Published Papers - Suzuki Mikio

Human papillomavirus infection and EGFR exon 20 insertions in sinonasal inverted papilloma and squamous cell carcinoma

Hitoshi Hirakawa, Taro Ikegami, Norimoto Kise, Hidetoshi Kinjyo, Shunsuke Kondo, Shinya Agena, Narumi Hasegawa, Junko Kawakami, Hiroyuki, Maeda, Mikio Suzuki

Journal of Personalized Medicine   13 ( 4 ) 657   2023.04 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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[Review] Spatio-temporal gene expression of human papillomavirus in laryngeal papilloma

Taro Ikegami, Asanori Kiyuna, Norimoto Kise, Mikio Suzuki

THE LARYNX JAPAN   33 ( 2 ) 69 - 75   2021.12 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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HPV関連頭頸部癌におけるmTOR経路の検討

近藤 俊輔, 安慶名 信也, 平川 仁, 真栄田 裕行, 鈴木 幹男

日本癌治療学会学術集会抄録集 ( (一社)日本癌治療学会 )  59回   O40 - 1   2021.10 [ Peer Review Accepted ]

Type of publication: Research paper (other science council materials etc.)

HPV非関連進行咽頭癌におけるHIF1-αの発現と予後

安慶名 信也, 近藤 俊輔, 平川 仁, 真栄田 裕行, 鈴木 幹男

日本癌治療学会学術集会抄録集 ( (一社)日本癌治療学会 )  59回   O40 - 3   2021.10 [ Peer Review Accepted ]

Type of publication: Research paper (other science council materials etc.)

A nationwide epidemiologic, clinical, genetic study of Usher syndrome in Japan

Hidekane Yoshimura, Shin ya Nishio, Yuichi Isaka, Toru Kurokawa, Shin ichi Usami, Katada Akihiro, Noriko Ogasawara, Tomoko Shintani, Yumiko Kobayashi, Hiroaki Sato, Seiji Kakehata, Daisuke Kikuchi, Tetsuo Ikezono, Kotaro Ishikawa, Kyoko Shirai, Masahiro Takahashi, Satoshi Iwasaki, Yasuhiro Arai, Hajime Sano, Mayuri Okami, Hiroshi Nakanishi, Tomoko Esaki, Michihiko Sone, Jun Nakayama, Takayuki Okano, Yumi Ota, Hiroshi Nishimura, Yasushi Naito, Yuko Kataoka, Akiko Sugaya, Yukihide Maeda, Shin Masuda, Kazuma Sugawara, Hiroshi Yamashita, Naoto Hato, Takashi Nakagawa, Akira Ganaha, Tetsuya Tono, Ikuyo Miyanohara, Mikio Suzuki

Acta Oto-Laryngologica   141 ( 9 ) 841 - 846   2021 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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Background: Usher syndrome (USH) typically leads to deaf-blindness, requiring the provision of extensive education and rehabilitation services. Therefore, investigating the prevalence is crucial to requests for proper government support for USH patients. Objective: The aim was to perform a nationwide epidemiologic survey of USH in Japan to estimate the prevalence of USH and reveal the relative frequency and characteristics of the three USH subtypes. Methods: To estimate the number of USH patients visiting hospitals over a 1-year period, 1,628 hospitals were randomly selected from all Departments of Otorhinolaryngology and Ophthalmology in Japan. Subsequently, we collected data regarding the clinical characteristics of each patient treated and the results of genetic testing, if performed. Results: We found that the prevalence of USH was at least 0.4 per 100,000 population. The frequency of clinical subtypes and causal genes for USH were consistent with previous reports. Also, we demonstrated the feasibility of genetic counseling for USH patients based on the results of genetic testing. Conclusion: USH is a rare disease, but requires social support due to the severity of symptoms. To minimize these issues, understanding the clinical characteristics and performing comprehensive genetic testing could allow early and accurate diagnosis as well as medical intervention.

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Brain Activity in Patients With Unilateral Vocal Fold Paralysis Detected by Functional Magnetic Resonance Imaging.

