論文 - 屋　宏典

Molecular characteristics of isoprene synthase and its control effects on isoprene emissions from tropical trees.

Oku H, Mutanda I, Inafuku M

Journal of plant research   136 ( 1 ) 63 - 82   2023年01月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Fractionation and characterization of cell wall polysaccharides from the brown alga Cladosiphon okamuranus.

Awanthi MGG, Umosa M, Yuguchi Y, Oku H, Kitahara K, Ito M, Tanaka A, Konishi T

Carbohydrate research   523   108722   2023年01月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Stress amelioration and anti-inflammatory potential of Shiikuwasha (Citrus depressa Hayata) essential oil, limonene, and γ-terpinene

Yonathan Asikin, Kuniyoshi Shimizu, Hironori Iwasaki, Hirosuke Oku, Koji Wada

Journal of Food and Drug Analysis ( The Journal of Food and Drug Analysis (JFDA), Food and Drug Administration, Taiwan (TFDA) )  30 ( 3 ) 454 - 465   2022年09月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Isolation and characterization of mimosine degrading enzyme from Arthrobacter sp. Ryudai-S1.

Shigeki Oogai, Masakazu Fukuta, Masashi Inafuku, Hirosuke Oku

World journal of microbiology & biotechnology   38 ( 10 ) 172 - 172   2022年07月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

Leucaena leucocephala growing in the tropics and subtropics serves as potential forage for livestock because its foliage is rich in protein, fiber, and minerals. However, its use for livestock feed has been hindered by toxic nonprotein amino acid mimosine. Therefore, it is necessary to develop a method to reduce or eliminate mimosine from foliage. A previous study found that the fermentation of L. leucocephala foliage reduced the mimosine content and prompted the authors to isolate potent mimosine degrading microorganisms and characterize the mimosinase for the complete elimination of mimosine in the L. leucocephala foliage. The soil screening of the L. leucocephala tree surroundings led to the isolation of Arthrobacter sp. Ryudai-S1, which can degrade and assimilate mimosine as a nitrogen and carbon source. Mimosinase in this strain was found to be thermostable and showed strong activity. Docking model's inspection and the interaction energy calculation between mimosine-pyridoxal-5'-phosphate (PLP) complex and the active site of this enzyme identified 11 important amino acid residues that stabilized the binding. Of these amino acid residues, mutation experiment suggested that Tyr-263' and Phe-34 stabilizes the substrate binding and play a critical role in guiding the substrate to proper positions to accomplish high catalytic efficacy and selectivity. These observations suggest that Arthrobacter sp. Ryudai-S1 could be potentially useful for the development of L. leucocephala feed with reduced mimosine content.

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Plant hormone profile and control over isoprene biosynthesis in a tropical tree Ficus septica.

Iqbal MA, Miyamoto K, Yumoto E, Parveen S, Mutanda I, Inafuku M, Oku H

Plant biology (Stuttgart, Germany)   24 ( 3 ) 492 - 501   2022年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

Plant hormone signalling and the circadian clock have been implicated in the transcriptional control of isoprene biosynthesis. To gain more insight into the hormonal control of isoprene biosynthesis, the present study measured plant hormone concentrations in jasmonic acid (JA)-treated leaves of our previous model study, examined their relationship with gene expression of isoprene synthase (IspS) and hormone signalling transcription factors. Of the plant hormones, IAA and JA-Ile and their related transcription factors (MYC2 and SAUR21) were significantly correlated with IspS gene expression. Concentrations of cytokinins, isopentenyladenine (iP), trans-zeatin riboside (tZR) and cis-zeatin riboside (cZR), were similarly significantly correlated with IspS expression. However, there was no significant correlation between their related transcription factor (ARR-B) and IspS expression. The circadian clock-related gene PRR7, but not the transcription factor LHY, was highly correlated with IspS expression. These results suggest that the hormonal balance between JA-Ile and IAA plays a central role in transcriptional regulation of IspS through the transcription factors MYC2 and SAUR21, the early auxin responsive genes. The putative cis-acting elements for SAUR on the IspS promoter (TGTCNN and CATATG), in addition to the G-box for MYC2, support the above proposal. These results provide insightful information on the core components of plant hormone-related regulation of IspS under coordination with the circadian clock genes.

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Non-enzymatic formation of isoprene and 2-methyl-3-buten-2-ol (2-MBO) by manganese.

Oku H, Mutanda I, Fukuta M, Inafuku M

Scientific reports   12 ( 1 ) 2465 - 2465   2022年02月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

It has been suggested that isoprene synthesis by isoprene synthase (IspS) proceeds via a substrate-assisted mechanism. The authors observed a non-enzymatic isoprene formation by Mn2+, which represents the basis of IspS enzyme reaction. Because IspS and many other terpene synthases require Mn2+ metal ions as cofactor, this study characterized the formation reaction for the first time. Metal ions including Mn2+ non-enzymatically produced both isoprene and 2-methyl-3-buten-2-ol (2-MBO) from dimethylallyl pyrophosphate (DMADP). Isoprene formation was most enhanced by Fe2+ and, to a lesser extent, by Mn2+ or Cu2+. Ni2+, Co2+, Mg2+, and Ba2+ exhibited a low activity to generate both isoprene and 2-MBO. The proportion of isoprene and 2-MBO varied with the Mn2+ concentration: isoprene predominated over 2-MBO at a higher Mn2+ concentration. Similarly, isoprene formation by Mn2+ increased exponentially as temperature increased with predominance of isoprene over 2-MBO at higher temperature. Both isoprene and 2-MBO formation was enhanced by acidic and neutral pH compared to alkaline conditions. Molecular dynamic simulation of DMADP suggested that the formation reaction is initiated by deprotonation of hydrogen on allyl terminal carbon by phosphate oxygen and generates carbocation and allyl anion intermediates. This is followed by quenching to produce isoprene or by hydroxyl addition to form 2-MBO. Thus, this study provided an insight into reaction mechanism of isoprene and 2-MBO biosynthesis and highlighted some parts of isoprene emission from terrestrial plants, which could be formed by non-enzymatic mechanism.

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Growth condition controls on G-93 parameters of isoprene emission from tropical trees.

Oku H, Iwai S, Uehara M, Iqbal A, Mutanda I, Inafuku M

Journal of plant research ( Journal of Plant Research )  134 ( 6 ) 1225 - 1242   2021年11月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

Despite its major role in global isoprene emission, information on the environmental control of isoprene emission from tropical trees has remained scarce. Thus, in this study, we examined the relationship between parameters of G-93 isoprene emission formula (CT1, CT2, and α), growth temperature and light intensity, photosynthesis (ɸ, Pmax), isoprene synthase (IspS) level, and 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway metabolites using sunlit and shaded leaves of four tropical trees. The results showed that the temperature dependence of isoprene emission from shaded leaves did not differ significantly from sunlit leaves. In contrast, there was a lower saturation irradiance in shaded leaves than in sunlit leaves, the same as temperate plants. The photosynthesis rate of shaded leaves showed lower saturation irradiance, similar to the light dependence of isoprene emission. In most cases, the concentration of MEP pathway metabolites was of lower tendency in shaded leaves versus in sunlit leaves, whereas no significant difference was noted in IspS level between sunlit and shaded leaves. Correlation analysis between these parameters found that CT1 of the G-93 parameter was positively correlated with the concentration of DXP and DMADP, whereas CT2 correlated with the concentration of MEP and the average air temperature for the past 48 h. Similarly, α closely associated with the initial slope (ɸ) of photosynthesis rate, and the basal emission factor is also linked to the photon flux of past days. These results suggest that growth conditions may control the temperature dependence of isoprene emission from tropical trees via the changes in the profiles of MEP pathway metabolites, causing alteration in the parameters of the isoprene emission formula.

