Current Affiliation Organization 【 display / non-display 】

Current Affiliation Organization 【 display / non-display 】

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External Career 【 display / non-display 】

Research Interests 【 display / non-display 】

Research Interests 【 display / non-display 】

Published Papers 【 display / non-display 】

Published Papers 【 display / non-display 】

• Shiga toxin type 2 B subunit protects mice against toxin challenge when leashed and bundled by a stable pentameric coiled-coil molecule.

  Tamaki Y, Harakuni T, Arakawa T

  Vaccine ( Vaccine )  42 ( 7 ) 1757 - 1767   2024.03 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Highly efficient protein expression of Plasmodium vivax surface antigen, Pvs25, by silkworm and its biochemical analysis.

  Miyata T, Minamihata K, Kurihara K, Kamizuru Y, Gotanda M, Obayashi M, Kitagawa T, Sato K, Kimura M, Oyama K, Ikeda Y, Tamaki Y, Lee JM, Sakao K, Hamanaka D, Kusakabe T, Tachibana M, Ibrahim HR

  Protein expression and purification ( Protein Expression and Purification )  195-196   106096   2022.08 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Discovery of Unusual Highly Branched Galactomannan from Seeds of Desmanthus illinoensis

  Masakuni Tako, Yukihiro Tamaki, Takeshi Teruya

  Journal of Biomaterials and Nanobiotechnology   9 ( 2 ) 101 - 116   2018.04 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

• Fiber knob domain lacking the shaft sequence but fused to a coiled coil is a candidate subunit vaccine against egg-drop syndrome.

  Harakuni T, Andoh K, Sakamoto RI, Tamaki Y, Miyata T, Uefuji H, Yamazaki KI, Arakawa T

  Vaccine   34 ( 27 ) 3184 - 3190   2016.06 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Structure-Function Relationship of a Gellan Family of Polysaccharide, S-198 Gum, Produced by Alcaligenes ATCC31853

  Masakuni Tako, Seiko kitajima, Takuya Yogi, Keiko Uechi, Masayuki Onaga, Yukihiro Tamaki, Shuntoku Uechi

  Advances in Biological Chemistry   6 ( 3 ) 55 - 69   2016.05 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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Other Papers 【 display / non-display 】

Other Papers 【 display / non-display 】

• 三日熱マラリア伝搬阻止ワクチン候補抗原(Pvs25)の反復整列化とそのワクチン効果

  原國哲也, 山田清太郎, 宮田健, 山口類, 玉城志博, 坪井敬文, SATTABONGKOT Jetsumon, 橘真由美, 鳥居本美, 新川武

  日本寄生虫学会大会プログラム・抄録集   83rd   70   2014.02

   

  J-GLOBAL

• マラリアワクチン抗原搭載三部構成免疫賦活システム(TIPS)のマラリア伝搬阻止ワクチン機能解析

  玉城志博, 宮田健, 原國哲也, 坪井敬文, JETSUMON Sattabongkot, 橘真由美, 鳥居本美, 新川武

  日本熱帯医学会大会プログラム抄録集   54th   142   2013.09

   

  J-GLOBAL

Grant-in-Aid for Scientific Research 【 display / non-display 】

Grant-in-Aid for Scientific Research 【 display / non-display 】

• Grant-in-Aid for Scientific Research(B)

  Project Year: 2021.04  -  2025.03 

  Direct: 13,200,000 (YEN)  Overheads: 17,160,000 (YEN)  Total: 3,960,000 (YEN)

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2021.04  -  2024.03 

  Direct: 3,200,000 (YEN)  Overheads: 4,160,000 (YEN)  Total: 960,000 (YEN)

• Recombinant toxoid vaccine development against Shiga toxin type 2

  Grant-in-Aid for Scientific Research(C)

  Project Year: 2018.04  -  2021.03 

  Direct: 3,400,000 (YEN)  Overheads: 4,420,000 (YEN)  Total: 1,020,000 (YEN)

• Development of recombinant toxoid vaccines targeting B-subunit against Shiga toxins

  Grant-in-Aid for Young Scientists(B)

  Project Year: 2017.04  -  2019.03 

  Investigator(s): TAMAKI Yukihiro 

  Direct: 3,200,000 (YEN)  Overheads: 4,160,000 (YEN)  Total: 960,000 (YEN)

  　View Summary

  In this study, we aimed to develop a human toxoid vaccine against Shiga toxins (Stx, ie, Stx1 and Stx2), which are AB5 toxins. Fusion proteins linked with B-subunits of Stx1 and Stx2 were constructed to neutralize both Stx1 and Stx2 toxins with one proteinaceous antigen. The order of linking in the fusion proteins (Stx1B-Stx2B and Stx2B-Stx1B) affected the vaccine function, which was related to the pentameric stability of Stx1B and Stx2B.

• Recombinant toxoid vaccine development against Enterotoxigenic Escherichia coli (ETEC)

  Grant-in-Aid for Scientific Research(C)

  Project Year: 2015.04  -  2018.03 

  Investigator(s): ARAKAWA Takeshi 

  Direct: 3,700,000 (YEN)  Overheads: 4,810,000 (YEN)  Total: 1,110,000 (YEN)

  　View Summary

  In this study, we developed a new protein expression and refolding method to produce B subunit pentamer molecules derived from enterotoxins such as cholera toxin (CT), heat-labile enterotoxin (LT) and Shiga toxin (Stx). Those B subunits are to be included in recombinant vaccines to combat against enterotoxin-producing pahthogenic Escherichia coli. Our techniques are considered to be uniuqe in that pentameric B subunit proteins are highly uniform in a molecular structure, although they are often produced as high-molecular mass protein aggregates when refolded from inclustion bodies of E. coli. These findings would be valuable to design molecular vaccines against enteropathogenic E. coli producing LT (enterotoxigenic E. coli: ETEC) or Stx (enterohemorrhagic E. coli: EHEC).