Asanori Kiyuna, Norimoto Kise, Munehisa Hiratsuka, Hiroyuki Maeda, Hitoshi Hirakawa, Akira Ganaha, Mikio Suzuki

Journal of voice : official journal of the Voice Foundation     2020.08 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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OBJECTIVES: Understanding brain activity in response to unilateral vocal fold paralysis is essential to determine the neural compensatory mechanism underlying adaptation to voice disorders and to develop novel and improved rehabilitation programs for these disorders. We aimed to clarify brain activity during phonation (prolonged vowel, |i:|) in patients with chronic left vocal fold paralysis (LVFP) and compare with that in normal controls. STUDY DESIGN: Case-control study. METHODS: This functional magnetic resonance imaging (fMRI) study of an event-related task comprised 12 individuals with LVFP of more than 6 months duration and 12 healthy controls. The experimental task alternated phonation (prolonged vowel, |i:|) and no phonation (rest) conditions. The functional images obtained were single-shot gradient-echo echo-planar imaging. The volumes were acquired parallel to the anterior-posterior commissure plane and were sensitive to BOLD contrast. Data sets were processed and statistically analyzed using Statistical Parametric Mapping 8 software. Within-group analyses were conducted by applying the one-sample t test (P < 0.001, uncorrected). A random-effects analysis was used for group comparison. RESULTS: The LVFP group showed significantly higher brain activity in the right premotor areas, left parietal lobule, right primary somatosensory areas, and bilateral supplementary motor area and lower brain activity in the auditory-related areas of the superior temporal gyrus. There were no significant correlations of the percent signal change on fMRI with disease duration, maximum phonation time, or age. CONCLUSION: Patients with chronic unilateral vocal fold paralysis have stronger activity during voluntary phonation in various central networks. More detailed information on the central nervous system regions related to voluntary phonation from early to chronic phase is needed to understand the compensatory mechanisms in vocal fold paralysis and to establish an effective rehabilitation program. This is the first report to investigate brain activity in chronic unilateral vocal fold paralysis.

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Measurement of stapes footplate thickness in otosclerosis by ultra-high-resolution computed tomography.

Yukinori Akazawa, Akira Ganaha, Teruyuki Higa, Shunsuke Kondo, Yoshiki Oyakawa, Hitoshi Hirakawa, Mikio Suzuki, Tsuneo Yamashiro

Acta oto-laryngologica   140 ( 11 ) 1 - 5   2020.07 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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BACKGROUND: Ultra-high-resolution computed tomography (U-HRCT) utilizes a 1024 × 1024 matrix with 0.25-mm section thickness, offering better spatial resolution than conventional multi-detector row CT to detect anatomic data for otologic surgery. AIMS: We examined stapes footplate thickness using U-HRCT in relation to stapedotomy to predict the difficulty of the surgical procedure. MATERIALS AND METHODS: Subjects were 12 otosclerosis patients and 25 controls who underwent diagnostic U-HRCT. A profile curve (Hounsfield units) was used to measure stapes footplate thickness along a perpendicular line across the stapes footplate in a plane parallel to the lateral semicircular canal. RESULTS: Footplate thickness was smaller at the midpoint than just before the anterior crus and just after the posterior crus. Interobserver variability was lowest at the midpoint, where foot plate thickness was significantly greater in the affected ear in otosclerosis patients compared with controls (0.60 ± 0.09 mm vs 0.46 ± 0.04 mm; p < .001). Otosclerosis patients were detected using U-HRCT with a high area under the curve. Difficulty in the stapes opening procedure correlated with stapes footplate thickness. CONCLUSIONS: Footplate thickness on U-HRCT correlated with temporal bone anatomy and corresponded to surgical difficulty. Significance: U-HRCT-derived anatomic data is useful for evaluating the stapes.

