Current Affiliation Organization 【 display / non-display 】

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• Screening of Cancer Promotion Inhibiting Activity from Food Material

• Tumor Selective Cytotoxicity and Functional Analysis of Medical Herb Extract

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Published Papers 【 display / non-display 】

• Stress amelioration and anti-inflammatory potential of Shiikuwasha (Citrus depressa Hayata) essential oil, limonene, and γ-terpinene

  Y Asikin, K Shimizu, H Iwasaki, H Oku, K Wada

  Journal of Food and Drug Analysis ( Journal of Food and Drug Analysis )  30 ( 3 ) 454 - 465   2022.09 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• The dose-dependent pteryxin-mediated molecular mechanisms in suppressing adipogenesis in vitro

  Ruwani N Nugara, Hirosuke Oku, Seiko Saitoh, Masashi Inafuku, Hironori Iwasaki, RP Kumara, LJS Undugoda, BNS Lankasena

  Journal of Functional Foods ( Journal of Functional Foods )  82   2021.07 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Molecular cloning of putative chloroplastic cysteine synthase in Leucaena leucocephala.

  Harun-Ur-Rashid M, Oogai S, Parveen S, Inafuku M, Iwasaki H, Fukuta M, Amzad Hossain M, Oku H

  Journal of plant research ( Journal of Plant Research )  133 ( 1 ) 95 - 108   2020.01 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

  　View Summary

  Cysteine biosynthesis is directed by the successive commitments of serine acetyltransferase, and O-acetylserine (thiol) lyase (OASTL) compounds, which subsequently frame the decameric cysteine synthase complex. The isoforms of OASTL are found in three compartments of the cell: the cytosol, plastid, and mitochondria. In this investigation, we first isolated putative chloroplastic OASTL (Ch-OASTL) from Leucaena leucocephala, and the Ch-OASTL was then expressed in BL21-competent Escherichia coli. The putative Ch-OASTL cDNA clone had 1,543 base pairs with 391 amino acids in its open reading frame and a molecular weight of 41.54 kDa. The purified protein product exhibited cysteine synthesis ability, but not mimosine synthesis activity. However, they both make the common α-aminoacrylate intermediate in their first half reaction scheme with the conventional substrate O-acetyl serine (OAS). Hence, we considered putative Ch-OASTL a cysteine-specific enzyme. Kinetic studies demonstrated that the optimum pH for cysteine synthesis was 7.0, and the optimum temperature was 40 °C. In the cysteine synthesis assay, the Km and kcat values were 838 ± 26 µM and 72.83 s-1 for OAS, respectively, and 60 ± 2 µM and 2.43 s-1 for Na2S, respectively. We can infer that putative Ch-OASTL regulatory role is considered a sensor for sulfur constraint conditions, and it acts as a forerunner of various metabolic compound molecules.

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• Molecular regulatory mechanism of isoprene emission under short-term drought stress in the tropical tree Ficus septica.

  Parveen S, Rashid MH, Inafuku M, Iwasaki H, Oku H

  Tree physiology ( Tree Physiology )  39 ( 3 ) 440 - 453   2018.11 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

  　View Summary

  Isoprene is emitted by many plants and is thought to function as an antioxidant under stressful conditions. However, the detailed regulatory mechanism of isoprene emission in relation to the antioxidant system remains unclear. Therefore, in this study, we explored the molecular regulatory mechanism of isoprene emission under short-term drought stress in the tropical tree Ficus septica Burm.f. We found that the soil moisture content gradually decreased from 55% on Day 1 (D1) to 23% (wilting point) on D5 after withholding water for 4 days and then returning to the initial level following re-watering on D6. On D5, drought-stressed plants had more than twofold higher isoprene emission and 90.6% lower photosynthesis rates, 99.5% lower stomatal conductance and 82.3% lower transpiration rates than well-watered control plants. It was also estimated that the isoprene concentration inside the leaf greatly increased on D5 due to the increased isoprene emission rate and reduced stomatal conductance. Among the traits related to the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway, which is responsible for isoprene biosynthesis, the isoprene synthase (IspS) protein level was positively correlated with the isoprene emission rate in stressed plants. The transcripts of the antioxidant genes peroxidase 2 (POD2), POD4, copper-zinc superoxide dismutase 2 (Cu-ZnSOD2) and manganese superoxide dismutase 1 (Mn-SOD1) also increased during the drying period, while those of ascorbate peroxidase 1 (APX1) decreased. However, there was only a weak correlation between isoprene emission and antioxidant enzyme gene expression, indicating that the regulation of isoprene biosynthesis is not directly linked to the antioxidant defense network in drought-stressed F. septica. These findings suggest that the post-transcriptional regulation of IspS led to the observed change in isoprene emission rate, which enhanced the quenching of reactive oxygen species (ROS) and, in combination with the increased antioxidant enzyme activity, conferred tolerance to drought stress in this species.

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• Cytosolic Cysteine Synthase Switch Cysteine and Mimosine Production in Leucaena leucocephala.

  Md Harun-Ur-Rashid, Iwasaki H, Parveen S, Oogai S, Fukuta M, Hossain MA, Anai T, Oku H

  Applied biochemistry and biotechnology ( Applied Biochemistry and Biotechnology )  186 ( 3 ) 613 - 632   2018.11 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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Other Papers 【 display / non-display 】

Other Papers 【 display / non-display 】

• 沖縄県与那国町産ボタンボウフウ(長命草)の抗メタボリックシンドローム作用とその寄与成分

  稲福征志, 岩崎公典, 大野智, 坂上未紀, 屋宏典

  日本栄養・食糧学会大会講演要旨集   71st   167   2017.04

   

  J-GLOBAL

• Camptothecin耐性癌細胞に対する植物アルカロイドNitidineの効果

  平良直幸, 岩崎公典, 稲福征志, 屋宏典

  日本栄養・食糧学会九州・沖縄支部大会講演要旨集   2015   81   2015.10

   

  J-GLOBAL

Grant-in-Aid for Scientific Research 【 display / non-display 】

Grant-in-Aid for Scientific Research 【 display / non-display 】

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2020  -  2023 

  Direct: 0 (YEN)  Overheads: 0 (YEN)  Total: 0 (YEN)

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2011  -  2013 

  Direct: 0 (YEN)  Overheads: 0 (YEN)  Total: 0 (YEN)

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2008  -  2010 

  Direct: 3,200,000 (YEN)  Overheads: 960,000 (YEN)  Total: 4,160,000 (YEN)