TAKARA Kensaku

写真a

Title

Professor

Researcher Number(JSPS Kakenhi)

00305187

Mail Address

E-mail address

2
To the head of this page.▲

Current Affiliation Organization 【 display / non-display 】

  • Concurrently   University of the Ryukyus   The United Graduate School of Agricultural Sciences, Kagoshima University   Biological Science and Technology   Professor  

  • Concurrently   University of the Ryukyus   Graduate School of Agriculture   Subtropical Agriculture   Professor  

  • Duty   University of the Ryukyus   Faculty of Agriculture   Bioscience and Biotechnology   Professor  

To the head of this page.▲

University 【 display / non-display 】

  • 　
    -
    1993.03

    University of the Ryukyus   Faculty of Agriculture   Graduated

  • 　
    -
    1993.03

    University of the Ryukyus   Faculty of Agriculture   Graduated

  • 　
    -
    1995.03

    University of the Ryukyus   Graduate School, Division of Agriculture   Graduated

To the head of this page.▲

Graduate School 【 display / non-display 】

  • 　
    -
    1995.03

    University of the Ryukyus  Graduate School, Division of Agriculture  Master's Course  Completed

To the head of this page.▲

Academic degree 【 display / non-display 】

  • Kagoshima University -  Doctor of Agriculture

To the head of this page.▲

External Career 【 display / non-display 】

  • 2018.12
    　
    　

    University of the Ryukyus, Faculty of Agriculture, Department of Subtropical Biochemistry and Biotechnology, Food Science and Nutrition, Professor  

  • 2009.04
    -
    2018.11

    University of the Ryukyus, Faculty of Agriculture, Department of Subtropical Biochemistry and Biotechnology, Food Science and Nutrition, Associate Professor  

  • 2007.04
    -
    2009.03

    University of the Ryukyus, Faculty of Agriculture, Bioscience and Biotechnology, Applied Biochemistry, Associate Professor  

  • 1998.05
    -
    2007.03

    University of the Ryukyus, Faculty of Agriculture, Bioscience and Biotechnology, Applied Biochemistry, Research Assistants  

To the head of this page.▲

Affiliated academic organizations 【 display / non-display 】

  • 1995.04
    -
    Now
     

    Japanese Society for Food Science and Technology 

  • 1999.04
    -
    Now
     

    Japan Society for Bioscience, Biotechnokongy, and Agrochemis 

To the head of this page.▲

Research Interests 【 display / non-display 】

  • 食品機能,食品分析,天然物化学,廃棄物利用,食品化学

To the head of this page.▲

Research Areas 【 display / non-display 】

  • Life Science / Food sciences

To the head of this page.▲

Published Papers 【 display / non-display 】

  • Genetic Characteristics and Volatile Organic Compounds of Leaves and Fruit Edible Parts of Citrus depressa Hayata from Different Geographic Origins

    Moena Oe, Yonathan Asikin, Misaki Kashima, Masashi Yamamoto, Fumimasa Mitsube, Shu-Yen Lin, Kensaku Takara, Koji Wada

    Horticulturae ( MDPI AG )  10 ( 9 ) 939 - 939   2024.09 [ Peer Review Accepted ]

    Type of publication: Research paper (scientific journal)

    　View Summary

    Citrus depressa Hayata is a citrus cultivar grown in Japan and Taiwan. To assess the differences in genetic characteristics and volatile organic components (VOCs) in the leaves and edible parts of the fruits of 23 C. depressa accessions from different geographic origins, the tissues were analyzed using cleaved amplified polymorphic sequence markers and gas chromatography-mass spectrometry. A phylogenetic cluster analysis demonstrated that Kagoshima accessions had a close genetic relationship with one another, with Okinawan “Izumi kugani-like” being the most distinct accession. The predominant volatiles in the leaves were γ-terpinene, p-cymene, limonene, and linalool. Multivariate analysis and volcano plots revealed distinct volatiles in the leaves of each cultivation region: piperitone and citronellal (Kagoshima); 5,9,9-trimethyl-spiro[3.5]non-5-en-1-one (Okinawa); and hexanal (Taiwan). Furthermore, the edible parts of Taiwanese fruits contained abundant amounts of monoterpenes, including linalool and 1,8-cineole. In contrast, Kagoshima and Okinawa accessions were rich in aldehydes and esters, respectively. In conclusion, the genetic and volatile profiles of 23 C. depressa accessions of different origins could be distinguished, and multivariate analysis suggested that C. depressa contains diverse VOCs depending on where it is cultivated. These findings demonstrate the exclusivity of C. depressa resources in each region, which could assist farmers and agro-industries in promoting food products derived from C. depressa fruits.

