金野 俊洋 (コンノ トシヒロ)

Konno Toshihiro

写真a

職名

准教授

科研費研究者番号

60568260

現在の所属組織 【 表示 / 非表示

  • 専任   琉球大学   農学部   亜熱帯農林環境科学科   准教授  

  • 併任   琉球大学   農学研究科   亜熱帯農学専攻   准教授  

出身大学院 【 表示 / 非表示

  •  
    -
    2000年03月

    東北大学  農学研究科  博士後期課程  修了

取得学位 【 表示 / 非表示

  • 東北大学 -  博士(農学)  その他 / その他

職歴 【 表示 / 非表示

  • 2012年12月
    -
    継続中

      琉球大学 農学部 亜熱帯農林環境科学科 准教授  

所属学会・委員会 【 表示 / 非表示

  • 1900年04月
    -
    継続中
     

    日本繁殖生物学会

研究キーワード 【 表示 / 非表示

  • 発生生物学

研究分野 【 表示 / 非表示

  • その他 / その他

主たる研究テーマ 【 表示 / 非表示

  • 栄養膜細胞分化の制御機構

論文 【 表示 / 非表示

  • Melatonin improves the in vitro growth of bovine oocytes collected from early antral follicles by maintaining oocyte-cumulus cell communication.

    Islam MN, Ebara F, Konno T, Tatemoto H, Yamanaka KI

    Reproductive medicine and biology   24 ( 1 ) e12629   2025年01月 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

  • Adolescent social isolation disrupts developmental tuning of neuropeptide circuits in the hypothalamus to amygdala regulating social and defensive behavior.

    Arakawa H, Tokashiki M, Higuchi Y, Konno T

    Peptides ( Peptides )  175   171178   2024年05月 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

  • The strong anti-hyaluronidase effect of ellagic acid markedly decreases polyspermy during in vitro fertilization, resulting in sustainment of the developmental potency in porcine oocytes.

    Kawasaki K, Hirai M, Ishiki Y, Nagahama A, Konno T, Yamanaka K, Tatemoto H

    Theriogenology ( Theriogenology )  215   95 - 102   2024年02月 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

     概要を見る

    The present study investigated the effects of ellagic acid, a type of polyphenol that does not have a glycan and is composed of four hydroxyl groups and two lactone functional groups, on porcine in vitro fertilization (IVF) by focusing on its anti-hyaluronidase activity. A comparative analysis of ellagic acid and apigenin, which is commonly used as a hyaluronidase inhibitor, was performed. It compared the effects of ellagic acid and apigenin on hyaluronidase activity at different concentrations. The results showed that 10, 20, and 40 μM ellagic acid strongly reduced hyaluronidase activity (P < 0.05). The addition of 20 μM ellagic acid, but not apigenin, to porcine IVF medium effectively reduced polyspermy without decreasing sperm penetration or the formation rates of male pronuclei in cumulus-free oocytes. However, neither ellagic acid nor apigenin affected the number of sperm that bound to zona pellucida (ZP) or the induction of zona hardening and protease resistance. The percentage of acrosome-reacting sperm that bound to the ZP was markedly lower in the presence of 20 μM ellagic acid than in the untreated and apigenin-treated groups, even though the antioxidant capacity of ellagic acid was weaker than that of apigenin. Furthermore, a markedly higher percentage of embryos developed to the blastocyst stage in the ellagic acid-treated group, and the apoptotic indexes of expanded blastocysts produced by the ellagic acid treatment during IVF were significantly low. Therefore, the anti-hyaluronidase effect of ellagic acid markedly suppressed the induction of the acrosome reaction in sperm that bound to the ZP, resulting in a marked decrease in polyspermy under conditions that maintained high sperm penetrability during IVF and sustainment of the developmental potency in porcine oocytes.

  • Re-Evaluation of the Cross-Reactions of the Antibody against the Causative Agent for Paracoccidioidomycosis Ceti; Paracoccidioides ceti and the Related Fungal Species.

    Kanegae H, de Souza Suguiura IM, Tashiro R, Konno T, Hou DX, Sano A, Eto T, Ueda K, Hossain MA

    Microorganisms ( Microorganisms )  11 ( 10 )   2023年09月 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

     概要を見る

    Paracoccidioidomycosis ceti (PCM-C) is a chronic granulomatous keloidal dermatitis in cetaceans that has been reported worldwide and is caused by Paracoccidioides ceti. Serological cross-reactions among highly pathogenic fungal infections and related diseases have been reported. However, the true cross-reaction of antibodies against P. ceti has remained unknown due to the use of positive control sera from infected dolphins. This study aimed to re-evaluate antibodies from mechanically dislodged fungal cells in the infected tissue of a PCM-C case and demonstrate the actual cross-reaction. The results revealed a limited cross-reaction between PCM-C and paracoccidioidomycosis, while the antibodies did not react with other pathogens such as Coccidioides posadasii, Histoplasama capsulatum, and Arthrographis kalrae. Thus, the method for evaluation of the antibody against PCM-C is reliable, and there is potential for epidemiological study.

