論文 - カーン　モハマドミザヌルラヒム

5種の野生ソラヌム属由来の細胞質を用いた、ナス（Solanum melongena L.）の細胞質雄性不稔（CMS）系統における稔性形質の比較研究析

Shiro Isshiki, Takashi Arita, Masaki Iwayoshi, Kenji Ureshino, Md. Mizanur Rahim Khan

New Zealand Journal of Crop and Horticultural Science   54 ( 1 )   2026年03月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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種無しピーマン品種の稔性形質の解析

カーン モハマド ミザヌル ラヒム, 芳賀 智仁, 岩吉 真輝, 有田 隆史, 渡辺 久修, 一色 司郎

植物環境工学 ( 日本生物環境工学会 )  37 ( 4 ) 103 - 109   2025年12月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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キンモウツツジ(Rhododendron oldhamii Maxim.)と日本に自生する常緑性ツツジ種との交配における秋咲き性の遺伝

嬉野 健次, 山城 秀人, モハマド ミザヌルラヒムカーン

園芸学研究 ( 一般財団法人園芸学会 )  24 ( 1 ) 21 - 27   2025年01月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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温帯および亜熱帯地域におけるSolanum kurziiおよびS. violaceum細胞質をもつナス雄性不稔系統の種子生産

モハマド ミザヌル ラヒム カーン・有田隆史・岩吉真輝・辻田有紀・一色司郎

農業生産技術管理学会誌 ( 農業生産技術管理学会 )  30 ( 2 ) 77 - 80   2023年12月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

Development of a male-sterile line of eggplant utilizing the cytoplasm of Solanum aethiopicum Gilo group

Khan, M. M. R., T. Arita, M. Iwayoshi, Y. Ogura-Tsujita, and S. Isshiki

Environmental Control in Biology ( Japanese Society of Agricultural, Biological and Environmental Engineers and Scientists )  61 ( 4 ) 63 - 67   2023年10月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Production of doubled haploid through anther culture of the male fertile lines in the CMS system of eggplant with the cytoplasm of Solanum grandifolium

Khan, M. M. R., T. Matsuyama, I. Nakamura, K. Ureshino, T. Arita, M. Iwayoshi, Y. Ogura-Tsujita and S. Isshiki

Environmental Control in Biology ( Japanese Society of Agricultural, Biological and Environmental Engineers and Scientists )  60 ( 3 ) 187 - 190   2022年07月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Pollen fertility differences in the progenies obtained from a cross between eggplant (Solanum melongena L.) as a seed parent and eggplant cytoplasmic substitution lines as pollen parents

Isshiki, S., I. Nakamura, K. Ureshino, M. M. R. Khan

Australian Journal of Crop Science ( SOUTHERN CROSS PUBLISHING-AUSTRALIA )  15 ( 2 ) 233 - 237   2021年02月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

To the best of our knowledge, there is no report about pollen fertility of the progenies developed using eggplant (Solanum melongena L.) as a seed parent and eggplant cytoplasmic substitution lines as pollen parents. Pollen fertility of these progenies is very important to use as restorer line in the eggplant’s hybrid breeding program. In this study, pollen fertility was investigated for the progenies which were produced using S. melongena ‘Uttara’ as a seed parent and the eggplant cytoplasmic substitution lines as pollen parents. To assess pollen fertility, pollen stainability and in vitro germination ability were investigated. Although the nuclear and the cytoplasmic genome of the progenies were almost identical to eggplant ‘Uttara’, a clear difference was observed in the pollen fertility due to the difference in the pollen parents having different wild Solanum cytoplasms. The progenies produced using the functional cytoplasmic male sterile (CMS) lines as a pollen parent, whose cytoplasm donor were S. kurzii, S. violaceum and S. virginianum, showed pollen release type and high pollen fertility almost equal to eggplant ‘Uttara’. It is considered that the characteristics of these progenies were almost the same as eggplant. On the other hand, the progenies that produced using the fertility restored lines of the pollen non-formation type CMS lines as a pollen parent, whose cytoplasm donors were S. aethiopicum, S. anguivi and S. grandifolium, showed pollen release type and low pollen fertility, i.e., pollen staining ability was about 54% and pollen germination ability were about 35%. It is considered that the cause of this low pollen fertility was the incompatibility between the eggplant cytoplasm and the eggplant nuclear genome, which seems to be modified in the process of continuous backcrossing under the wild Solanum cytoplasms. It is suggested that complete nuclear substitution is difficult by continuous backcrossing with eggplant in the alloplasmic lines with S. aethiopicum, S. anguivi and S. grandifolium cytoplasm donors. Incompatibility between the normal eggplant cytoplasm and the modified eggplant nuclear genomes of the alloplasmic lines with S. aethiopicum, S. anguivi and S. grandifolium cytoplasms might be a cause for the low pollen fertility of the investigated progenies

