論文 - 屋　宏典

Molecular characteristics of isoprene synthase and its control effects on isoprene emissions from tropical trees.

Oku H, Mutanda I, Inafuku M

Journal of plant research   136 ( 1 ) 63 - 82   2023年01月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Fractionation and characterization of cell wall polysaccharides from the brown alga Cladosiphon okamuranus.

Awanthi MGG, Umosa M, Yuguchi Y, Oku H, Kitahara K, Ito M, Tanaka A, Konishi T

Carbohydrate research   523   108722   2023年01月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Stress amelioration and anti-inflammatory potential of Shiikuwasha (Citrus depressa Hayata) essential oil, limonene, and γ-terpinene

Yonathan Asikin, Kuniyoshi Shimizu, Hironori Iwasaki, Hirosuke Oku, Koji Wada

Journal of Food and Drug Analysis ( The Journal of Food and Drug Analysis (JFDA), Food and Drug Administration, Taiwan (TFDA) )  30 ( 3 ) 454 - 465   2022年09月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Isolation and characterization of mimosine degrading enzyme from Arthrobacter sp. Ryudai-S1.

Shigeki Oogai, Masakazu Fukuta, Masashi Inafuku, Hirosuke Oku

World journal of microbiology & biotechnology   38 ( 10 ) 172 - 172   2022年07月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

Leucaena leucocephala growing in the tropics and subtropics serves as potential forage for livestock because its foliage is rich in protein, fiber, and minerals. However, its use for livestock feed has been hindered by toxic nonprotein amino acid mimosine. Therefore, it is necessary to develop a method to reduce or eliminate mimosine from foliage. A previous study found that the fermentation of L. leucocephala foliage reduced the mimosine content and prompted the authors to isolate potent mimosine degrading microorganisms and characterize the mimosinase for the complete elimination of mimosine in the L. leucocephala foliage. The soil screening of the L. leucocephala tree surroundings led to the isolation of Arthrobacter sp. Ryudai-S1, which can degrade and assimilate mimosine as a nitrogen and carbon source. Mimosinase in this strain was found to be thermostable and showed strong activity. Docking model's inspection and the interaction energy calculation between mimosine-pyridoxal-5'-phosphate (PLP) complex and the active site of this enzyme identified 11 important amino acid residues that stabilized the binding. Of these amino acid residues, mutation experiment suggested that Tyr-263' and Phe-34 stabilizes the substrate binding and play a critical role in guiding the substrate to proper positions to accomplish high catalytic efficacy and selectivity. These observations suggest that Arthrobacter sp. Ryudai-S1 could be potentially useful for the development of L. leucocephala feed with reduced mimosine content.

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Plant hormone profile and control over isoprene biosynthesis in a tropical tree Ficus septica.

Iqbal MA, Miyamoto K, Yumoto E, Parveen S, Mutanda I, Inafuku M, Oku H

Plant biology (Stuttgart, Germany)   24 ( 3 ) 492 - 501   2022年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Plant hormone signalling and the circadian clock have been implicated in the transcriptional control of isoprene biosynthesis. To gain more insight into the hormonal control of isoprene biosynthesis, the present study measured plant hormone concentrations in jasmonic acid (JA)-treated leaves of our previous model study, examined their relationship with gene expression of isoprene synthase (IspS) and hormone signalling transcription factors. Of the plant hormones, IAA and JA-Ile and their related transcription factors (MYC2 and SAUR21) were significantly correlated with IspS gene expression. Concentrations of cytokinins, isopentenyladenine (iP), trans-zeatin riboside (tZR) and cis-zeatin riboside (cZR), were similarly significantly correlated with IspS expression. However, there was no significant correlation between their related transcription factor (ARR-B) and IspS expression. The circadian clock-related gene PRR7, but not the transcription factor LHY, was highly correlated with IspS expression. These results suggest that the hormonal balance between JA-Ile and IAA plays a central role in transcriptional regulation of IspS through the transcription factors MYC2 and SAUR21, the early auxin responsive genes. The putative cis-acting elements for SAUR on the IspS promoter (TGTCNN and CATATG), in addition to the G-box for MYC2, support the above proposal. These results provide insightful information on the core components of plant hormone-related regulation of IspS under coordination with the circadian clock genes.