  • Antifreeze protein type III addition to freezing extender comprehensively improves post-thaw sperm properties in Okinawan native Agu pig.

    Masuda Y, Kheawkanha T, Nagahama A, Kawasaki K, Konno T, Yamanaka K, Tatemoto H

    Animal reproduction science ( Animal Reproduction Science )  252   107232 - 107232   2023年05月 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

     概要を見る

    Sperm cryopreservation often leads to physical cell damage through ice crystal formation. This study evaluates the improvements to freezing extender cryoprotective activity due to antifreeze protein (AFP) addition, which primarily acts on ice crystal formation, through investigating the post-thaw sperm properties of Okinawan native Agu pig. Six individual boar sperm samples were diluted with the freezing extender supplemented with 1 μg/mL of AFP I or AFP III and then subjected to cryopreservation. Treatment with AFP I during the freezing procedure had no improvement for any characteristics after thawing compared to untreated sperm. In contrast, the addition of AFP III to the freezing extender strongly increased sperm motility, mitochondria and cell membrane integrity, and the acrosomal proteolytic activity of frozen-thawed sperm in 5 of 6 individuals (P < 0.05). Furthermore, cryoinjury prevention by AFP III significantly enhanced sperm viability (by ATP content), and maintained DNA quality and in vitro sperm penetrability compared with AFP I treatment (P < 0.05). These findings demonstrate that AFP III addition to the freezing extender of boar sperm is more effective in maintaining sperm characteristics than the extender without AFP III or AFP I, despite individual differences in response.

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著書 【 表示 / 非表示

研究発表等の成果普及活動 【 表示 / 非表示

  • Localization and characteristics of IL-17F in the mycobacteria-infected lungs

    Masayuki Umemura, Toshihiro Konno, Giichi Takaesu, Goro Matsuzaki

    第53回 日本免疫学会学術集会  (長崎市)  2024年12月  -  2024年12月   

  • Ovarian estrogen negatively regulates uterine eosinophil distribution during implantation window in mice.

    Tomomi Kurane, Akira Morooka, Toshihiro Konno

    OIST and Univ. Ryukyus Joint Symposium  (Okinawa)  2017年10月  -  2017年10月   

  • Mural trophectoderm specific activation of adhesion competence in peri-implantation murine blastocyst

    Ichiko Nishi, Fumie Kawase, Tomomi Murane, Keiichiro Ohta, Hideki Tatemoto, Toshihiro Konno

    4th World Congress of Reproductive Biology  (Okinawa)  2017年09月  -  2017年09月   

  • 精液輸送ならびに精子凍結時のTrolox処理が沖縄在来豚アグー精子の融解後の性状改善に及ぼす効果

    建本秀樹, 島田晴加, 金野俊洋

    第8回日本暖地畜産学会熊本大会  2015年  -  2015年   

  • 野生オオコウモリによる外傷後,狂犬病暴露後ワクチンを接種した1事例

    川瀬史恵,山口さやか,佐野文子,金野俊洋,兼島 孝,玉城佑一郎,上里博

    第14回人と動物の共通感染症研究会学術集会  2014年  -  2014年   

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科研費獲得情報 【 表示 / 非表示

その他研究費獲得情報 【 表示 / 非表示

  • 胎盤免疫とリンハ?球:妊娠維持と感染防御の新しい生殖免疫 学領域の開拓

    研究費種類: 学内助成(後援会・財団含む)  参画方法: その他

    研究種別: その他  事業名: -

    研究期間: 2015年  -  2015年 

    代表者: 金野俊洋  連携研究者: 松﨑吾朗, 梅村正幸  資金配分機関: -

    直接経費: 880,000(円)  金額合計: 880,000(円)

  • 子宮内における胚発生誘導機序に関する基礎的研究

    研究費種類: 財団・社団法人等の民間助成金  参画方法: その他

    研究種別: その他  事業名: -

    研究期間: 2014年  -  2014年 

    代表者: 金野俊洋  資金配分機関: -

    直接経費: 300,000(円)  金額合計: 300,000(円)