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Published Papers 【 display / non-display 】

• Low-Intensity Extracorporeal Shock Wave Therapy Restores Passive Urethral Closure Mechanism in Vaginal Distension Rat Model

  Akimoto, T; Ashikari, A; Kurobe, M; Takamatsu, G; Akiyama, Y; Kume, H; Miyazato, M

  INTERNATIONAL UROGYNECOLOGY JOURNAL ( International Urogynecology Journal )    2026.05 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Low-intensity energy shock wave therapy modulates bladder function and anxiety-like behavior in maternal separation rats.

  Kamijo TC, Kurobe M, Akimoto T, Miyazato M

  International urology and nephrology ( International Urology and Nephrology )  58 ( 4 ) 1307 - 1320   2026.04 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  AIMS: To investigate whether low-intensity energy shock wave therapy (LiESWT) applied to the bladder can alleviate maternal separation (MS)-induced lower urinary tract dysfunction and anxiety-like behavior in a rat model. METHODS: Sprague-Dawley rat pups were divided into normal, MS, and MS + LiESWT groups. MS was performed on postnatal days 2-14. At 6 weeks of age, the MS + LiESWT group received shock wave therapy (0.12 mJ/mm2, 2 Hz, 200 shocks per session, nine sessions) in the bladder region. At 9 weeks of age, all groups underwent anxiety-like behavior assessment using the elevated plus maze test, followed by metabolic cage evaluation, cystometry, and histology to assess bladder function and morphology. RESULTS: Compared to normal rats, MS rats exhibited increased bladder weight, shortened intercontraction intervals, and increased anxiety-like behavior. LiESWT treatment normalized bladder weight and improved urinary frequency compared to MS rats, and reduced anxiety-like behavior, as evidenced by recovery of time spent in the open arms of the elevated plus maze. Sex-related differences were observed, with effects being more pronounced in females. This was particularly evident in the voiding frequency during the dark phase and the adrenal gland weight. CONCLUSIONS: LiESWT applied to the bladder improved bladder dysfunction and reduced anxiety-like behavior in MS rats, suggesting potential interactions between bladder function and emotional state that may involve shared neural circuits. The findings indicated the potential therapeutic applications of LiESWT for improving the stress-induced dysfunction affecting the integrated neurobiological pathways that govern both bladder control and behavioral regulation.

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• Characterization of optogenetically activated inhibitory inputs onto cholinergic motor neurons in the spinal dorsolateral nucleus

  Kamijo, TC; Karnup, S; Matsuoka, K; Kimura, S; Miyazato, M; Daugherty, SL; Beckel, JM; Yoshimura, N

  PHYSIOLOGICAL REPORTS ( Physiological Reports )  13 ( 24 ) e70703   2025.12 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  Inhibitory control of external urethral sphincter motor neurons (EUS-MNs) in the spinal dorsolateral nucleus (DLN), which corresponds to a portion of Onuf's nucleus in humans, is essential for normal micturition by inducing EUS relaxation during voiding; yet synaptic mechanisms remain poorly characterized. Using neonatal mice P8-P12, we developed a slicing technique-cutting spinal cords at 150° from the coronal plane (30° from the horizontal plane in the agarose block), for maximizing EUS-MNs captured per slice. Using transgenic mice co-expressing channelrhodopsin-2 in inhibitory interneurons (VGAT-ChR2) and GFP in cholinergic neurons (ChAT-GFP), we investigated inhibitory synaptic transmission onto EUS-MNs. Optogenetic activation evoked robust inhibitory postsynaptic potentials (IPSPs), classified as sustained or transient based on temporal profiles. Pharmacology revealed that sustained IPSPs contained both glycinergic and GABAergic components, while GABAA receptors predominantly mediated transient IPSPs. Strychnine (1 μM) selectively blocked glycinergic transmission, while bicuculline (10 μM) eliminated GABAergic components. Insensitivity to glutamatergic antagonists (CNQX and AP5) confirmed purely inhibitory responses. Our findings demonstrate segregation of inhibitory inputs onto EUS-MNs, with glycinergic and GABAergic transmission contributing to sustained and transient inhibition, respectively, establishing the methodological foundation for investigating inhibitory circuit dynamics in pathological conditions such as spinal cord injury with deficient inhibitory control.