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Non-enzymatic formation of isoprene and 2-methyl-3-buten-2-ol (2-MBO) by manganese.

Oku H, Mutanda I, Fukuta M, Inafuku M

Scientific reports   12 ( 1 ) 2465 - 2465   2022年02月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

It has been suggested that isoprene synthesis by isoprene synthase (IspS) proceeds via a substrate-assisted mechanism. The authors observed a non-enzymatic isoprene formation by Mn2+, which represents the basis of IspS enzyme reaction. Because IspS and many other terpene synthases require Mn2+ metal ions as cofactor, this study characterized the formation reaction for the first time. Metal ions including Mn2+ non-enzymatically produced both isoprene and 2-methyl-3-buten-2-ol (2-MBO) from dimethylallyl pyrophosphate (DMADP). Isoprene formation was most enhanced by Fe2+ and, to a lesser extent, by Mn2+ or Cu2+. Ni2+, Co2+, Mg2+, and Ba2+ exhibited a low activity to generate both isoprene and 2-MBO. The proportion of isoprene and 2-MBO varied with the Mn2+ concentration: isoprene predominated over 2-MBO at a higher Mn2+ concentration. Similarly, isoprene formation by Mn2+ increased exponentially as temperature increased with predominance of isoprene over 2-MBO at higher temperature. Both isoprene and 2-MBO formation was enhanced by acidic and neutral pH compared to alkaline conditions. Molecular dynamic simulation of DMADP suggested that the formation reaction is initiated by deprotonation of hydrogen on allyl terminal carbon by phosphate oxygen and generates carbocation and allyl anion intermediates. This is followed by quenching to produce isoprene or by hydroxyl addition to form 2-MBO. Thus, this study provided an insight into reaction mechanism of isoprene and 2-MBO biosynthesis and highlighted some parts of isoprene emission from terrestrial plants, which could be formed by non-enzymatic mechanism.

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Growth condition controls on G-93 parameters of isoprene emission from tropical trees.

Oku H, Iwai S, Uehara M, Iqbal A, Mutanda I, Inafuku M

Journal of plant research ( Journal of Plant Research )  134 ( 6 ) 1225 - 1242   2021年11月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

Despite its major role in global isoprene emission, information on the environmental control of isoprene emission from tropical trees has remained scarce. Thus, in this study, we examined the relationship between parameters of G-93 isoprene emission formula (CT1, CT2, and α), growth temperature and light intensity, photosynthesis (ɸ, Pmax), isoprene synthase (IspS) level, and 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway metabolites using sunlit and shaded leaves of four tropical trees. The results showed that the temperature dependence of isoprene emission from shaded leaves did not differ significantly from sunlit leaves. In contrast, there was a lower saturation irradiance in shaded leaves than in sunlit leaves, the same as temperate plants. The photosynthesis rate of shaded leaves showed lower saturation irradiance, similar to the light dependence of isoprene emission. In most cases, the concentration of MEP pathway metabolites was of lower tendency in shaded leaves versus in sunlit leaves, whereas no significant difference was noted in IspS level between sunlit and shaded leaves. Correlation analysis between these parameters found that CT1 of the G-93 parameter was positively correlated with the concentration of DXP and DMADP, whereas CT2 correlated with the concentration of MEP and the average air temperature for the past 48 h. Similarly, α closely associated with the initial slope (ɸ) of photosynthesis rate, and the basal emission factor is also linked to the photon flux of past days. These results suggest that growth conditions may control the temperature dependence of isoprene emission from tropical trees via the changes in the profiles of MEP pathway metabolites, causing alteration in the parameters of the isoprene emission formula.

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Mechanism of the fungal-like particles in the inhibition of adipogenesis in 3T3-L1 adipocytes.