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Mechanism of the fungal-like particles in the inhibition of adipogenesis in 3T3-L1 adipocytes.

Jakkawanpitak C, Inafuku M, Oku H, Hutadilok-Towatana N, Bunkrongcheap R, Sermwittayawong N, Aiemchareon P, Sermwittayawong D

Scientific reports ( Scientific Reports )  11 ( 1 ) 18869 - 18869   2021年09月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

The dynamic ability of adipocytes in adipose tissue to store lipid in response to changes in the nutritional input and inflammatory elicitors has a major impact on human health. Previously, we established laminarin-coated beads or LCB as an inflammatory elicitor for adipocytes. However, it was not clear whether LCB inhibits lipid accumulation in adipocytes. Here, we show that LCB acts in the early stage of adipogenesis through both interleukin-1 receptor-associated kinases (IRAK) and spleen tyrosine kinase (SYK) pathways, resulting in the activation of the AMP-activated protein kinase (AMPK) and nuclear factor-κB (NF-κB) complexes, which subsequently cause cell cycle arrest, downregulation of the key transcription factors and enzymes responsible for adipogenesis, inhibition of adipogenesis, and stimulation of an inflammatory response. While LCB could effectively block lipid accumulation during the early stage of adipogenesis, it could stimulate an inflammatory response at any stage of differentiation. Additionally, our results raise a possibility that toll-like receptor 2 (TLR2) and C-type lectin domain family 7 member A (CLEC7A/Dectin-1) might be potential β-glucan receptors on the fat cells. Together, we present the mechanism of LCB, as fungal-like particles, that elicits an inflammatory response and inhibits adipogenesis at the early stage of differentiation.

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

The dose-dependent pteryxin-mediated molecular mechanisms in suppressing adipogenesis in vitro

Nugara R.N.

Journal of Functional Foods ( Journal of Functional Foods )  82   2021年07月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

The aim was to perform an in-depth characterization of pteryxin in the anti-obesity related molecular mechanisms using 3T3-L1 preadipocytes. Cells were treated with pteryxin 20 (PTX20) or 50 (PTX50) μg/mL during cell differentiation or maintenance period. Pteryxin dose-dependently suppressed adipogenic gene network. PTX20 downregulated key lipogenic genes suppressing fatty acid synthesis allotting the anti-adipogenesis in the late adipocyte formation stage. PTX50 downregulated adipogenesis related genes, and accelerated lipolytic activity by repressing the adipocyte size marker gene, MEST. In addition to Wnt5a, PTX50 suppressed adipocyte differentiation through TGF-β/Smad3 signaling pathway with the maximum adiposity inhibition in the early stage of adipogenesis and regulated the carbohydrate metabolism. Pteryxin regulated phosphorylation of ERK1/2 and AMPK, and decreased p38 MAPK to repress the mature adipocyte formation. Overall, we have demonstrated the anti-adipogenic characteristics of pteryxin consistent to its dose. PTX50 may serve as a potential candidate to ameliorate adiposity and improve the carbohydrate metabolism.

• この論文にアクセスする

  DOI

Nitric Oxide Regulates Plant Growth, Physiology, Antioxidant Defense, and Ion Homeostasis to Confer Salt Tolerance in the Mangrove Species, <i>Kandelia obovata</i>.

Hasanuzzaman M, Inafuku M, Nahar K, Fujita M, Oku H

Antioxidants (Basel, Switzerland) ( Antioxidants )  10 ( 4 )   2021年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

Facultative halophyte Kandelia obovata plants were exposed to mild (1.5% NaCl) and severe (3% NaCl) salt stress with or without sodium nitroprusside (SNP; 100 µM; a NO donor), hemoglobin (Hb, 100 µM; a NO scavenger), or Nω-nitro-L-arginine methyl ester (L-NAME, 100 µM; a NO synthase inhibitor). The plants were significantly affected by severe salt stress. They showed decreases in seedling growth, stomatal conductance, intercellular CO2 concentration, SPAD value, photosynthetic rate, transpiration rate, water use efficiency, and disrupted antioxidant defense systems, overproduction of reactive oxygen species, and visible oxidative damage. Salt stress also induced ion toxicity and disrupted nutrient homeostasis, as indicated by elevated leaf and root Na+ contents, decreased K+ contents, lower K+/Na+ ratios, and decreased Ca contents while increasing osmolyte (proline) levels. Treatment of salt-stressed plants with SNP increased endogenous NO levels, reduced ion toxicity, and improved nutrient homeostasis while further increasing Pro levels to maintain osmotic balance. SNP treatment also improved gas exchange parameters and enhanced antioxidant enzymes’ activities (catalase, ascorbate peroxidase, monodehydroascorbate reductase, and dehydroascorbate reductase). Treatment with Hb and L-NAME reversed these beneficial SNP effects and exacerbated salt damage, confirming that SNP promoted stress recovery and improved plant growth under salt stress.

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Syringin: A phenylpropanoid glycoside compound in Cirsium brevicaule A. GRAY root modulates adipogenesis

Abu Yousuf Hossin, Masashi Inafuku, Kensaku Takara, Ruwani N. Nugara, Hirosuke Oku

Molecules   26 ( 6 )   2021年03月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

Cirsium brevicaule A. GRAY is a wild perennial herb, and its roots (CbR) have traditionally been used as both food and medicine on the Japanese islands of Okinawa and Amami. The present study evaluated the antiadipogenic effect of CbR using mouse embryonic fibroblast cell line 3T3-L1 from JCRB cell bank. Dried CbR powder was serially extracted with solvents of various polarities, and these crude extracts were tested for antiadipogenic activity. Treatment with the methanol extract of CbR showed a significant suppression of lipid accumulation in 3T3-L1 cells. Methanol extract of CbR was then fractionated and subjected to further activity analyses. The phenylpropanoid glycosidic molecule syringin was identified as an active compound. Syringin dose dependently suppressed lipid accumulation of 3T3-L1 cells without cytotoxicity, and significantly reduced the expressions of peroxisome proliferator-activated receptor gamma, the master regulator of adipogenesis, and other differentiation markers. It was demonstrated that syringin effectively enhanced the phosphorylation of the AMP-activated protein kinase and acetyl-CoA carboxylase. These results indicate that syringin attenuates adipocyte differentiation, adipogenesis, and promotes lipid metabolism; thus, syringin may potentially serve as a therapeutic candidate for treatment of obesity.

