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Published Papers 【 display / non-display 】

• Time-dependent bladder activity changes in streptozotocin-induced female diabetic rats

  Izumi, K; Kamijo, TC; Oshiro, T; Kimura, R; Ashikari, A; Kurobe, M; Akimoto, T; Miyazato, M

  PHYSIOLOGICAL REPORTS ( Physiological Reports )  13 ( 4 ) e70220   2025.02 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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• Feasibility of high-frequency ultrasound for seminiferous tubule assessment and correlation of B-mode imaging with pathological findings in the testis in azoospermia.

  Tomoyuki Ohta, Kosuke Kojo, Masahiro Kurobe, Daisuke Numahata, Takayama Tatsuya, Shinya Okada, Teruaki Iwamoto

  Journal of medical ultrasonics (2001)   51 ( 3 ) 465 - 475   2024.07 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  PURPOSE: To determine the feasibility of high-frequency ultrasound (HFUS) for assessing seminiferous tubules and to understand high-resolution B-mode images of the testes in cases of azoospermia. METHODS: We verified how the histopathological images of testicular biopsy specimens can be observed using HFUS images and measurement analysis of seminiferous tubules was performed to 28 testes of 14 cases with azoospermia who underwent preoperative ultrasound and microdissection testicular sperm extraction (micro-TESE). The population consisted of obstructive azoospermia (OA) and non-obstructive azoospermia (NOA), including Sertoli cell-only syndrome (SCOS), and the other pathologies. Statistical verification of differences in seminiferous tubule diameters among preoperative ultrasound examination, ultrasound examination of pathological specimens, and histopathological specimens. We also examined the imagingpathology correlation via a case series presentation, aiming to identify imaging markers of testicular pathology and determine the possibility of predicting each condition. RESULTS: A comparison between HFUS images and histopathology from the same biopsy specimens suggested that ultrasonography could be seen as stereoscopic images due to its significantly greater slice thickness. The diameters of tubules were generally larger in pathological tissues as compared to ultrasonographic findings in OA and SCOS, but not in the other conditions. Comparisons provided insights into the predictability of SCOS and revealed imaging findings such as gaps between tubules and decreased diameter reflective of testicular damage. CONCLUSION: Seminiferous tubules can be observed however the diameter of seminiferous tubules varies in imaging and histopathology depending on the pathology. Imaging findings that reflect testicular damage and the predictability of SCOS were revealed in this study, but further verification is required.

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• [A Case of Proliferative Cystitis Discovered as Protruding Lesion during Inspection of Secondary Infertility].

  Keita Okamoto, Kosuke Kojo, Masahiro Kurobe, Yoshimasa Nakazato, Hiromu Inai, Katsunori Uchida, Jun Miyazaki, Tatsuya Takayama

  Hinyokika kiyo. Acta urologica Japonica   70 ( 6 ) 167 - 171   2024.06 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  A 42-year-old man visited our hospital complaining of secondary infertility. An abdominal ultrasonography screening incidentally revealed a protruding lesion in the bladder. As the lesion extended from the prostatic urethra and bladder neck, there was a possibility of ejaculation dysfunction after resection of the lesion. Therefore, with the patient's informed consent, sperm cryopreservation was conducted for fertility preservation, and subsequently histological examination was performed by partial transurethral resection of bladder tumor. The pathological findings were proliferative cystitis including all three subtypes (glandularis, cystica, and papillary). Cyclooxygenase-2 immunostaining was positive in cytoplasm; weakly positive in cystic and papillary lesions, and strongly positive in glandular lesions. According to a literature review of massive proliferative cystitis, the patient was the 77th case in Japan. Novel postoperative immunological pharmacotherapies with cyclooxygenase-2 inhibitors have been introduced in recent years.

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• Analysis of the correlation between gene copy deletion in the AZFc region and male infertility in Japanese men.

