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• Adenosine Receptor Mechanisms Underlying Bladder Dysfunction in Male Rats With Bladder Outlet Obstruction

  Takaoka, EI; Kurobe, M; Matsuoka, K; Kamijo, T; Kimura, S; Watton, PN; Robertson, AM; Yoshimura, N

  NEUROUROLOGY AND URODYNAMICS ( Neurourology and Urodynamics )  44 ( 6 ) 1370 - 1377   2025.08 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  <h4>Aims</h4>We examined the role of subtypes of adenosine receptors in bladder dysfunction and changes in the adenosine receptor expression in the bladder using male rats with partial bladder outlet obstruction (BOO).<h4>Methods</h4>In Sprague-Dawley rats (male 8-weeks old), BOO was produced by a partial ligation of the urethra along a metal rod of a 1.2 mm outer diameter. Control rats underwent sham operation. Awake cystometrograms (CMG) were first recorded during saline instillation, and then an adenosine A1 receptor agonist (CCPA, 4.1 μM), an adenosine A2A antagonist (ZM241385, 15 μM), or inosine (1 mM) were applied intravesically in sham and BOO rats. In addition, mRNA levels of adenosine receptor subtypes in the bladder wall were measured using RT-PCR. Histological studies of bladder specimen were also performed.<h4>Results</h4>Weights of BOO bladders were significantly (p < 0.0001) larger compared with sham bladders. In CMG, a number of non-voiding contractions (NVCs), bladder contraction amplitudes during voiding, bladder capacity, and post-void residual (PVR) were significantly (p < 0.001) increased compared with sham rats. Voiding efficiency (VE) was significantly (p < 0.001) reduced in BOO versus sham rats. Intravesical application of CCPA or inosine did not induce statistically significant effects on CMG parameters in BOO rats. Yet, ZM241385 induced a significant (p = 0.040) reduction in NVCs of BOO rats. mRNA levels of adenosine A2A and A3 receptors were significantly (p < 0.0001 and p = 0.0145, respectively) upregulated in the BOO bladder mucosa, whereas adenosine A2B receptors showed a significant (p < 0.0001) reduction in the BOO bladder mucosa compared with sham bladders. Histologically, we found the thickened detrusor muscle layer in BOO versus sham rats.<h4>Conclusions</h4>The male rat model of BOO seems to be suitable for exploring urethral obstruction-related bladder dysfunction at the compensated phase. In addition, the adenosine A2A receptor subtype would be a potential target for the treatment of male BOO patients with bladder overactivity.<h4>Clinical trial registration</h4>A clinical trial registration is not required as this study reported the basic research data using animal models.

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• Time-dependent bladder activity changes in streptozotocin-induced female diabetic rats

  Izumi, K; Kamijo, TC; Oshiro, T; Kimura, R; Ashikari, A; Kurobe, M; Akimoto, T; Miyazato, M

  PHYSIOLOGICAL REPORTS ( Physiological Reports )  13 ( 4 ) e70220   2025.02 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Feasibility of high-frequency ultrasound for seminiferous tubule assessment and correlation of B-mode imaging with pathological findings in the testis in azoospermia

  Ohta, T; Kojo, K; Kurobe, M; Numahata, D; Tatsuya, T; Okada, S; Iwamoto, T

  JOURNAL OF MEDICAL ULTRASONICS ( Journal of Medical Ultrasonics )  51 ( 3 ) 465 - 475   2024.07 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  PURPOSE: To determine the feasibility of high-frequency ultrasound (HFUS) for assessing seminiferous tubules and to understand high-resolution B-mode images of the testes in cases of azoospermia. METHODS: We verified how the histopathological images of testicular biopsy specimens can be observed using HFUS images and measurement analysis of seminiferous tubules was performed to 28 testes of 14 cases with azoospermia who underwent preoperative ultrasound and microdissection testicular sperm extraction (micro-TESE). The population consisted of obstructive azoospermia (OA) and non-obstructive azoospermia (NOA), including Sertoli cell-only syndrome (SCOS), and the other pathologies. Statistical verification of differences in seminiferous tubule diameters among preoperative ultrasound examination, ultrasound examination of pathological specimens, and histopathological specimens. We also examined the imagingpathology correlation via a case series presentation, aiming to identify imaging markers of testicular pathology and determine the possibility of predicting each condition. RESULTS: A comparison between HFUS images and histopathology from the same biopsy specimens suggested that ultrasonography could be seen as stereoscopic images due to its significantly greater slice thickness. The diameters of tubules were generally larger in pathological tissues as compared to ultrasonographic findings in OA and SCOS, but not in the other conditions. Comparisons provided insights into the predictability of SCOS and revealed imaging findings such as gaps between tubules and decreased diameter reflective of testicular damage. CONCLUSION: Seminiferous tubules can be observed however the diameter of seminiferous tubules varies in imaging and histopathology depending on the pathology. Imaging findings that reflect testicular damage and the predictability of SCOS were revealed in this study, but further verification is required.