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Development of the Functional Male Sterile Line of Eggplant Utilizing the Cytoplasm of Solanum kurzii by Way of the Amphidiploid

Md Mizanur Rahim KHAN, Takashi ARITA, Masaki IWAYOSHI, Yuki OGURA-TSUJITA, Shiro ISSHIKI

Environmental Control in Biology ( Japanese Society of Agricultural, Biological and Environmental Engineers and Scientists )  58 ( 3 ) 79 - 83   2020年07月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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ネニラ（Allium hookeri Thwaites）とわが国のネギ属野菜の類縁関係

Md. Mizanur Rahim KHAN・岩吉真輝・有田隆史・一色司郎

熱帯農業研究 ( 日本熱帯農業学会 )  13 ( 1 ) 27 - 29   2020年06月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

Paternal inheritance of mitochondrial DNA of Cucumis hystrix in an interspecific cross between C. sativus and C. hystrix

Khan, M. M. R., M. Iwayoshi, T. Arita, S. Isshiki

Environmental Control in Biology ( Japanese society of agricultural, biological and environmental engineers and scientists )  57 ( 4 ) 119 - 121   2019年10月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Genetic characterization of the fertility restorer (Rf) genes and their linked DNA marker of the male sterile eggplant systems having the cytoplasm of the wild species

Khan, M. M. R., M. Iwayoshi, Y. Ogura-Tsujita, S. Isshiki

Environmental Control in Biology ( Japanese society of agricultural, biological and environmental engineers and scientists )  56 ( 4 ) 173 - 176   2018年10月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Pollen and seed fertility differences of the backcross progenies between Solanum virginianum and eggplant with different inheritance pattern of chloroplast DNA

Md Mizanur Rahim Khan, Mst Hasnunnahar, Masaki Iwayoshi, Yuki Ogura-Tsujita, Shiro Isshiki

SCIENTIA HORTICULTURAE ( ELSEVIER SCIENCE BV )  218   193 - 197   2017年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

To find out the relationship of fertility with chloroplast inheritance, pollen and seed fertility differences of the backcross progenies between Solanum virginianum and eggplant with different inheritance pattern of chloroplast DNA were investigated. In our previous study, we developed anther indehiscent male sterile line of eggplant by producing an interspecific F-1 hybrid (S. virginianum x S. melongena 'Senryo Nigou') followed by repeated backcrossing, using wild Solanum species S. virginianum as female parent and eggplant 'Uttara' as recurrent pollen parent. The BC1 and its succeeding generations were named as line A that carried recombinant chloroplast DNA (cpDNA) and showed anther indehiscent type of functional male sterility. In the present study, with the intention to make the BC1 progenies with maternal cpDNA, we tried to backcross the same F-1 hybrid to eggplant 'Uttara' again. The new BC1 and the succeeding backcross progenies named as line B that carried maternal cpDNA and showed anther dehiscence. The F-1 hybrid showed very low pollen and seed fertility. Pollen stainability and germination ability of line A were higher than those of the line B. Seed fertility of line B was found very low. On the contrary, seed fertility of line A was found high which was almost similar as eggplant 'Uttara'. The present study suggests that anther dehiscence, pollen and seed fertility differences between the backcross progenies of line A and line B controlled to some extent by chloroplast inheritance. (C) 2017 Elsevier B.V. All rights reserved.