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Anticancer Effects of Polyisoprenoid From Nypa fruticans Leaves by Controlling Expression of p53, EGFR, PI3K, AKT1, and mTOR Genes in Colon Cancer (WiDr) Cells

Meighina Atika Istiqomah, Poppy Anjelisa, Zaitun Hasibuan, Sumaiyah Sumaiyah, Era Yusraini, Hirosuke Oku, Mohammad Basyuni

Natural Product Communications   15 ( 4 ) 1 - 8   2020年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

Molecular cloning of putative chloroplastic cysteine synthase in Leucaena leucocephala.

Harun-Ur-Rashid M, Oogai S, Parveen S, Inafuku M, Iwasaki H, Fukuta M, Amzad Hossain M, Oku H

Journal of plant research ( Journal of Plant Research )  133 ( 1 ) 95 - 108   2020年01月 [ 査読有り ]

掲載種別: 研究論文（その他学術会議資料等）

　概要を見る

Cysteine biosynthesis is directed by the successive commitments of serine acetyltransferase, and O-acetylserine (thiol) lyase (OASTL) compounds, which subsequently frame the decameric cysteine synthase complex. The isoforms of OASTL are found in three compartments of the cell: the cytosol, plastid, and mitochondria. In this investigation, we first isolated putative chloroplastic OASTL (Ch-OASTL) from Leucaena leucocephala, and the Ch-OASTL was then expressed in BL21-competent Escherichia coli. The putative Ch-OASTL cDNA clone had 1,543 base pairs with 391 amino acids in its open reading frame and a molecular weight of 41.54 kDa. The purified protein product exhibited cysteine synthesis ability, but not mimosine synthesis activity. However, they both make the common α-aminoacrylate intermediate in their first half reaction scheme with the conventional substrate O-acetyl serine (OAS). Hence, we considered putative Ch-OASTL a cysteine-specific enzyme. Kinetic studies demonstrated that the optimum pH for cysteine synthesis was 7.0, and the optimum temperature was 40 °C. In the cysteine synthesis assay, the Km and kcat values were 838 ± 26 µM and 72.83 s-1 for OAS, respectively, and 60 ± 2 µM and 2.43 s-1 for Na2S, respectively. We can infer that putative Ch-OASTL regulatory role is considered a sensor for sulfur constraint conditions, and it acts as a forerunner of various metabolic compound molecules.

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Dihydropyranocoumarins Exerted Anti-Obesity Activity In Vivo and its Activity Was Enhanced by Nanoparticulation with Polylactic-Co-Glycolic Acid.

Hossin AY, Inafuku M, Oku H

Nutrients ( Nutrients )  11 ( 12 )   2019年12月 [ 査読有り ]

掲載種別: 研究論文（その他学術会議資料等）

　概要を見る

Dihydropyranocoumarins (DPCs) were isolated from Peucedanumjaponicum Thunb as anti-obesity compounds in 3T3-L1 adipocytes assay; however, it is uncertain whether DPC exerts anti-obesity activity in vivo. Therefore, this study evaluated the oral intake of pure DPCs in mice fed a high-fat diet, and also attempted to enhance its activity by nanoparticulation. Increases in body weight gain and fat accumulation in white adipose tissues were significantly suppressed by the dietary intake of DPCs (1.943 mg/mouse/day). DPCs intake also significantly decreased the mean size of adipocytes and upregulated mRNA levels of thermogenesis-related genes. Nanoparticulation of DPCs with polylactic-co-glycolic acid (PLGA) dramatically increased its activity almost 100-fold over that of a non-nanoparticulated form. Thus, our findings clearly demonstrated the anti-obesity activity of DPCs in vivo and suggested that PLGA nanoparticle encapsulation was useful to enhance the anti-obesity activity of DPCs with the aim to develop natural and safe anti-obesity agents.

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Effect of purified soluble polysaccharides extracted from gray oyster mushroom [pleurotus sajor-caju (fr.) sing.] on 3t3-l1 adipocytes

Sermwittayawong D.

Sains Malaysiana ( Sains Malaysiana )  49 ( 1 ) 103 - 112   2019年10月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

© 2020 Penerbit Universiti Kebangsaan Malaysia. All rights reserved. Functional mushroom polysaccharides have been known to the Asian traditional medicine for many years. The anti-obesity activity of mushroom polysaccharides has been illustrated both in vivo and in vitro. However, the effect of pure polysaccharides on isolated fat cells has not been demonstrated. In this study, we used hot water and ammonium oxalate to extract polysaccharides from gray oyster mushroom [Pleurotus sajor-caju (Fr.) Sing.] and subsequently purified with enzymatic digestion, solvent extraction and ion-exchange chromatography. One of the fractions, 7S1-1, has the highest yield and purity and contains polysaccharides with the molecular weight around 500 kDa. In addition, Fourier transformed infrared spectroscopy (FTIR) and monosaccharide composition analyses showed that this 7S1-1 sample contains mostly β-glucan and some mannan. We find that the 7S1-1 fraction does not affect the total amount of triglycerides in the 3T3-L1 adipocytes but promotes a release of glycerol of the fat cells, suggesting that the fraction does not inhibit adipogenesis but stimulates lipolysis. Laminarin, a small β-glucan, confers similar results to the 7S1-1 sample, but it could not stimulate lipolysis as well as the 7S1-1 sample. Therefore, these results suggest a potential anti-obesity activity of the mushroom polysaccharides.

• この論文にアクセスする

  DOI

Molecular characterization of mimosinase and cystathionine β-lyase in the Mimosoideae subfamily member Mimosa pudica.

Oogai S, Fukuta M, Watanabe K, Inafuku M, Oku H

Journal of plant research ( Journal of Plant Research )  132 ( 5 ) 667 - 680   2019年09月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Plant hormone effects on isoprene emission from tropical tree in Ficus septica.

Parveen S, Iqbal MA, Mutanda I, Rashid MH, Inafuku M, Oku H

Plant, cell & environment ( Plant Cell and Environment )  42 ( 5 ) 1715 - 1728   2019年05月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Molecular regulatory mechanism of isoprene emission under short-term drought stress in the tropical tree Ficus septica.

