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• Interference with the interaction between sperm arylsulfatase A and sulfated carbohydrate chain residues of the zona pellucida effectively suppresses polyspermy during in vitro fertilization of porcine oocytes

  Sakamura S, Kawasaki K, Yamanaka K, Tatemoto H

  Theriogenology ( ELSEVIER )  240   117396   2025.07 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Melatonin improves the in vitro growth of bovine oocytes collected from early antral follicles by maintaining oocyte-cumulus cell communication

  65) Islam MN, Ebara F, Konno T, Tatemoto H, Yamanaka K.

  Reproductive Medicine and Biology ( WILEY )  24 ( 1 ) e1269   2025.02 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  Abstract Purpose In vitro, oocyte development is susceptible to oxidative stress, which leads to endoplasmic reticulum (ER) stress. This study investigated whether the antioxidant melatonin attenuates ER stress and maintains oocyte‐cumulus cell communication during the in vitro growth (IVG) of bovine oocytes. Methods Oocyte‐granulosa cell complexes (OGCs) were harvested from slaughterhouse‐derived ovaries and grown in vitro for 5 d at 38.5°C in 5% CO₂ humidified air. Melatonin (10⁻⁷, 10⁻⁹, or 10⁻¹¹ M) was added to the culture medium. Results Oocyte diameter increased on day 5 from its initial value in all groups. The antrum formation rate was significantly higher in the 10⁻⁹ M melatonin‐treated group than in the control. The melatonin‐treated group showed reduced oxidative stress and increased gap junction communication compared with the control. ER stress‐related genes in OGCs were significantly downregulated in the 10⁻⁹ M melatonin‐treated group compared with those in the control. No significant changes were found in subsequent maturation among groups; however, 10⁻⁹ M melatonin treatment during IVG and IVM increased the maturation rate compared with that in the control. Conclusions Melatonin reduces oxidative stress, which attenuates ER stress in OGCs during IVG of bovine oocytes and may improve IVG efficiency in assisted reproductive technology.

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• Attenuation of endoplasmic reticulum stress improves in vitro growth and subsequent maturation of bovine oocyte

  Islam MN, Ebara F, Kawasaki K, Konno T, Tatemoto H, Yamanaka K.

  Theriogenology ( ELSEVIER )  288   51 - 63   2024.10 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• The strong anti-hyaluronidase effect of ellagic acid markedly decreasespolyspermy during in vitro fertilization, resulting in sustainment of thedevelopmental potency in porcine oocytes

  Kawasaki K, Hirai M, Ishiki Y, Nagahama A, Konno T, Tamanaka K, Tatemoto H

  Theriogenology ( Elsevier )  215   95 - 102   2024.02 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• The strong anti-hyaluronidase effect of ellagic acid markedly decreases polyspermy during<i> in</i><i> vitro</i> fertilization, resulting in sustainment of the developmental potency in porcine oocytes

  Kawasaki, K; Hirai, M; Ishiki, Y; Nagahama, A; Konno, T; Yamanaka, K; Tatemoto, H

  THERIOGENOLOGY ( Elsevier )  215   95 - 102   2024.02

  Type of publication: Research paper (scientific journal)

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  The present study investigated the effects of ellagic acid, a type of polyphenol that does not have a glycan and is composed of four hydroxyl groups and two lactone functional groups, on porcine in vitro fertilization (IVF) by focusing on its anti-hyaluronidase activity. A comparative analysis of ellagic acid and apigenin, which is commonly used as a hyaluronidase inhibitor, was performed. It compared the effects of ellagic acid and apigenin on hyaluronidase activity at different concentrations. The results showed that 10, 20, and 40 μM ellagic acid strongly reduced hyaluronidase activity (P < 0.05). The addition of 20 μM ellagic acid, but not apigenin, to porcine IVF medium effectively reduced polyspermy without decreasing sperm penetration or the formation rates of male pronuclei in cumulus-free oocytes. However, neither ellagic acid nor apigenin affected the number of sperm that bound to zona pellucida (ZP) or the induction of zona hardening and protease resistance. The percentage of acrosome-reacting sperm that bound to the ZP was markedly lower in the presence of 20 μM ellagic acid than in the untreated and apigenin-treated groups, even though the antioxidant capacity of ellagic acid was weaker than that of apigenin. Furthermore, a markedly higher percentage of embryos developed to the blastocyst stage in the ellagic acid-treated group, and the apoptotic indexes of expanded blastocysts produced by the ellagic acid treatment during IVF were significantly low. Therefore, the anti-hyaluronidase effect of ellagic acid markedly suppressed the induction of the acrosome reaction in sperm that bound to the ZP, resulting in a marked decrease in polyspermy under conditions that maintained high sperm penetrability during IVF and sustainment of the developmental potency in porcine oocytes.