    • Access this article
      DOI

  • Physicochemical, Carotenoid, Metabolite, and Volatile Organic Compound Profiling of Okinawan Shiikuwasha (Citrus depressa Hayata) and Calamansi (C. microcarpa Bunge)

    Moena Oe, Yonathan Asikin, Fumimasa Mitsube, Yasuyo Sekiyama, Kensaku Takara, Koji Wada

    Applied Sciences ( MDPI AG )  14 ( 15 ) 6746 - 6746   2024.08 [ Peer Review Accepted ]

    Type of publication: Research paper (scientific journal)

    　View Summary

    Shiikuwasha (Citrus depressa Hayata) and calamansi (C. microcarpa Bunge) are distinct citrus species with a strong sour taste that grow naturally in Okinawa, Japan. The present study aimed to characterize the physicochemical traits, total carotenoid content, and taste/nutrient-related metabolite and volatile organic compound (VOC) profiles of the mature fruits from three shiikuwasha cultivation lines (“Izumi kugani”, “Izumi kugani-like”, and “Ogimi kugani”) and calamansi. The shiikuwasha lines had higher ratios of soluble solids/titratable acidity but a lower total carotenoid content than calamansi. There were positive statistical correlations between total carotenoids with red, yellow, and orange indices (a*, b*, and a*/b* color spaces, respectively). Principal component analysis (PCA) revealed that shiikuwasha had higher sucrose and choline contents, while calamansi was richer in sourness-related metabolites such as citrate and malate. PCA plots displayed differences in the VOCs between the two citrus cultivars, wherein volatile accumulation was much richer in juices prepared from the whole fruits than in juices from the edible flesh parts. The plots also showed that monoterpenes and sesquiterpenes differentiated the VOC profiles of shiikuwasha and calamansi. This study reveals differences in the flavor components of the two citrus cultivars and highlights the potential uses of each in local and regional agro-business.

    • Access this article
      DOI

  • Verification of In Vitro Anticancer Activity and Bioactive Compounds in Cordyceps Militaris-Infused Sweet Potato Shochu Spirits.

    Sakao K, Sho C, Miyata T, Takara K, Oda R, Hou DX

    Molecules (Basel, Switzerland) ( Molecules (Basel, Switzerland) )  29 ( 9 )   2024.05 [ Peer Review Accepted ]

    Type of publication: Research paper (scientific journal)

    • Access this article
      DOI
    • Search related information
      PubMed

  • Gas chromatography-mass spectrometry-based electronic nose, glycosidically bound volatile, and alcohol-acyltransferase activity profiles of Okinawan pineapple

    Asikin, Y; Kohama, A; Kudaka, T; Maekawa, R; Kobayashi, T; Takeuchi, M; Kamiyoshihara, Y; Takara, K; Wada, K

    INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY ( International Journal of Food Science and Technology )  59 ( 10 ) 7932 - 7941   2024.04 [ Peer Review Accepted ]

    Type of publication: Research paper (scientific journal)

    　View Summary

    Summary A recent increase in the demand for pineapple necessitates the development of new varieties with superior flavour quality. In this study, we characterised the volatile profiles of three new Okinawan pineapple breeding lines using gas chromatography (GC)–mass spectrometry (MS)‐based electronic nose (e‐nose), glycosylated volatiles, and alcohol‐acyltransferase (AAT) activity measurements. The volatile profiles of the fruits were selected using the GC–MS‐e‐nose and visualised using multivariate statistical plots. The glycosidically bound compounds were collected by solid‐phase extraction and hydrolysed by β‐glucosidase. In total, 12 hydroxyl‐group substances, including chavicol (medicinal‐herbal aroma), geraniol (floral‐sweet), and linalool (floral‐citrus), were released from these glycosides. Crude enzymes containing AAT were extracted, and their in vitro activities in 2‐methylbutyl acetate production were highly related to their contents. These results provide information regarding volatile profiles and bounded‐aroma resources, as well as the esterification potential of new Okinawan pineapple breeding lines for use in the agro‐food industry.

    • Access this article
      DOI

  • In silico and in vitro Characterizations of Rodent Tuber (Typhonium flagelliforme) Mutant Plant Isolates against FXR Receptor on MCF-7 Cells.

