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• Mechanisms regulating trophoblast differentiation

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• Endoplasmic reticulum stress attenuation promotes bovine oocyte maturation in vitro.

  Khatun H, Wada Y, Konno T, Tatemoto H, Yamanaka KI

  Reproduction (Cambridge, England) ( Reproduction (Cambridge, England) )  159 ( 4 ) 361 - 370   2020.04 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Role of endoplasmic reticulum stress on developmental competency and cryo-tolerance in bovine embryos.

  Khatun H, Ihara Y, Takakura K, Egashira J, Wada Y, Konno T, Tatemoto H, Yamanaka KI

  Theriogenology ( Theriogenology )  142   131 - 137   2020.01 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Efficient in vitro embryo production using in vivo-matured oocytes from superstimulated Japanese Black cows.

  Egashira J, Ihara Y, Khatun H, Wada Y, Konno T, Tatemoto H, Yamanaka KI

  The Journal of reproduction and development ( 日本繁殖生物学会 )    2019.02

  Type of publication: Research paper (scientific journal)

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  <p>We examined whether the use of <i>in vivo</i>-matured oocytes, collected by ovum pick-up (OPU) from superstimulated Japanese Black cows, can improve the productivity and quality of i<i>n vitro</i> produced embryos. The cows were superstimulated by treatment with progesterone, GnRH, FSH and prostaglandin F2α according to a standardized protocol. The resulting <i>in vivo</i>-matured oocytes were collected by OPU and used subsequently for the other experiments. The immature oocytes from cows in the non-stimulated group were collected by OPU and then subjected to maturation <i>in vitro</i>. We found that the rate of normally distributed cortical granules of the matured oocyte cytoplasm in the superstimulated group was significantly higher than that in the non-stimulated group. The normal cleavage rate (i.e., production of embryos with two equal blastomeres without fragmentation) and freezable blastocyst rate were significantly higher in the superstimulated group than in the non-stimulated group. Among the transferable blastocysts, the ratio of embryos from normal cleavage was also significantly higher in the superstimulated group than in the non-stimulated group. For <i>in vivo</i>-matured oocytes, it was observed that the pregnancy rates were significantly higher when normally cleaved embryos were used for transfer. Taken together, these results suggest that high-quality embryos with respect to developmental kinetics can be efficiently produced with the use of <i>in vivo</i>-matured oocytes collected by OPU from superstimulated Japanese Black cows.</p>

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• Spatio-temporal distribution of eosinophils in the mouse uterus during peri-implantation period.

  Kurane T, Kawase F, Morooka A, Konno T

  Okajimas folia anatomica Japonica ( オカジマ・フォリア・アナトミカ・ヤポニカ編集部 )  96 ( 2 ) 49 - 56   2019 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  <p>Embryo implantation is an immunologically paradoxical event. In humans and rodents, blastocysts adhere to uterine epithelium and then invade into endometrial stroma, while maternal body is protected from extraneous materials by its immune system. Eosinophils, a kind of leucocytes involving parasitic infections and allergic response, increase in number in uterus when serum estrogen level is elevated during estrus cycles. However, response of uterine eosinophils to ovarian estrogen during peri-implantation period is not clear. Therefore, we investigated the distribution of eosinophils in murine peri-implantation uterus. On day 0.5 of pregnancy, eosinophils were found primarily in endometrial stroma near the luminal epithelium, whereas they were primarily distributed in basal endometrium and myometrium on day 3.5 of pregnancy. The number of uterine eosinophils on day 4.5 of pregnancy was significantly increased by inhibition of maternal estrogen action. Collectively, our results indicate that the ovarian estrogen negatively regulates uterine eosinophil distribution during peri-implantation period and provide insight into a role of maternal immune system in embryo implantation.</p>

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• Comparison Study of Allelochemicals and Bispyribac-Sodium on the Germination and Growth Response of Echinochloa crus-galli L.

  Sheikh Muhammad Masum, Mohammad Amzad Hossain, Hikaru Akamine, Jun-Ichi Sakagami, Takahiro Ishii, Toshihiro Konno, Ichiro Nakamura

  Journal of Plant Growth Regulation ( Journal of Plant Growth Regulation )  38 ( 2 ) 501 - 512   2018.10 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  The phytotoxic effects of two allelochemicals (trans-cinnamic acid and syringaldehyde) at different concentrations (1000, 100, 10, and 1 mu M) on seed germination, seedling growth, and physiological and biochemical changes of Echinochloa crus-galli L. were tested by comparison to a commercial herbicide Nominee' (that is, 100g/L bispyribac-sodium). trans-Cinnamic acid and the herbicide inhibited seed germination completely at 100 mu M, whereas for syringaldehyde, complete inhibition required 1000 mu M. However, with 100 mu M syringaldehyde, the seed germination of the test species was 53% of the control. Allelochemicals and the herbicide delayed seed germination and significantly affected the speed of germination index (S), speed of cumulative germination index (AS), and coefficient of germination rate (CRG). The roots were more affected when nutrients were not added to the growth bioassay. In general, with the increasing concentration of allelochemicals from 100 to 1000 mu M, the inhibitory effects increased. Via microscopy analysis, we found leaf blade wilting and necrosis at concentrations above 100 mu M in allelochemical-treated plants. Roots of E. crus-galli treated with 1000 mu M allelochemicals had black points on root nodes but had no root hairs. The anatomy of roots treated with allelochemicals (1000 mu M) showed contraction or reduction of root pith cells as well as fewer and larger vacuoles compared to the control. The allelochemicals also showed remarkable effects on seedling growth, SPAD index, chlorophyll content, and free proline content in a pot culture bioassay, indicating that trans-cinnamic acid and syringaldehyde are potent inhibitors of E. crus-galli growth and can be developed as herbicides for future weed management strategies.

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Presentations 【 display / non-display 】

• Ovarian estrogen negatively regulates uterine eosinophil distribution during implantation window in mice.

  Tomomi Kurane, Akira Morooka, Toshihiro Konno

  OIST and Univ. Ryukyus Joint Symposium  (Okinawa)  2017.10  -  2017.10 

• Mural trophectoderm specific activation of adhesion competence in peri-implantation murine blastocyst

  Ichiko Nishi, Fumie Kawase, Tomomi Murane, Keiichiro Ohta, Hideki Tatemoto, Toshihiro Konno

  4th World Congress of Reproductive Biology  (Okinawa)  2017.09  -  2017.09 

Grant-in-Aid for Scientific Research 【 display / non-display 】

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• Grant-in-Aid for Scientific Research(C)

  Project Year: 2015  -  2017 

  Direct: 3,800,000 (YEN)  Overheads: 1,140,000 (YEN)  Total: 4,940,000 (YEN)