Matsushita Masayuki

写真a

Title

Professor

Researcher Number(JSPS Kakenhi)

30273965

Laboratory Address

207 Uehara,Nishihara,Okinawa

Mail Address

E-mail address

Laboratory Phone number

-2314

Laboratory Fax number

-2314
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Current Affiliation Organization 【 display / non-display 】

  • Duty   University of the Ryukyus   Graduate School of Medicine   Professor  

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External Career 【 display / non-display 】

  • 2010.04
    　
    　

    University of the Ryukyus, Graduate School of Medicine, Professor  

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Research Interests 【 display / non-display 】

  • Physiology

  • Neuroscience

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Research Areas 【 display / non-display 】

  • Life Science / Medical biochemistry

  • Life Science / Cell biology

  • Life Science / Molecular biology

  • Life Science / Clinical pharmacy

  • Life Science / Physiology

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Research Theme 【 display / non-display 】

  • Neuroscience

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Published Papers 【 display / non-display 】

  • Induced hepatic stem cells are suitable for human hepatocyte production

    Yoshiki Nakashima, Chika Miyagi-Shiohira,Issei Saitoh, Masami Watanabe, Masayuki Matsushita, Masayoshi Tsukahara, Hirofumi Noguchi

    iScience ( ELSEVER )  25 ( 10 )   2022.09 [ Peer Review Accepted ]

    Type of publication: Research paper (scientific journal)

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    Human hepatocytes were transfected with Sendai virus vectors (SeV) expressing OCT3/4, SOX2, KLF4, and C-MYC to produce hepatocyte-derived induced pluripotent stem cells (iPSCs). The messenger RNA (mRNA) expression of undifferentiated markers (passage 19-21) and hepatocyte-specific markers (HSMs) (passage 0-20) in 48 established hepatocyte-derived iPSC-like colonies was examined. Among the 48 clones, 10 clones continuously expressed HSM mRNA (HNF1β and HNF4α) in passage 0-20. The colonies which expressed HSMs (iTS-L cells: induced tissue-specific stem cells from liver) showed a different tendency in microarray and methylation analyses to fibroblast-derived iPSCs (strain: 201B7). iTS-L cells were less likely to form teratomas in mice than iPSCs (He). The iTS-L cells were differentiated into hepatocyte-like cells more efficiently than iPSCs (He) or iPSCs (201B7). These data suggest that SeV expressing OCT3/4, SOX2, KLF4, and C-MYC induce the generation of iPSCs and iTS-L cells.

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  • Haplotype phasing of a bipolar disorder pedigree revealed rare multiple mutations of SPOCD1 gene in the 1p36-35 susceptibility locus

    Gakuya Takamatsu, Kumiko Yanagi, Kae Koganebuchi, Fuyuko Yoshida, Jun-Seok Lee, Kanako Toyama, Kotaro Hattori, Chiaki Katagiri, Tsuyoshi Kondo, Hiroshi Kunugi , Ryosuke Kimura, Tadashi Kaname, Masayuki Matsushita

    J Affect Disord ( ELSEVER )  310   96 - 105   2022.08 [ Peer Review Accepted ]

    Type of publication: Research paper (scientific journal)

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    Background: The etiology of bipolar disorder (BD) is poorly understood. Considering the complexity of BD, pedigree-based sequencing studies focusing on haplotypes at specific loci may be practical to discover high-impact risk variants. This study comprehensively examined the haplotype sequence at 1p36-35 BD and recurrent depressive disorder (RDD) susceptibility loci. Methods: We surveyed BD families in Okinawa, Japan. We performed linkage analysis and determined the phased sequence of the affected haplotype using whole genome sequencing. We filtered rare missense variants on the haplotype. For validation, we conducted a case-control genetic association study on approximately 3000 Japanese subjects. Results: We identified a three-generation multiplex pedigree with BD and RDD. Strikingly, we identified a significant linkage with mood disorders (logarithm of odds [LOD] = 3.61) at 1p36-35, supported in other ancestry studies. Finally, we determined the entire sequence of the 6.4-Mb haplotype shared by all affected subjects. Moreover, we found a rare triplet of missense variants in the SPOCD1 gene on the haplotype. Notably, despite the rare frequency, one heterozygote with multiple SPOCD1 variants was identified in an independent set of 88 BD type I genotyping samples. Limitations: The 1p36-35 sequence was obtained from only a single pedigree. The replicate sample was small. Short-read sequencing might miss structural variants. A polygenic risk score was not analyzed. Conclusion: The 1p36-35 haplotype sequence may be valuable for future BD variant studies. In particular, SPOCD1 is a promising candidate gene and should be validated.

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  • Metabolically and immunologically beneficial impact of extra virgin olive and flaxseed oils on composition of gut microbiota in mice.

    Millman J, Okamoto S, Kimura A, Uema T, Higa M, Yonamine M, Namba T, Ogata E, Yamazaki S, Shimabukuro M, Tsutsui M, Matsushita M, Ikematsu S, Masuzaki H

    European journal of nutrition ( European Journal of Nutrition )    2019.09 [ Peer Review Accepted ]

    Type of publication: Research paper (scientific journal)

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  • Adipose tissue-derived mesenchymal stem cells ameliorate bone marrow aplasia related with graft-versus-host disease in experimental murine models.

    Nishi Y, Murakami A, Murayama Y, Tsukahara N, Okamoto S, Nakachi S, Morichika K, Tamaki K, Noguchi H, Matsushita M, Karube KN, Fukushima T, Morishima S, Kishimoto H, Masuzaki H

    Transplant immunology ( Transplant Immunology )  55   101205   2019.08 [ Peer Review Accepted ]

    Type of publication: Research paper (scientific journal)

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  • Validation of a novel digital health monitoring system to measure the volume of voided urine.

    Miyazato M, Yonemoto K, Ashikari A, Saito S, Yamashiro K, Uehara M, Masuzaki H, Ishida H, Matsushita M

    Neurourology and urodynamics ( Neurourology and Urodynamics )  38 ( 4 ) 1106 - 1110   2019.04 [ Peer Review Accepted ]

    Type of publication: Research paper (scientific journal)

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