Jakkawanpitak C, Inafuku M, Oku H, Hutadilok-Towatana N, Bunkrongcheap R, Sermwittayawong N, Aiemchareon P, Sermwittayawong D

Scientific reports ( Scientific Reports )  11 ( 1 ) 18869 - 18869   2021年09月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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The dynamic ability of adipocytes in adipose tissue to store lipid in response to changes in the nutritional input and inflammatory elicitors has a major impact on human health. Previously, we established laminarin-coated beads or LCB as an inflammatory elicitor for adipocytes. However, it was not clear whether LCB inhibits lipid accumulation in adipocytes. Here, we show that LCB acts in the early stage of adipogenesis through both interleukin-1 receptor-associated kinases (IRAK) and spleen tyrosine kinase (SYK) pathways, resulting in the activation of the AMP-activated protein kinase (AMPK) and nuclear factor-κB (NF-κB) complexes, which subsequently cause cell cycle arrest, downregulation of the key transcription factors and enzymes responsible for adipogenesis, inhibition of adipogenesis, and stimulation of an inflammatory response. While LCB could effectively block lipid accumulation during the early stage of adipogenesis, it could stimulate an inflammatory response at any stage of differentiation. Additionally, our results raise a possibility that toll-like receptor 2 (TLR2) and C-type lectin domain family 7 member A (CLEC7A/Dectin-1) might be potential β-glucan receptors on the fat cells. Together, we present the mechanism of LCB, as fungal-like particles, that elicits an inflammatory response and inhibits adipogenesis at the early stage of differentiation.

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The dose-dependent pteryxin-mediated molecular mechanisms in suppressing adipogenesis in vitro

Nugara R.N.

Journal of Functional Foods ( Journal of Functional Foods )  82   2021年07月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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The aim was to perform an in-depth characterization of pteryxin in the anti-obesity related molecular mechanisms using 3T3-L1 preadipocytes. Cells were treated with pteryxin 20 (PTX20) or 50 (PTX50) μg/mL during cell differentiation or maintenance period. Pteryxin dose-dependently suppressed adipogenic gene network. PTX20 downregulated key lipogenic genes suppressing fatty acid synthesis allotting the anti-adipogenesis in the late adipocyte formation stage. PTX50 downregulated adipogenesis related genes, and accelerated lipolytic activity by repressing the adipocyte size marker gene, MEST. In addition to Wnt5a, PTX50 suppressed adipocyte differentiation through TGF-β/Smad3 signaling pathway with the maximum adiposity inhibition in the early stage of adipogenesis and regulated the carbohydrate metabolism. Pteryxin regulated phosphorylation of ERK1/2 and AMPK, and decreased p38 MAPK to repress the mature adipocyte formation. Overall, we have demonstrated the anti-adipogenic characteristics of pteryxin consistent to its dose. PTX50 may serve as a potential candidate to ameliorate adiposity and improve the carbohydrate metabolism.

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Nitric Oxide Regulates Plant Growth, Physiology, Antioxidant Defense, and Ion Homeostasis to Confer Salt Tolerance in the Mangrove Species, <i>Kandelia obovata</i>.

Hasanuzzaman M, Inafuku M, Nahar K, Fujita M, Oku H

Antioxidants (Basel, Switzerland) ( Antioxidants )  10 ( 4 )   2021年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Facultative halophyte Kandelia obovata plants were exposed to mild (1.5% NaCl) and severe (3% NaCl) salt stress with or without sodium nitroprusside (SNP; 100 µM; a NO donor), hemoglobin (Hb, 100 µM; a NO scavenger), or Nω-nitro-L-arginine methyl ester (L-NAME, 100 µM; a NO synthase inhibitor). The plants were significantly affected by severe salt stress. They showed decreases in seedling growth, stomatal conductance, intercellular CO2 concentration, SPAD value, photosynthetic rate, transpiration rate, water use efficiency, and disrupted antioxidant defense systems, overproduction of reactive oxygen species, and visible oxidative damage. Salt stress also induced ion toxicity and disrupted nutrient homeostasis, as indicated by elevated leaf and root Na+ contents, decreased K+ contents, lower K+/Na+ ratios, and decreased Ca contents while increasing osmolyte (proline) levels. Treatment of salt-stressed plants with SNP increased endogenous NO levels, reduced ion toxicity, and improved nutrient homeostasis while further increasing Pro levels to maintain osmotic balance. SNP treatment also improved gas exchange parameters and enhanced antioxidant enzymes’ activities (catalase, ascorbate peroxidase, monodehydroascorbate reductase, and dehydroascorbate reductase). Treatment with Hb and L-NAME reversed these beneficial SNP effects and exacerbated salt damage, confirming that SNP promoted stress recovery and improved plant growth under salt stress.