  Yusuke Nakagawa, Atsushi Tada, Kosuke Kojo, Haruki Tsuchiya, Masahiro Kurobe, Masahiro Uchida, Kazumitsu Yamasaki, Teruaki Iwamoto, Youichi Sato

  Reproductive biology   23 ( 1 ) 100728 - 100728   2023.03 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  Deletion of the azoospermia factor c (AZFc), located on the long arm of the Y chromosome, is a cause of male infertility. The structure of the Y chromosome is diversified by the copy number of various genes, such as deleted in azoospermia (DAZ), basic protein Y2, chromodomain Y1, testis-specific transcript Y-linked 4, and Golgi autoantigen golgin subfamily a2 like Y, located in the AZF region. In this study, we investigated the deletion of each gene copy and analyzed its relationship with Japanese male infertility. Deletions of single nucleotide variants of each gene copy in 721 proven fertile men as controls, 139 patients with non-obstructive azoospermia (NOA), and 56 patients with oligozoospermia (OS) were analyzed via polymerase chain reaction-restriction fragment length polymorphism analysis. Their association with infertility was analyzed using logistic regression analysis adjusted for the Y-chromosome haplogroup, D1a2a. Deletions of DAZ/II in the r1 region and DAZ/V in the r1 and r2 regions showed significant associations with NOA (odds ratio [OR] = 4.15, 95 % confidence interval [CI] = 1.18-14.6, P = 0.026; OR = 4.19, 95 % CI = 1.19-14.7, P = 0.025, respectively). They did not show any association with OS. Partial deletion of the AZFc region affects spermatogenesis in Japanese male.

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• A constrained mixture-micturition-growth (CMMG) model of the urinary bladder: Application to partial bladder outlet obstruction (BOO).

  Fangzhou Cheng, Paul N Watton, Giulia Pederzani, Masahiro Kurobe, Ei-Ichiro Takaoka, Chris Chapple, Lori Birder, Naoki Yoshimura, Anne M Robertson

  Journal of the mechanical behavior of biomedical materials   134   105337 - 105337   2022.06 [ Peer Review Accepted ]

  Type of publication: Research paper (scientific journal)

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  We present a constrained mixture-micturition-growth (CMMG) model for the bladder. It simulates bladder mechanics, voiding function (micturition) and tissue adaptations in response to altered biomechanical conditions. The CMMG model is calibrated with both in vivo and in vitro data from healthy male rat urinary bladders (cystometry, bioimaging of wall structure, mechanical testing) and applied to simulate the growth and remodeling (G&R) response to partial bladder outlet obstruction (BOO). The bladder wall is represented as a multi-layered, anisotropic, nonlinear constrained mixture. A short time scale micturition component of the CMMG model accounts for the active and passive mechanics of voiding. Over a second, longer time scale, G&R algorithms for the evolution of both cellular and extracellular constituents act to maintain/restore bladder (homeostatic) functionality. The CMMG model is applied to a spherical membrane model of the BOO bladder utilizing temporal data from an experimental male rodent model to parameterize and then verify the model. Consistent with the experimental studies of BOO, the model predicts: an initial loss of voiding capacity followed by hypertrophy of SMC to restore voiding function; bladder enlargement; collagen remodeling to maintain its role as a protective sheath; and increased voiding duration with lower average flow rate. This CMMG model enables a mechanistic approach for investigating the bladder's structure-function relationship and its adaption in pathological conditions. While the approach is illustrated with a conceptual spherical bladder model, it provides the basis for application of the CMMG model to anatomical geometries. Such a mechanistic approach has promise as an in silico tool for the rational development of new surgical and pharmacological treatments for bladder diseases such as BOO.

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Other Papers 【 display / non-display 】

Other Papers 【 display / non-display 】

• Progressive changes of bladder function, histology, and molecular expression in early and late-phases of partial bladder outlet obstruction in a male rat model(和訳中)

  黒部 匡広, 高岡 栄一郎, 齊藤 徹一, 後藤 大輔, 五十嵐 太郎, 宮崎 淳, 西山 博之, 吉村 直樹

  日本泌尿器科学会総会 ( (一社)日本泌尿器科学会総会事務局 )  109回   ISP03 - 04   2021.12  [Refereed]

   

• 雄ラット膀胱出口部部分閉塞の初期および後期における膀胱機能、組織像および分子発現の経時的変化(Progressive changes of bladder function, histology, and molecular expression in early and late-phases of partial bladder outlet obstruction in a male rat model)

  黒部 匡広, 高岡 栄一郎, 齊藤 徹一, 後藤 大輔, 五十嵐 太郎, 宮崎 淳, 西山 博之, 吉村 直樹

  日本泌尿器科学会総会 ( (一社)日本泌尿器科学会総会事務局 )  109回   ISP03 - 04   2021.12  [Refereed]