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• Characterization of an α-L-arabinofuranosidase GH51 from the brown-rot fungus <i>Gloeophyllum trabeum</i>

  Tsukida Rikako, Yoshida Makoto, Kaneko Satoshi

  Ｊｏｕｒｎａｌ　ｏｆ　Ａｐｐｌｉｅｄ　Ｇｌｙｃｏｓｃｉｅｎｃｅ ( 日本応用糖質科学会 )  advpub ( 0 )   2022.12 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  <p>Woody biomass is anticipated to be a resource for a decarbonized society, but the difficulty of isolating woody components is a significant challenge. Brown-rot fungi, a type of wood rotting fungi, decompose hemicellulose particularly efficiently. However, there are few reports on the hemicellulases from brown-rot fungi. An α-L-arabinofuranosidase belonging to glycoside hydrolase family 51 (GH51) from the brown-rot fungus <i>Gloeophyllum trabeum</i> (<i>Gt</i>Abf51A) was cloned and characterized in the present study. Analyses of the phylogeny of GH51 enzymes in wood rotting fungi revealed the existence of two groups, intercellular and extracellular enzymes. After deglycosylation, the recombinant <i>Gt</i>Abf51A produced by <i>Pichia pastoris</i> appeared on SDS-PAGE as approximately 71,777 daltons, which is the expected molecular weight based on the amino acid sequence of <i>Gt</i>Abf51A. Maximum enzyme activity occurred between pH 2.2 and 4.0 and at 50°C, while it was stable between pH 2.2 and 10.0 and up to 40°C. Due to the presence of a signal peptide, <i>Gt</i>Abf51A was thought to hydrolyze polysaccharide containing arabinose. However, the hydrolysis rate of arabinosyl linkages in polysaccharides was only 3%–4% for arabinoxylan and 17% for arabinan. <i>Gt</i>Abf51A, in contrast, efficiently hydrolyzed arabinoxylooligosaccharides, particularly <i>O</i>-α-L-arabinofuranosyl-(1→3)-<i>O</i>-β-D-xylopyranosyl-(1→4)-β-Dxylopyranose, which is the principal product of GH10 β-xylanase. These data suggest that <i>Gt</i>Abf51A cooperates with other xylan-degrading enzymes, such as β-xylanase, to degrade xylan in nature.</p>

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• Microorganisms capable of producing polysaccharides from D-xylose

  Tsutsui Sosyu, Hatano Tomohiro, Funada Ryo, Kaneko Satoshi

  Ｊｏｕｒｎａｌ　ｏｆ　Ａｐｐｌｉｅｄ　Ｇｌｙｃｏｓｃｉｅｎｃｅ ( 日本応用糖質科学会 )  advpub ( 0 )   2022.10 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  <p>In recent years, the importance of biomass utilization has increased, but it has not been effectively exploited. In particular, it is difficult to use hemicellulose, the second most abundant biopolymer of biomass. Therefore, in order to promote the utilization of hemicellulose, we screened for microorganisms capable of producing polysaccharides from xylose. The following four strains were selected from samples collected from various regions of Okinawa Prefecture: <i>Kosakonia</i> sp. (SO_001), <i>Papiliotrema terrestris</i> (SO_005), <i>Pseudoarthrobacter</i> sp. (SO_006), and <i>Williamsia</i> sp. (SO_009). Observation with a scanning electron microscope (SEM) confirmed that each bacterium produced polysaccharides with different shapes. In addition, the molecular weight and sugar composition of the polysaccharides produced by each bacterium were distinct. The selected microorganisms include closely related species known to promote plant growth and known to suppress postharvest pathogens. Since these microorganisms may be used not only in known fields but also in new fields, the results of this research are expected to greatly expand the uses of hemicellulose.</p>

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• Molecular and biochemical characteristics of inulosucrase InuBK from Alkalihalobacillus krulwichiae JCM 11691.

  Yokoi KJ, Tsutsui S, Arakawa GY, Takaba M, Fujii K, Kaneko S

  Bioscience, biotechnology, and biochemistry ( Bioscience, Biotechnology and Biochemistry )  85 ( 8 ) 1830 - 1838   2021.07 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Substrate Specificities of GH8, GH39, and GH52 β-xylosidases from Bacillus halodurans C-125 Toward Substituted Xylooligosaccharides.

  Teramoto K, Tsutsui S, Sato T, Fujimoto Z, Kaneko S

  Applied biochemistry and biotechnology ( Applied Biochemistry and Biotechnology )  193 ( 4 ) 1042 - 1055   2021.04 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Structure-based substrate specificity analysis of GH11 xylanase from Streptomyces olivaceoviridis E-86.

  Fujimoto Z, Kishine N, Teramoto K, Tsutsui S, Kaneko S

  Applied microbiology and biotechnology ( Applied Microbiology and Biotechnology )  105 ( 5 ) 1943 - 1952   2021.03 [ Peer Review Accepted ]

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Other Papers 【 display / non-display 】

Other Papers 【 display / non-display 】

• Crystal structure of Streptomyces olivaceoviridis

  J. Mol. Biol. 300, 575-585 (2000)     1900.01

   

  DOI

• Enhancement of thermostability and hydrolytic activity of xylanase by random gene shuffling

  Biochem. J. 349, 651-656 (2000)     1900.01

   

  DOI

SDGs 【 display / non-display 】

SDGs 【 display / non-display 】