論文 - 益崎　裕章

A novel insulinotropic mechanism of whole grain-derived γ-oryzanol via the suppression of local dopamine D₂ receptor signalling in mouse islet.

Kozuka C, Sunagawa S, Ueda R, Higa M, Ohshiro Y, Tanaka H, Shimizu-Okabe C, Takayama C, Matsushita M, Tsutsui M, Ishiuchi S, Nakata M, Yada T, Miyazaki JI, Oyadomari S, Shimabukuro M, Masuzaki H

British journal of pharmacology ( British Journal of Pharmacology )  172 ( 18 ) 4519 - 4534   2015年09月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

Background and Purpose gamma-Oryzanol, derived from unrefined rice, attenuated the preference for dietary fat in mice, by decreasing hypothalamic endoplasmic reticulum stress. However, no peripheral mechanisms, whereby -oryzanol could ameliorate glucose dyshomeostasis were explored. Dopamine D-2 receptor signalling locally attenuates insulin secretion in pancreatic islets, presumably via decreased levels of intracellular cAMP. We therefore hypothesized that -oryzanol would improve high-fat diet (HFD)-induced dysfunction of islets through the suppression of local D-2 receptor signalling. Experimental Approach Glucose metabolism and regulation of molecules involved in D-2 receptor signalling in pancreatic islets were investigated in male C57BL/6J mice, fed HFD and treated with gamma-oryzanol . In isolated murine islets and the beta cell line, MIN6 , the effects of gamma-oryzanol on glucose-stimulated insulin secretion (GSIS) was analysed using siRNA for D-2 receptors and a variety of compounds which alter D-2 receptor signalling. Key Results In islets, -oryzanol enhanced GSIS via the activation of the cAMP/PKA pathway. Expression of molecules involved in D-2 receptor signalling was increased in islets from HFD-fed mice, which were reciprocally decreased by -oryzanol. Experiments with siRNA for D-2 receptors and D-2 receptor ligands in vitro suggest that gamma-oryzanol suppressed D-2 receptor signalling and augmented GSIS. Conclusions and Implications gamma-Oryzanol exhibited unique anti-diabetic properties. The unexpected effects of gamma-oryzanol on D-2 receptor signalling in islets may provide a novel; natural food-based, approach to anti-diabetic therapy.

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Risk stratification of coronary artery disease in asymptomatic diabetic subjects using multi-detector computed tomography

M. Shimabukuro, T. Saito, T. Higa, K. Nakamura, H. Masuzaki, M. Sata

Circ J   79   2422 - 2429   2015年09月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

Subclinical Carotid Atherosclerosis Burden in the Japanese: Comparison between Okinawa and Nagano Residents.

Shimabukuro M, Hasegawa Y, Higa M, Amano R, Yamada H, Mizushima S, Masuzaki H, Sata M

Journal of atherosclerosis and thrombosis ( 一般社団法人 日本動脈硬化学会 )  22 ( 8 ) 854 - 68   2015年08月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

<b><i>Aim</i></b><b>: </b>The prevalence of overweight and a change in atherosclerotic lipid profiles may be linked to region-specific differences in atherosclerotic diseases. We evaluated whether the lipid phenotype could be linked to region- and sex-specific differences in the degree of atherosclerosis.<br><b><i>Methods</i></b><b>: </b>Non-diabetic subjects included Okinawa (<i>n</i>=1674) and Nagano (<i>n</i>=1392) residents aged 30–75 years who underwent carotid ultrasonography for the measurement of maximum intima-media thickness (max IMT).<br><b><i>Results</i></b><b>: </b>Average max IMT was higher in Okinawa men and women, and the increase in max IMT with age was enhanced in men. Multiple regression analysis showed that in addition to age and systolic blood pressure, low-density lipoprotein cholesterol and non-high-density lipoprotein cholesterol were IMT determinants only in men for both Okinawa and Nagano. Meanwhile, HDL-cholesterol was a determinant for Okinawa men and women, but not for Nagano men and women.<br><b><i>Conclusions</i></b><b>: </b>This is the first report to show region- and sex-specific differences in the determinants for max IMT in a Japanese population. The evaluation of the relationship between lipid profile patterns and region- and sex-specific differences in carotid atherosclerosis burden may be required.

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Estrogen deficiency heterogeneously affects tissue specific stem cells in mice.

Kitajima Y, Doi H, Ono Y, Urata Y, Goto S, Kitajima M, Miura K, Li TS, Masuzaki H

Scientific reports   5   12861   2015年08月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Successful treatment of toxoplasmic encephalitis diagnosed early by polymerase chain reaction after allogeneic hematopoietic stem cell transplantation: Two case reports and review of the literature

Miyagi T.