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• 精細管の評価に高周波超音波画像診断による実行可能性と無精子症の精巣に対する超音波検査Bモードと病理所見との相関性(Feasibility of high-frequency ultrasound for seminiferous tubule assessment and correlation of B-mode imaging with pathological findings in the testis in azoospermia)

  Ohta Tomoyuki, Kojo Kosuke, Kurobe Masahiro, Numahata Daisuke, Takayama Tatsuya, Okada Shinya, Iwamoto Teruaki

  Journal of Medical Ultrasonics ( シュプリンガー・ジャパン(株) )  51 ( 3 ) 465 - 475   2024.07 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  無精子症患者の精細管の評価に、高周波超音波検査(HFUS)の実行可能性について検討した。2020年9月～2023年3月までの期間内に、著者らの所属する医療施設で、術前超音波検査と顕微鏡下精巣内精子採取術を受けた患者のうち、選択基準を満たした14例(年齢26～76歳)の28精巣を評価した。その結果、14例のうち閉塞性無精子症(OA)は4例で、セルトリ細胞遺残症候群(SCOS)は4例、Klinefelter症候群は2例、化学療法後は2例、他病型の精子低形成が2例に認められた。また、HFUSにより精細管が描出され、OAとSCOSの精細管直径は超音波画像よりも病理組織像で大きく観察され、HFUS所見と病理所見との比較により、SCOSの予測可能性に関する知見が得られ、精巣障害を反映する画像所見が得られた。これらの所見から、HFUSは精細管の評価に実行可能と結論付けられた。

• A Case of Proliferative Cystitis Discovered as Protruding Lesion during Inspection of Secondary Infertility

  Okamoto Keita, Kojo Kosuke, Kurobe Masahiro, Nakazato Yoshimasa, Inai Hiromu, Uchida Katsunori, Miyazaki Jun, Takayama Tatsuya

  Hinyokika kiyo. Acta urologica Japonica   70 ( 6 ) 167 - 171   2024.06 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  A 42-year-old man visited our hospital complaining of secondary infertility. An abdominal ultrasonography screening incidentally revealed a protruding lesion in the bladder. As the lesion extended from the prostatic urethra and bladder neck, there was a possibility of ejaculation dysfunction after resection of the lesion. Therefore, with the patient's informed consent, sperm cryopreservation was conducted for fertility preservation, and subsequently histological examination was performed by partial transurethral resection of bladder tumor. The pathological findings were proliferative cystitis including all three subtypes (glandularis, cystica, and papillary). Cyclooxygenase-2 immunostaining was positive in cytoplasm; weakly positive in cystic and papillary lesions, and strongly positive in glandular lesions. According to a literature review of massive proliferative cystitis, the patient was the 77th case in Japan. Novel postoperative immunological pharmacotherapies with cyclooxygenase-2 inhibitors have been introduced in recent years.

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• DIFFERENTIAL EFFECTS ON URETHRAL FUNCTION, EPITHELIAL MORPHOLOGY, AND MOLECULAR PROFILE IN TWO DIFFERENT RAT MODELS OF STRESS URINARY INCONTINENCE BY SIMULATED BIRTH TRAUMA OR ESTROGEN DEFICIENCY

  Kurobe, M; Takaoka, E; Suzuki, T; Mizoguchi, S; Saito, T; Alexandre, CE; Gotoh, D; Igarashi, T; Inamura, S; Ando, T; Inai, H; Uchida, K; Miyazaki, J; Yoshimura, N

  JOURNAL OF SEXUAL MEDICINE   19 ( 5 ) S135 - S135   2022.05  [Refereed]

   

• Progressive changes of bladder function, histology, and molecular expression in early and late-phases of partial bladder outlet obstruction in a male rat model(和訳中)

  黒部 匡広, 高岡 栄一郎, 齊藤 徹一, 後藤 大輔, 五十嵐 太郎, 宮崎 淳, 西山 博之, 吉村 直樹

  日本泌尿器科学会総会 ( (一社)日本泌尿器科学会総会事務局 )  109回   ISP03 - 04   2021.12  [Refereed]