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Syringin: A phenylpropanoid glycoside compound in Cirsium brevicaule A. GRAY root modulates adipogenesis

Abu Yousuf Hossin, Masashi Inafuku, Kensaku Takara, Ruwani N. Nugara, Hirosuke Oku

Molecules   26 ( 6 )   2021年03月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Cirsium brevicaule A. GRAY is a wild perennial herb, and its roots (CbR) have traditionally been used as both food and medicine on the Japanese islands of Okinawa and Amami. The present study evaluated the antiadipogenic effect of CbR using mouse embryonic fibroblast cell line 3T3-L1 from JCRB cell bank. Dried CbR powder was serially extracted with solvents of various polarities, and these crude extracts were tested for antiadipogenic activity. Treatment with the methanol extract of CbR showed a significant suppression of lipid accumulation in 3T3-L1 cells. Methanol extract of CbR was then fractionated and subjected to further activity analyses. The phenylpropanoid glycosidic molecule syringin was identified as an active compound. Syringin dose dependently suppressed lipid accumulation of 3T3-L1 cells without cytotoxicity, and significantly reduced the expressions of peroxisome proliferator-activated receptor gamma, the master regulator of adipogenesis, and other differentiation markers. It was demonstrated that syringin effectively enhanced the phosphorylation of the AMP-activated protein kinase and acetyl-CoA carboxylase. These results indicate that syringin attenuates adipocyte differentiation, adipogenesis, and promotes lipid metabolism; thus, syringin may potentially serve as a therapeutic candidate for treatment of obesity.

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Anticancer Effects of Polyisoprenoid From Nypa fruticans Leaves by Controlling Expression of p53, EGFR, PI3K, AKT1, and mTOR Genes in Colon Cancer (WiDr) Cells

Meighina Atika Istiqomah, Poppy Anjelisa, Zaitun Hasibuan, Sumaiyah Sumaiyah, Era Yusraini, Hirosuke Oku, Mohammad Basyuni

Natural Product Communications   15 ( 4 ) 1 - 8   2020年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

Molecular cloning of putative chloroplastic cysteine synthase in Leucaena leucocephala.

Harun-Ur-Rashid M, Oogai S, Parveen S, Inafuku M, Iwasaki H, Fukuta M, Amzad Hossain M, Oku H

Journal of plant research ( Journal of Plant Research )  133 ( 1 ) 95 - 108   2020年01月 [ 査読有り ]

掲載種別: 研究論文（その他学術会議資料等）

　概要を見る

Cysteine biosynthesis is directed by the successive commitments of serine acetyltransferase, and O-acetylserine (thiol) lyase (OASTL) compounds, which subsequently frame the decameric cysteine synthase complex. The isoforms of OASTL are found in three compartments of the cell: the cytosol, plastid, and mitochondria. In this investigation, we first isolated putative chloroplastic OASTL (Ch-OASTL) from Leucaena leucocephala, and the Ch-OASTL was then expressed in BL21-competent Escherichia coli. The putative Ch-OASTL cDNA clone had 1,543 base pairs with 391 amino acids in its open reading frame and a molecular weight of 41.54 kDa. The purified protein product exhibited cysteine synthesis ability, but not mimosine synthesis activity. However, they both make the common α-aminoacrylate intermediate in their first half reaction scheme with the conventional substrate O-acetyl serine (OAS). Hence, we considered putative Ch-OASTL a cysteine-specific enzyme. Kinetic studies demonstrated that the optimum pH for cysteine synthesis was 7.0, and the optimum temperature was 40 °C. In the cysteine synthesis assay, the Km and kcat values were 838 ± 26 µM and 72.83 s-1 for OAS, respectively, and 60 ± 2 µM and 2.43 s-1 for Na2S, respectively. We can infer that putative Ch-OASTL regulatory role is considered a sensor for sulfur constraint conditions, and it acts as a forerunner of various metabolic compound molecules.