    Sianipar NF, Hadisaputri YE, Assidqi K, Salam S, Yusuf M, Destiarani W, Purnamaningsih R, So IG, Takara K, Asikin Y

    Journal of oleo science ( 公益社団法人 日本油化学会 )  73 ( 10 ) 1349 - 1360   2024 [ Peer Review Accepted ]

    Type of publication: Research paper (scientific journal)

    　View Summary

    <p><i>Typhonium flagelliforme</i> (<i>T. flagelliforme</i>) is an Indonesian rodent tuber plant traditionally used to treat cancer diseases. Although gamma-ray irradiation has been used to increase the content in the chemical compounds of the <i>T. flagelliforme</i> plants with anticancer activity ten times effective, the specific effect of the isolated compounds from the mutant plants has never been reported yet. The potential cytotoxic agents were characterized via nuclear magnetic resonance spectroscopy, infrared spectroscopy, and mass spectrometry as stigmasterol and 7α-hydroxyl stigmasterol; and their anticancer activity was investigated. The <i>in silico</i> biochemical profile of the two compounds were analyzed by molecular docking and molecular dynamics simulation to confirm its interaction with the agonist binding site of Farsenoid X receptor (FXR). Stigmasterol and 7α-hydroxyl stigmasterol can act as a competitive regulator with a high-affinity for the FXR. The results also showed that stigmasterol and 7α-hydroxyl stigmasterol were the most potential and active fraction of the <i>T. flagelliforme</i> mutant plant against the MCF-7 human breast cancer cell line, with IC <sub>50</sub> value 9.13 µM and 12.97 µM, compared with cisplastin as a control about 13.20 µM. These results demonstrate the potential of stigmasterol and 7α-hydroxyl stigmasterol in <i>T. flagelliforme</i> mutant plants to act towards cancer diseases.</p>

display all >>

To the head of this page.▲

Books 【 display / non-display 】

  • A Guidebook for Sugarcane in Japan

    K. Takara, D. Matusi, K. Wada ( Part: Multiple Authorship )

    その他の出版機関  2005.03

To the head of this page.▲

Other Papers 【 display / non-display 】

To the head of this page.▲

Presentations 【 display / non-display 】

  • Profiling of carotenoids, metabolites, and volatile aroma compounds in Shiikuwasha (Citrus depressa) and Calamansi (C. microcarpa)

    Yonatahn Asikin, 大江萌那, 光部史将, 関山恭代, 高良健作, 和田浩二

    日本食品科学工学会第70回記念大会  (京都女子大学)  2023.08  -  2023.08 

  • Evaluation of free and glycosidically bound aroma compounds in Okinawan pineapple (Ananas comosus)

    Yonathan Asikin, Kazuki Shimoda, Makoto Takeuchi, Yuta Omine, Ryota Maekawa, Kensaku Takara, Koji Wada

    Food Innovation Asia Conference 2021 - Virtual Conference  2021.06  -  2021.06 

  • へちまのGABA含量と部位，受粉後日数，加熱条件の関係

    前田剛希，花ヶ崎敬資，広瀬直人，恩田聡，棚原尚哉，玉城盛俊，高良健作，和田浩二

    日本食品保蔵科学会第68回大会  (中村学園大学)  2019.06  -  2019.06 

  • Isolation and identification of antifungal compounds from turmeric (Curcuma spp.) and their activities on Fusarium solani sensu lato

    Jesmin Akter, Amzad Hossain, Ayako Sano, Kensaku Takara

    Proceedings of 3rd Global Food Security, Food Safety & Sustainability Conference  (New York, USA)  2018.05  -  2018.05 

  • Isolation and identification of antifungal compounds from turmeric (Curcuma spp.) and their activities on Fusarium solani sensu lato

    Jesmin Akter, Amzad Hossain, Ayako Sano, Kensaku Takara

    Proceedings of 3rd Global Food Security, Food Safety & Sustainability Conference  (New York, USA)  2018.05  -  2018.05 

display all >>

To the head of this page.▲

Industrial Property 【 display / non-display 】

  • no_title

    Industrial Property No no_data  (1900.01.01)

    Unexpectedly No no_data  (1900.01.01)

To the head of this page.▲

Social Activity 【 display / non-display 】

display all >>

To the head of this page.▲

Media Coverage 【 display / non-display 】

  • 沖縄県食品の安全安心講習会

    沖縄県保健医療部衛生薬務課  沖縄県立博物館・美術館　博物館講座室  2019.8

  • 沖縄県食品の安全安心講習会

    沖縄県保健医療部衛生薬務課  沖縄県立博物館・美術館 博物館講座室  2019.8

To the head of this page.▲