Published Papers - Noguchi Hirofumi

Islet Culture/Preservation Before Islet Transplantation.

Noguchi H, Miyagi-Shiohira C, Kurima K, Kobayashi N, Saitoh I, Watanabe M, Noguchi Y, Matsushita M

Cell medicine   8 ( 1-2 ) 25 - 29   2015.12 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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Cryopreservation of Adipose-Derived Mesenchymal Stem Cells.

Miyagi-Shiohira C, Kurima K, Kobayashi N, Saitoh I, Watanabe M, Noguchi Y, Matsushita M, Noguchi H

Cell medicine   8 ( 1-2 ) 3 - 7   2015.12 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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A case report of a type 1 diabetes patient maintaining C-peptide secretion for ten years after islet transplantation using donation after cardiocirculatory death (DCD) donors

Yasuhiro Iwanaga, Shinichi Matsumoto, Teru Okitsu, Hideo Nagata, Hirofumi Noguchi, Yukihide Yonekawa, Michiya Kawaguchi, Takayuki Anazawa, Yoshiya Kawaguchi, Hideaki Okajima, Shinji Uemoto

XENOTRANSPLANTATION ( WILEY-BLACKWELL )  22   S97 - S97   2015.11 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

A CASE REPORT OF A TYPE 1 DIABETES PATIENT MAINTAINING C-PEPTIDE SECRETION FOR TEN YEARS AFTER ISLET TRANSPLANTATION USING DONATION AFTER CARDIOCIRCULATORY DEATH (DCD) DONORS.

Yasuhiro Iwanaga, Shinichi Matsumoto, Teru Okitsu, Hideo Nagata, Hirofumi Noguchi, Yukihide Yonekawa, Michiya Kawaguchi, Takayuki Anazawa, Yoshiya Kawaguchi, Hideaki Okajima, Shinji Uemoto

TRANSPLANTATION ( LIPPINCOTT WILLIAMS & WILKINS )  99 ( 11 ) S158 - S158   2015.11 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

Expression of DNMT1 in neural stem/precursor cells is critical for survival of newly generated neurons in the adult hippocampus.

Noguchi H, Kimura A, Murao N, Matsuda T, Namihira M, Nakashima K

Neuroscience research   95   1 - 11   2015.06 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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Basic and Clinical Science for Organ Biology.

Noguchi H

Cell medicine   7 ( 2 ) 49   2015.02 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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Three-Dimensional In Vitro Hepatic Constructs Formed Using Combinatorial Tapered Stencil for Cluster Culture (TASCL) Device.

Miyamoto Y, Ikeuchi M, Noguchi H, Yagi T, Hayashi S

Cell medicine   7 ( 2 ) 67 - 74   2015.02 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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Potential Factors for the Differentiation of ESCs/iPSCs Into Insulin-Producing Cells.

Tsugata T, Nikoh N, Kin T, Saitoh I, Noguchi Y, Ueki H, Watanabe M, James Shapiro AM, Noguchi H

Cell medicine   7 ( 2 ) 83 - 93   2015.02 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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Induction of tissue-specific stem cells by reprogramming factors, and tissue-specific selection.

Hirofumi Noguchi, Issei Saitoh, Takako Tsugata, Hitomi Kataoka, Masami Watanabe, Yasufumi Noguchi

Cell Death and Differentiation ( Cell Death and Differentiation )  22 ( 1 ) 145 - 155   2015.01 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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再生医療

小林 英司, 野口 洋文

Organ Biology ( 一般社団法人　日本臓器保存生物医学会 )  22 ( 2 ) 134 - 135   2015 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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A New Method for Generating Insulin-Secreting Cells from Human Pancreatic Epithelial Cells After Islet Isolation Transformed by NeuroD1

Masayuki Shimoda, Shuyuan Chen, Hirofumi Noguchi, Morihito Takita, Koji Sugimoto, Takeshi Itoh, Daisuke Chujo, Shuichi Iwahashi, Bashoo Naziruddin, Marlon F. Levy, Shinichi Matsumoto, Paul A. Grayburn