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Dihydropyranocoumarins Exerted Anti-Obesity Activity In Vivo and its Activity Was Enhanced by Nanoparticulation with Polylactic-Co-Glycolic Acid.

Hossin AY, Inafuku M, Oku H

Nutrients ( Nutrients )  11 ( 12 )   2019年12月 [ 査読有り ]

掲載種別: 研究論文（その他学術会議資料等）

　概要を見る

Dihydropyranocoumarins (DPCs) were isolated from Peucedanumjaponicum Thunb as anti-obesity compounds in 3T3-L1 adipocytes assay; however, it is uncertain whether DPC exerts anti-obesity activity in vivo. Therefore, this study evaluated the oral intake of pure DPCs in mice fed a high-fat diet, and also attempted to enhance its activity by nanoparticulation. Increases in body weight gain and fat accumulation in white adipose tissues were significantly suppressed by the dietary intake of DPCs (1.943 mg/mouse/day). DPCs intake also significantly decreased the mean size of adipocytes and upregulated mRNA levels of thermogenesis-related genes. Nanoparticulation of DPCs with polylactic-co-glycolic acid (PLGA) dramatically increased its activity almost 100-fold over that of a non-nanoparticulated form. Thus, our findings clearly demonstrated the anti-obesity activity of DPCs in vivo and suggested that PLGA nanoparticle encapsulation was useful to enhance the anti-obesity activity of DPCs with the aim to develop natural and safe anti-obesity agents.

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Effect of purified soluble polysaccharides extracted from gray oyster mushroom [pleurotus sajor-caju (fr.) sing.] on 3t3-l1 adipocytes

Sermwittayawong D.

Sains Malaysiana ( Sains Malaysiana )  49 ( 1 ) 103 - 112   2019年10月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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© 2020 Penerbit Universiti Kebangsaan Malaysia. All rights reserved. Functional mushroom polysaccharides have been known to the Asian traditional medicine for many years. The anti-obesity activity of mushroom polysaccharides has been illustrated both in vivo and in vitro. However, the effect of pure polysaccharides on isolated fat cells has not been demonstrated. In this study, we used hot water and ammonium oxalate to extract polysaccharides from gray oyster mushroom [Pleurotus sajor-caju (Fr.) Sing.] and subsequently purified with enzymatic digestion, solvent extraction and ion-exchange chromatography. One of the fractions, 7S1-1, has the highest yield and purity and contains polysaccharides with the molecular weight around 500 kDa. In addition, Fourier transformed infrared spectroscopy (FTIR) and monosaccharide composition analyses showed that this 7S1-1 sample contains mostly β-glucan and some mannan. We find that the 7S1-1 fraction does not affect the total amount of triglycerides in the 3T3-L1 adipocytes but promotes a release of glycerol of the fat cells, suggesting that the fraction does not inhibit adipogenesis but stimulates lipolysis. Laminarin, a small β-glucan, confers similar results to the 7S1-1 sample, but it could not stimulate lipolysis as well as the 7S1-1 sample. Therefore, these results suggest a potential anti-obesity activity of the mushroom polysaccharides.

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Molecular characterization of mimosinase and cystathionine β-lyase in the Mimosoideae subfamily member Mimosa pudica.

Oogai S, Fukuta M, Watanabe K, Inafuku M, Oku H

Journal of plant research ( Journal of Plant Research )  132 ( 5 ) 667 - 680   2019年09月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Plant hormone effects on isoprene emission from tropical tree in Ficus septica.

Parveen S, Iqbal MA, Mutanda I, Rashid MH, Inafuku M, Oku H

Plant, cell & environment ( Plant Cell and Environment )  42 ( 5 ) 1715 - 1728   2019年05月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Molecular regulatory mechanism of isoprene emission under short-term drought stress in the tropical tree Ficus septica.

