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Published Papers 【 display / non-display 】

• Vegetation variety affected by local environments in a coral reef lagoon

  Tozaki, K; Nishihara, GN; Kawate, A; Konishi, T; Sato, Y; Ito, M; Fujimura, H; Tanaka, A

  PHYCOLOGICAL RESEARCH ( Phycological Research )    2024.01 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• The Cell Wall Characterization of Brown Alga <i>Cladosiphon okamuranus</i> during Growth

  Miwa, Y; Awanthi, MGG; Soga, K; Tanaka, A; Ito, M; Numata, Y; Sato, Y; Konishi, T

  PLANTS-BASEL ( Plants )  12 ( 18 )   2023.09 [ Peer Review Accepted ]

  Type of publication: Research paper (other science council materials etc.)

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• Characterization of cell wall polysaccharide from <i>Cladosiphon okamuranus</i> cultivated in different locations

  Mahanama Geegana Gamage Awanthi, Natsuki Nakasone, Hirosuke Oku, Kanefumi Kitahara, Michihiro Ito, Atsuko Tanaka, Yoichi Sato, Yuichiro Numata, Teruko Konishi

  Phycological Research ( Wiley )  72 ( 1 ) 3 - 11   2023.08 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  SUMMARY Cladosiphon okamuranus is an excellent source of fucoidan among any brown algae spp. However, the structure of polysaccharide varies significantly depending on the species, harvesting season and geographical location. To understand how geographic location affects the structure of cell wall polysaccharide, C. okamuranus cultivated in eight places (Bise, Katsuren, Yonashiro, Shikiya, Karimata, Nishihara, Hisamatsu and Ishigaki) in Ryukyu archipelago were collected during the peak harvesting time and the cell wall polysaccharide was analyzed physiochemically. The cell wall was sequentially fractionated into five fractions and its 80% was consisted of hot water fraction (HW) (60–70%) and hemicellulose‐I fraction (HC‐I) (15–20%), which mainly contained fucoidan composed of fucose (Fuc), glucuronic acid (GlcA) and sulfate (SO₃⁻). Although the chemical composition and molecular weight of fucoidan in HW was slightly different at different places, the composition of Fuc, GlcA and SO₃⁻ was constant in the molar ratio of 1.0:0.5:0.7–1.0 respectively, suggesting that relatively similar structural fucoidan from C. okamuranus can be obtained from any geographical location in Okinawa Prefecture during peak time. However, considerable variation in the sugar composition in HC‐I was observed, despite the uniform molecular weight and SO₃⁻. Particularly, HC‐I from Bise, where is the northern part of Okinawa Island, is composed of significantly higher amounts of Glc, Gal, Man, Xyl and morphologically thicker lateral branches compared to algae from the other parts of the island.

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• Fractionation and characterization of cell wall polysaccharides from the brown alga Cladosiphon okamuranus

  Awanthi Mahanama, Geegana Gamage, Manatsu Umosa, Yoshiaki Yuguchi, Hirosuke Oku, Kanefumi Kitahara, Michihiro Ito, Atsuko Tanaka, Teruko Konishi

  Carbohydrate Research (in press) ( Carbohydrate Research )  523   108722 - 108722   2023.01 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  Brown algae contain a polysaccharide-rich cell wall, mainly composed of alginate and fucoidan which have been extensively studied for their individual structure and bioactivities. Particularly, the cell wall of Cladosiphon okamuranus is rich in fucoidan rather than alginate. However, little is known about its arrangement or interlinking with other polysaccharides such as cellulose in the cell wall. To determine its structure in detail, the cell wall was sequentially fractionated into five fractions: hot water (HW), ammonium oxalate, hemicellulose-I (HC-I), HC-II, and cellulose. Almost 80% of the total cell wall recovered from alcohol insoluble residue in C. okamuranus consisted of HW and HC-I, which mainly contained fucoidan composed of fucose, glucuronic acid, and sulfate in molar ratios of 1.0:0.3:0.9 and 1.0:0.2:0.3, respectively. Methylation analysis revealed that fucoidan in HW and HC-I structurally differed in terms of content of sulfate, and sugar residue which was 1,4-linked xylose and 1,4-linked fucose. Small angle X-ray scattering measurements also showed distinct conformational differences between HW and HC-I. These structural heterogeneities of fucoidan may be related to their localization, and fucoidan in HC-I may be involved in reinforcing cell wall structure by cross-linking to cellulose.

