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• A case of oral paracoccidioidomycosis difficult to differentiate from oral carcinoma

  Kentaro Ide, Sho Miyamoto, Kenji Chibana, Tomoko Tamaki, Makoto Murahashi, Nobuyuki Maruyama, Jumpei Shirakawa, Takahiro Goto, Naoki Wada, Toshihiro Kawano

  Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology   35 ( 3 ) 272 - 276   2023.05 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  Paracoccidioidomycosis (PCM) is a fungal disease endemic to Latin America. PCM is a systemic granulomatous disease that primarily affects the lungs and causes ulcerative and granulomatous lesions in the oral cavity. Diagnosis is often difficult owing to the rarity of the disease outside of Latin America. Therefore, we present a rare case of oral PCM that is difficult to differentiate from oral carcinoma. The patient was a 76-year-old Japanese man who had previously worked in Bolivia decades ago. A 14 × 18 mm intractable ulcer under a fixed partial prosthesis in the mandibular posterior region caused severe contact pain. Oral carcinoma was initially suspected, and a detailed examination was performed. However, the ulcer was diagnosed as PCM on biopsy. The oral lesion improved after an antifungal drug was administered. At approximately seven months follow-up, the patient remained recurrence-free.

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• A Comprehensive Study of the Immunophenotype and its Clinicopathologic Significance in Adult T-Cell Leukemia/Lymphoma.

  Tomoko Tamaki, Kennosuke Karube, Shugo Sakihama, Yuma Tsuruta, Ryoko Awazawa, Masaki Hayashi, Norihiro Nakada, Hirofumi Matsumoto, Nobutake Yagi, Kazuiku Ohshiro, Iwao Nakazato, Sakiko Kitamura, Yukiko Nishi, Takuya Miyagi, Sayaka Yamaguchi, Sawako Nakachi, Satoko Morishima, Hiroaki Masuzaki, Kenzo Takahashi, Takuya Fukushima, Naoki Wada

  Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc   36 ( 8 ) 100169 - 100169   2023.03 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  Adult T-cell leukemia/lymphoma (ATLL) is a mature T-cell tumor caused by human T-lymphotropic virus type 1 (HTLV-1). The typical ATLL immunophenotypes are described in the 2017 World Health Organization Classification of Tumours of Haematopoietic and Lymphoid Tissues (positive: CD2, CD3, CD5, CD4, and CD25; negative: CD7, CD8, and cytotoxic markers; and partially positive: CD30, CCR4, and FOXP3). However, limited studies are available on the expression of these markers, and their mutual relationship remains unknown. Furthermore, the expression status of novel markers associated with T-cell lymphomas, including Th1 markers (T-bet and CXCR3), Th2 markers (GATA3 and CCR4), T follicular helper markers (BCL6, PD1, and ICOS), and T-cell receptor (TCR) markers, and their clinicopathologic significance is unclear. In this study, we performed >20 immunohistochemical stains in 117 ATLL cases to determine the comprehensive immunophenotypic profile of ATLL, which were compared on the basis of clinicopathologic factors, including morphologic variants (pleomorphic vs anaplastic), biopsy locations, treatments, Shimoyama classification-based clinical subtype, and overall survival. CD3+/CD4+/CD25+/CCR4+ was considered a typical immunophenotype of ATLL, but approximately 20% of cases did not conform to this pattern. Simultaneously, the following new findings were obtained: (1) most cases were negative for TCR-β and TCR-δ (104 cases, 88.9%), indicating the usefulness of negative conversion of TCR expression to provide differentiation from other T-cell tumors; (2) the positivity of CD30 and CD15 and the negativity of FOXP3 and CD3 were significantly associated with anaplastic morphology; and (3) atypical cases, such as T follicular helper marker-positive (12 cases, 10.3%) and cytotoxic molecule-positive cases (3 cases, 2.6%), were identified. No single markers could predict the overall survival among patients with acute/lymphoma subtypes of ATLL. The results of this study illustrate the diversity of ATLL phenotypes. In T-cell tumors occurring in HTLV-1 carriers, the possibility of ATLL should not be eliminated even when the tumor exhibits an atypical phenotype, and the confirmation of HTLV-1 in the tissue is recommended.

