金野　俊洋 (コンノ　トシヒロ)

Konno Toshihiro

写真a

職名

准教授

科研費研究者番号

60568260
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  • 専任   琉球大学   農学部   亜熱帯農林環境科学科   准教授  

  • 併任   琉球大学   農学研究科   亜熱帯農学専攻   准教授  

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  • 　
    -
    2000年03月

    東北大学  農学研究科  博士後期課程  修了

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  • 東北大学 -  博士（農学）  その他 / その他

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  • 2012年12月
    -
    継続中

      琉球大学　農学部　亜熱帯農林環境科学科　准教授  

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  • 1900年04月
    -
    継続中
     

    日本繁殖生物学会

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  • 発生生物学

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  • その他 / その他

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  • 栄養膜細胞分化の制御機構

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  • The strong anti-hyaluronidase effect of ellagic acid markedly decreases polyspermy during in vitro fertilization, resulting in sustainment of the developmental potency in porcine oocytes.

    Kawasaki K, Hirai M, Ishiki Y, Nagahama A, Konno T, Yamanaka K, Tatemoto H

    Theriogenology ( Theriogenology )  215   95 - 102   2024年02月 [ 査読有り ]

    掲載種別: 研究論文（学術雑誌）

    　概要を見る

    The present study investigated the effects of ellagic acid, a type of polyphenol that does not have a glycan and is composed of four hydroxyl groups and two lactone functional groups, on porcine in vitro fertilization (IVF) by focusing on its anti-hyaluronidase activity. A comparative analysis of ellagic acid and apigenin, which is commonly used as a hyaluronidase inhibitor, was performed. It compared the effects of ellagic acid and apigenin on hyaluronidase activity at different concentrations. The results showed that 10, 20, and 40 μM ellagic acid strongly reduced hyaluronidase activity (P < 0.05). The addition of 20 μM ellagic acid, but not apigenin, to porcine IVF medium effectively reduced polyspermy without decreasing sperm penetration or the formation rates of male pronuclei in cumulus-free oocytes. However, neither ellagic acid nor apigenin affected the number of sperm that bound to zona pellucida (ZP) or the induction of zona hardening and protease resistance. The percentage of acrosome-reacting sperm that bound to the ZP was markedly lower in the presence of 20 μM ellagic acid than in the untreated and apigenin-treated groups, even though the antioxidant capacity of ellagic acid was weaker than that of apigenin. Furthermore, a markedly higher percentage of embryos developed to the blastocyst stage in the ellagic acid-treated group, and the apoptotic indexes of expanded blastocysts produced by the ellagic acid treatment during IVF were significantly low. Therefore, the anti-hyaluronidase effect of ellagic acid markedly suppressed the induction of the acrosome reaction in sperm that bound to the ZP, resulting in a marked decrease in polyspermy under conditions that maintained high sperm penetrability during IVF and sustainment of the developmental potency in porcine oocytes.

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  • Re-Evaluation of the Cross-Reactions of the Antibody against the Causative Agent for Paracoccidioidomycosis Ceti; Paracoccidioides ceti and the Related Fungal Species.

    Kanegae H, de Souza Suguiura IM, Tashiro R, Konno T, Hou DX, Sano A, Eto T, Ueda K, Hossain MA

    Microorganisms ( Microorganisms )  11 ( 10 )   2023年09月 [ 査読有り ]

    掲載種別: 研究論文（学術雑誌）

    　概要を見る

    Paracoccidioidomycosis ceti (PCM-C) is a chronic granulomatous keloidal dermatitis in cetaceans that has been reported worldwide and is caused by Paracoccidioides ceti. Serological cross-reactions among highly pathogenic fungal infections and related diseases have been reported. However, the true cross-reaction of antibodies against P. ceti has remained unknown due to the use of positive control sera from infected dolphins. This study aimed to re-evaluate antibodies from mechanically dislodged fungal cells in the infected tissue of a PCM-C case and demonstrate the actual cross-reaction. The results revealed a limited cross-reaction between PCM-C and paracoccidioidomycosis, while the antibodies did not react with other pathogens such as Coccidioides posadasii, Histoplasama capsulatum, and Arthrographis kalrae. Thus, the method for evaluation of the antibody against PCM-C is reliable, and there is potential for epidemiological study.