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Pollen degeneration in three functional male-sterile lines of eggplant with the wild Solanum cytoplasms

Md Mizanur Rahim Khan, Mst Hasnunnahar, Masaki Iwayoshi, Yuki Ogura-Tsujita, Shiro Isshiki

HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY ( KOREAN SOC HORTICULTURAL SCIENCE )  56 ( 3 ) 350 - 357   2015年07月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

Pollen developing process was studied to identify the stages of pollen degeneration and to elucidate the factors controlling low pollen fertility in three functional cytoplasmic male-sterile (CMS) lines of eggplant 'Uttara'. The CMS lines of eggplant were developed by repeated backcrossing using the cytoplasms of wild Solanum species S. kurzii Brace & Prain, S. violaceum Ort., and S. virginianum L.. Anthers were squashed in 1% aceto-carmine to assess pollen staining ability and pollen degeneration at different stages of development. Unicellular microspores were released from tetrads after normal meiotic division. Pollen degeneration occurred at different stages of pollen development in CMS lines such as at unicellular microspore (29.3-36.3%), early bicellular pollen (5.5-12.2%), and late bicellular pollen (9.3-10.2%) stages. On the other hand, in eggplant, only 3.8% pollen was degenerated at unicellular microspore stage and there was negligible pollen degeneration at other stages. Among the stained pollen, abnormally stained (partly and faintly) pollen were found significantly higher in the CMS lines as compared to eggplant. Well stained pollen was varied from 23.2-31.9% in the CMS lines which was significantly lower than that of eggplant. Number of pollens per anther of CMS lines did not vary significantly from eggplant, except the CMS line with S. virginianum cytoplasm. In vitro pollen germination rate in the CMS lines was found to be significantly lower than that of eggplant. Starch accumulation and hydrolysis during pollen maturation were found incomplete in the CMS lines. Degeneration of pollen in different stages, abnormally stained pollen, incomplete starch accumulation and hydrolysis were most likely causes for low pollen fertility in these three CMS lines of eggplant.

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Pollen degeneration in three functional male-strile lines of eggplant with the wild Solanum cytoplasms

M. M. R. Khan, M. Hasnunnahar, M., Iwayoshi, Y. Ogura-Tsujita

Horticulture, Environment, and Biotechnology ( Korean society for horticultural science )  56 ( 3 ) 350 - 357   2015年06月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Fertility restoration in three CMS systems of eggplant by the Rf genes of each other’s systems and their SCAR marker

M. M, R. Khan, M. Hasnunnahar, M, Iwayoshi, S. Isshiki

Scientia Horticulturae ( ELSEVIER SCIENCE BV )  172   149 - 154   2014年06月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