HUMAN GENE THERAPY METHODS ( MARY ANN LIEBERT, INC )  25 ( 3 ) 206 - 219   2014.06 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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The generation of insulin-secreting cells from nonendocrine pancreatic epithelial cells (NEPEC) has been demonstrated for potential clinical use in the treatment of diabetes. However, previous methods either had limited efficacy or required viral vectors, which hinder clinical application. In this study, we aimed to establish an efficient method of insulin-secreting cell generation from NEPEC without viral vectors. We used nonislet fractions from both research-grade human pancreata from brain-dead donors and clinical pancreata after total pancreatectomy with autologous islet transplantation to treat chronic pancreatitis. It is of note that a few islets could be mingled in the nonislet fractions, but their influence could be limited. The NeuroD1 gene was induced into NEPEC using an effective triple lipofection method without viral vectors to generate insulin-secreting cells. The differentiation was promoted by adding a growth factor cocktail into the culture medium. Using the research-grade human pancreata, the effective method showed high efficacy in the differentiation of NEPEC into insulin-positive cells that secreted insulin in response to a glucose challenge and improved diabetes after being transplanted into diabetic athymic mice. Using the clinical pancreata, similar efficacy was obtained, even though those pancreata suffered chronic pancreatitis. In conclusion, our effective differentiation protocol with triple lipofection method enabled us to achieve very efficient insulin-secreting cell generation from human NEPEC without viral vectors. This method offers the potential for supplemental insulin-secreting cell transplantation for both allogeneic and autologous islet transplantation.

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Establishment of a pancreatic stem cell line from fibroblast-derived induced pluripotent stem cells

Takashi Kuise, Hirofumi Noguchi, Hiroshi Tazawa, Takashi Kawai, Masaya Iwamuro, Issei Saitoh, Hitomi Usui Kataoka, Masami Watanabe, Yasufumi Noguchi, Toshiyoshi Fujiwara

BIOMEDICAL ENGINEERING ONLINE ( BIOMED CENTRAL LTD )  13 ( 1 ) 64   2014.05 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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Background: For cell therapies to treat diabetes, it is important to produce a sufficient number of pancreatic endocrine cells that function similarly to primary islets. Induced pluripotent stem (iPS) cells represent a potentially unlimited source of functional pancreatic endocrine cells. However, the use of iPS cells for laboratory studies and cell-based therapies is hampered by their high tumorigenic potential and limited ability to generate pure populations of differentiated cell types in vitro. The purpose of this study was to establish a pancreatic stem cell line from iPS cells derived from mouse fibroblasts. Methods: Mouse iPS cells were induced to differentiate into insulin-producing cells by a multi-step differentiation protocol, which was conducted as described previously with minor modifications. Selection of the pancreatic stem cell was based on morphology and Pdx1 expression. The pancreatic potential of the pancreatic stem cells was evaluated using a reverse transcription PCR, real-time PCR, immunofluorescence, and a glucose challenge test. To assess potential tumorigenicity of the pancreatic stem cells, the cells were injected into the quadriceps femoris muscle of the left hindlimb of nude mice. Results: The iPS-derived pancreatic stem cells expressed the transcription factor -Pdx1- a marker of pancreatic development, and continued to divide actively beyond passage 80. Endocrine cells derived from these pancreatic stem cells expressed insulin and pancreatic genes, and they released insulin in response to glucose stimulation. Mice injected with the pancreatic stem cells did not develop tumors, in contrast to mice injected with an equal number of iPS cells. Conclusion: This strategy provides a new approach for generation of insulin-producing cells that is more efficient and safer than using iPS cells. We believe that this approach will help to develop a patient-specific cell transplantation therapy for diabetes in the near future.

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Comparison of Incubation Solutions Prior to the Purification of Porcine Islet Cells.

Kawai T, Noguchi H, Kuise T, Nakatsuka A, Katayama A, Imagawa N, Kataoka HU, Saitoh I, Noguchi Y, Watanabe M, Fujiwara T

Cell medicine   6 ( 1-2 ) 9 - 14   2013.12 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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Development of Canine Models of Type 1 Diabetes With Partial Pancreatectomy and the Administration of Streptozotocin.

Seita M, Noguchi H, Kubota Y, Kawamoto H, Nakaji S, Kobayashi N, Fujiwara T

Cell medicine   6 ( 1-2 ) 25 - 31   2013.12 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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Comparison of New Preservation Solutions, HN-1 and University of Wisconsin Solution, in Pancreas Preservation for Porcine Islet Isolation.

Katayama A, Noguchi H, Kuise T, Nakatsuka A, Hirota D, Kataoka HU, Kawai T, Inoue K, Imagawa N, Saitoh I, Noguchi Y, Watanabe M, Wada J, Fujiwara T

Cell medicine   6 ( 1-2 ) 3 - 8   2013.12 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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Corrigendum to Embryonic body formation using the tapered soft stencil for cluster culture device [Biomaterials 32 (2011) 3729-3738]

Hiroshi Yukawa, Hirofumi Noguchi, Shuji Hayashi

Biomaterials   34 ( 33 ) 8531   2013.11 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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ER Stress and β-Cell Pathogenesis of Type 1 and Type 2 Diabetes and Islet Transplantation.