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Induction chemotherapy in hypopharyngeal cancers: influences of DNA repair gene polymorphisms

Hirakawa H, Ikegami T, Azechi S, Agena S, Uezato J, Kinjyo H, Yamashita Y, Kiyuna A, Tanaka K, Kondo S, Maeda H, Suzuki M, Ganaha A

Anticancer Research   40 ( in press )   2020.05 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

下咽頭癌におけるDNA修復酵素遺伝子多型と発癌についての検討

疇地里衣, 平川仁, 池上太郎, 長谷川成海, 山下懐, 安慶名信也, 上原貴行, 田中克典, 上里迅, 真栄田裕行, 鈴木幹男

耳鼻と臨床 ( 耳鼻と臨床会 )  66 ( 2 ) 35 - 40   2020.03 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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頭頸部癌の発症とDNA修復酵素遺伝子多型との相関に関し、いまだ結論は出ていない。われわれは沖縄地域において罹患率の高い下咽頭癌の発症が、DNA修復酵素遺伝子多型と関連するかを検討した。琉球大学医学部附属病院耳鼻咽喉科を受診した下咽頭癌患者117例と非癌疾患の患者125例に対し、DNA修復酵素であるERCC1、XPD312、XPD751、XRCC1の遺伝子多型の頻度を比較した。結果は下咽頭癌患者と非癌疾患患者との間で各遺伝子多型発現頻度において有意な差はみられなかった。さらにTNM進行度と遺伝子多型の頻度を比較したところXRCC1多型を有する群において遠隔転移は有意に低かった。XPD312、XPD751に関して欧米諸国と比較したところ変異アレル頻度が低かった。以上より、日本人においてこれらの遺伝子多型は発癌には関連しないが遠隔転移との相関が示唆された。またXPD遺伝子多型には民族間の差があり、特に沖縄地域では変異アレルの頻度が低い可能性があると考えられた。(著者抄録)

ERCC1 C8092A polymorphism predicts fair survival outcome in Japanese patients with pharyngo-laryngeal squamous cell carcinoma.

Hirakawa H, Ikegami T, Azechi S, Agena S, Uezato J, Kinjyo H, Yamashita Y, Tanaka K, Kondo S, Maeda H, Suzuki M, Gahana A

European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery   277 ( 2 ) 601 - 610   2019.11 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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PURPOSE: To evaluate the prognostic significance of DNA excision repair gene polymorphisms, excision repair cross-complementation group 1 (ERCC1) and X-ray repair complementing defective repair in Chinese hamster cells 1 (XRCC1) polymorphisms were investigated in Japanese patients with head and neck squamous cell carcinoma (HNSCC). METHODS: A total of 225 consecutive patients with HNSCC who underwent surgery or chemoradiotherapy/radiotherapy (CRT/RT) with curative intent as primary treatment from 2006 to 2017 were recruited. ERCC1 C8092A and XRCC1 Arg399Gln polymorphisms in DNA extracted from individual blood samples were determined by the polymerase chain reaction-restriction fragment length polymorphism method. Cumulative survival was estimated by Kaplan-Meier analysis with a log-rank test and Cox proportional hazards model stratified by treatment arm, adjusting for clinical prognostic factors. RESULTS: Multivariate analysis showed that carriers with the ERCC1 8092 (C/A+A/A) genotype (hazard ratio, 3.56; 95% confidence interval, 1.22-7.39; p = 0.02) had significantly worse survival than those with ERCC1 8092 C/C who received CRT/RT. Conversely, the XRCC1 Arg399Gln polymorphism did not influence survival in patients who received CRT/RT as well as surgery. CONCLUSION: The ERCC1 C8092A polymorphism might be an independent predictor of response to CRT and survival outcome in patients with HNSCC. This is the first report to investigate the role of DNA excision repair gene polymorphisms in patients with head and neck cancer in a Japanese population.

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頭頸部のヒト乳頭腫ウイルス感染

鈴木 幹男

耳鼻咽喉科臨床 ( 耳鼻咽喉科臨床学会 )  112 ( 10 ) 633 - 639   2019.10 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

Clinical characteristics with long-term follow-up of four Okinawan families with moderate hearing loss caused by an OTOG variant.

