Current Affiliation Organization 【 display / non-display 】

Current Affiliation Organization 【 display / non-display 】

University 【 display / non-display 】

University 【 display / non-display 】

Graduate School 【 display / non-display 】

Graduate School 【 display / non-display 】

External Career 【 display / non-display 】

External Career 【 display / non-display 】

display all >>

Affiliated academic organizations 【 display / non-display 】

Affiliated academic organizations 【 display / non-display 】

Research Areas 【 display / non-display 】

Research Areas 【 display / non-display 】

Published Papers 【 display / non-display 】

Published Papers 【 display / non-display 】

• Shape of scaffold controlling the direction of cell migration

  Sunami, H; Shimizu, Y; Kishimoto, H

  BIOPHYSICS AND PHYSICOBIOLOGY ( 一般社団法人 日本生物物理学会 )  21 ( 1 ) e210004   2024

  Type of publication: Research paper (scientific journal)

  　View Summary

  <p>Cell migration plays an important role in the development and maintenance of multicellular organisms. Factors that induce cell migration and mechanisms controlling their expression are important for determining the mechanisms of factor-induced cell migration. Despite progress in the study of factor-induced cytotaxis, including chemotaxis and haptotaxis, precise control of the direction of cell migration over a wide area has not yet been achieved. Success in this area would update the cell migration assays, superior cell separation technologies, and artificial organs with high biocompatibility. The present study therefore sought to control the direction of cell migration over a wide area by adjusting the three-dimensional shape of the cell scaffold. The direction of cell migration was influenced by the shape of the cell scaffold, thereby optimizing cell adhesion and protrusion. Anisotropic arrangement of these three-dimensional shapes into a periodic structure induced unidirectional cell migration. Three factors were required for unidirectional cell migration: 1) the sizes of the anisotropic periodic structures had to be equal to or lower than the size of the spreading cells, 2) cell migration was restricted to a runway approximately the width of the cell, and 3) cells had to be prone to extension of long protrusions in one direction. Because the first two factors had been identified previously in studies of cell migration in one direction using two-dimensional shaped patterns, these three factors are likely important for the mechanism by which cell scaffold shapes regulate cell migration.</p>

  • Access this article

    DOI
  • Search related information
    PubMed CiNii Research

• An alpaca single-domain antibody (VHH) phage display library constructed by CDR shuffling provided high-affinity VHHs against desired protein antigens.

  Tsukahara N, Murakami A, Motohashi M, Nakayama H, Kondo Y, Ito Y, Azuma T, Kishimoto H

  International immunology ( International Immunology )  34 ( 8 ) 421 - 434   2022.07 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

  • Access this article

    DOI
  • Search related information
    PubMed

• A 3D Microfabricated Scaffold System for Unidirectional Cell Migration.

  Sunami H, Shimizu Y, Denda J, Yokota I, Kishimoto H, Igarashi Y

  Advanced biosystems ( Advanced Biosystems )  4 ( 10 ) e2000113   2020.09 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

  • Access this article

    DOI
  • Search related information
    PubMed

• Adipose tissue-derived mesenchymal stem cells ameliorate bone marrow aplasia related with graft-versus-host disease in experimental murine models.

  Nishi Y, Murakami A, Murayama Y, Tsukahara N, Okamoto S, Nakachi S, Morichika K, Tamaki K, Noguchi H, Matsushita M, Karube KN, Fukushima T, Morishima S, Kishimoto H, Masuzaki H

  Transplant immunology ( Transplant Immunology )  55   101205   2019.08

  Type of publication: Research paper (scientific journal)

  • Access this article

    DOI
  • Search related information
    PubMed

• High-affinity IgM⁺ memory B cells are defective in differentiation into IgM antibody-secreting cells by re-stimulation with a T cell-dependent antigen.

  Tashiro Y, Murakami A, Hara Y, Shimizu T, Kubo M, Goitsuka R, Kishimoto H, Azuma T

  Scientific reports ( Scientific Reports )  8 ( 1 ) 14559   2018.09

  Type of publication: Research paper (scientific journal)

  • Access this article

    DOI
  • Search related information
    PubMed

display all >>

Other Papers 【 display / non-display 】

Other Papers 【 display / non-display 】

• [第2の生体の窓~21世紀のバイオイメージングの窓~]SBWイメージングの現状と課題

  曽我公平, 竹内司, 横田秀夫, 岸本英博

  バイオイメージング   24 ( 2 ) 60   2015.09

   

  J-GLOBAL

Grant-in-Aid for Scientific Research 【 display / non-display 】

Grant-in-Aid for Scientific Research 【 display / non-display 】

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2018.04  -  2021.03 

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2014.04  -  2017.03 

  Direct: 3,800,000 (YEN)  Overheads: 1,140,000 (YEN)  Total: 4,940,000 (YEN)