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Published Papers 【 display / non-display 】

Published Papers 【 display / non-display 】

• Combination of combined-culture of <i>Amycolatopsis</i> sp. with <i>Tsukamurella pulmonis</i> and GNPS molecular networking reveals novel sulfur-containing cyclic lipopeptides thioamycolamides F-I

  Pan, CQ; Zhang, L; Kuranaga, T; Onaka, H; Shinzato, N; Kakeya, H

  JOURNAL OF ANTIBIOTICS     2026.03 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Microbial distribution on sandstone with honeycomb weathering along rocky coasts in a subtropical environment

  Takayuki Ogata, Naoya Shinzato, Hitoshi Matsubara

  Terrestrial, Atmospheric and Oceanic Sciences ( Springer Nature )  37 ( 9 )   2026.02 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• A new pyranonaphthoquinone, actinoquinonal A, and its congeners from the combined-culture of <i>Streptomyces</i> sp. 23-50 and <i>Tsukamurella pulmonis </i>TP-B0596

  Yanagisawa, K; Kaneko, K; Ikeda, H; Iwata, S; Muranaka, A; Koshino, H; Nagao, N; Watari, S; Nishimura, S; Shinzato, N; Onaka, H; Kakeya, H

  JOURNAL OF ANTIBIOTICS ( Journal of Antibiotics )  78 ( 6 ) 350 - 358   2025.05 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

  　View Summary

  The combined-culture of actinomycetes with mycolic acid-containing bacteria (MACB) Tsukamurella pulmonis TP-B0596 is a promising strategy to produce cryptic metabolites in actinomycetes. In this study, Streptomyces sp. 23-50 was identified as an appropriate strain for co-culturing with T. pulmonis TP-B0596 using on-gel combined-culture screening of 160 strains of actinomycetes. A new pyranonaphthoquinone, actinoquinonal A (1), along with two known congeners, compound 2 and mevashuntin (3), were isolated from the combined-culture of Streptomyces sp. 23-50 with T. pulmonis TP-B0596 based on global natural product social (GNPS) molecular networking. The planar structures of 1-3 were elucidated by analyzing 2D nuclear magnetic resonance (NMR) and LC-MS/MS spectral data, and the absolute configurations of 1 and 3 were unambiguously determined by comparing experimental and calculated ECD spectra. Moreover, the combined-culture characteristic metabolites, including 3, were enhanced when Streptomyces sp. 23-50 was cultured in the presence of pravastatin, an inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase in the mevalonate pathway, suggesting that T. pulmonis TP-B0596 triggered a shunt in the mevalonate pathway of Streptomyces sp. 23-50. Notably, compounds 1 and 3 exhibited cytotoxicity against human cervical epithelioid carcinoma HeLa S3 (IC50 = 60.5 μM for 1, 0.67 μM for 3) and human colorectal cancer HT29 cells (IC50 = 101.9 μM for 1, 0.45 μM for 3).

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• New antimalarial iromycin analogs produced by <i>Streptomyces</i> sp. RBL-0292

  Kimura, SI; Watanabe, Y; Shibasaki, S; Shinzato, N; Inahashi, Y; Sunazuka, T; Hokari, R; Ishiyama, A; Iwatsuki, M

  JOURNAL OF ANTIBIOTICS ( Journal of Antibiotics )  77 ( 5 ) 272 - 277   2024.03 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

  　View Summary

  Two new antimalarial compounds, named prenylpyridones A (1) and B (2), were discovered from the actinomycete cultured material of Streptomyces sp. RBL-0292 isolated from the soil on Hamahiga Island in Okinawa prefecture. The structures of 1 and 2 were elucidated as new iromycin analogs having α-pyridone ring by MS and NMR analyses. Compounds 1 and 2 showed moderate in vitro antimalarial activity against chloroquine-sensitive and chloroquine-resistant Plasmodium falciparum strains, with IC50 values ranging from 80.7 to 106.7 µM.

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• Precezomycin, a novel antibiotic biosynthetic precursor of cezomycin, from actinomycete <i>Kitasatospora putterlickiae</i> 10-13

  Mao, D; Yu, PW; Shinzato, N; Zhang, L; Zheng, WP; Lu, S; Kakeya, H

  JOURNAL OF ANTIBIOTICS ( Journal of Antibiotics )  77 ( 3 ) 182 - 184   2024.03 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

  　View Summary

  A novel antibiotic biosynthetic precursor of cezomycin, named precezomycin (1), was isolated from culture broth of actinomycete Kitasatospora putterlickiae 10-13. The planar structure was determined by 1D/2D NMR and HR(ESI)MS data analyses, and the absolute configurations were established by TDDFT calculation of ECD spectra. Precezomycin (1) exhibited moderate antibacterial activity against gram-positive bacteria including Staphylococcus aureus and Bacillus subtilis. The discovery of 1 extends the natural product family of cezomycin and provides a new insight into understanding the biosynthetic process of these polyether ionophore metabolites.