Ganaha A, Kaname T, Yanagi K, Tono T, Higa T, Suzuki M

Human genome variation   6   37 - 37   2019.08 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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We describe the clinical features of four Japanese families with moderate sensorineural hearing loss due to the OTOG gene variant. We analyzed 98 hearing loss-related genes in patients with hearing loss originally from the Okinawa Islands using next-generation sequencing. We identified a homozygous variant of the gene encoding otogelin NM_001277269(OTOG): c.330C>G, p.Tyr110* in four families. All patients had moderate hearing loss with a slightly downsloping audiogram, including low frequency hearing loss without equilibrium dysfunction. Progressive hearing loss was not observed over the long-term in any patient. Among the three patients who underwent newborn hearing screening, two patients passed the test. OTOG-associated hearing loss was considered to progress early after birth, leading to moderate hearing loss and the later stable phase of hearing loss. Therefore, there are patients whose hearing loss cannot be detected by NHS, making genetic diagnosis of OTOG variants highly useful for complementing NHS in the clinical setting. Based on the allele frequency results, hearing loss caused by the p.Tyr110* variant in OTOG might be more common than we identified. The p.Tyr110* variant was reported in South Korea, suggesting that this variant is a common cause of moderate hearing loss in Japanese and Korean populations.

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開胸手術直後に歯科用補綴物により頸部食道穿孔をきたした1例

真栄田 裕行, 上里 迅, 島袋 拓也, 仲吉 博紀, 嘉陽 祐紀, 照喜名 玲奈, 金城 秀俊, 安慶名 信也, 又吉 宣, 鈴木 幹男

日本気管食道科学会会報 ( (NPO)日本気管食道科学会 )  70 ( 4 ) 278 - 283   2019.08 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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食道穿孔は日常診療において時に経験する病態であるが,多くの場合生じた穿孔は頸部や縦隔内と交通するため,頸部感染症や縦隔炎を誘発し,時に重篤となることがある.また開胸を伴う胸部外科手術と頸部手術の併施は,術直後の縦隔炎発生の可能性が高まるため,一般には敬遠されている.今回われわれは胸骨正中切開を伴う大動脈弁置換術直後に,歯科用補綴物による頸部食道穿孔をきたした1例を経験した.症例は66歳の女性で主訴は頸部異物である.開胸手術直後に頸部に迷入した異物が確認され,直ちに頸部外切開による異物摘出術および瘻孔閉鎖術が施行された.異物は歯科用補綴物(クラウン)であり,金属製の突起を歯根に埋め込むタイプのものであった.本症例は開胸手術直後であったにもかかわらず頸部外切開手術を全身麻酔下に施行できたこと,異物を速やかに発見・除去できたこと,頸部創と胸部創が連続しないようにしたこと,気管切開を施行せずに術後も気管内挿管のまま気道管理をしたことが重篤な合併症を誘発しなかった理由としてあげられた.(著者抄録)

Hypopharyngeal cancer risk in Japanese: Genetic polymorphisms related to the metabolism of alcohol- and tobacco-associated carcinogens.

Yamashita Y, Ikegami T, Suzuki M, Hirakawa H, Maeda H, Yamada S, Deng Z, Kondo S, Kinjyo H, Kiyuna A, Agena S, Uehara T, Ganaha A

Journal of cancer research and therapeutics ( Medknow )  15 ( 3 ) 556 - 563   2019.07 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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Neck dissections based on sentinel lymph node navigation versus elective neck dissections in early oral cancers: A randomized, multicenter, non-inferiority trial.

Hasegawa Yasuhisa, Tsukahara Kiyoaki, Yoshimoto Seiichi, Miura Kouki, Yokoyama Junkichi, Hirano Shigeru, Uemura Hirokazu, Sugasawa Masashi, Yoshizaki Tomokazu, Homma Akihiro, Chikamatsu Kazuaki, Suzuki Mikio, Shiotani Akihiro, Matsuzuka Takashi, Kohno Naoyuki, Miyazaki Masakazu, Oze Isao, Matsuo Keitaro, Kosuda Shigeru, Yatabe Yasushi

JOURNAL OF CLINICAL ONCOLOGY   37 ( 15 )   2019.05 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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側頸嚢胞におけるハイリスク型・ヒトパピローマウイルス-16の感染