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• Time-dependent bladder activity changes in streptozotocin-induced female diabetic rats

  Izumi, K; Kamijo, TC; Oshiro, T; Kimura, R; Ashikari, A; Kurobe, M; Akimoto, T; Miyazato, M

  PHYSIOLOGICAL REPORTS ( Physiological Reports )  13 ( 4 ) e70220   2025.02 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  This study aimed to investigate the long-term physiological and morphological changes in the bladders of diabetic rats. Sixty-nine female Sprague-Dawley rats were divided into a control and six diabetic (3 days and 2, 4, 8, 12, and 24 weeks after induction of type 1 diabetes) groups. Metabolic cages and cystometry were used to evaluate bladder function. Bladder contractility was assessed using an organ bath test, and Masson's trichrome staining was performed. In the metabolic cage study, the urination frequency during the dark period significantly increased in the early stages at 3 days of diabetes (p < 0.05). The voiding interval significantly increased (p < 0.05) at 8-12 weeks of diabetes, while the residual urine volume and voiding efficiency worsened at 24 weeks. In the organ bath study, the dose-response curve of carbachol for median effective concentration did not change; however, the bladder contractile force was enhanced at 8 weeks (p = 0.028). Histological analysis revealed increased fibrosis at 4 weeks of diabetes. Diabetic bladder dysfunction is characterized by storage and voiding bladder activity changes in the early stages that induces urinary frequency and reduced voiding efficiency in the late phase; this turning point occurs at 8 weeks after diabetes.

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• Genome-wide association studies for pelvic organ prolapse in the Japanese population

  Matsunami, M; Imamura, M; Ashikari, A; Liu, XX; Tomizuka, K; Hikino, K; Miwa, K; Kadekawa, K; Suda, T; Matsuda, K; Miyazato, M; Terao, C; Maeda, S

  COMMUNICATIONS BIOLOGY ( Nature Research )  7 ( 1 ) 1188 - 9   2024.09

  Type of publication: Research paper (scientific journal)

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  Pelvic organ prolapse (POP) affects approximately 40% of elderly women, characterized by thedescent of the pelvic organs into the vaginal cavity. Here we present the results of a genome-wideassociation study (GWAS) for susceptibility to POP comprising 771 cases and 76,625 controls in theJapanese population. We identified a significant association of WT1 locus with POP in the Japanesepopulation; rs10742277; odds ratio (OR) = 1.48, 95% confidence interval (CI), 1.29–1.68,P = 6.72 × 10−9. Subsequent cross-ancestry GWAS meta-analysis combining the Japanese data andpreviously reported European data, including 28,857 cases and 622,916 controls, identified FGFR2locus as a novel susceptibility locus to POP (rs7072877; OR = 1.06, 95% CI, 1.04–1.08,P = 4.11 × 10−8). We also observed consistent directions of the effects for 21 out of 24 European GWASderived loci (binomial test P = 2.8 × 10−4), indicating that most of susceptibility loci for POP are sharedacross the Japanese and European populations.

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Other Papers 【 display / non-display 】

Other Papers 【 display / non-display 】

• 前立腺癌に対するホルモン療法は腎機能障害を引き起こすか?

  三塚 浩二, 喜屋武 淳, 佐藤 友紀, 折笠 一彦, 青木 大志, 宮里 実, 荒井 陽一

  日本泌尿器科学会総会 ( (一社)日本泌尿器科学会総会事務局 )  104回   PP1 - 088   2016.04

   

• PDE5阻害剤タダラフィルのラット尿禁制反射への作用

  泉 秀明, 海法 康裕, 川守田 直樹, 宮里 実, 中川 晴夫, 荒井 陽一

  日本泌尿器科学会総会 ( (一社)日本泌尿器科学会総会事務局 )  104回   PP3 - 114   2016.04

   

• 年齢が前立腺癌ホルモン療法による代謝の変化に与える影響についての検討

  三塚 浩二, 喜屋武 淳, 佐藤 友紀, 折笠 一彦, 宮里 実, 青木 大志, 栫井 成彦, 福士 泰夫, 浪間 孝重, 荒井 陽一

  日本老年泌尿器科学会誌 ( 日本老年泌尿器科学会 )  28   47 - 47   2015.12

   

• 前立腺癌内分泌療法に伴うsarcopenic obesityの検討 CTによる脂肪・筋肉量測定に基づく解析

  三塚 浩二, 喜屋武 淳, 佐藤 友紀, 折笠 一彦, 浪間 孝重, 宮里 実, 荒井 陽一

  日本泌尿器科学会総会 ( (一社)日本泌尿器科学会総会事務局 )  103回   501 - 501   2015.04

   

Grant-in-Aid for Scientific Research 【 display / non-display 】

Grant-in-Aid for Scientific Research 【 display / non-display 】

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2025.04  -  2028.03 

  Direct: 3,500,000 (YEN)  Overheads: 4,550,000 (YEN)  Total: 1,050,000 (YEN)

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2025.04  -  2028.03 

  Direct: 3,600,000 (YEN)  Overheads: 4,680,000 (YEN)  Total: 1,080,000 (YEN)

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2025.04  -  2028.03 

  Direct: 3,600,000 (YEN)  Overheads: 4,680,000 (YEN)  Total: 1,080,000 (YEN)

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2025.04  -  2028.03 

  Direct: 3,500,000 (YEN)  Overheads: 4,550,000 (YEN)  Total: 1,050,000 (YEN)

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2025.04  -  2028.03 

  Direct: 3,500,000 (YEN)  Overheads: 4,550,000 (YEN)  Total: 1,050,000 (YEN)

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Social Activity 【 display / non-display 】

Social Activity 【 display / non-display 】

• Department of Systems Physiology 

  2025.12