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  • Antifreeze protein type III addition to freezing extender comprehensively improves post-thaw sperm properties in Okinawan native Agu pig.

    Masuda Y, Kheawkanha T, Nagahama A, Kawasaki K, Konno T, Yamanaka K, Tatemoto H

    Animal reproduction science ( Animal Reproduction Science )  252   107232 - 107232   2023年05月 [ 査読有り ]

    掲載種別: 研究論文（学術雑誌）

    　概要を見る

    Sperm cryopreservation often leads to physical cell damage through ice crystal formation. This study evaluates the improvements to freezing extender cryoprotective activity due to antifreeze protein (AFP) addition, which primarily acts on ice crystal formation, through investigating the post-thaw sperm properties of Okinawan native Agu pig. Six individual boar sperm samples were diluted with the freezing extender supplemented with 1 μg/mL of AFP I or AFP III and then subjected to cryopreservation. Treatment with AFP I during the freezing procedure had no improvement for any characteristics after thawing compared to untreated sperm. In contrast, the addition of AFP III to the freezing extender strongly increased sperm motility, mitochondria and cell membrane integrity, and the acrosomal proteolytic activity of frozen-thawed sperm in 5 of 6 individuals (P < 0.05). Furthermore, cryoinjury prevention by AFP III significantly enhanced sperm viability (by ATP content), and maintained DNA quality and in vitro sperm penetrability compared with AFP I treatment (P < 0.05). These findings demonstrate that AFP III addition to the freezing extender of boar sperm is more effective in maintaining sperm characteristics than the extender without AFP III or AFP I, despite individual differences in response.

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  • Addition of l-carnitine to the freezing extender improves post-thaw sperm quality of Okinawan native Agu pig.

    Tatemoto H, Osokoshi N, Hirai M, Masuda Y, Konno T, Yamanaka K

    Theriogenology ( Theriogenology )  188   170 - 176   2022年08月 [ 査読有り ]

    掲載種別: 研究論文（学術雑誌）

    　概要を見る

    The objective of the present study was to establish whether the addition of l-carnitine (LC), which exhibits antioxidant activity, to the freezing extender improves the quality of cryopreserved Okinawan native Agu pig sperm. Ejaculated sperm frozen in an extender supplemented with 0, 1, 2.5, or 5 mM LC was thawed, and the integrities of mitochondria and the plasmalemma and other sperm characteristics were evaluated. The treatment with different concentrations of LC effectively improved sperm motility, mitochondrial and plasmalemmal integrities, and the proteolytic activity of acrosomal contents after freeze-thawing (P < 0.05). The proportion of post-thaw sperm possessing intact mitochondria and plasmalemma and higher proteolytic activity of acrosomal contents was markedly higher among sperm frozen in the presence of 2.5 mM LC than among sperm frozen in the extender without LC (P < 0.05). Furthermore, although the addition of LC to the freezing extender had no effect on disturbance of DNA damage and caspase activity, sperm treated with 2.5 mM LC during freezing exhibited significantly higher penetrability into matured oocytes in vitro than untreated sperm. Collectively, these results indicate that the addition of LC to the freezing extender effectively improved the post-thaw quality of Agu pig sperm by preventing mitochondrial dysfunction caused by oxidative stress during cryopreservation.

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  • Endoplasmic reticulum stress attenuation promotes bovine oocyte maturation in vitro.