Parveen S, Rashid MH, Inafuku M, Iwasaki H, Oku H

Tree physiology ( Tree Physiology )  39 ( 3 ) 440 - 453   2019年03月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

Isoprene is emitted by many plants and is thought to function as an antioxidant under stressful conditions. However, the detailed regulatory mechanism of isoprene emission in relation to the antioxidant system remains unclear. Therefore, in this study, we explored the molecular regulatory mechanism of isoprene emission under short-term drought stress in the tropical tree Ficus septica Burm.f. We found that the soil moisture content gradually decreased from 55% on Day 1 (D1) to 23% (wilting point) on D5 after withholding water for 4 days and then returning to the initial level following re-watering on D6. On D5, drought-stressed plants had more than twofold higher isoprene emission and 90.6% lower photosynthesis rates, 99.5% lower stomatal conductance and 82.3% lower transpiration rates than well-watered control plants. It was also estimated that the isoprene concentration inside the leaf greatly increased on D5 due to the increased isoprene emission rate and reduced stomatal conductance. Among the traits related to the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway, which is responsible for isoprene biosynthesis, the isoprene synthase (IspS) protein level was positively correlated with the isoprene emission rate in stressed plants. The transcripts of the antioxidant genes peroxidase 2 (POD2), POD4, copper-zinc superoxide dismutase 2 (Cu-ZnSOD2) and manganese superoxide dismutase 1 (Mn-SOD1) also increased during the drying period, while those of ascorbate peroxidase 1 (APX1) decreased. However, there was only a weak correlation between isoprene emission and antioxidant enzyme gene expression, indicating that the regulation of isoprene biosynthesis is not directly linked to the antioxidant defense network in drought-stressed F. septica. These findings suggest that the post-transcriptional regulation of IspS led to the observed change in isoprene emission rate, which enhanced the quenching of reactive oxygen species (ROS) and, in combination with the increased antioxidant enzyme activity, conferred tolerance to drought stress in this species.

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Inhibition of Niemann-Pick C1-Like 1 by Ezetimibe Reduces Dietary 5β,6β-Epoxycholesterol Absorption in Rats.

Shirouchi B, Furukawa Y, Nakamura Y, Kawauchi A, Imaizumi K, Oku H, Sato M

Cardiovascular drugs and therapy ( Cardiovascular Drugs and Therapy )  33 ( 1 ) 35 - 44   2019年02月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

© 2019, Springer Science+Business Media, LLC, part of Springer Nature. Purpose: Oxycholesterols (OCs) are produced from cholesterol by oxidation of the steroidal backbone and side-chain. OCs are present in blood and evidence suggests their involvement in disease development and progression. However, limited information is available regarding the absorption mechanisms and relative absorption rates of dietary OCs. Although ezetimibe is known to inhibit intestinal cholesterol absorption via Niemann-Pick C1-Like 1 (NPC1L1), whether it also inhibits dietary OC absorption is unclear. Methods: We investigated the effects of ezetimibe on OC absorption in rats fed an OC-rich diet containing 10 different OCs. We collected lymphatic fluid using permanent cannulation of the thoracic duct and quantified OC levels. Results: Ezetimibe treatment significantly reduced the apparent absorption of 5β,6β-epoxycholesterol (5,6β-epoxy) and its levels in the proximal intestinal mucosa in OC-fed rats. Using in silico analyses, the binding energy of NPC1L1 N-terminal domain (NPC1L1-NTD) and 5,6β-epoxy was found to be similar to that of NPC1L1-NTD and cholesterol, suggesting that polar uncharged amino acids located in the steroidal part of 5,6β-epoxy were involved. Conclusion: Our results indicate that ezetimibe-mediated inhibition of dietary OC absorption varies depending on the specific OC, and only the absorption of 5,6β-epoxy is significantly reduced.

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Evaluation of isoprene emission rates of tropical trees by an iterative optimization procedure for G-93 parameters

Higa T.

Atmospheric Environment ( Atmospheric Environment )  192   209 - 217   2018年11月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Evaluation of isoprene emission rates of tropical trees by an iterative optimization procedure for G-93 parameters

TakuyaHiga, Shahanaz Parveen, Ishmael Mutand, Md AsifI qbal, Masashi Inafuku, Fumio Hashimoto, Hirosuke Oku

Atmospheric Environment   192   209 - 217   2018年11月

掲載種別: 研究論文（学術雑誌）

Exogenous nitric oxide donor and arginine provide protection against short-term drought stress in wheat seedlings.

Hasanuzzaman M, Nahar K, Rahman A, Inafuku M, Oku H, Fujita M

Physiology and molecular biology of plants : an international journal of functional plant biology ( Physiology and Molecular Biology of Plants )  24 ( 6 ) 993 - 1004   2018年11月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

Nitric oxide (NO) is an important plant signaling molecule that has a vital role in abiotic stress tolerance. In the present study, we assessed drought-induced (15 and 30% PEG, polyethylene glycol) damage in wheat (Triticum aestivum L. cv. Prodip) seedlings and mitigation by the synergistic effect of exogenous Arg (0.5 mM l-Arginine) and an NO donor (0.5 mM sodium nitroprusside, SNP). Drought stress sharply decreased the leaf relative water content (RWC) but markedly increased the proline (Pro) content in wheat seedlings. Drought stress caused overproduction of reactive oxygen species (ROS) and methylglyoxal (MG) due to the inefficiency of antioxidant enzymes, the glyoxalase system, and the ascorbate-glutathione pool. However, supplementation with the NO donor and Arg enhanced the antioxidant defense system (both non-enzymatic and enzymatic components) in drought-stressed seedlings. Application of the NO donor and Arg also enhanced the glyoxalase system and reduced the MG content by increasing the activities of the glyoxalase system enzymes (Gly I and Gly II), which restored the leaf RWC and further increased the Pro content under drought stress conditions. Exogenous NO donor and Arg application enhanced the endogenous NO content, which positively regulated the antioxidant system and reduced ROS production. Thus, the present study reveals the crucial roles of Arg and NO in enhancing drought stress tolerance in wheat seedlings by upgrading their water status and reducing oxidative stress and MG toxicity.

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Microsatellite analysis of the mating system of Bruguiera gymnorrhiza and Kandelia obovata

Basyuni M.

MATEC Web of Conferences ( MATEC Web of Conferences )  197   2018年09月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Bioinformatics analysis of the expressed sequence tags from Rhizophora stylosa Griff. genomic library

Wati R.

AIP Conference Proceedings ( AIP Conference Proceedings )  2002   2018年08月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Isolation and phylogenetic analysis of new predicted polyprenol reductase from mangrove plant (Kandelia obovata Sheue, H. Y. Liu &amp; J. Yong)

Basyuni M.

AIP Conference Proceedings ( AIP Conference Proceedings )  2002   2018年08月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Characterization of cycloartenol synthase KcCAS promoter region from mangrove plant (Kandelia obovata Sheue, H. Y. Liu &amp; J. Yong)

Basyuni M.

AIP Conference Proceedings ( AIP Conference Proceedings )  2002   2018年08月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Exogenous nitric oxide pretreatment protects Brassica napus L. seedlings from paraquat toxicity through the modulation of antioxidant defense and glyoxalase systems.

Hasanuzzaman M, Nahar K, Alam MM, Bhuyan MHMB, Oku H, Fujita M

Plant physiology and biochemistry : PPB ( Plant Physiology and Biochemistry )  126   173 - 186   2018年05月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Cytosolic Cysteine Synthase Switch Cysteine and Mimosine Production in Leucaena leucocephala.

Md Harun-Ur-Rashid, Iwasaki H, Parveen S, Oogai S, Fukuta M, Hossain MA, Anai T, Oku H

Applied biochemistry and biotechnology     2018年04月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Nitric oxide-induced salt stress tolerance in plants: ROS metabolism, signaling, and molecular interactions

Hasanuzzaman M.