Three pollen non-formation type cytoplasmic male sterility (CMS) systems of eggplant (Solanum melongena L.) were developed in our previous study, through repeated backcrossing, by using wild Solanum species Solanum aethiopicum L Aculeatum Group, Solanum anguivi Lam. and Solon urn grandifolium C. V. Morton as the cytoplasm donor and eggplant 'Uttara' as the recurrent pollen parent. Each CMS system has male fertile (MF) line which carries the fertility restorer (Rf) genes for the pollen non-formation type sterility. In the present study, fertility restoration ability in three CMS systems of eggplant by the Rf genes of each other's systems was evaluated. The male sterile (MS) line of each CMS system was pollinated with the pollen of MF lines of other CMS systems as well as its own CMS system. Among the nine cross combinations, six groups were obtained from crossing MS lines with MF lines of other CMS systems and three groups were obtained from crossing MS lines with MF lines of their own CMS system. The segregation of MF and MS plants was fitted well with a 3:1 ratio in almost all cross combinations. The segregation patterns revealed that two independent dominant Rf genes restore fertility in each CMS system. Pollen stainability and in vitro pollen germination rate of the progenies obtained from different cross combinations were varied from 61.0 to 89.8% and 0.7 to 8.0%, respectively. Each Rf gene of three CMS systems restores fertility of other CMS systems as well as its own CMS system. Moreover, fertility recovery action in these three kinds of CMS is similar. DNA markers linked to Rf genes were screened by bulked segregant analysis (BSA) using random amplified polymorphic DNA (RAPD). A total of 420 primers were used, and more than 65% of the primers yielded a considerable number of RAPD fragments in both MF and MS bulks. Only one Operon primer OPAB10 gave a reproducible polymorphic band of 1.9 kbp (OPAB10(1900)) between bulks and was identified as linked to the Rf genes.Additionally, the RAPD marker OPAB10(1900) was converted to sequenced characterized amplified region (SCAR) marker designated as SCAB10(1900) by nucleotide sequencing. Findings of the present study may give us a potential way to explore CMS and the corresponding Rf genes from wild Solanum resources for the breeding program of eggplant. (C) 2014 Elsevier B.V. All rights reserved.

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Pollen and seed fertility of the male fertile lines having the fertility restorer gene in three CMS systems of eggplant

Md. Mizanur Rahim Khan, Mst. Hasnunnahar, Masaki Iwayoshi, Shiro Isshiki

Scientia Horticulturae   157   39 - 44   2013年06月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

In our previous study, three pollen non-formation type cytoplasmic male-sterility (CMS) systems of eggplant (Solanum melongena L.) were developed through repeated backcrossing, by using wild Solanum species S. aethiopicum L. Aculeatum Group, S. anguivi and S. grandifolium as the cytoplasm donor and eggplant 'Uttara' as the recurrent pollen parent. Each CMS system has male fertile line which carries the fertility restorer genes for the pollen non-formation type sterility. In the present study, pollen and seed fertility of the male fertile lines were studied to find out the possibilities of selfing and anther culture for the development of homozygous restorer and male-sterile lines. To confirm the utility of these methods we tried to apply the method of selfing and anther culture of the male fertile lines. Pollen fertility was assessed by pollen stainability in acetocarmine, in Lugol's solution and in vitro germination rate of pollen at three different stages of pollen maturation, such as, 2 days before anthesis, at anthesis and 2 days after anthesis. Pollen stainability with acetocarmine and in vitro germination rate of pollen in all three stages of pollen maturation were comparatively lower in the male fertile lines than eggplant 'Uttara'. At anthesis, pollen stainability was about 50% and in vitro pollen germination was less than 20% in three male fertile lines. Pollen stainability with Lugol's solution revealed that the low pollen fertility of the male fertile lines would be caused by incomplete accumulation and degradation of starch during pollen maturation. The male fertile lines showed good fruit set percentage and produced an adequate amount of seeds in selfing and backcrossing. A restorer line homozygous for the fertility restorer gene with the cytoplasm of S. anguivi was developed by selfing and selection of the male fertile line. A doubled haploid male sterile and male fertile plants were obtained from anther culture of the male fertile lines with the cytoplasms of S. anguivi and S. grandifolium, respectively. Pollen and seed fertility study of the present study revealed that homozygous restorer and male-sterile lines could be developed by selfing and anther culture of the male fertile lines of eggplant. © 2013 Elsevier B.V.