Parveen S, Rashid MH, Inafuku M, Iwasaki H, Oku H

Tree physiology ( Tree Physiology )  39 ( 3 ) 440 - 453   2019年03月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

Isoprene is emitted by many plants and is thought to function as an antioxidant under stressful conditions. However, the detailed regulatory mechanism of isoprene emission in relation to the antioxidant system remains unclear. Therefore, in this study, we explored the molecular regulatory mechanism of isoprene emission under short-term drought stress in the tropical tree Ficus septica Burm.f. We found that the soil moisture content gradually decreased from 55% on Day 1 (D1) to 23% (wilting point) on D5 after withholding water for 4 days and then returning to the initial level following re-watering on D6. On D5, drought-stressed plants had more than twofold higher isoprene emission and 90.6% lower photosynthesis rates, 99.5% lower stomatal conductance and 82.3% lower transpiration rates than well-watered control plants. It was also estimated that the isoprene concentration inside the leaf greatly increased on D5 due to the increased isoprene emission rate and reduced stomatal conductance. Among the traits related to the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway, which is responsible for isoprene biosynthesis, the isoprene synthase (IspS) protein level was positively correlated with the isoprene emission rate in stressed plants. The transcripts of the antioxidant genes peroxidase 2 (POD2), POD4, copper-zinc superoxide dismutase 2 (Cu-ZnSOD2) and manganese superoxide dismutase 1 (Mn-SOD1) also increased during the drying period, while those of ascorbate peroxidase 1 (APX1) decreased. However, there was only a weak correlation between isoprene emission and antioxidant enzyme gene expression, indicating that the regulation of isoprene biosynthesis is not directly linked to the antioxidant defense network in drought-stressed F. septica. These findings suggest that the post-transcriptional regulation of IspS led to the observed change in isoprene emission rate, which enhanced the quenching of reactive oxygen species (ROS) and, in combination with the increased antioxidant enzyme activity, conferred tolerance to drought stress in this species.

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Inhibition of Niemann-Pick C1-Like 1 by Ezetimibe Reduces Dietary 5β,6β-Epoxycholesterol Absorption in Rats.

Shirouchi B, Furukawa Y, Nakamura Y, Kawauchi A, Imaizumi K, Oku H, Sato M

Cardiovascular drugs and therapy ( Cardiovascular Drugs and Therapy )  33 ( 1 ) 35 - 44   2019年02月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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© 2019, Springer Science+Business Media, LLC, part of Springer Nature. Purpose: Oxycholesterols (OCs) are produced from cholesterol by oxidation of the steroidal backbone and side-chain. OCs are present in blood and evidence suggests their involvement in disease development and progression. However, limited information is available regarding the absorption mechanisms and relative absorption rates of dietary OCs. Although ezetimibe is known to inhibit intestinal cholesterol absorption via Niemann-Pick C1-Like 1 (NPC1L1), whether it also inhibits dietary OC absorption is unclear. Methods: We investigated the effects of ezetimibe on OC absorption in rats fed an OC-rich diet containing 10 different OCs. We collected lymphatic fluid using permanent cannulation of the thoracic duct and quantified OC levels. Results: Ezetimibe treatment significantly reduced the apparent absorption of 5β,6β-epoxycholesterol (5,6β-epoxy) and its levels in the proximal intestinal mucosa in OC-fed rats. Using in silico analyses, the binding energy of NPC1L1 N-terminal domain (NPC1L1-NTD) and 5,6β-epoxy was found to be similar to that of NPC1L1-NTD and cholesterol, suggesting that polar uncharged amino acids located in the steroidal part of 5,6β-epoxy were involved. Conclusion: Our results indicate that ezetimibe-mediated inhibition of dietary OC absorption varies depending on the specific OC, and only the absorption of 5,6β-epoxy is significantly reduced.

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Evaluation of isoprene emission rates of tropical trees by an iterative optimization procedure for G-93 parameters

Higa T.

Atmospheric Environment ( Atmospheric Environment )  192   209 - 217   2018年11月

掲載種別: 研究論文（学術雑誌）

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