Plant Biotechnology Reports ( Plant Biotechnology Reports )  12 ( 2 ) 77 - 92   2018年04月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Genome sequence analysis of predicted polyprenol reductase gene from mangrove plant kandelia obovata

Basyuni M.

IOP Conference Series: Earth and Environmental Science ( IOP Conference Series: Earth and Environmental Science )  130 ( 1 )   2018年03月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Isolation and analysis of a multifunctional triterpene synthase KcMS promoter region from mangrove plant kandelia candel

Basyuni M.

IOP Conference Series: Earth and Environmental Science ( IOP Conference Series: Earth and Environmental Science )  130 ( 1 )   2018年03月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Bioinformatics approach of three partial polyprenol reductase genes in Kandelia obovata

Basyuni M.

Journal of Physics: Conference Series ( Journal of Physics: Conference Series )  978 ( 1 )   2018年03月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Predicted cycloartenol synthase protein from Kandelia obovata and Rhizophora stylosa using online software of Phyre2 and Swiss-model

Basyuni M.

Journal of Physics: Conference Series ( Journal of Physics: Conference Series )  978 ( 1 )   2018年03月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Protein modelling of triterpene synthase genes from mangrove plants using Phyre2 and Swiss-model

Basyuni M.

Journal of Physics: Conference Series ( Journal of Physics: Conference Series )  978 ( 1 )   2018年03月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Molecular characterization of cytosolic cysteine synthase in Mimosa pudica.

Rashid MH, Iwasaki H, Oogai S, Fukuta M, Parveen S, Hossain MA, Anai T, Oku H

Journal of plant research ( Journal of Plant Research )  131 ( 2 ) 319 - 329   2018年03月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Diversity and abundance of polyisoprenoid composition in coastal plant species from North Sumatra, Indonesia

Basyuni M.

Biodiversitas ( Biodiversitas )  19 ( 1 ) 1 - 11   2018年01月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Triterpenoid modulates the salt tolerance of lanosterol synthase deficient <i>Saccharomyces cerevisiae</i>, GIL77.

Inafuku M, Basyuni M, Oku H

Saudi journal of biological sciences ( Saudi Journal of Biological Sciences )  25 ( 1 ) 1 - 9   2018年01月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Differential enzymatic defense mechanisms in leaves and roots of two true mangrove species under long- term salt stress

Hossain M.

Aquatic Botany ( Aquatic Botany )  142   32 - 40   2017年09月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Antitumor effects of nitidine on camptothecin-resistant A549 cells in xenograft mouse model

Taira N, Inafuku M, lly contributed author, Oku H

Traditional & Kampo Medicine   4 ( 1 ) 19 - 25   2017年09月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Responses, adaptation, and ROS metabolism in plants exposed to waterlogging stress

Hasanuzzaman M.

Reactive Oxygen Species and Antioxidant Systems in Plants: Role and Regulation under Abiotic Stress ( Reactive Oxygen Species and Antioxidant Systems in Plants: Role and Regulation under Abiotic Stress )    257 - 281   2017年08月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Cloning and Phylogenetic Analysis of Actin Genes from Mangrove Family Rhizophoraceae

Basyuni M.

IOP Conference Series: Materials Science and Engineering ( IOP Conference Series: Materials Science and Engineering )  180 ( 1 )   2017年03月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Microsatellite Analysis on Genetic Variation in Two Populations of Red Mangrove Rhizophora Mangle L. (Rhizophoraceae) and Its Implication to Conservation

Basyuni M.

IOP Conference Series: Materials Science and Engineering ( IOP Conference Series: Materials Science and Engineering )  180 ( 1 )   2017年03月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

In vivo and in vitro anti-obesity activities of dihydropyranocoumarins derivatives from Peucedanum japonicum Thunb

Taira N.

Journal of Functional Foods ( Journal of Functional Foods )  29   19 - 28   2017年02月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Coordinated Actions of Glyoxalase and Antioxidant Defense Systems in Conferring Abiotic Stress Tolerance in Plants.

Hasanuzzaman M, Nahar K, Hossain MS, Mahmud JA, Rahman A, Inafuku M, Oku H, Fujita M

International journal of molecular sciences ( International Journal of Molecular Sciences )  18 ( 1 )   2017年01月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Distribution, occurrence, and cluster analysis of new polyprenyl acetones and other polyisoprenoids from North Sumatran mangroves

Basyuni M.

Dendrobiology ( Dendrobiology )  78   18 - 31   2017年01月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Salinity Alters the Polyisoprenoid Alcohol Content and Composition of Both Salt-Secreting and Non–Salt-Secreting Mangrove Seedlings

Basyuni M.

HAYATI Journal of Biosciences ( HAYATI Journal of Biosciences )  24 ( 4 ) 206 - 214   2017年01月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Tumor-Selective Cytotoxicity of Nitidine Results from Its Rapid Accumulation into Mitochondria.

Iwasaki H, Inafuku M, Taira N, Saito S, Oku H

BioMed research international ( BioMed Research International )  2017   2130594   2017年

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Lipid-lowering effects of hexane fraction of ivy gourd (Coccinia grandis L. voigt) root in mice fed a high-fat diet

Bunkrongcheap R.

Walailak Journal of Science and Technology ( Walailak Journal of Science and Technology )  13 ( 10Special Issue ) 815 - 825   2016年10月

掲載種別: 研究論文（学術雑誌）

Temperature controls on the basal emission rate of isoprene in a tropical tree Ficus septica: exploring molecular regulatory mechanisms.

Mutanda I, Inafuku M, Saitoh S, Iwasaki H, Fukuta M, Watanabe K, Oku H

Plant, cell & environment ( Plant Cell and Environment )  39 ( 10 ) 2260 - 75   2016年10月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Lipid-lowering effects of hexane fraction of Ivy Gourd (Coccinia grandis L. Voigt) root in mice fed a high-fat diet

Bunkrongcheap R, Inafuku M, Oku H, Hutadilok-Towatana N, Wattanapiromsakul C. Sermwittayawong D

Walailak Journal of Science and Technology   13 ( 10 ) 815 - 825   2016年10月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

Parameterization of G-93 isoprene emission formula for tropical trees Casuarina equisetifolia and Ficus septica

Mutanda I.

Atmospheric Environment ( Atmospheric Environment )  141   287 - 296   2016年09月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Extraction method influenced physical, aroma, and antioxidant profiles of Shiikuwasha (Citrus depressa Hayata) pulp essential oil

Asikin Y.

Acta Horticulturae ( Acta Horticulturae )  1135   61 - 72   2016年07月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Gene expression analysis of disabled and re-induced isoprene emission by the tropical tree Ficus septica before and after cold ambient temperature exposure.

