金野 俊洋 (コンノ トシヒロ)

Konno Toshihiro

写真a

職名

准教授

科研費研究者番号

60568260

現在の所属組織 【 表示 / 非表示

  • 専任   琉球大学   農学部   亜熱帯農林環境科学科   准教授  

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  •  
    -
    2000年03月

    東北大学  農学研究科  博士後期課程  修了

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  • 東北大学 -  博士(農学)  その他 / その他

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  • 2012年12月
    -
    継続中

      琉球大学 農学部 亜熱帯農林環境科学科 准教授  

所属学会・委員会 【 表示 / 非表示

  • 1900年04月
    -
    継続中
     

    日本繁殖生物学会

研究キーワード 【 表示 / 非表示

  • 発生生物学

研究分野 【 表示 / 非表示

  • その他 / その他

主たる研究テーマ 【 表示 / 非表示

  • 栄養膜細胞分化の制御機構

論文 【 表示 / 非表示

  • Endoplasmic reticulum stress attenuation promotes bovine oocyte maturation in vitro.

    Khatun H, Wada Y, Konno T, Tatemoto H, Yamanaka KI

    Reproduction (Cambridge, England) ( Reproduction (Cambridge, England) )  159 ( 4 ) 361 - 370   2020年04月 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

  • Role of endoplasmic reticulum stress on developmental competency and cryo-tolerance in bovine embryos.

    Khatun H, Ihara Y, Takakura K, Egashira J, Wada Y, Konno T, Tatemoto H, Yamanaka KI

    Theriogenology ( Theriogenology )  142   131 - 137   2020年01月 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

  • Efficient in vitro embryo production using in vivo-matured oocytes from superstimulated Japanese Black cows.

    Egashira J, Ihara Y, Khatun H, Wada Y, Konno T, Tatemoto H, Yamanaka KI

    The Journal of reproduction and development ( 日本繁殖生物学会 )    2019年02月

    掲載種別: 研究論文(学術雑誌)

     概要を見る

    <p>We examined whether the use of <i>in vivo</i>-matured oocytes, collected by ovum pick-up (OPU) from superstimulated Japanese Black cows, can improve the productivity and quality of i<i>n vitro</i> produced embryos. The cows were superstimulated by treatment with progesterone, GnRH, FSH and prostaglandin F2α according to a standardized protocol. The resulting <i>in vivo</i>-matured oocytes were collected by OPU and used subsequently for the other experiments. The immature oocytes from cows in the non-stimulated group were collected by OPU and then subjected to maturation <i>in vitro</i>. We found that the rate of normally distributed cortical granules of the matured oocyte cytoplasm in the superstimulated group was significantly higher than that in the non-stimulated group. The normal cleavage rate (i.e., production of embryos with two equal blastomeres without fragmentation) and freezable blastocyst rate were significantly higher in the superstimulated group than in the non-stimulated group. Among the transferable blastocysts, the ratio of embryos from normal cleavage was also significantly higher in the superstimulated group than in the non-stimulated group. For <i>in vivo</i>-matured oocytes, it was observed that the pregnancy rates were significantly higher when normally cleaved embryos were used for transfer. Taken together, these results suggest that high-quality embryos with respect to developmental kinetics can be efficiently produced with the use of <i>in vivo</i>-matured oocytes collected by OPU from superstimulated Japanese Black cows.</p>

  • Spatio-temporal distribution of eosinophils in the mouse uterus during peri-implantation period.

    Kurane T, Kawase F, Morooka A, Konno T

    Okajimas folia anatomica Japonica ( オカジマ・フォリア・アナトミカ・ヤポニカ編集部 )  96 ( 2 ) 49 - 56   2019年 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

     概要を見る

    <p>Embryo implantation is an immunologically paradoxical event. In humans and rodents, blastocysts adhere to uterine epithelium and then invade into endometrial stroma, while maternal body is protected from extraneous materials by its immune system. Eosinophils, a kind of leucocytes involving parasitic infections and allergic response, increase in number in uterus when serum estrogen level is elevated during estrus cycles. However, response of uterine eosinophils to ovarian estrogen during peri-implantation period is not clear. Therefore, we investigated the distribution of eosinophils in murine peri-implantation uterus. On day 0.5 of pregnancy, eosinophils were found primarily in endometrial stroma near the luminal epithelium, whereas they were primarily distributed in basal endometrium and myometrium on day 3.5 of pregnancy. The number of uterine eosinophils on day 4.5 of pregnancy was significantly increased by inhibition of maternal estrogen action. Collectively, our results indicate that the ovarian estrogen negatively regulates uterine eosinophil distribution during peri-implantation period and provide insight into a role of maternal immune system in embryo implantation.</p>

  • Comparison Study of Allelochemicals and Bispyribac-Sodium on the Germination and Growth Response of Echinochloa crus-galli L.