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Books 【 display / non-display 】

Books 【 display / non-display 】

• Microbiology Monographs 19, (Endo)symbiotic methangenic archaea 2nd edition

  Shinzato N, Takeshita K, Kamagata Y ( Part: Allotment Writing ,  Methanogenic and bacterial endosymbionts of anaerobic free-living ciliates )

  Springer-Verlag, Berlin Heidelberg  2018.11

Other Papers 【 display / non-display 】

Other Papers 【 display / non-display 】

• Functional genomics of the Lactobacillus paracasei strain LC-Ikematsu isolated from the Okinawan natural environment

  Tada I, Saitoh S, Aoyama H, Shinzato N, Morita K, Yamamoto N, Ikematsu S

  Bulletin of National Institute of Technology, Okinawa College   11   1 - 8   2017.03

   

• ヒョウタンナガカメムシ類で見つかった環境獲得型細胞内共生

  竹下和貴, 松浦優, 孟憲英, 孟憲英, 三谷恭雄, 新里尚也, 菊池義智, 菊池義智

  日本応用動物昆虫学会大会講演要旨   61st   92   2017.03

   

  J-GLOBAL

• カメムシ類における垂直伝播を伴わない細胞内共生

  菊池義智, 竹下和貴, 竹下和貴, 松浦優, 孟憲英, 新里尚也, 三谷恭雄

  日本微生物生態学会大会(Web)   2017   ROMBUNNO.O‐033 (WEB ONLY)   2017

   

  J-GLOBAL

Presentations 【 display / non-display 】

Presentations 【 display / non-display 】

• Discovery of Sulfur-Containing Cyclic lipopeptides Thioamycolamides A-E, Produced by the Rare Actinomycete <i>Amycolatopsis</i> sp.

  Pan C., Kuranaga T., Liu C., Lu S., Shinzato N., Kakeya H.

  Symposium on the Chemistry of Natural Products, symposium papers  2020  -  2020 

  CiNii Research

• Comparative analysis between bioactive compound and bacterial symbiont in marine sponges

  Tanaka S, Shirai Y, Ito M, Shinzato N

  Japan Symbiosis Society  2018.11  -  2018.11 

• 嫌気性繊毛虫におけるメタン菌、バクテリアとの共生関係

  竹下和貴, 山田尊貴, 川原邑斗, 成廣隆, 鎌形洋一, 新里尚也

  日本微生物生態学会  (沖縄コンベンションセンター, 宜野湾)  2018.07  -  2018.07 

• 沖縄産Axinella 属カイメン類の共生微生物相 - 地球の反対側との比較

  砂川春樹, Sanghwa Park, 青山洋昭, 長濱秀樹, 金本明彦, 齋藤星耕, 伊藤通浩, 新里尚也

  日本微生物生態学会  (沖縄コンベンションセンター, 宜野湾)  2018.07  -  2018.07 

• 沖縄微生物ライブラリーを利用した植物病原糸状菌の抑制

  上野誠, 田村朋子, Yokoyama Y, Ganphung R, 新里尚也, 伊藤通浩

  日本微生物生態学会  (沖縄コンベンションセンター, 宜野湾)  2018.07  -  2018.07 

Grant-in-Aid for Scientific Research 【 display / non-display 】

Grant-in-Aid for Scientific Research 【 display / non-display 】

• Grant-in-Aid for Scientific Research(B)

  Project Year: 2021.04  -  2026.03 

  Direct: 12,900,000 (YEN)  Overheads: 16,770,000 (YEN)  Total: 3,870,000 (YEN)

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2021.04  -  2024.03 

  Direct: 3,300,000 (YEN)  Overheads: 4,290,000 (YEN)  Total: 990,000 (YEN)

• Elucidating the molecular basis of symbiotic association between anaerobic ciliates and their endosymbionts

  Grant-in-Aid for Research Activity start-up

  Project Year: 2017.08  -  2019.03 

  Investigator(s): TAKESHITA Kazutaka, SHINZATO Naoya 

  Direct: 2,100,000 (YEN)  Overheads: 2,730,000 (YEN)  Total: 630,000 (YEN)

  　View Summary

  Some free-living anaerobic ciliates establish tripartite symbiotic relationships with methanogenic archaea and bacteria with unknown functions. To elucidate the molecular basis of these symbioses, I tried to apply “feeding RNAi experiment" established in aerobic ciliates, Paramecium spp., to a cultured anaerobic ciliate of the genus Trimyema, which has been stably maintained for over 20 years in the laboratory. The application has not yet succeeded but I continue to try to apply it. On the other hand, an anaerobic ciliate, strain GW7, has been newly isolated successfully. I taxonomically identified its methanogenic and bacterial endosymbionts based on their 16S rRNA sequences and revealed that the bacterial symbiont is phylogenetically related to the intranuclear symbionts of aerobic ciliates.

• Grant-in-Aid for Scientific Research(B)

  Project Year: 2017.04  -  2021.03 

  Direct: 13,500,000 (YEN)  Overheads: 4,050,000 (YEN)  Total: 17,550,000 (YEN)

• Establishing reintroduction and phylogenetic conservation measures based on the genetic diversity evaluation of the endangered aquatic insect

  Grant-in-Aid for Scientific Research(C)

  Project Year: 2015.04  -  2019.03 

  Investigator(s): KURANISHI Ryoichi, NISHIHARA Syogo, SHINZATO Naoya, HORIGUCHI Tomohiro, ONO Tomoki, SADO Tetsuya 

  Direct: 3,900,000 (YEN)  Overheads: 5,070,000 (YEN)  Total: 1,170,000 (YEN)

  　View Summary

  For the conservation of an endangered insect species, we examined the method of phylogenetic preservation and reintroduction based on genetic information. For obtaining genetic information, we developed microsatellite markers using giga sequencer platforms. We examined the genetic diversity, continuity, and uniqueness of regional populations using these microsatellite markers. On the basis of these results, a plan was implemented to reintroduce phylogenetically conserved populations in the field.

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Social Activity 【 display / non-display 】

Social Activity 【 display / non-display 】

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