Mutanda I, Saitoh S, Inafuku M, Aoyama H, Takamine T, Satou K, Akutsu M, Teruya K, Tamotsu H, Shimoji M, Sunagawa H, Oku H

Tree physiology ( Tree Physiology )  36 ( 7 ) 873 - 82   2016年07月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Monogalactosyldiacylglycerol: An abundant galactosyllipid of Cirsium brevicaule A. GRAY leaves inhibits the expression of gene encoding fatty acid synthase.

Inafuku M, Takara K, Taira N, Nugara RN, Kamiyama Y, Oku H

Phytomedicine : international journal of phytotherapy and phytopharmacology ( Phytomedicine )  23 ( 5 ) 509 - 16   2016年05月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Studies on the enhancement of cancer-selective cytotoxicity of herbal medicine by combination

Susanti S.

International Journal of Pharmacognosy and Phytochemical Research ( International Journal of Pharmacognosy and Phytochemical Research )  8 ( 5 ) 841 - 847   2016年05月

掲載種別: 研究論文（学術雑誌）

DNA damage protection against free radicals of two antioxidant neolignan glucosides from sugarcane molasses.

Asikin Y, Takahashi M, Mizu M, Takara K, Oku H, Wada K

Journal of the science of food and agriculture ( Journal of the Science of Food and Agriculture )  96 ( 4 ) 1209 - 15   2016年03月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Effects of different drying-solidification processes on physical properties, volatile fraction, and antioxidant activity of non-centrifugal cane brown sugar

Asikin Y.

LWT - Food Science and Technology ( LWT - Food Science and Technology )  66   340 - 347   2016年03月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Diversity of polyisoprenoids in ten Okinawan mangroves

Basyuni M.

Dendrobiology ( Dendrobiology )  75   167 - 175   2016年01月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

熱帯樹木のイソプレン放出調節機構の解明とその応用 (特集 油化学関連技術を用いた亜熱帯生物資源の利活用)

屋 宏典, 稲福 征志, 齋藤 星耕

オレオサイエンス ( 日本油化学会 )  16 ( 1 ) 7 - 19,4-5   2016年01月

掲載種別: 研究論文（学術雑誌）

Peucedanum japonicum Thunb and its Antiobesity Effects: Evidence and Related Mechanisms

Nugara Ruwani N., Inafuku Masashi, Oku Hirosuke

脂質栄養学 ( 日本脂質栄養学会 )  25 ( 2 ) 177 - 196   2016年

掲載種別: 研究論文（学術雑誌）

　概要を見る

Lifestyle modification is the main treatment for obesity. An understanding of the mechanisms of appetite control, nutrient metabolism, and energy expenditure has greatly enhanced drug development for disorders, such as diabetes, cancer, obesity, and inflammation. Peucedanum japonicum Thunb (PJT), also known as "Chomeiso" (長命草), is a traditional herb that has been used to provide nutrition; the herb has a broad spectrum of physiological and biological properties. In our previous studies, we found that PJT has antiobesity effects, more specifically, the ethanol extract of PJT reduces obesity and diabetes symptoms in high-fat diet-fed mice. Furthermore, in one of our recent studies, we isolated the active component of PJT with antiobesity properties called pteryxin, a previously known coumarin. However, its biological properties against obesity were unknown. We determined that pteryxin played a role in adipogenesis suppression mechanisms, both in vitro and in vivo. This study details the current understanding of antiobesity effects of PJT extracts, its active compound pteryxin, and the distinctive properties of pteryxin compared with those of other natural compounds against obesity. This review will help in understanding the molecular mechanisms underlying pteryxin's effects for development of pharmaceutical drugs and nutritional products.

• この論文にアクセスする

  DOI

Diversity of polyisoprenoids in ten Okinawan mangroves

Basyuni Mohammad, Sagami Hiroshi, Baba Shigeyuki, Iwasaki Hironori, Oku Hirosuke

DENDROBIOLOGY   75   167 - 175   2016年

掲載種別: 研究論文（学術雑誌）

黒麹・乳酸菌飼料によるブロイラーの盲腸内短鎖脂肪酸の変動

日置久美子, 日置久美子, 川崎千穂子, 山元正博, 林国興, 屋宏典

日本家禽学会誌   52 ( J2 ) J48-J55   2015年10月

掲載種別: 研究論文（学術雑誌）

• 関連情報を調べる
  J-GLOBAL

Molecular cloning and biochemical characterization of isoprene synthases from the tropical trees Ficus virgata, Ficus septica, and Casuarina equisetifolia.

Oku H, Inafuku M, Ishikawa T, Takamine T, Ishmael M, Fukuta M

Journal of plant research ( Journal of Plant Research )  128 ( 5 ) 849 - 61   2015年09月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

Cultivation line and fruit ripening discriminations of Shiikuwasha (Citrus depressa Hayata) peel oils using aroma compositional, electronic nose, and antioxidant analyses

Asikin Y.

Food Research International ( Food Research International )  67   102 - 110   2015年01月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Intravenous Mycobacterium Bovis Bacillus Calmette-Guérin Ameliorates Nonalcoholic Fatty Liver Disease in Obese, Diabetic ob/ob Mice.

Inafuku M, Matsuzaki G, Oku H

PloS one ( PLoS ONE )  10 ( 6 ) e0128676   2015年

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI
• 関連情報を調べる
  PubMed

麹飼料による肥育豚の生産性向上

日置 久美子, 川崎 千穂子, 山元 正博, 林 国興, 屋 宏典

日暖畜報 ( 日本暖地畜産学会 )  58 ( 1 ) 45 - 53   2015年

掲載種別: 研究論文（学術雑誌）

　概要を見る

本研究では黒麹および黒麹リキッドフィード(LF) 給与が肥育豚の生産性に与える影響を調べた。実験1 では鹿児島バークシャーを用い、市販配合飼料に黒麹0.05％および0.1％を配合して給与した。その結果、無添加対照区に比べ黒麹0.05％区において増体量は改善傾向にあり飼料要求率（FCR）は有意に低下した。実験2 では三元交雑種（LW・D）去勢雄に乾物として配合飼料の20％および40％をLF（食品残さを黒麹で発酵させた）で代替して与えた。その結果、対照区（配合飼料区）に比べ20％給与区で増体量は改善されFCRは低下した。実験3 では、実験2 に準じ、配合飼料の20％を2 種のLF（黒麹と2 種の乳酸菌で調製した）で代替して与えた。その結果、Lactobacillus casei で調製したLF 給与区において、増体量およびFCR が顕著に改善された。以上のことから、黒麹を利用して栄養価の高いLF を作ることができ、黒麹給与により肥育豚の生産性が向上することが示された。

沖縄産マングローブの耐塩性に関与する細胞膜構成物質の検索

馬場　繁幸,

研究報告書（遺伝子実験センター） ( 屋宏典 )    2009年12月

掲載種別: 研究論文（その他学術会議資料等）

　概要を見る

平成15年度～16年度科学研究費補助金(基盤研究(C)(2))研究成果報告書 （管理者追加）リポジトリ登録情報を移行しました。確認のうえ、加除修正をしてください。