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• Targeted single-cell genomics reveals novel host adaptation strategies of the symbiotic bacteria Endozoicomonas in Acropora tenuis coral

  Ide K, Nishikawa Y, Maruyama T, Tsukada Y, Kogawa M, Takeda H, Ito H, Wagatsuma R, Miyaoka R, Nakano Y, Kinjo K, Ito M, Hosokawa M, Yura K, Suda S, Takeyama H

  Microbiome ( Microbiome )  10 ( 1 ) 220   2022.12 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  Abstract Background Endozoicomonas bacteria symbiosis with various marine organisms is hypothesized as a potential indicator of health in corals. Although many amplicon analyses using 16S rRNA gene have suggested the diversity of Endozoicomonas species, genome analysis has been limited due to contamination of host-derived sequences and difficulties in culture and metagenomic analysis. Therefore, the evolutionary and functional potential of individual Endozoicomonas species symbiotic with the same coral species remains unresolved. Results In this study, we applied a novel single-cell genomics technique using droplet microfluidics to obtain single-cell amplified genomes (SAGs) for uncultured coral-associated Endozoicomonas spp. We obtained seven novel Endozoicomonas genomes and quantitative bacterial composition from Acropora tenuis corals at four sites in Japan. Our quantitative 16S rRNA gene and comparative genomic analysis revealed that these Endozoicomonas spp. belong to different lineages (Clade A and Clade B), with widely varying abundance among individual corals. Furthermore, each Endozoicomonas species possessed various eukaryotic-like genes in clade-specific genes. It was suggested that these eukaryotic-like genes might have a potential ability of different functions in each clade, such as infection of the host coral or suppression of host immune pathways. These Endozoicomonas species may have adopted different host adaptation strategies despite living symbiotically on the same coral. Conclusions This study suggests that coral-associated Endozoicomonas spp. on the same species of coral have different evolutional strategies and functional potentials in each species and emphasizes the need to analyze the genome of each uncultured strain in future coral-Endozoicomonas relationships studies.

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Other Papers 【 display / non-display 】

Other Papers 【 display / non-display 】

• Annual dynamics of microflora associated with a reef coral Acropora tenuis in Okinawa s hallow reef(The Joint Meeting of Japanese Environmental Microbiology-related Associations 2014)

  ( the Japanese Society of Soil Microbiology )  68 ( 2 ) 97 - 97   2014.10

   

• 1P-162 Bioenrichment of bacteria degrading seaweed-derived polysaccharides in the gut of marine invertebrates

  Ito Michihiro, Watanabe Kotaro, Mori Tetsushi, Maruyama Toru, Midorikawa Naoko, Cho Seinen, Takeyama Haruko

    65   58 - 58   2013

   

• PK-25 小麦穂由来の新規細菌による小麦穀粒中のかび毒の分解(PK 相互作用・共生,ポスター発表)

  伊藤 通浩, 佐藤 育男, 小板橋 基夫, 吉田 重信, 今井 真千子, 對馬 誠也

  日本微生物生態学会講演要旨集 ( 日本微生物生態学会 )  ( 28 ) 176 - 176   2012.09

   

• 2C-3 かび毒デオキシニバレノールを代謝する細菌由来P450システムの固定と機能解析(G.生理/代謝/増殖,口頭発表)

  伊藤 通浩, 石坂 眞澄, 吉田 慎一朗, 生長 陽子, 佐藤 育男, 小板橋 基夫, 對馬 誠也

  日本微生物生態学会講演要旨集 ( 日本微生物生態学会 )  ( 26 ) 90 - 90   2010.11

   

• P-111 グラム陰性細菌SS5株からのデオキシニバレノール脱水素酵素の精製と諸性質(G.生理/代謝/増殖,ポスター発表)

  佐藤 育男, 伊藤 通浩, 石坂 眞澄, 生長 陽子, 吉田 重信, 小板橋 基夫, 對馬 誠也

  日本微生物生態学会講演要旨集 ( 日本微生物生態学会 )  ( 26 ) 150 - 150   2010.11

   

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Presentations 【 display / non-display 】

Presentations 【 display / non-display 】

• Anaerobic ciliate strains and symbiotic methanogenic archaea established from a sewage treatment center in Okinawa

  Naoya Shinzato, Rintaro Furugen, Toshinori Taki, Hibiki Hashimoto, Michihiro Ito

  2023.11  -  2023.11 

• Identification of antimicrobial substances produced by marine microorganisms from reef-building corals

  Riri KINJO, Takahiro ISHII, Shota MORI, Kazuhiko SAKAI, Naoya SHINZATO, Michihiro ITO

  2023.03  -  2023.03 

• Are reef-building coral microbiota at sites of high temperature stress less likely to change under high temperature? : Verification through aquarium experiments

  Shota MORI, Michihiro ITO, Jun ISHIDA C, Yuji ISE, Cristin Manullang, Ranta KINJO, Toshiko UEZU, Ryosuke KIMURA, Naoya SHINZATO, Kazuhiko SAKAI

  The Japanese Coral Reef Society 25th Annual Conference  2022.11  -  2022.11 

• Specific Detection of Coral-Associated Ruegeria, a Potential Probiotic Bacterium, in Corals and Subtropical Seawater

  2022.05  -  2022.05