    Khatun H, Wada Y, Konno T, Tatemoto H, Yamanaka KI

    Reproduction (Cambridge, England) ( Reproduction (Cambridge, England) )  159 ( 4 ) 361 - 370   2020年04月 [ 査読有り ]

    掲載種別: 研究論文（学術雑誌）

    　概要を見る

    We have previously reported that regulation of endoplasmic reticulum (ER) stress during in vitro culture acutely increases bovine embryo developmental rate and cryotolerance; these data indicate that ER stress is a critical factor reducing the quality of in vitro-produced embryos. In the current follow-up study, we examined whether ER stress attenuation during in vitro maturation influences meiotic maturation, oocyte quality, and subsequent embryonic development. Bovine cumulus oocyte complexes (COCs) derived from slaughterhouse ovaries were matured with or without tauroursodeoxycholic acid (TUDCA), a selective inhibitor of ER stress (0, 50, 100, and 200 µM) for 22 h followed by in vitro fertilization, and zygotes were cultured for 8 days. Of the different doses of TUDCA, 100 μM TUDCA significantly increased the maturation rate, and decreased reactive oxygen species in denuded oocytes, and appeared lower number of apoptotic cells in matured COCs. Subsequently, treatment of TUDCA (100 µM) decreased the localization and amount of GRP78/BIP protein level as well as ER stress (GRP78/BIP, PERK, IER1, ATF4, and XBP1) and apoptosis (CHOP and BAX)-related gene expression, while it increased the anti-apoptotic gene BCL2 level in matured COCs. Moreover, addition of TUDCA (100 µM) during IVM significantly improved the blastocyst formation rate (43.6 ± 1.8% vs 49.7 ± 1.3%) and decreased the number of apoptotic cells (7.7 ± 1.1% vs 5.03 ± 0.6%) in blastocysts. These findings suggest that the presence of ER stress during maturation impairs the developmental competence of bovine COCs and that this process can be reversed by TUDCA.

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  • Ovarian estrogen negatively regulates uterine eosinophil distribution during implantation window in mice.

    Tomomi Kurane, Akira Morooka, Toshihiro Konno

    OIST and Univ. Ryukyus Joint Symposium  (Okinawa)  2017年10月  -  2017年10月   

  • Mural trophectoderm specific activation of adhesion competence in peri-implantation murine blastocyst

    Ichiko Nishi, Fumie Kawase, Tomomi Murane, Keiichiro Ohta, Hideki Tatemoto, Toshihiro Konno

    4th World Congress of Reproductive Biology  (Okinawa)  2017年09月  -  2017年09月   

  • 精液輸送ならびに精子凍結時のTrolox処理が沖縄在来豚アグー精子の融解後の性状改善に及ぼす効果

    建本秀樹, 島田晴加, 金野俊洋

    第8回日本暖地畜産学会熊本大会  2015年  -  2015年   

  • 野生オオコウモリによる外傷後，狂犬病暴露後ワクチンを接種した1事例

    川瀬史恵，山口さやか，佐野文子，金野俊洋，兼島　孝，玉城佑一郎，上里博

    第14回人と動物の共通感染症研究会学術集会  2014年  -  2014年   

  • Epithelial-mesenchymal transition of trophectoderm cells during embryo implantation

    Konno T, Yamokoshi S, Bai R, Chaen T, Egashira M, Nomura N, Sakurai T, Imakawa K

    第104回日本繁殖生物学会大会併催国際シンポジウム  2011年  -  2011年   

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  • 胎盤免疫とリンハ?球:妊娠維持と感染防御の新しい生殖免疫 学領域の開拓

    研究費種類: 学内助成（後援会・財団含む）  参画方法: その他

    研究種別: その他  事業名: -

    研究期間: 2015年  -  2015年 

    代表者: 金野俊洋  連携研究者: 松﨑吾朗, 梅村正幸  資金配分機関: -

    直接経費: 880,000（円）  金額合計: 880,000（円）

  • 子宮内における胚発生誘導機序に関する基礎的研究

    研究費種類: 財団・社団法人等の民間助成金  参画方法: その他

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    研究期間: 2014年  -  2014年 

    代表者: 金野俊洋  資金配分機関: -

    直接経費: 300,000（円）  金額合計: 300,000（円）

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