• この論文にアクセスする

  DOI

亜熱帯沖縄産樹木からのイソプレンガス放出の環境特性と調節メカニズムの解明

その他の著者

研究報告書（遺伝子実験センター） ( 屋宏典 )    2009年12月

掲載種別: 研究論文（その他学術会議資料等）

　概要を見る

平成17年度～平成18年度科学研究費補助金(基盤研究(C))研究成果報告書 （管理者追加）リポジトリ登録情報を移行しました。確認のうえ、加除修正をしてください。

• この論文にアクセスする

  DOI

Characterization and bioavailability of liposomes containing a Ukon extract

Takahashi Makoto, Dai Kitamoto, Tomohiro Imura, Hirosuke Oku, Kensaku Takara and Koji Wada

Biosci. Biotechnol. Biochem ( その他の出版社 )  72   1199 - 1205   2008年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Occurrence of HR1b in the venom of Okinawa habu (Protobothrops flavoviridis)

Nobuya MORINE, Seiko MATSUDA, Koki TERADA, Hironori IWASAKI, and Hirosuke OKU

Biosci Biotechnol Biochem ( その他の出版社 )  72   591 - 594   2008年03月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

Neutralization of hemorrhagic snake venom metalloproteinase HR1a from Protobothrops flavoviridis by human monoclonal antibody

Nobuya Morine, Seiko Matsuda, Koki Terada, Akira Eto, Isao Ishida, Hirosuke Oku

Toxicon ( その他の出版社 )  51   345 - 352   2008年02月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Triterpene synthases from the Okinawan mangrove tribe,Rhizophoraceae

Mohammad Basyuni, Hirosuke Oku, Etsuko Tsujimoto, Kazuhiko Kinjo, Shigeyuki Baba and Kensaku Takara

FEBS Journal ( その他の出版社 )  274   5028 - 5042   2007年07月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Cloning and Functional Expression of Cycloartenol Synthases from Mangrove Species Rhizophora stylosa Griff. and Kandelia candel (L.) Druce

Mohammad Basyuni, Hirosuke Oku, Etsuko Tsujimoto,and Shigeyuki Baba

Biosci. Biotech. Biochem. ( その他の出版社 )  71   1788 - 1792   2007年06月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Expression of Cell-Cycle-Regulating Genes in the Development of Atherosclerosis in Japanese Quail (Coturnix japonica)

Inafuku M, Toda T, Okabe T, Shinjo A, Iwasaki H, Oku H.

Poult. Sci. ( その他の出版社 )  86   1166 - 1173   2007年05月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

Effect of Kokuto, a non-centrifugal cane sugar, on the development of experimental atherosclerosis in Japanese quails and apolipoprotein E deficient mice

Masashi Inafuku, Takayoshi Toda, Takafumi Okabe, Koji Wada, Kensaku Takara, Hironori Iwasaki, Hirosuke Oku

Food Science and Technology Research ( その他の出版社 )  13   61 - 66   2007年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

Isoprenoids of Okinawan Mangroves as Lipid Input into Estuarine Ecosystem

Mohammad Basyuni, Hirosuke Oku, Shigeyuki Baba, Kensaku Takara, Hironori Iwasaki

J Oceanography ( その他の出版社 )  63   601 - 608   2007年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

Tumor specific sytotoxicity of glucosylceramide

H. Oku, S. Wongtangtintharn, H. Iwasaki, M. Shimatani, T. Toda

Cancer Chemother. Pharmacol. ( その他の出版社 )    2007年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Efficient preparation of liposomes encapsulating food materials using lecithins by a mechanochemical method

Makoto TAKAHASHI, Kei-ichiro INAFUKU, Takeshi MIYAGI, Hirosuke OKU, Koji WADA, Tomohiro IMURA and Dai KITAMOTO

Journal of Oleo Science ( その他の出版社 )  56   35 - 42   2007年02月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

Effect of humidity on isoprene emission from a leaf of tropical tree Ficus virgata

Parlindungan Tambunan, Shigeyuki Baba, Hirosuke Oku

Japanese Journal of Tropical Agriculture ( その他の出版社 )  51   30 - 34   2007年02月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

ホソバワダン(Crepidiastrum lanceolatum)のLDL抗酸化成分とラットにおける血中動態

前田剛希，広瀬直人，屋宏典，高良健作，和田浩二

日本食品科学工学会誌 ( その他の出版社 )  53   627 - 633   2006年11月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Molecular cloning and functional expression of a multifunctional triterpene synthase cDNA from a mangrove species Kandelia candel (L.) Druce

Mohammad Basyuni, Hirosuke Oku, Masashi Inafuku, Shigeyuki Baba, Hironori Iwasaki, Keichiro Oshiro, Takafumi Okabe, Masaaki Shibuya, Yutaka Ebizuka

Phytochemistry ( その他の出版社 )  67   2517 - 2524   2006年05月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

乳ガン細胞への細胞毒性に及ぼす脂肪酸の炭素鎖構造の影響

S. Wongtangtintharn, 屋　宏典、稲福　征志、岩崎公典、戸田隆義

日本栄養・食糧学会誌 ( その他の出版社 )  59   115 - 118   2006年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

Isoprene emission from tropical trees in Okinawa Island, Japan

P. Tambunan, S. Baba, A. Kuniyosh, H. Iwasak, T. Nakamura, H.Yamasaki and H. Oku

Chemosphere ( その他の出版社 )  65   2138 - 2144   2006年04月

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

The tumor specific cytotoxicity of dihydronitidine form Todaria asiatica Lam

H. Iwasaki, H. Oku, R. Takara, H. Miyahira, K. Hanashiro, Y. Yoshida, Y. Kamada, T. Toyokawa, K. Takara, M. Inafuku

Cancer Chemother Pharmacol ( その他の出版社 )  2006   2006年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Incorporation of branched-chain fatty acid into cellular lipids and caspase-independent apoptosis in human breast cancer cell line, SKBR-3

S. Wongtangtintharn, H. Oku, H. Iwasaki, M. Inafuku, T. Toda, and T. Yanagita

Lipids in Health and Disease ( その他の出版社 )  4   29   2005年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

• この論文にアクセスする

  DOI

Effect of bleaching on lipid content and composition of Okinawan corals

H.Yamashiro et al.

Fisheries Sci ( その他の出版社 )  ( 71 ) 448 - 453   2005年03月

掲載種別: 研究論文（その他学術会議資料等）

• この論文にアクセスする

  DOI

Effect of branched-chain fatty acids on fatty acid biosynthesis of human breast cnacer cells.

S.Wongtangtintharn et al.

J. Nutr. Sci. Vitaminol ( その他の出版社 )  ( 50 ) 137 - 143   2004年03月

掲載種別: 研究論文（その他学術会議資料等）

Analysis of microsatellite instability and loss of heterozygosity in human aortic atherosclerotic lesions.

M.Inafuku et al.

Rinshobyouri ( その他の出版社 )  ( 52 ) 961 - 965   2004年03月

掲載種別: 研究論文（その他学術会議資料等）

Lipid composition of mangrove and its relevance to salt tolerance

Oku.H et al.

