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Research Theme 【 display / non-display 】

• Mechanisms regulating trophoblast differentiation

Published Papers 【 display / non-display 】

Published Papers 【 display / non-display 】

• Antifreeze protein type III addition to freezing extender comprehensively improves post-thaw sperm properties in Okinawan native Agu pig.

  Masuda Y, Kheawkanha T, Nagahama A, Kawasaki K, Konno T, Yamanaka K, Tatemoto H

  Animal reproduction science ( Animal Reproduction Science )  252   107232   2023.05 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Addition of l-carnitine to the freezing extender improves post-thaw sperm quality of Okinawan native Agu pig.

  Tatemoto H, Osokoshi N, Hirai M, Masuda Y, Konno T, Yamanaka K

  Theriogenology ( Theriogenology )  188   170 - 176   2022.08 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Endoplasmic reticulum stress attenuation promotes bovine oocyte maturation in vitro.

  Khatun H, Wada Y, Konno T, Tatemoto H, Yamanaka KI

  Reproduction (Cambridge, England) ( Reproduction (Cambridge, England) )  159 ( 4 ) 361 - 370   2020.04 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Role of endoplasmic reticulum stress on developmental competency and cryo-tolerance in bovine embryos.

  Khatun H, Ihara Y, Takakura K, Egashira J, Wada Y, Konno T, Tatemoto H, Yamanaka KI

  Theriogenology ( Theriogenology )  142   131 - 137   2020.01 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Efficient in vitro embryo production using in vivo-matured oocytes from superstimulated Japanese Black cows.

  Egashira J, Ihara Y, Khatun H, Wada Y, Konno T, Tatemoto H, Yamanaka KI

  The Journal of reproduction and development ( 日本繁殖生物学会 )    2019.02

  Type of publication: Research paper (scientific journal)

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  <p>We examined whether the use of <i>in vivo</i>-matured oocytes, collected by ovum pick-up (OPU) from superstimulated Japanese Black cows, can improve the productivity and quality of i<i>n vitro</i> produced embryos. The cows were superstimulated by treatment with progesterone, GnRH, FSH and prostaglandin F2α according to a standardized protocol. The resulting <i>in vivo</i>-matured oocytes were collected by OPU and used subsequently for the other experiments. The immature oocytes from cows in the non-stimulated group were collected by OPU and then subjected to maturation <i>in vitro</i>. We found that the rate of normally distributed cortical granules of the matured oocyte cytoplasm in the superstimulated group was significantly higher than that in the non-stimulated group. The normal cleavage rate (i.e., production of embryos with two equal blastomeres without fragmentation) and freezable blastocyst rate were significantly higher in the superstimulated group than in the non-stimulated group. Among the transferable blastocysts, the ratio of embryos from normal cleavage was also significantly higher in the superstimulated group than in the non-stimulated group. For <i>in vivo</i>-matured oocytes, it was observed that the pregnancy rates were significantly higher when normally cleaved embryos were used for transfer. Taken together, these results suggest that high-quality embryos with respect to developmental kinetics can be efficiently produced with the use of <i>in vivo</i>-matured oocytes collected by OPU from superstimulated Japanese Black cows.</p>

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Presentations 【 display / non-display 】

Presentations 【 display / non-display 】

• Ovarian estrogen negatively regulates uterine eosinophil distribution during implantation window in mice.

  Tomomi Kurane, Akira Morooka, Toshihiro Konno

  OIST and Univ. Ryukyus Joint Symposium  (Okinawa)  2017.10  -  2017.10 

• Mural trophectoderm specific activation of adhesion competence in peri-implantation murine blastocyst

  Ichiko Nishi, Fumie Kawase, Tomomi Murane, Keiichiro Ohta, Hideki Tatemoto, Toshihiro Konno

  4th World Congress of Reproductive Biology  (Okinawa)  2017.09  -  2017.09 

Grant-in-Aid for Scientific Research 【 display / non-display 】

Grant-in-Aid for Scientific Research 【 display / non-display 】

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2015  -  2017 

  Direct: 3,800,000 (YEN)  Overheads: 1,140,000 (YEN)  Total: 4,940,000 (YEN)