Transplant Infectious Disease ( Transplant Infectious Disease )  17 ( 4 ) 593 - 598   2015年08月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Successful treatment of toxoplasmic encephalitis quickly diagnosed by polymerase chain reaction after allogeneic hematopoietic stem cell transplantation: two cases reports and review of the literature

T. Miyagi, H. Itonaga, F. Aosai, J. Taguchi, K. Norose, K. Mochiduki, H. Fujii, A. Furumoto, M. Ohama, K. Karimata, A. Yamanoha, H.Taniguchi, S. Sato, N. Taira, Y. Moriuchi, T. Fukushima, H. Masuzaki, Y. Miyazaki

Transpl Infect Dis   17 ( 4 ) 593 - 598   2015年08月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

Experience with recombinant activated factor VII for severe post-partum hemorrhage in Japan, investigated by Perinatology Committee, Japan Society of Obstetrics and Gynecology.

Murakami M, Kobayashi T, Kubo T, Hata T, Takeda S, Masuzaki H

The journal of obstetrics and gynaecology research   41 ( 8 ) 1161 - 8   2015年08月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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National survey of fibrinogen concentrate usage for post-partum hemorrhage in Japan: investigated by the Perinatology Committee, Japan Society of Obstetrics and Gynecology.

Makino S, Takeda S, Kobayashi T, Murakami M, Kubo T, Hata T, Masuzaki H

The journal of obstetrics and gynaecology research   41 ( 8 ) 1155 - 60   2015年08月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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特集 糖のホメオスターシス-最近の知見 食習慣・食の嗜好性と糖ホメオスターシス

益崎 裕章, 小塚 智沙代

糖尿病診療マスター ( 株式会社医学書院 )  13 ( 5 ) 370 - 376   2015年05月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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17β-estradiol and lipopolysaccharide additively promote pelvic inflammation and growth of endometriosis.

Khan KN, Kitajima M, Inoue T, Fujishita A, Nakashima M, Masuzaki H

Reproductive sciences (Thousand Oaks, Calif.)   22 ( 5 ) 585 - 94   2015年05月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Pregnancy-associated microRNAs in plasma as potential molecular markers of ectopic pregnancy.

Miura K, Higashijima A, Mishima H, Miura S, Kitajima M, Kaneuchi M, Yoshiura K, Masuzaki H

Fertility and sterility   103 ( 5 ) 1202 - 8.e1   2015年05月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Circulating levels of maternal plasma cell-free miR-21 are associated with maternal body mass index and neonatal birth weight.

Miura K, Higashijima A, Hasegawa Y, Abe S, Miura S, Kaneuchi M, Yoshiura K, Masuzaki H

Prenatal diagnosis   35 ( 5 ) 509 - 11   2015年05月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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[Discussion Meeting on Care and Treatment of Obesity Syndrome: Latest Approach through Nutrition, Diets, Exercise, Lifestyle Modification and Psychological Aspects].

Masuzaki H, Yabe D, Onishi Y, Matsuzawa Y, Matsuda Y

Nihon Naika Gakkai zasshi. The Journal of the Japanese Society of Internal Medicine ( 一般社団法人 日本内科学会 )  104 ( 4 ) 748 - 62   2015年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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γ-Oryzanol protects pancreatic β-cells against endoplasmic reticulum stress in male mice.

Kozuka C, Sunagawa S, Ueda R, Higa M, Tanaka H, Shimizu-Okabe C, Ishiuchi S, Takayama C, Matsushita M, Tsutsui M, Miyazaki J, Oyadomari S, Shimabukuro M, Masuzaki H

Endocrinology ( Endocrinology )  156 ( 4 ) 1242 - 50   2015年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

　概要を見る

Endoplasmic reticulum (ER) stress is profoundly involved in dysfunction of beta-cells under high-fat diet and hyperglycemia. Our recent study in mice showed that gamma-oryzanol, a unique component of brown rice, acts as a chemical chaperone in the hypothalamus and improves feeding behavior and diet-induced dysmetabolism. However, the entire mechanism whereby gamma-oryzanol improves glucose metabolism throughout the body still remains unclear. In this context, we tested whether gamma-oryzanol reduces ER stress and improves function and survival of pancreatic beta-cells using murine beta-cell line MIN6. In MIN6 cells with augmented ER stress by tunicamycin, gamma-oryzanol decreased exaggerated expression of ER stress-related genes and phosphorylation of eukaryotic initiation factor-2 alpha, resulting in restoration of glucose-stimulated insulin secretion and prevention of apoptosis. In islets from high-fat diet-fed diabetic mice, oral administration of gamma-oryzanol improved glucose-stimulated insulin secretion on following reduction of exaggerated ER stress and apoptosis. Furthermore, we examined the impact of gamma-oryzanol on low-dose streptozotocin-induced diabetic mice, where exaggerated ER stress and resultant apoptosis in beta-cells were observed. Also in this model, gamma-oryzanol attenuated mRNA level of genes involved in ER stress and apoptotic signaling in islets, leading to amelioration of glucose dysmetabolism. Taken together, our findings demonstrate that gamma-oryzanol directly ameliorates ER stress-induced beta-cell dysfunction and subsequent apoptosis, highlighting usefulness of gamma-oryzanol for the treatment of diabetes mellitus.