    Sheikh Muhammad Masum, Mohammad Amzad Hossain, Hikaru Akamine, Jun-Ichi Sakagami, Takahiro Ishii, Toshihiro Konno, Ichiro Nakamura

    Journal of Plant Growth Regulation ( Journal of Plant Growth Regulation )  38 ( 2 ) 501 - 512   2018年10月 [ 査読有り ]

    掲載種別: 研究論文(学術雑誌)

     概要を見る

    The phytotoxic effects of two allelochemicals (trans-cinnamic acid and syringaldehyde) at different concentrations (1000, 100, 10, and 1 mu M) on seed germination, seedling growth, and physiological and biochemical changes of Echinochloa crus-galli L. were tested by comparison to a commercial herbicide Nominee' (that is, 100g/L bispyribac-sodium). trans-Cinnamic acid and the herbicide inhibited seed germination completely at 100 mu M, whereas for syringaldehyde, complete inhibition required 1000 mu M. However, with 100 mu M syringaldehyde, the seed germination of the test species was 53% of the control. Allelochemicals and the herbicide delayed seed germination and significantly affected the speed of germination index (S), speed of cumulative germination index (AS), and coefficient of germination rate (CRG). The roots were more affected when nutrients were not added to the growth bioassay. In general, with the increasing concentration of allelochemicals from 100 to 1000 mu M, the inhibitory effects increased. Via microscopy analysis, we found leaf blade wilting and necrosis at concentrations above 100 mu M in allelochemical-treated plants. Roots of E. crus-galli treated with 1000 mu M allelochemicals had black points on root nodes but had no root hairs. The anatomy of roots treated with allelochemicals (1000 mu M) showed contraction or reduction of root pith cells as well as fewer and larger vacuoles compared to the control. The allelochemicals also showed remarkable effects on seedling growth, SPAD index, chlorophyll content, and free proline content in a pot culture bioassay, indicating that trans-cinnamic acid and syringaldehyde are potent inhibitors of E. crus-galli growth and can be developed as herbicides for future weed management strategies.

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研究発表等の成果普及活動 【 表示 / 非表示

  • Ovarian estrogen negatively regulates uterine eosinophil distribution during implantation window in mice.

    Tomomi Kurane, Akira Morooka, Toshihiro Konno

    OIST and Univ. Ryukyus Joint Symposium  (Okinawa)  2017年10月  -  2017年10月   

  • Mural trophectoderm specific activation of adhesion competence in peri-implantation murine blastocyst

    Ichiko Nishi, Fumie Kawase, Tomomi Murane, Keiichiro Ohta, Hideki Tatemoto, Toshihiro Konno

    4th World Congress of Reproductive Biology  (Okinawa)  2017年09月  -  2017年09月   

  • 精液輸送ならびに精子凍結時のTrolox処理が沖縄在来豚アグー精子の融解後の性状改善に及ぼす効果

    建本秀樹, 島田晴加, 金野俊洋

    第8回日本暖地畜産学会熊本大会  2015年  -  2015年   

  • 野生オオコウモリによる外傷後,狂犬病暴露後ワクチンを接種した1事例

    川瀬史恵,山口さやか,佐野文子,金野俊洋,兼島 孝,玉城佑一郎,上里博

    第14回人と動物の共通感染症研究会学術集会  2014年  -  2014年   

  • Epithelial-mesenchymal transition of trophectoderm cells during embryo implantation

    Konno T, Yamokoshi S, Bai R, Chaen T, Egashira M, Nomura N, Sakurai T, Imakawa K

    第104回日本繁殖生物学会大会併催国際シンポジウム  2011年  -  2011年   

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科研費獲得情報 【 表示 / 非表示

その他研究費獲得情報 【 表示 / 非表示

  • 胎盤免疫とリンハ?球:妊娠維持と感染防御の新しい生殖免疫 学領域の開拓

    研究費種類: 学内助成(後援会・財団含む)  参画方法: その他

    研究種別: その他  事業名: -

    研究期間: 2015年  -  2015年 

    代表者: 金野俊洋  連携研究者: 松﨑吾朗, 梅村正幸  資金配分機関: -

    直接経費: 880,000(円)  金額合計: 880,000(円)

  • 子宮内における胚発生誘導機序に関する基礎的研究

    研究費種類: 財団・社団法人等の民間助成金  参画方法: その他

    研究種別: その他  事業名: -

    研究期間: 2014年  -  2014年 

    代表者: 金野俊洋  資金配分機関: -

    直接経費: 300,000(円)  金額合計: 300,000(円)