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• Reconstruction of the lateral collateral ligament using the plantaris tendon after wide excision of soft tissue sarcoma of the knee: A case report

  Yuichi Tsuha, Hiromichi Oshiro, Kohei Mizuta, Tomoko Tamaki, Yasunori Tome, Naoki Wada, Kotaro Nishida

  Molecular and Clinical Oncology ( Spandidos Publications )  18 ( 3 )   2023.02 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Juxtaglomerular cell tumor with pulmonary metastases: A case report and review of the literature.

  Hideki Sakiyama, Satoru Hamada, Tokiko Oshiro, Nobuyuki Hyakuna, Masaaki Kuda, Tomoro Hishiki, Hajime Aoyama, Naoto Kuroda, Kenji Yorita, Naoki Wada, Takako Yoshioka, Yuhki Koga, Koichi Nakanishi

  Pediatric blood & cancer     e30068   2022.12 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Intraductal carcinoma of the parotid gland―A case report―

  HIRATA Yukiya, HIGUCHI Kayoko, NAGAO Toshitaka, ZUKERAN Yoko, KINJO Takao, WADA Naoki

  The Journal of the Japanese Society of Clinical Cytology ( The Japanese Society of Clinical Cytology )  61 ( 6 ) 431 - 437   2022.11 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  <i><b>Background</b></i> : Intraductal carcinoma (IDC) is a rare salivary gland tumor with a good prognosis. <i><b>Case</b></i> : The patient was a man in his seventies who presented to us with a 10-year history of a small left parotid mass that had started to increase in size approximately one year ago. MRI revealed a 50-mm multilocular cystic mass caudally over the inferior pole of the left parotid gland, and fine-needle aspiration cytology (FNAC) was performed. The FNAC specimen revealed a mildly overlapping epithelial mass in a background of lymphocytes, neutrophils, and macrophages phagocytosing hemosiderin. The nuclei of the tumor cells were relatively small and mildly atypical, with granular chromatin and one or two small nucleoli. The tumor cells contained greenish and vacuolated cytoplasm and intracytoplasmic lumina containing secretions as well as brownish-red granules. Since the cytological findings suggested a low-grade malignant lesion, we categorized the findings as “suspicion for malignancy, low grade" according to The Milan System for Reporting Salivary Gland Cytopathology, with the differential diagnosis including secretory carcinoma and low-grade adenocarcinoma (cystadenocarcinoma). <i><b>Conclusion</b></i> : IDC may be cytologically similar to secretory carcinoma and low-grade adenocarcinoma (cystadenocarcinoma), which may pose difficulties in accurate estimation of the histological type. However, since IDC has a more favorable prognosis than other malignant salivary gland tumors, it is important to confirm the low-grade malignant nature of the tumor by FNA before surgery.

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• SDPRは子宮体部類内膜癌においてILKを介してALDH1を制御する

  田原 紳一郎, 野島 聡, 大島 健司, 堀 由美子, 倉重 真沙子, 和田 直樹, 本山 雄一, 奥崎 大介, 池田 純一郎, 森井 英一

  日本癌学会総会記事 ( (一社)日本癌学会 )  79回   YSA - 2   2020.10

   

• 動脈炎におけるステロイド治療が及ぼす組織学的変化(Histological change of Arteritis under steroid treatment)

  田中 さやか, 佐々木 香織, 皆見 勇人, 野浦 郁恵, 小林 郁江, 大畑 麻衣, 和田 直樹, 桑江 優子, 大澤 政彦

  日本病理学会会誌 ( (一社)日本病理学会 )  109 ( 1 ) 459 - 459   2020.03

   

• 肝reactive lymphoid hyperplasiaの1例(A case of reactive lymphoid hyperplasia of the liver)

  野浦 郁恵, 佐々木 香織, 皆見 勇人, 小林 郁江, 大畑 麻衣, 和田 直樹, 田中 さやか, 桑江 優子, 大澤 政彦

  日本病理学会会誌 ( (一社)日本病理学会 )  109 ( 1 ) 350 - 350   2020.03

   

• SDPR Regulates ALDH1 via ILK Signaling in Endometrioid Carcinoma Cells

  田原紳一郎, 野島聡, 大島健司, 堀由美子, 倉重真沙子, 和田直樹, 本山雄一, 奥崎大介, 池田純一郎, 森井英一

  日本癌学会学術総会抄録集(Web)   79th   2020

   

  J-GLOBAL

• Serine racemaseはミトコンドリアの容量を維持することによって結腸癌細胞増殖を加速する(Serine racemase enhances colon cancer cell proliferation by maintaining mitochondrial mass)

