takashima, Ryo Sunagawa, Keiko Uechi, Toki Taira

  Workshop on Chitin Biology and Beyond  (大連（中国）)  2019.09  -  2019.09 

• Antifungal activities of LysM domain multimers and their fusion chitinases

  Tomoya Takashima, Keiko Uechi, Toki Taira

  14th International Chitin and Chitosan Conference  (Osaka)  2018.08  -  2018.08 

• Lysin motifs from Plant Chitinases

  Y. Kitaoku, S. Nishimura, T. Numata, W. Suginta, T. Taira, T. Fukamizo, T. Ohnuma

  14th International Chitin and Chitosan Conference  (Osaka)  2018.08  -  2018.08 

• Antifungal activity of chitinases

  Toki Taira

  14th International Chitin and Chitosan Conference  (Osaka)  2018.08  -  2018.08 

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Grant-in-Aid for Scientific Research 【 display / non-display 】

Grant-in-Aid for Scientific Research 【 display / non-display 】

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2020.04  -  2023.03 

  Direct: 3,200,000 (YEN)  Overheads: 4,160,000 (YEN)  Total: 960,000 (YEN)

• Exploring the novel properties of proteins by controlling the partial hydrophobicity

  Grant-in-Aid for Scientific Research(A)

  Project Year: 2019.04  -  2023.03 

  Direct: 34,900,000 (YEN)  Overheads: 45,370,000 (YEN)  Total: 10,470,000 (YEN)

• Grant-in-Aid for Young Scientists(B)

  Project Year: 2009  -  2010 

  Direct: 2,500,000 (YEN)  Overheads: 750,000 (YEN)  Total: 3,250,000 (YEN)

Preferred joint research theme 【 display / non-display 】

Preferred joint research theme 【 display / non-display 】

• Study on lactic acid bacteria

• Application of Awamori Moromi vinegar

• Analysis of microbes in Awamori Brewing

• Defence Proteins of Tropical Plant

• Relationship Between Structure and Antifungal Activity of Plant Chitinases