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Production of Amphidiploids of the Hybrids between Solanum macrocarpon and Eggplant

Md. Mizanur Rahim Khan, Mst. Hasnunnahar, S. Isshiki

HORTSCIENCE ( AMER SOC HORTICULTURAL SCIENCE )  48 ( 4 ) 422 - 424   2013年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

To restore fertility of the F-1 between Solanum macrocarpon and eggplant, amphidiploids were produced through doubling of chromosomes by colchicine treatment. Shoot tips and axillary buds of F-1 plants were kept for 2 and 4 days in liquid Murashige and Skoog (MS) media with 0.05% colchicine. As a result of colchicine treatment, two amphidiploids were identified by observing root tip cell chromosome number, stomatal guard cell size, and pollen characteristics. The amphidiploids contained 48 chromosomes, twice the normal diploid number of 24. Stomata size and pollen diameter were significantly larger in amphidiploids than F-1. Flower diameter and length and width of anther, petal, and sepal were significantly larger in the amphidiploids than in F-1. Pollen stainability was 40% in amphidiploids but only 0.86% in the diploid F-1. The amphidiploids, after selfing and backcrossing with S. macrocarpon, set fruits with healthy seeds. Therefore, production of amphidiploids by colchicine treatment restored the pollen and seed fertility of F-1 between S. macrocarpon and eggplant. The amphidiploids produced in this study would be very useful in future breeding programs of eggplant.

Pollen and seed fertility of three functional male-sterile lines of eggplant with the wild Solanum cytoplasms

Mst Hasnunnahar, Md. Mizanur, Rahim Khan, Shiro Isshiki

SCIENTIA HORTICULTURAE ( ELSEVIER SCIENCE BV )  139   58 - 61   2012年05月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

To find out the possibilities of propagation by selling and production of pure lines through anther culture, pollen fertility and seed fertility were investigated in three functional male-sterile lines of eggplant developed by continuous backcross method using wild Solanum species Solanum kurzii, Solanum violaceum and Solanum Virginian urn as a cytoplasm donor and Solanum melongena 'Uttara' as a nucleus donor. Pollen fertility was assessed by pollen stainability in acetocarmine, in Lugol's solution and in vitro germination rate of pollen at three different stages of pollen maturation, such as, 2 days before anthesis, at anthesis and 2 days after anthesis. Pollen stainability with acetocarmine and in vitro germination rate of pollen in all three stages of pollen maturation were comparatively lower in the male-sterile lines. Pollen stainability. with Lugol's solution revealed that, the low pollen fertility of the male-sterile lines would be caused by incomplete degradation of starch during pollen maturation. The male-sterile lines showed high percentage of fruit set with large number of seeds in the fruits obtained from selling. Our results confirm that, it is possible to propagate the male-sterile lines by selling and to produce pure male-sterile lines through anther culture. (C) 2012 Elsevier B.V. All rights reserved.

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Inheritance analysis of fertility restoration genes (Rf) in a male sterile system of eggplant using cytoplasm of Solanum grandifolium

M. Hasnunnahar, M. M. R. Khan, S. Isshiki

Australian Journal of Crop Science   6   475 - 479   2012年03月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

Development of a cytoplasmic male-sterile line of eggplant (Solanum melongena L.) with the cytoplasm of Solanum anguivi

M. M. R. Khan, S. Isshiki

PLANT BREEDING ( WILEY )  130 ( 2 ) 256 - 260   2011年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

P>To develop a new cytoplasmic male-sterile line of eggplant utilizing the Solanum anguivi cytoplasm, the interspecific hybrid (S. anguivi x eggplant 'Senryo 2 gou') was repeatedly backcrossed to eggplant 'Uttara' up to BC5 generation to achieve cytoplasm substitution. Chloroplast and mitochondrial DNA analyses confirmed that backcross progenies had the S. anguivi cytoplasm. In the backcross progenies, male-sterile plants, whose anthers were completely devoid of pollen grains, appeared. All the backcross generations obtained from the male-fertile seed parents segregated into male-fertile and sterile types with a 3 : 1 ratio, whereas those obtained from the male-sterile one were all male-sterile. The selfed progeny from a male-fertile BC5 plant (selfed progeny 1) and the selfed progeny from the male-fertile selfed progeny 1 segregated at 15 : 1 and 3 : 1 ratios, respectively. These results indicated that two independent dominant fertility restorer (Rf) genes control pollen formation. A new male-sterile line of eggplant could be developed by utilizing the cytoplasm of S. anguivi.

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