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Decreased expression of human heat shock protein 70 in the endometria and pathological lesions of women with adenomyosis and uterine myoma after GnRH agonist therapy.

Khan KN, Kitajima M, Hiraki K, Fujishita A, Nakashima M, Masuzaki H

European journal of obstetrics, gynecology, and reproductive biology   187   6 - 13   2015年04月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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ADIPOQ polymorphisms are associated with insulin resistance in Japanese women

A. Kitamoto, T. Kitamoto, R. So, T. Matsuo, Y. Nakata, H. Hyogo, H. Ochi, T. Nakamura, S. Kamohara, N. Miyatake, K. Kotani, R. Komatsu, N. Itoh, I. Mineo, J. Wada, Y. Ogawa, M. Yoneda, A. Nakajima, T. Funahashi, S. Miyazaki, K. Tokunaga, H. Masuzaki, T. Ueno, K. Chayama, K. Hamaguchi, K. Yamada, T. Hanafusa, S. Oikawa, T. Sakata, K. Tanaka, Y. Matsuzawa, K. Hotta

Endocrine J   62 ( 6 ) 513 - 521   2015年03月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

Vaccination during the 2013-2014 influenza season in pregnant Japanese women.

Yamada T, Abe K, Baba Y, Inubashiri E, Kawabata K, Kubo T, Maegawa Y, Fuchi N, Nomizo M, Shimada M, Shiozaki A, Hamada H, Matsubara S, Akutagawa N, Kataoka S, Maeda M, Masuzaki H, Sagawa N, Nakai A, Saito S, Minakami H

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology   34 ( 3 ) 543 - 8   2015年03月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Effect of caffeine contained in a cup of coffee on microvascular function in healthy subjects.

Noguchi K, Matsuzaki T, Sakanashi M, Hamadate N, Uchida T, Kina-Tanada M, Kubota H, Nakasone J, Sakanashi M, Ueda S, Masuzaki H, Ishiuchi S, Ohya Y, Tsutsui M

Journal of pharmacological sciences ( JAPANESE PHARMACOLOGICAL SOC )  127 ( 2 ) 217 - 22   2015年02月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Recent epidemiological studies have demonstrated that coffee drinking is associated with reduced mortality of cardiovascular disease. However, its precise mechanisms remain to be clarified. In this study, we examined whether single ingestion of caffeine contained in a cup of coffee improves microvascular function in healthy subjects. A double-blind, placebo-controlled, crossover study was performed in 27 healthy volunteers. A cup of either caffeinated or decaffeinated coffee was drunk by the subjects, and reactive hyperemia of finger blood flow was assessed by laser Doppler flowmetry. In an interval of more than 2 days, the same experimental protocol was repeated with another coffee in a crossover manner. Caffeinated coffee intake slightly but significantly elevated blood pressure and decreased finger blood flow as compared with decaffeinated coffee intake. There was no significant difference in heart rate between caffeinated and decaffeinated coffee intake. Importantly, caffeinated coffee intake significantly enhanced post-occlusive reactive hyperemia of finger blood flow, an index of microvascular endothelial function, compared with decaffeinated coffee intake. These results provide the first evidence that caffeine contained in a cup of coffee enhances microvascular function in healthy individuals. (C) 2015 Japanese Pharmacological Society. Production and hosting by Elsevier B.V. All rights reserved.

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Authors' reply.

Nishijima K, Takahashi J, Orisaka M, Kurokawa T, Tamamura C, Yoshida A, Masuzaki H, Sekiya T, Yoshida Y, Kotsuji F

BJOG : an international journal of obstetrics and gynaecology   122 ( 3 ) 448 - 9   2015年02月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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Involvement of hepatocyte growth factor-induced epithelial-mesenchymal transition in human adenomyosis.

Khan KN, Kitajima M, Hiraki K, Fujishita A, Nakashima M, Masuzaki H

Biology of reproduction   92 ( 2 ) 35   2015年02月 [ 査読有り ]

掲載種別: 研究論文（学術雑誌）

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