Kataoka HU, Noguchi H

Cell medicine   5 ( 2-3 ) 53 - 57   2013.11 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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Culture Conditions for Mouse Pancreatic Stem Cells.

Noguchi H, Saitoh I, Kataoka HU, Watanabe M, Noguchi Y, Fujiwara T

Cell medicine   5 ( 2-3 ) 63 - 68   2013.11 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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Cancer stem cell-like characteristics of a CD133+ subpopulation in the J82 human bladder cancer cell line.

Peng Huang, Masami Watanabe, Haruki Kaku, Hideo Ueki, Hirofumi Noguchi, Morito Sugimoto, Takeshi Hirata, Hiroshi Yamada, Kohji Takei, Shaobo Zheng, Kai Xu, Yasutomo Nasu, Yasuyuki Fujii, Chunxiao Liu, Hiromi Kumon

Molecular and clinical oncology   1 ( 1 ) 180 - 184   2013.01 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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Cancer stem cells (CSCs) are thought to be crucial for understanding the biological roots of cancer, and are of increasing importance as a target for new anticancer agents. According to an expression analysis of the cell surface antigens of various types of cancer, CD133 is considered to be a potential marker of cancer stemness. In this study, a human urinary bladder cancer cell line (J82) was used to analyze the cancer stem cell-like characteristics of CD133+ bladder cancer cells in vitro and in vivo. The CD133 expression in the J82 cells was examined and the cells were immunomagnetically categorized into positive and negative subsets. The CD133- and CD133+ subsets were phenotypically divergent with regard to the cell growth pattern, while CD133+ cells tended to colonize during their growth. In CD133+ cells, the pluripotent stem cell factors Oct-4 and Sox-2 were upregulated, and a statistically significant proliferation increase was observed when compared to CD133- cells. The CD133+ subpopulation was more tolerant to the chemotherapeutic agent cisplatin, and Bacillus Calmette-Guérin (BCG), an agent instilled intravesically to treat bladder cancer. In addition, CD133+ J82 cells were more resistant to radiation treatment when compared to CD133- cells. The in vivo tumorigenesis of the CD133- and CD133+ subsets of J82 cancer cells was also examined by subcutaneously injecting them into nude mice. The tumor growth was more aggressive in the CD133+ subpopulation, showing a significant difference in the tumorigenic potential in these subsets. In conclusion, J82 human bladder cancer cells include CD133- and CD133+ subpopulations, while the CD133 molecule is a potential marker of the potential malignancy of human bladder cancer. In the present study, the CD133+ subpopulation was herein demonstrated to have certain characteristics consistent with those of cancer stem cells.

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A novel gene expression system for detecting viable bladder cancer cells

Hideo Ueki, Masami Watanabe, Haruki Kaku, Peng Huang, Shun-Ai Li, Kazuhiko Ochiai, Takeshi Hirata, Hirofumi Noguchi, Hiroshi Yamada, Kohji Takei, Yasutomo Nasu, Yuji Kashiwakura, Hiromi Kumon

INTERNATIONAL JOURNAL OF ONCOLOGY ( SPANDIDOS PUBL LTD )  41 ( 1 ) 135 - 140   2012.07 [ Peer Review Accepted ]

Type of publication: Research paper (scientific journal)

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A novel transcriptional system was developed that can robustly enhance cancer-specific gene expression. In the system, hTERT promoter-driven gene expression was enhanced by an advanced two-step transcriptional amplification (TSTA). This construct was used to develop a novel system for detection of bladder cancer cells. The current study evaluated the advanced TSTA system by examining the cancer-specific gene transcription in various bladder cancer cell lines. The system significantly enhanced cancer-specific luciferase gene expression in the bladder cancer cell lines in comparison to the previous expression system of one-step or conventional TSTA. The fold gain of the enhancement was significantly correlated to the telomerase activity of the cell lines. A green fluorescent protein (GFP) gene encoding plasmid vector was constructed where hTERT promoter-driving transcription is enhanced by the advanced TSTA to utilize the system for the imaging and detection of viable bladder cancer cells. The advanced TSTA-hTERT-GFP plasmid successfully induced cancer-specific gene expression, showing robust GFP expression in human bladder cancer cell lines, but no visible GFP expression in normal bladder urothelial cells. The control GFP plasm id with a CMV promoter yielded GFP expression in both normal bladder cells and cancer cells. The advanced TSTA-hTERT-GFP plasmid allowed selective visualization of viable human bladder cancer cells in mixed cell culture containing 10- and 100-fold more normal bladder urothelial cells. These findings indicate that the advanced TSTA-hTERT expressional system is a valuable tool for detecting viable bladder cancer cells. The current system can be applied for in vitro detection of bladder cancer cells in urine and other types of cancer cells disseminated in vivo.

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