  大島 健司, 野島 聡, 田原 紳一郎, 倉重 真沙子, 川崎 桂輔, 堀 由美子, 和田 直樹, 森井 英一

  日本病理学会会誌 ( (一社)日本病理学会 )  108 ( 1 ) 310 - 310   2019.04

   

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Grant-in-Aid for Scientific Research 【 display / non-display 】

Grant-in-Aid for Scientific Research 【 display / non-display 】

• Grant-in-Aid for Scientific Research(C)

  Project Year: 2018.04  -  2021.03 

  Direct: 3,500,000 (YEN)  Overheads: 1,050,000 (YEN)  Total: 4,550,000 (YEN)

• The positive marker identification of tumor-initiating cells and the dynamic analyses of them in lymphoplasmacytic lymphoma (LPL)

  Grant-in-Aid for Scientific Research(C)

  Project Year: 2015.04  -  2018.03 

  Investigator(s): WADA Naoki 

  Direct: 3,800,000 (YEN)  Overheads: 1,140,000 (YEN)  Total: 4,940,000 (YEN)

  　View Summary

  MWCL-1 was established as LPL cell line. MWCL-1 cells were classified into three subpopulations by flow cytometric analyses using anti-CD20 and anti-CD138 antibodies: CD20-CD138-, CD20+CD138-, and CD20+CD138+ cells. We searched for markers preferentially expressed in CD20-CD138- cells of MWCL-1 (the candidates for tumor-initiating cells of LPL), and the chemokine receptor CXCR7 was isolated. We also searched for favorable microenvironments for their proliferation, and we found that hypoxia and CXCL12-CXCR7 signaling appeared to be favorable microenvironments. We then examined the effect of plasma-activated medium (PAM) on LPL cell line, and we found that PAM induced plasma cell (CD138 positive) differentiation, which appeared to be mediated by PRDM1α (the transcription factor promoting plasma cell differentiation).

• Dynamic analyses of tumor-initiating cells in lymphoplasmacytic lymphoma (LPL)

  Grant-in-Aid for Young Scientists(B)

  Project Year: 2013.04  -  2015.03 

  Investigator(s): WADA NAOKI 

  Direct: 3,200,000 (YEN)  Overheads: 960,000 (YEN)  Total: 4,160,000 (YEN)

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  MWCL-1 was established as LPL cell line. MWCL-1 cells were classified into three subpopulations using a flow cytometer with anti-CD20 and anti-CD138 antibodies: CD20-CD138-, CD20+CD138-, and CD20+CD138+. When cultured, CD20-CD138- cells yielded all three subpopulations. Compared to the other subpopulations, CD20-CD138- cells possessed the efficient ROS expelling and in vitro colony formation activities, and were resistant to apoptosis. These results suggested that CD20-CD138- cells might be a candidate for tumor-initiating cells in LPL. Low oxygen culture of MWCL-1 induced the production of CD20-CD138- cells, and this result suggested that hypoxia was an advantageous microenvironment for CD20-CD138- cells. CXCR7 mRNA/protein expression was the highest in CD20-CD138- cells. When CXCL12, a ligand of CXCR7, was added, the proportion of CD20-CD138- cells significantly increased. CXCL12-CXCR7 signaling might exert a great influence on CD20-CD138- cells.

• Investigation into the relationship between prognosis and characteristics of macrophages in malignant lymphomas

  Grant-in-Aid for Young Scientists(B)

  Project Year: 2011  -  2012 

  Investigator(s): WADA Naoki 

  Direct: 3,000,000 (YEN)  Overheads: 900,000 (YEN)  Total: 3,900,000 (YEN)

  　View Summary

  Immunohistochemical double staining with antibodies for HLA-DR/CD68 (markers for M1-tumour-associated macrophage [M1-TAM]) or CD163/CD68 (M2-TAM) was performed to investigate the significance of TAMs in diffuse large B-cell lymphoma (DLBCL) and Hodgkin lymphoma (HL). In DLBCL, a higher number of M2-TAM was a significant factor for poor prognosis. The overall survival rate of the patients with DLBCL-IF, DLBCL showing an interfollicular pattern of proliferation preserving lymphoid follicles, was significantly better than those withDLBCL control group. All DLBCL-IF patients with a low M2-TAM count were alive at the end of observation. In HL, a higher number of M2-TAM was significantly correlated with shorter overall survival. When confined to mixed cellularity subtype of HL, a higher number of M1-TAM was significantly correlated with favorable prognosis.