   

• 雄ラット膀胱出口部部分閉塞の初期および後期における膀胱機能、組織像および分子発現の経時的変化(Progressive changes of bladder function, histology, and molecular expression in early and late-phases of partial bladder outlet obstruction in a male rat model)

  黒部 匡広, 高岡 栄一郎, 齊藤 徹一, 後藤 大輔, 五十嵐 太郎, 宮崎 淳, 西山 博之, 吉村 直樹

  日本泌尿器科学会総会 ( (一社)日本泌尿器科学会総会事務局 )  109回   ISP03 - 04   2021.12  [Refereed]

   

• CHARACTERIZATION OF FUNCTIONAL AND HISTOLOGICAL CHANGES OF THE URETHRA IN RAT MODELS OF STRESS URINARY INCONTINENCE (SUI) INDUCED BY SIMULATED BIRTH TRAUMA OR ESTROGEN DEFICIENCY

  Kurobe, M; Takaoka, E; Yoshikawa, S; Kadekawa, K; Kitta, T; Cao, N; Alexandre, E; Miyazaki, J; Nishiyama, H; Yoshimura, N

  NEUROUROLOGY AND URODYNAMICS   39   S180 - S182   2020.07  [Refereed]

   

• THERAPEUTIC EFFECTS OF INTRAVESICAL INSTILLATION OF LIPOSOME-CONJUGATED NGF ANTISENSE ON NGF OVEREXPRESSION IN THE BLADDER AND BLADDER OVERACTIVITY IN A RAT MODEL OF PROSTATIC INFLAMMATION

  Igarashi, T; Tyagi, P; Mizoguchi, S; Kurobe, M; Gotoh, D; Saito, T; Wang, Z; Furuta, A; Suzuki, Y; Egawa, S; Yoshimura, N

  NEUROUROLOGY AND URODYNAMICS   39   S238 - S239   2020.07  [Refereed]

   

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Presentations 【 display / non-display 】

Presentations 【 display / non-display 】

• 慢性陰嚢痛に対する顕微鏡下精索除神経術の治療成績についての検討(Therapeutic results of microsurgical denervation of spermatic cord for chronic testicular pain)

  Numahata Daisuke, Kojo Kosuke, Kurobe Masahiro, Ushijima Kei, Okamoto Keita, Inai Hiromu, Uchida Katsunori, Ohta Tomoyuki, Takayama Tatsuya

  日本泌尿器科学会総会  1900.01  -  1900.01 

• 尿膜管腫瘍が疑われた尿膜管嚢胞に対し、ロボット支援膀胱部分切除術を施行した1例(Robot-Assisted Partial Cystectomy for Urachal remnants, suspected of urachal tumor)

  Kurobe Masahiro, Ushijima Kei, Numahata Daisuke, Inai Hiromu, Uchida Katsunori, Kamada Teppei, Ohta Tomoyuki, Nakazato Yoshimasa, Takayama Tatsuya

  日本泌尿器科学会総会  1900.01  -  1900.01 

• 国際医療福祉大学病院におけるロボット支援下根治的前立腺全摘除術後のQOLと機能的転帰(Postoperative QOL and functional outcomes after Robot-Assisted Radical Prostatectomy in IUHW hospital)

  Okamoto Keita, Kurobe Masahiro, Takaoka Eiichiro, Inai Hiromu, Takayama Tatsuya, Uchida Katsunori, Miyazaki Jun

  日本泌尿器科学会総会  1900.01  -  1900.01 

• 雄ラット膀胱出口部部分閉塞の初期および後期における膀胱機能、組織像および分子発現の経時的変化(Progressive changes of bladder function, histology, and molecular expression in early and late-phases of partial bladder outlet obstruction in a male rat model)

  黒部 匡広, 高岡 栄一郎, 齊藤 徹一, 後藤 大輔, 五十嵐 太郎, 宮崎 淳, 西山 博之, 吉村 直樹

  日本泌尿器科学会総会  1900.01  -  1900.01 

• 精索静脈瘤手術の予後因子としての血中亜鉛濃度の検討(Analysis of serum zinc concentration as a prognostic factor in varicocelectomy)

  Kojo Kosuke, Tanaka Takazo, Kurobe Masahiro, Numahata Daisuke, Kimura Tomokazu, Nishiyama Hiroyuki, Ohta Tomoyuki, Takayama Tatsuya

  日本泌尿器科学会総会  1900.01  -  1900.01 

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