J. Plant Res ( その他の出版社 )  ( 116 ) 37 - 45   2003年03月

掲載種別: 研究論文（その他学術会議資料等）

• この論文にアクセスする

  DOI

Biosynthesis of branched-chain fatty acid in Bacilli: FabD (malonyl-CoA: ACP transacylase) is not essential for in vitro biosynthesis of branched-chain fatty acids.

Oku.H et al.

Biosci. Biotech. Biochem ( その他の出版社 )  ( 67 ) 2106 - 2114   2003年03月

掲載種別: 研究論文（その他学術会議資料等）

Conjugated linoleic acid (CLA) inhibits fatty acid synthetase activity in vitro.

Oku.H et al.

Biosci. Biotech. Biochem ( その他の出版社 )  ( 67 ) 1548 - 1586   2003年03月

掲載種別: 研究論文（その他学術会議資料等）

Seasonal changes in the content and composition of lipids in the coral Goniastrea aspera.

Oku.H et al.

Coral Reefs ( その他の出版社 )  ( 22 ) 83 - 85   2003年03月

掲載種別: 研究論文（その他学術会議資料等）

Lipid biosynthesis from [14C]-glucose in the coral Montipora digitata

Oku.H et al.

Fisheries Sci ( その他の出版社 )  ( 69 ) 625 - 631   2003年03月

掲載種別: 研究論文（その他学術会議資料等）

Lipid composition of mangrove and its relevance to salt tolerance

Oku.H et al.

J. Plant Res ( その他の出版社 )  ( 116 ) 37 - 45   2003年03月

掲載種別: 研究論文（その他学術会議資料等）

• この論文にアクセスする

  DOI

Biosynthesis of branched-chain fatty acid in Bacilli: FabD (malonyl-CoA: ACP transacylase) is not essential for in vitro biosynthesis of branched-chain fatty acids.

Oku.H et al.

Biosci. Biotech. Biochem. ( その他の出版社 )  ( 67 ) 2106 - 2114   2003年03月

掲載種別: 研究論文（その他学術会議資料等）

• この論文にアクセスする

  DOI

Conjugated linoleic acid (CLA) inhibits fatty acid synthetase activity in vitro.

Oku.H et al.

Biosci. Biotech. Biochem ( その他の出版社 )  ( 67 ) 1584 - 1586   2003年03月

掲載種別: 研究論文（その他学術会議資料等）

• この論文にアクセスする

  DOI

Seasonal changes in the content and composition of lipids in the coral Goniastrea aspera.

Oku.H et al.

Coral Reefs ( その他の出版社 )  ( 22 ) 83 - 85   2003年03月

掲載種別: 研究論文（その他学術会議資料等）

Lipid biosynthesis from [14C]-glucose in the coral Montipora digitata

Oku.H et al.

Fisheries Sci ( その他の出版社 )  ( 69 ) 625 - 631   2003年03月

掲載種別: 研究論文（その他学術会議資料等）

• この論文にアクセスする

  DOI

Lipid distribution in branching coral Montipora digitata.

Oku.H et al.

Fisｈeries Sci ( その他の出版社 )  ( 68 ) 517 - 522   2002年03月

掲載種別: 研究論文（その他学術会議資料等）

A male and hermaphrodite specific RAPD marker for papaya (Carica papaya L.)

Urasaki.N et al.

Theor. Appl. Genet ( その他の出版社 )  ( 104 ) 281 - 285   2002年03月

掲載種別: 研究論文（その他学術会議資料等）

• この論文にアクセスする

  DOI

Lipid distribution in branching coral Montipora digitata

Oku.H et al.

Fisｈeries Sci ( その他の出版社 )  ( 68 ) 517 - 522   2002年03月

掲載種別: 研究論文（その他学術会議資料等）

• この論文にアクセスする

  DOI

A male and hermaphrodite specific RAPD marker for papaya (Carica papaya L.).

Urasaki.N et al.

Theor. Appl. Genet ( その他の出版社 )  ( 104 ) 281 - 285   2002年03月

掲載種別: 研究論文（その他学術会議資料等）

Analysis of microsatellite instability and loss of heterozygosity in uterine endometrial Adenocarcinoma.

Toda.T et al.

Cancer Genet. Cytogenet ( その他の出版社 )  ( 126 ) 1 - 8   2001年03月

掲載種別: 研究論文（その他学術会議資料等）

• この論文にアクセスする

  DOI

Coral tumors store reduced levels of lipids

Yamashiro.H et al.

J. Exp. Mar. Biol. Ecol ( その他の出版社 )  ( 265 ) 171 - 179   2001年03月

掲載種別: 研究論文（その他学術会議資料等）

Analysis of microsatellite instability and loss of heterozygosity in uterine endometrial Adenocarcinoma.

Toda.T et al.

その他の掲載誌 ( その他の出版社 )  ( 126 ) 1 - 8   2001年03月

掲載種別: 研究論文（その他学術会議資料等）

Coral tumors store reduced level of lipids

Yamashiro.H et al.

J. Exp. Mar. Biol. Ecol ( その他の出版社 )  ( 265 ) 171 - 179   2001年03月

掲載種別: 研究論文（その他学術会議資料等）

• この論文にアクセスする

  DOI

Increased cholesterol absorption by hyperlipidemia atherosclerosis prone (LAP) Japnese quail.

Iwasaki.H et al.

J. Nutr. Sci. Vitaminol. ( その他の出版社 )  ( 46 ) 235 - 239   2000年03月

掲載種別: 研究論文（その他学術会議資料等）

Soybean-milk-coagulating activity of Bacillus pumilus derives from a serine protease.

Aoyama.M et al.

Appl. Microbiol. Biotech ( その他の出版社 )  ( 53 ) 390 - 395   2000年03月

掲載種別: 研究論文（その他学術会議資料等）

• この論文にアクセスする

  DOI

Biased distribution of the branched-chain fatty acids in vernix caseosa ceramides

Oku.H et al.

Lipids ( その他の出版社 )  ( 35 ) 373 - 381   2000年03月

掲載種別: 研究論文（その他学術会議資料等）

The major low molecular weight apolipoprotein from normal and hyperlipidemia atherosclerosis-prone (LAP) Japanese quail

Iwasaki.H et al.

Biochim. Biophys. Acta ( その他の出版社 )  ( 1483 ) 316 - 324   2000年03月

掲載種別: 研究論文（その他学術会議資料等）

• この論文にアクセスする

  DOI

Composition of lipids, fatty acids and sterols in Okinawan corals.

Yamashiro.H et al.

Comp. Biochem. Physiol ( その他の出版社 )  part B ( 122 ) 397 - 407   1999年03月

掲載種別: 研究論文（その他学術会議資料等）

• この論文にアクセスする

  DOI

Apolipoprotein A-I of hyperlipidemia athrrosclerosis prone (LAP) quail: cDNA sequence and tissue expression

Iwasaki.H et al.

Biosci. Biotech. Biochem ( その他の出版社 )  ( 63 ) 29 - 34   1999年03月

掲載種別: 研究論文（その他学術会議資料等）