池上 太郎, 上原 貴行, 平川 仁, 真栄田 裕行, 我那覇 章, 鈴木 幹男

耳鼻咽喉科ニューロサイエンス ( 耳鼻咽喉科ニューロサイエンス研究会 )  33   75 - 79   2019.05 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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側頸嚢胞のヒトパピローマウイルス(HPV)感染の有無、ウイルス量、ホストゲノムへのHPV遺伝子の組み込みについて調べた。対象は、2002〜2015年に当科を受診した側頸嚢胞の患者6名(男性3名、女性3名、年齢2〜29歳)とした。HPVの陽性コントロールとしてHPV関連中咽頭癌のリンパ節転移の患者を1名含めた。今回、HPV-16陽性、側頸嚢胞4例では7.10×10^2〜1.65×10^6copies/50ng(median 9.21×10^3)であった。一方、当科の以前の研究では、HPV-16関連扁桃癌においてHPV DNA量は1.54×10^2〜1.34×10^7copies/50ng DNA(median 4.13×10^5)であった。これらの結果から、側頸嚢胞におけるHPV DNA量はHPV関連扁桃癌よりは少なかった。また今回、側頸嚢胞においてHPVの感染の有無に関わらず、p16INK4が嚢胞壁の5%程度の細胞で発現していた。このことから、側頸嚢胞では、少ないp16INK4aの発現はHPV感染のサロゲートマーカーにはならないことが判明した。

頸部腫瘤の診察におけるピットフォール 良性・炎症

鈴木 幹男

日本耳鼻咽喉科学会会報 ( (一社)日本耳鼻咽喉科学会 )  122 ( 4 ) 518 - 518   2019.04

Type of publication: Research paper (scientific journal)

High-risk type human papillomavirus infection and p16 expression in laryngeal cancer.

Kiyuna A, Ikegami T, Uehara T, Hirakawa H, Agena S, Uezato J, Kondo S, Yamashita Y, Deng Z, Maeda H, Suzuki M, Ganaha A

Infectious agents and cancer   14   8 - 8   2019.03 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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BACKGROUND: Oropharyngeal cancers associated with high-risk type human papillomavirus (HR-HPV) infection have better prognosis than virus negative cancers. Similarly, the HPV status in laryngeal cancer (LC) may be associated with better outcome. METHODS: Samples from 88 patients with LC were investigated using the polymerase chain reaction (PCR) and p16 immunohistochemistry for HR-HPV analysis. The cut-off point for p16 overexpression was diffuse (≥75%) tumor expression with at least moderate (+ 2/3) staining intensity. RESULTS: The 5-year cumulative survival (CS) rate was 80.7% in all patients with LC. According to a combination of HR-HPV DNA status and p16 overexpression, subjects with LC were divided into four groups: HR-HPV DNA-positive/p16 overexpression-positive (n = 5, 5.7%; CS = 100%), HR-HPV DNA-positive/p16 overexpression-negative (n = 11, 12.5%; CS =81.8%), HR-HPV DNA-negative/p16 overexpression-positive (n = 0), and HR-HPV DNA-negative/p16 overexpression-negative (n = 72, 81.8%; CS = 79.5%). HR-HPV DNA-positive/p16-positive cases tended to have integrated HPV infection and high viral load, compared with HR-HPV DNA-positive/p16 overexpression-negative cases. CONCLUSIONS: LC patients with HPV infection and high levels of p16 expression might have an improved survival outcome; however, it is necessary to recruit additional LC cases with HPV infection to determine the definitive characteristics of HPV-mediated LC and estimate survival outcome. These results may contribute to the development of a useful method for selecting patients with a potentially fair response to treatment and ensure laryngeal preservation.

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Staging and prognosis of oropharyngeal carcinoma according to the 8th Edition of the American Joint Committee on Cancer Staging Manual in human papillomavirus infection.

Yamashita Y, Ikegami T, Hirakawa H, Uehara T, Deng Z, Agena S, Uezato J, Kondo S, Kiyuna A, Maeda H, Suzuki M, Ganaha A

European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery ( Springer Science and Business Media LLC )  276 ( 3 ) 827 - 836   2019.03 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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HPV関連中咽頭癌（p16陽性）死亡例の検討

金城秀俊, 山下懐, 池上太郎, 嘉陽祐紀, 上里迅, 安慶名信也, 上原貴行, 平川仁, 真栄田裕行, 鈴木幹男

頭頸部癌   45 ( 4 ) 397 - 402   2019 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)