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• Efficient in vitro embryo production system using in vivo-matured oocytes from superstimulated Japanese black cows

  Egashira J, Tatemoto H, Wada Y, Yamanaka K

  Proceedings of the Annual Conference of the International Embryo Transfer Society  (New Orleans, Louisiana, USA)  2019.01  -  2019.01 

• Mural trophectoderm specific activation of adhesion competence in peri-implantation murine blastocyst

  Nishi I, Kawase F, Kurane T, Ohta K, Tatemoto H, Konno T

  4th World Congress of Reproductive Biology  (Okinawa Convention Center)  2017.09  -  2017.09 

• Improvement in the post-thaw qualities of Okinawan native Agu pig spermatozoa treated with skim milk and Trolox prior to cryopreservation

  Tatemoto H, Mitsube R, Tokeshi I, Uehara M, Sadoyama Y, Funabiki M, Konno T

  The 17th AAAP Animal Science Congress  (Fukuoka)  2016.08  -  2016.08 

• Improvement of the post-thaw qualities of Okinawan native pig Agu spermatozoa after addition of AA-2G and LDL to freezing extender

  Tatemoto H, Nakamura S, Ashizawa K, Yoshimoto T, Yamauchi S, Muto N, Nakada T

  11th International Symposium on Spermatology  2010.07  -  2010.07 

• Sperm motility modulation of Ijima&acute;s copper pheasants (Syrmaticus soemmerringii ijimae), one of "near threatened" species in Japan

  Ashizawa K, Kawaji N, Nagase D, Tatemoto H, Tsuzuki Y

  11th International Symposium on Spermatology  2010.07  -  2010.07 

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• Grant-in-Aid for Scientific Research(C)

  Project Year: 2021.04  -  2025.03 

  Direct: 3,100,000 (YEN)  Overheads: 930,000 (YEN)  Total: 4,030,000 (YEN)

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2016.04  -  2020.03 

  Direct: 3,700,000 (YEN)  Overheads: 1,110,000 (YEN)  Total: 4,810,000 (YEN)

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2016.04  -  2020.03 

  Direct: 3,700,000 (YEN)  Overheads: 4,810,000 (YEN)  Total: 1,110,000 (YEN)

• Study on the regulation of sulfated zona glycoproteins associated with the reduction of polyspermy during in vitro fertilization of porcine oocytes

  Grant-in-Aid for Scientific Research(C)

  Project Year: 2016.04  -  2020.03 

  Investigator(s): TATEMOTO Hideki 

  Direct: 3,700,000 (YEN)  Overheads: 4,810,000 (YEN)  Total: 1,110,000 (YEN)

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  This study was conducted to elucidate the reduction of polyspermy during IVF without adverse effect on embryo developmental competence through the regulation of sulfated glycoprotein of porcine zona pellucida (ZP). Treatment of oocytes with 5 mU/mL sulfatase for 10 min before the onset of insemination or 5 ng/ml ARSA blocking peptide during IVF effectively protected polyspermic fertilization under conditions that maintained the high sperm penetration rate and developmental potency. The number of sperm bound to ZP and the incidence of inducing acrosome reaction were significantly decreased by these treatments; however, it should be noted that the treatments had no effect against the time course of changes in ZP glycoproteins with egg activation. These findings indicate the possibility of reduction of polyspermy during porcine IVF but not the decrease of development to blastocyst stage in keeping with a role for sulfated glycoprotein of ZP and ARSA of spermatozoa.

• Study on the regulation of sulfated zona glycoproteins associated with the reduction of polyspermy during in vitro fertilization of porcine oocytes

  Grant-in-Aid for Scientific Research(C)

  Project Year: 2016.04  -  2020.03 

  Investigator(s): TATEMOTO Hideki 

  Direct: 3,700,000 (YEN)  Overheads: 4,810,000 (YEN)  Total: 1,110,000 (YEN)

  　View Summary

  This study was conducted to elucidate the reduction of polyspermy during IVF without adverse effect on embryo developmental competence through the regulation of sulfated glycoprotein of porcine zona pellucida (ZP). Treatment of oocytes with 5 mU/mL sulfatase for 10 min before the onset of insemination or 5 ng/ml ARSA blocking peptide during IVF effectively protected polyspermic fertilization under conditions that maintained the high sperm penetration rate and developmental potency. The number of sperm bound to ZP and the incidence of inducing acrosome reaction were significantly decreased by these treatments; however, it should be noted that the treatments had no effect against the time course of changes in ZP glycoproteins with egg activation. These findings indicate the possibility of reduction of polyspermy during porcine IVF but not the decrease of development to blastocyst stage in keeping with a role for sulfated glycoprotein of ZP and ARSA of spermatozoa.

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Preferred joint research theme 【 display / non-display 】

Preferred joint research theme 【 display / non-display 】

• The study on early development in the mammalian germ cells

  Animal Reproduction

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• 2021.04

  　

  　

  

• 2021.04

  　

  　

  

• 2019.04